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The map label for this gene is hycF [H]

Identifier: 157370669

GI number: 157370669

Start: 2662224

End: 2662769

Strand: Reverse

Name: hycF [H]

Synonym: Spro_2429

Alternate gene names: 157370669

Gene position: 2662769-2662224 (Counterclockwise)

Preceding gene: 157370670

Following gene: 157370668

Centisome position: 48.87

GC content: 57.69

Gene sequence:

>546_bases
ATGCTGAAACTGATTAAAAAAGTGCTGAAAACCGGCCCGGCCACCGTCGCCTATCCGAGCCAGCCGTTGGTTGTCGACCC
CAACTTTCGTGGCAAGCCCGAATACAACCCACAGCAATGTATTGCCTGCGGGGCCTGCGCCAACGCTTGCCCATCCAATG
CGTTGAGTATGACCACCGATCTGACCCGTGGCACGGTGCGCTGGCAGCTGTTTCTCGGGCGCTGCATATTCTGCGCCCGC
TGCGAAGAAGTTTGCCCCACCGCCGCCATCCGGCTTTCACAGCAGTTCGAGCTGGCAGTGTGGCGTAAAGAAGACTTATA
CGAGCAGGCCGACTTTGCCATCTGCCATTGCCGTCAATGCGGCAAAGCCTATGCGTCGCAGAAGGAAATTGACTATGCCA
TGGCGCTGCTCGGGCTAAGCAGTACAGAAACCGCCACGCGCCGTACGCAGTTTGAAACCTGCCCGGCGTGCAAACAACAG
CAAAACCTGACTCAGGCCGATCGCATTGACGTTGGCCGCCATTTGACCCGGGAGATCATCTCATGA

Upstream 100 bases:

>100_bases
CGTCGACGTGCGCAAGCGCCAGTCCATCATAGTGCCGTACAAAGAGATTGAGCGTTATGGCATCGAACGGAAAAACAAAC
CGTTCTAACCGGAGCCCATC

Downstream 100 bases:

>100_bases
ATCCGCCACTGATTGGCCCACGCGACGAAAATGGCCTGCCGGTGCCGATCACCCTCGACGAAAGTATCGCCAAGCTGAAA
ACCACCTTACTGCAGGATAT

Product: formate hydrogenlyase complex iron-sulfur subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 6; Hydrogenase-3 component F [H]

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MLKLIKKVLKTGPATVAYPSQPLVVDPNFRGKPEYNPQQCIACGACANACPSNALSMTTDLTRGTVRWQLFLGRCIFCAR
CEEVCPTAAIRLSQQFELAVWRKEDLYEQADFAICHCRQCGKAYASQKEIDYAMALLGLSSTETATRRTQFETCPACKQQ
QNLTQADRIDVGRHLTREIIS

Sequences:

>Translated_181_residues
MLKLIKKVLKTGPATVAYPSQPLVVDPNFRGKPEYNPQQCIACGACANACPSNALSMTTDLTRGTVRWQLFLGRCIFCAR
CEEVCPTAAIRLSQQFELAVWRKEDLYEQADFAICHCRQCGKAYASQKEIDYAMALLGLSSTETATRRTQFETCPACKQQ
QNLTQADRIDVGRHLTREIIS
>Mature_181_residues
MLKLIKKVLKTGPATVAYPSQPLVVDPNFRGKPEYNPQQCIACGACANACPSNALSMTTDLTRGTVRWQLFLGRCIFCAR
CEEVCPTAAIRLSQQFELAVWRKEDLYEQADFAICHCRQCGKAYASQKEIDYAMALLGLSSTETATRRTQFETCPACKQQ
QNLTQADRIDVGRHLTREIIS

Specific function: Probable electron transfer protein for hydrogenase 3 [H]

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=140, Percent_Identity=27.8571428571429, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1789075, Length=180, Percent_Identity=59.4444444444444, Blast_Score=232, Evalue=1e-62,
Organism=Escherichia coli, GI1788833, Length=170, Percent_Identity=50.5882352941176, Blast_Score=138, Evalue=2e-34,
Organism=Escherichia coli, GI1788617, Length=112, Percent_Identity=37.5, Blast_Score=77, Evalue=5e-16,
Organism=Drosophila melanogaster, GI17864306, Length=129, Percent_Identity=29.4573643410853, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 20230; Mature: 20230

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
8.8 %Cys+Met (Translated Protein)
7.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
8.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLIKKVLKTGPATVAYPSQPLVVDPNFRGKPEYNPQQCIACGACANACPSNALSMTTD
CHHHHHHHHHCCCCEEECCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHH
LTRGTVRWQLFLGRCIFCARCEEVCPTAAIRLSQQFELAVWRKEDLYEQADFAICHCRQC
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GKAYASQKEIDYAMALLGLSSTETATRRTQFETCPACKQQQNLTQADRIDVGRHLTREII
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHC
S
C
>Mature Secondary Structure
MLKLIKKVLKTGPATVAYPSQPLVVDPNFRGKPEYNPQQCIACGACANACPSNALSMTTD
CHHHHHHHHHCCCCEEECCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHH
LTRGTVRWQLFLGRCIFCARCEEVCPTAAIRLSQQFELAVWRKEDLYEQADFAICHCRQC
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GKAYASQKEIDYAMALLGLSSTETATRRTQFETCPACKQQQNLTQADRIDVGRHLTREII
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]