| Definition | Serratia proteamaculans 568 chromosome, complete genome. |
|---|---|
| Accession | NC_009832 |
| Length | 5,448,853 |
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The map label for this gene is kefA [H]
Identifier: 157370768
GI number: 157370768
Start: 2777601
End: 2780054
Strand: Reverse
Name: kefA [H]
Synonym: Spro_2528
Alternate gene names: 157370768
Gene position: 2780054-2777601 (Counterclockwise)
Preceding gene: 157370770
Following gene: 157370766
Centisome position: 51.02
GC content: 54.6
Gene sequence:
>2454_bases ATGCAAATGCTGAGCACCGAATTTATGTTAAAGCCCCGTCAAATTATTCTTTTCCTGTTCCTGCTGCTTGGCTTGCAGTT GGCGCCGCTGACCAGCGTCGCCGCACAGGATGATGATGTTCCTGTTGTTGAAGCCCCGATAGACCCTGCGGTTGCGCTGA AGAACTACCAAAAGCAGTTGGACAGCATCAAACAGCAGGTCTCCAAGGCCGACAACGATAACCAACTCAGCAAGCTGAAG CTGGCTACCGACGATCTGGCCGCCGGGTTGGATAAACTGGCAACCGATCTACAACCGGCGCAGGAGAAACTGCAGGGGCA ACTTGATGTGCTAGGCCCGCAGCCGGCCGCCGGTTCGATGGCCGAAACCGCCGCCGTGGTGCAGCAGCGTAATACATTGA ATAACAGTAAAAAACAACTGGACGATGCGGTGAAACGCGCCCAGACCATGCGGGCCGGTGCGATTGATCTGGGCCAGCAA ATCAGTGATTTGCGTCGGGTGGCGTTCAAAACCCAATTGGCATTGAATACCGGTAGCGTGCTGGGCAGCAAGTTTTGGTC GCCGGTGGTGCAACCTGCCGCCAGTGACATGCAGCGCCTGGACGAGTTTAATACGGCCATGAAACAGGCCTGGGACACCA GCTGGCAGGAAGAGTGGCGCTATGGCACTCTCACTTTTTTGGTATTGGCGGTGATTATCTGGTCATGGGGGCGTTATTTT TCCGAGCGTTTTCTGGCCTGGGTGAGTATTCGTTTTCTGCCGGATGGCCGTTTACGCCGCAGCTTTATGGCGATAATGAC CGTCACCGTTACGGTTATCACCACCAGTATTGCGCTGAATCTGCTGTATCTCACTTTTACCCGCGTGCAGCCGTTGCCGG GAATGCTGGAAGATTTTGCCGAAGGCTTTTATCGGTTGGGGATCTTCTGCGCACTGATTGCCGGTCTGGGCCGGGCGACG CTGTCTCTTAACCGCCCGTCATGGCGTCTGGGTGCCATGGATGACGCGGTGGCCGCCGGTTTACGCTATTTCTCGCCGCT GCTGGCCGGGTTAGCCTTGGCATTTGGCTCAATTGAGCTTATCAACAACGTGGTGAGTGCCAGTCTGGGCACCACCATTT TCGGCAACGGTCTGGTTGCCGGATTAATCGGTATGGTGCTGTTGGCGATGCCGCTGCGCTCCCAGCGCATTCGCCGCCAA CTGGAGCAGCAGGGTGAGCACGTAGAGAAGCGCTCTACTCTCAGTGGCTTGCTGCACATGAGCACCCTGGTGTGTTCGGT GATAATCCTGTTTTCACTGTTGGTGGGGTATATCGCCTTCGCCCGATTCCTGACCTATCAGGTGATCTGGGTCGCTCTGG TACTGATGGCGTTTTATTTTCTGCTGATGTTCGCCACCGATCTGTGCGGTGCGATATTTTCTTCGCAGACCGCCAGCGGC AAGATGCTTAAAAAGTCCTTCAACTTCAAAGACCGTCATCTGGAGCAAATGGCGATTATCTTCACCGCCATTGCCAAGTG TTCTCTGTTGTTATTAATGATTGTCTCGCTGTTTAACGGCTCGTTCGGCACCACCACGCCCAACTCGTTGCTGGACAAGA TCATTGCCATCCTGAGCGGCGAAGGGCTGCAACGGCTGAATATTGTGCCGGGCAACCTGATTAACGCGGTTATTTGTCTG GCGATCGGTATCTATATTCTGCGGGTGACCCAGCGCTGGCTCAGTCAGGAACTGTTGCCGAAAACCATTTCAGACATTGG TATTCGTGCATCACTGGTCACGCTGTTCAGCAATGTGGGTTACGTGCTGCTGATCCTGATCACCCTGGCGGCTCTGGGCA TCCAGTGGAACAATCTGGCGTGGATCGTCAGTGCGTTGTCGGTGGGTATCGGTTTTGGTCTGCAGGAGATAGTGAAGAAC TTCATTTCCGGCCTGATCCTGCTGACCGAACGGCCGGTAAAAGTGGGCGATATGATCGGTATTGGCGGTGTAGAAGGGGA TGTGCGTCGTATTAACGTGCGCGCTACCGAGATCCAACTCAGTGACCGTTCGACCATGATCGTACCCAACTCGCAGCTGA TTTCGCAAAACGTGCGTAATGCCACCATGGGCAATGCGCAGGGCGTGGTGACCATCGCGCTGACCTTCCCGACGGATATC GATCCGGAGCAGGTACGTAATATCTTGCTGAGTGCTTACCAGGAGTATGAAGCCATTCAGGAAACGCCTGCGCCTTATGT TCGTTTTAGTCAGCTTGGCCCGGACGGCATTATTCTTAGCGTGACCGGTTATGTAGCCAGCCCACGCATGGTGGGTGCCA CCAAAAGCGAACTGCTGTTCAATATCCTCAAGCTGCTGCGCGCGGAGCGGGTCAATATGTCCAGCCCGCAGGAAGTGGTG TTTATGAAGCAACAGGCGAAAAACCTGGCCAACGACGAAGAACAGTCTTCGTAA
Upstream 100 bases:
>100_bases TTCTGAATTTACCCCAAACATCCGAAAACCACGCTTTTCGGGTGTTTGTTACAACCTTGCGGGACGGTAAATACCGGCCC GCTTGTTTCGTCTGTCTATC
Downstream 100 bases:
>100_bases TAATACCCTCTCCCTTGGGAGAGGGTTAGGGTGAGGGGGAACATGCATATGCCCTCACCCCGGCCCTCTCCCACAGGAGA GGGGGCGTTCACGGAAGTGC
Product: mechanosensitive ion channel MscS
Products: NA
Alternate protein names: Protein AefA [H]
Number of amino acids: Translated: 817; Mature: 817
Protein sequence:
>817_residues MQMLSTEFMLKPRQIILFLFLLLGLQLAPLTSVAAQDDDVPVVEAPIDPAVALKNYQKQLDSIKQQVSKADNDNQLSKLK LATDDLAAGLDKLATDLQPAQEKLQGQLDVLGPQPAAGSMAETAAVVQQRNTLNNSKKQLDDAVKRAQTMRAGAIDLGQQ ISDLRRVAFKTQLALNTGSVLGSKFWSPVVQPAASDMQRLDEFNTAMKQAWDTSWQEEWRYGTLTFLVLAVIIWSWGRYF SERFLAWVSIRFLPDGRLRRSFMAIMTVTVTVITTSIALNLLYLTFTRVQPLPGMLEDFAEGFYRLGIFCALIAGLGRAT LSLNRPSWRLGAMDDAVAAGLRYFSPLLAGLALAFGSIELINNVVSASLGTTIFGNGLVAGLIGMVLLAMPLRSQRIRRQ LEQQGEHVEKRSTLSGLLHMSTLVCSVIILFSLLVGYIAFARFLTYQVIWVALVLMAFYFLLMFATDLCGAIFSSQTASG KMLKKSFNFKDRHLEQMAIIFTAIAKCSLLLLMIVSLFNGSFGTTTPNSLLDKIIAILSGEGLQRLNIVPGNLINAVICL AIGIYILRVTQRWLSQELLPKTISDIGIRASLVTLFSNVGYVLLILITLAALGIQWNNLAWIVSALSVGIGFGLQEIVKN FISGLILLTERPVKVGDMIGIGGVEGDVRRINVRATEIQLSDRSTMIVPNSQLISQNVRNATMGNAQGVVTIALTFPTDI DPEQVRNILLSAYQEYEAIQETPAPYVRFSQLGPDGIILSVTGYVASPRMVGATKSELLFNILKLLRAERVNMSSPQEVV FMKQQAKNLANDEEQSS
Sequences:
>Translated_817_residues MQMLSTEFMLKPRQIILFLFLLLGLQLAPLTSVAAQDDDVPVVEAPIDPAVALKNYQKQLDSIKQQVSKADNDNQLSKLK LATDDLAAGLDKLATDLQPAQEKLQGQLDVLGPQPAAGSMAETAAVVQQRNTLNNSKKQLDDAVKRAQTMRAGAIDLGQQ ISDLRRVAFKTQLALNTGSVLGSKFWSPVVQPAASDMQRLDEFNTAMKQAWDTSWQEEWRYGTLTFLVLAVIIWSWGRYF SERFLAWVSIRFLPDGRLRRSFMAIMTVTVTVITTSIALNLLYLTFTRVQPLPGMLEDFAEGFYRLGIFCALIAGLGRAT LSLNRPSWRLGAMDDAVAAGLRYFSPLLAGLALAFGSIELINNVVSASLGTTIFGNGLVAGLIGMVLLAMPLRSQRIRRQ LEQQGEHVEKRSTLSGLLHMSTLVCSVIILFSLLVGYIAFARFLTYQVIWVALVLMAFYFLLMFATDLCGAIFSSQTASG KMLKKSFNFKDRHLEQMAIIFTAIAKCSLLLLMIVSLFNGSFGTTTPNSLLDKIIAILSGEGLQRLNIVPGNLINAVICL AIGIYILRVTQRWLSQELLPKTISDIGIRASLVTLFSNVGYVLLILITLAALGIQWNNLAWIVSALSVGIGFGLQEIVKN FISGLILLTERPVKVGDMIGIGGVEGDVRRINVRATEIQLSDRSTMIVPNSQLISQNVRNATMGNAQGVVTIALTFPTDI DPEQVRNILLSAYQEYEAIQETPAPYVRFSQLGPDGIILSVTGYVASPRMVGATKSELLFNILKLLRAERVNMSSPQEVV FMKQQAKNLANDEEQSS >Mature_817_residues MQMLSTEFMLKPRQIILFLFLLLGLQLAPLTSVAAQDDDVPVVEAPIDPAVALKNYQKQLDSIKQQVSKADNDNQLSKLK LATDDLAAGLDKLATDLQPAQEKLQGQLDVLGPQPAAGSMAETAAVVQQRNTLNNSKKQLDDAVKRAQTMRAGAIDLGQQ ISDLRRVAFKTQLALNTGSVLGSKFWSPVVQPAASDMQRLDEFNTAMKQAWDTSWQEEWRYGTLTFLVLAVIIWSWGRYF SERFLAWVSIRFLPDGRLRRSFMAIMTVTVTVITTSIALNLLYLTFTRVQPLPGMLEDFAEGFYRLGIFCALIAGLGRAT LSLNRPSWRLGAMDDAVAAGLRYFSPLLAGLALAFGSIELINNVVSASLGTTIFGNGLVAGLIGMVLLAMPLRSQRIRRQ LEQQGEHVEKRSTLSGLLHMSTLVCSVIILFSLLVGYIAFARFLTYQVIWVALVLMAFYFLLMFATDLCGAIFSSQTASG KMLKKSFNFKDRHLEQMAIIFTAIAKCSLLLLMIVSLFNGSFGTTTPNSLLDKIIAILSGEGLQRLNIVPGNLINAVICL AIGIYILRVTQRWLSQELLPKTISDIGIRASLVTLFSNVGYVLLILITLAALGIQWNNLAWIVSALSVGIGFGLQEIVKN FISGLILLTERPVKVGDMIGIGGVEGDVRRINVRATEIQLSDRSTMIVPNSQLISQNVRNATMGNAQGVVTIALTFPTDI DPEQVRNILLSAYQEYEAIQETPAPYVRFSQLGPDGIILSVTGYVASPRMVGATKSELLFNILKLLRAERVNMSSPQEVV FMKQQAKNLANDEEQSS
Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form
COG id: COG3264
COG function: function code M; Small-conductance mechanosensitive channel
Gene ontology:
Cell location: Cell inner membrane; Multi-pass membrane protein [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the mscS (TC 1.A.23) family [H]
Homologues:
Organism=Escherichia coli, GI1786670, Length=259, Percent_Identity=31.6602316602317, Blast_Score=137, Evalue=2e-33, Organism=Escherichia coli, GI2367355, Length=509, Percent_Identity=26.3261296660118, Blast_Score=129, Evalue=6e-31, Organism=Escherichia coli, GI1789291, Length=208, Percent_Identity=24.0384615384615, Blast_Score=72, Evalue=2e-13,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR010920 - InterPro: IPR011066 - InterPro: IPR006685 - InterPro: IPR006686 - InterPro: IPR011014 [H]
Pfam domain/function: PF00924 MS_channel [H]
EC number: NA
Molecular weight: Translated: 89769; Mature: 89769
Theoretical pI: Translated: 9.15; Mature: 9.15
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.6 %Cys (Translated Protein) 3.1 %Met (Translated Protein) 3.7 %Cys+Met (Translated Protein) 0.6 %Cys (Mature Protein) 3.1 %Met (Mature Protein) 3.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MQMLSTEFMLKPRQIILFLFLLLGLQLAPLTSVAAQDDDVPVVEAPIDPAVALKNYQKQL CCCCCHHHHCCHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHH DSIKQQVSKADNDNQLSKLKLATDDLAAGLDKLATDLQPAQEKLQGQLDVLGPQPAAGSM HHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCHH AETAAVVQQRNTLNNSKKQLDDAVKRAQTMRAGAIDLGQQISDLRRVAFKTQLALNTGSV HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH LGSKFWSPVVQPAASDMQRLDEFNTAMKQAWDTSWQEEWRYGTLTFLVLAVIIWSWGRYF HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHHHHH SERFLAWVSIRFLPDGRLRRSFMAIMTVTVTVITTSIALNLLYLTFTRVQPLPGMLEDFA HHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH EGFYRLGIFCALIAGLGRATLSLNRPSWRLGAMDDAVAAGLRYFSPLLAGLALAFGSIEL HHHHHHHHHHHHHHHCCHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH INNVVSASLGTTIFGNGLVAGLIGMVLLAMPLRSQRIRRQLEQQGEHVEKRSTLSGLLHM HHHHHHHHHCCHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH STLVCSVIILFSLLVGYIAFARFLTYQVIWVALVLMAFYFLLMFATDLCGAIFSSQTASG HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH KMLKKSFNFKDRHLEQMAIIFTAIAKCSLLLLMIVSLFNGSFGTTTPNSLLDKIIAILSG HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC EGLQRLNIVPGNLINAVICLAIGIYILRVTQRWLSQELLPKTISDIGIRASLVTLFSNVG CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH YVLLILITLAALGIQWNNLAWIVSALSVGIGFGLQEIVKNFISGLILLTERPVKVGDMIG HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEECCCCCCCCCEEE IGGVEGDVRRINVRATEIQLSDRSTMIVPNSQLISQNVRNATMGNAQGVVTIALTFPTDI CCCCCCCCEEEEEEEEEEEECCCCEEECCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCC DPEQVRNILLSAYQEYEAIQETPAPYVRFSQLGPDGIILSVTGYVASPRMVGATKSELLF CHHHHHHHHHHHHHHHHHHHHCCCCCEEHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHH NILKLLRAERVNMSSPQEVVFMKQQAKNLANDEEQSS HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCC >Mature Secondary Structure MQMLSTEFMLKPRQIILFLFLLLGLQLAPLTSVAAQDDDVPVVEAPIDPAVALKNYQKQL CCCCCHHHHCCHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHH DSIKQQVSKADNDNQLSKLKLATDDLAAGLDKLATDLQPAQEKLQGQLDVLGPQPAAGSM HHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCHH AETAAVVQQRNTLNNSKKQLDDAVKRAQTMRAGAIDLGQQISDLRRVAFKTQLALNTGSV HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH LGSKFWSPVVQPAASDMQRLDEFNTAMKQAWDTSWQEEWRYGTLTFLVLAVIIWSWGRYF HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHHHHH SERFLAWVSIRFLPDGRLRRSFMAIMTVTVTVITTSIALNLLYLTFTRVQPLPGMLEDFA HHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH EGFYRLGIFCALIAGLGRATLSLNRPSWRLGAMDDAVAAGLRYFSPLLAGLALAFGSIEL HHHHHHHHHHHHHHHCCHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH INNVVSASLGTTIFGNGLVAGLIGMVLLAMPLRSQRIRRQLEQQGEHVEKRSTLSGLLHM HHHHHHHHHCCHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH STLVCSVIILFSLLVGYIAFARFLTYQVIWVALVLMAFYFLLMFATDLCGAIFSSQTASG HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH KMLKKSFNFKDRHLEQMAIIFTAIAKCSLLLLMIVSLFNGSFGTTTPNSLLDKIIAILSG HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC EGLQRLNIVPGNLINAVICLAIGIYILRVTQRWLSQELLPKTISDIGIRASLVTLFSNVG CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH YVLLILITLAALGIQWNNLAWIVSALSVGIGFGLQEIVKNFISGLILLTERPVKVGDMIG HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEECCCCCCCCCEEE IGGVEGDVRRINVRATEIQLSDRSTMIVPNSQLISQNVRNATMGNAQGVVTIALTFPTDI CCCCCCCCEEEEEEEEEEEECCCCEEECCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCC DPEQVRNILLSAYQEYEAIQETPAPYVRFSQLGPDGIILSVTGYVASPRMVGATKSELLF CHHHHHHHHHHHHHHHHHHHHCCCCCEEHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHH NILKLLRAERVNMSSPQEVVFMKQQAKNLANDEEQSS HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 9278503; 10202137 [H]