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The map label for this gene is pcaK [H]

Identifier: 157370752

GI number: 157370752

Start: 2758258

End: 2759616

Strand: Reverse

Name: pcaK [H]

Synonym: Spro_2512

Alternate gene names: 157370752

Gene position: 2759616-2758258 (Counterclockwise)

Preceding gene: 157370755

Following gene: 157370747

Centisome position: 50.65

GC content: 57.1

Gene sequence:

>1359_bases
GTGGCTTCAAACGAAATTGATGTTCGAACGCTGATCAACGATCGTCCGATATGCGGTTATCAAAAACTGATTATTCTGCT
GGGTTTTATCGTTATTGCGCTGGATGGCTTCGATATTACCCTGATGGGGTTTATTGCTCCTGAGCTGAAAAGCCAGTGGG
GCGTCAGCAATCAGCAACTTGGCATGGTGATTAGCGCCGCGCTGGTTGGCCTGACGATCGGTGCGCTGCTGGCCGGCCCG
CTGGCCGACTGGCTGGGGCGTAAAGTGATCATTATCAACAGCGTGTTCTTTTTTGGCCTCTGGACGCTGGTCACTGCGTT
TTCGCACAATATCGATCAAATGATATTTTTCCGTTTTATGACCGGGTTGGGGCTGGGGGCGGCAATGCCGAACGTCGGCA
CTTTAATCTCCGAATATGCGCCGGAAAAGAAACGTTCCTTCATTATTACCGTGGTGTTTTGCGGCTTTACCTTCGGTGCC
GCGCTGGGCGGGTTCGCGGCGTCATGGGTCATTCCACAAATGGGGTGGCATGCGCTGCTGTTGCTCGGTGGCATATTGCC
GCTGCTGAGCGTTCCGCTGTTGATAGCCAGGCTGCCTGAGTCGGTGAGGTTTTTGGTTACCCGGCGGGCCAGCAATCAAC
GCATTGGCAAAATTGTGGAAAAGCTGGCGCCGGGCAGTACCAATGCCGAAACCCGTTATACGCTGCCGCCACTGGTGGCA
CCTGGCAAGGGAGCCATGCAGATTGTGATCTCGCGGCAGTACCGCCTTGGCAGCCTGATGCTGTGGCTGGGTTATTTCAT
GGCGCTGTTTCTGGTTTATCTGCTGGGCAGTTGGTTACCGACGCTGGTAAAAGGCATCGGCATGAGCGTGGCGCAGGCGG
CGGTGATTACCGCGCTGTACCAGGCCGGCGGTACCTTAGGCTCGCTGTTTGCCGGCTGGCTGATGGATAGGGTGGATCCA
CACCGGGCGCTGGGCATGATTTACCTGCTGGGGGCGGGGCTGACATTGTCGATTGGCCTGGCGTCACACAGCAGCCTGAT
GCTCGGTGTGTTGGCACTGGCCTGTGGTTTTTGCCTGAACGGTGCCAATACCGGCATGAACGCGCTATCGGCGCATTATT
ACCCAACCGAGGCGCGCGCCACCGGGTCGAGCTGGATGCACGGCGTTGGGCGTATTGGTGCCATCCTGAGCGCCTTTGCC
GGTGCGGAAATGCTGGCGTCAGGCTGGGGGTTCGACCGCATTTTTGCCATTATCGCCATTCCGGCGTTGATCACCGCCTG
TGCCATCTTTATCAAAGGTGCCAGAGGGTATAACCGTCAGGCGCAGTTGGTCAGCCGGGAAACCGCTAAAGGCCTTTGA

Upstream 100 bases:

>100_bases
CCGAGCCGCACGGGAGTTCTTACAGTAGACGCCAGAATCTTTAATCTCGGTTCGATTATCGAACATTTGCATTTATTGGC
GGCTGTACCAAGGAAAAATT

Downstream 100 bases:

>100_bases
TCTCACTGATTGAGCCGTTGCAATTGGATTTGATCGGCCTGCCGTGCCTGCTCTGCCAGTGAGGAAAACTGTGCCGGACG
ATAGAATGTTTTGGGCACCG

Product: major facilitator transporter

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MASNEIDVRTLINDRPICGYQKLIILLGFIVIALDGFDITLMGFIAPELKSQWGVSNQQLGMVISAALVGLTIGALLAGP
LADWLGRKVIIINSVFFFGLWTLVTAFSHNIDQMIFFRFMTGLGLGAAMPNVGTLISEYAPEKKRSFIITVVFCGFTFGA
ALGGFAASWVIPQMGWHALLLLGGILPLLSVPLLIARLPESVRFLVTRRASNQRIGKIVEKLAPGSTNAETRYTLPPLVA
PGKGAMQIVISRQYRLGSLMLWLGYFMALFLVYLLGSWLPTLVKGIGMSVAQAAVITALYQAGGTLGSLFAGWLMDRVDP
HRALGMIYLLGAGLTLSIGLASHSSLMLGVLALACGFCLNGANTGMNALSAHYYPTEARATGSSWMHGVGRIGAILSAFA
GAEMLASGWGFDRIFAIIAIPALITACAIFIKGARGYNRQAQLVSRETAKGL

Sequences:

>Translated_452_residues
MASNEIDVRTLINDRPICGYQKLIILLGFIVIALDGFDITLMGFIAPELKSQWGVSNQQLGMVISAALVGLTIGALLAGP
LADWLGRKVIIINSVFFFGLWTLVTAFSHNIDQMIFFRFMTGLGLGAAMPNVGTLISEYAPEKKRSFIITVVFCGFTFGA
ALGGFAASWVIPQMGWHALLLLGGILPLLSVPLLIARLPESVRFLVTRRASNQRIGKIVEKLAPGSTNAETRYTLPPLVA
PGKGAMQIVISRQYRLGSLMLWLGYFMALFLVYLLGSWLPTLVKGIGMSVAQAAVITALYQAGGTLGSLFAGWLMDRVDP
HRALGMIYLLGAGLTLSIGLASHSSLMLGVLALACGFCLNGANTGMNALSAHYYPTEARATGSSWMHGVGRIGAILSAFA
GAEMLASGWGFDRIFAIIAIPALITACAIFIKGARGYNRQAQLVSRETAKGL
>Mature_451_residues
ASNEIDVRTLINDRPICGYQKLIILLGFIVIALDGFDITLMGFIAPELKSQWGVSNQQLGMVISAALVGLTIGALLAGPL
ADWLGRKVIIINSVFFFGLWTLVTAFSHNIDQMIFFRFMTGLGLGAAMPNVGTLISEYAPEKKRSFIITVVFCGFTFGAA
LGGFAASWVIPQMGWHALLLLGGILPLLSVPLLIARLPESVRFLVTRRASNQRIGKIVEKLAPGSTNAETRYTLPPLVAP
GKGAMQIVISRQYRLGSLMLWLGYFMALFLVYLLGSWLPTLVKGIGMSVAQAAVITALYQAGGTLGSLFAGWLMDRVDPH
RALGMIYLLGAGLTLSIGLASHSSLMLGVLALACGFCLNGANTGMNALSAHYYPTEARATGSSWMHGVGRIGAILSAFAG
AEMLASGWGFDRIFAIIAIPALITACAIFIKGARGYNRQAQLVSRETAKGL

Specific function: Transporter for 4-hydroxybenzoate. Also required for chemotaxis to aromatic acids [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=409, Percent_Identity=25.4278728606357, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI213021148, Length=413, Percent_Identity=24.455205811138, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI166064021, Length=365, Percent_Identity=26.5753424657534, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI87081723, Length=421, Percent_Identity=33.4916864608076, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1788074, Length=403, Percent_Identity=27.2952853598015, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87082231, Length=395, Percent_Identity=24.5569620253165, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786229, Length=398, Percent_Identity=22.8643216080402, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87082159, Length=403, Percent_Identity=26.0545905707196, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788068, Length=419, Percent_Identity=24.582338902148, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI87082404, Length=372, Percent_Identity=21.2365591397849, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788509, Length=134, Percent_Identity=27.6119402985075, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1789152, Length=339, Percent_Identity=23.3038348082596, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI32564663, Length=401, Percent_Identity=23.9401496259352, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24640198, Length=466, Percent_Identity=22.9613733905579, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24640196, Length=466, Percent_Identity=22.9613733905579, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24640200, Length=466, Percent_Identity=22.9613733905579, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19922874, Length=206, Percent_Identity=24.7572815533981, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48279; Mature: 48148

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASNEIDVRTLINDRPICGYQKLIILLGFIVIALDGFDITLMGFIAPELKSQWGVSNQQL
CCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCHHH
GMVISAALVGLTIGALLAGPLADWLGRKVIIINSVFFFGLWTLVTAFSHNIDQMIFFRFM
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHH
TGLGLGAAMPNVGTLISEYAPEKKRSFIITVVFCGFTFGAALGGFAASWVIPQMGWHALL
HHCCCCCCCCHHHHHHHHHCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGGILPLLSVPLLIARLPESVRFLVTRRASNQRIGKIVEKLAPGSTNAETRYTLPPLVA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEECCCCCCC
PGKGAMQIVISRQYRLGSLMLWLGYFMALFLVYLLGSWLPTLVKGIGMSVAQAAVITALY
CCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
QAGGTLGSLFAGWLMDRVDPHRALGMIYLLGAGLTLSIGLASHSSLMLGVLALACGFCLN
HCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHC
GANTGMNALSAHYYPTEARATGSSWMHGVGRIGAILSAFAGAEMLASGWGFDRIFAIIAI
CCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
PALITACAIFIKGARGYNRQAQLVSRETAKGL
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
ASNEIDVRTLINDRPICGYQKLIILLGFIVIALDGFDITLMGFIAPELKSQWGVSNQQL
CCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCHHH
GMVISAALVGLTIGALLAGPLADWLGRKVIIINSVFFFGLWTLVTAFSHNIDQMIFFRFM
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHH
TGLGLGAAMPNVGTLISEYAPEKKRSFIITVVFCGFTFGAALGGFAASWVIPQMGWHALL
HHCCCCCCCCHHHHHHHHHCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGGILPLLSVPLLIARLPESVRFLVTRRASNQRIGKIVEKLAPGSTNAETRYTLPPLVA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEECCCCCCC
PGKGAMQIVISRQYRLGSLMLWLGYFMALFLVYLLGSWLPTLVKGIGMSVAQAAVITALY
CCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
QAGGTLGSLFAGWLMDRVDPHRALGMIYLLGAGLTLSIGLASHSSLMLGVLALACGFCLN
HCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHC
GANTGMNALSAHYYPTEARATGSSWMHGVGRIGAILSAFAGAEMLASGWGFDRIFAIIAI
CCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
PALITACAIFIKGARGYNRQAQLVSRETAKGL
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961399 [H]