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The map label for this gene is hycE [H]

Identifier: 157370670

GI number: 157370670

Start: 2662782

End: 2664485

Strand: Reverse

Name: hycE [H]

Synonym: Spro_2430

Alternate gene names: 157370670

Gene position: 2664485-2662782 (Counterclockwise)

Preceding gene: 157370671

Following gene: 157370669

Centisome position: 48.9

GC content: 59.74

Gene sequence:

>1704_bases
ATGACTAACGAACATCAAGGCCAGCACTATCTGGCGGCGTTGCAGCAACAATTCCCCGCTGCCATGCTGGCGGCAGAATG
GCAAACGGCCGACCAACTGACGGTGACCGTCAAACTCAACGCGCTGCCGGAAGTGGTGGAATGGCTGTATTACCAACAGG
GCGGCTGGTTGTCGGTGCTGTTTGGTAACGACGAACGCACGCTGTGTGGCAATTACGCGCTGTATTACGTGCTGTCGATG
GAGCAAGGCACCAAATGCTGGATCACGGTACGGGCCGAGGTTGACGCCCAAACGCTGGAGTTCCCTTCCGTTACGCCACG
GGTGCCTGCTGCCGTCTGGGGTGAACGCGAAGTGCGCGATATGTATGGCCTGCGCCCGATCGGTTTGCCGGATGAACGCC
GGTTGGTGCTGCCGGACGACTGGCCGGACGATCTCCATCCGTTGCGCAAAGACGCCATGGATTATCGTCAACGGCCCGCG
CCTACCACCGATGTGGAAACCTATCCGTTCCAGAGCCTGGCCGGCAGTAAAGCCAACGTGGTGCCGATCGGCCCGTTGCA
TATCACCTCGGATGAACCCGGGCATTTCCGCCTGTTCGTCGACGGCGAAGACATCATTGATGCCGACTATCGGCTGTTCT
ACGTCCACCGTGGCATGGAGAAACTGGCCGAAACCCGCATGGGTTACAACGAAGTGACCTTCCTGTCGGACCGGGTGTGC
GGCATTTGCGGTTTTACCCACAGCGTGGCCTACACCAGTTCGGTGGAGAACGCGATGGGCATCCAGGTGCCGGAACGTGC
CCAGATGATCCGCTCAGTGCTGCTGGAAGTGGAACGCCTGCACAGTCATCTGCTGAATCTGGGGCTGGCCTGCCATTTCG
TCGGTTTCGACTCCGGTTTTATGCAGTTCTTCCGGGTGCGCGAACAGTCGATGAAGATGGCGGAGATCCTGACCGGTGCA
CGCAAAACCTATGGTTTGAACCTGATTGGCGGCATCCGCCGCGACATACTGAAAGACGACATGCTGCAAACCCTGCGGCT
GGCGGCGCAAATGCGCAGTGAGTTGGTGGATCTGGTCGATATCTTGCTCAGCACGCCGAATACCGAACAGCGCAGCGTCG
GGGTTGGCCGGCTCGATCCGCAGGTGGCTCGCGACTACAGCAACGTCGGCCCGATGATCCGCGGCAGCGGCCACCGCCGC
GATACCCGTATCGACCATCCGTTTGCCGGTTACGCCAAACTGCCGGTGGAACTGTGCGTAGAGGACGGCTGCGACGTCTA
CTCCCGGCTTAAGGTGCGTATTCACGAAGTGTTCAACTCGCTCGGCATCATCGAGTTCGGCCTGCAAAGCCTGCCGAGCG
GGCCGCTGGCGGTGGATGGGTTCACCTATATTCCGCACCGCTTTGCCCTGGGCTTTGCCGAAGCGCCGCGCGGTGACGAT
ATTCACTGGAGCATGACCGGCGACAACCAAAAACTGTTCCGCTGGCGCTGCCGTGCCGCGACCTACGCCAATTGGCCAAC
CCTGCGTTACATGCTGCGCGGCAACACGGTTTCCGACGCTCCGCTGATCATCGGCAGCCTGGATCCCTGCTACTCCTGTA
CCGATCGCATGACCATCGTCGACGTGCGCAAGCGCCAGTCCATCATAGTGCCGTACAAAGAGATTGAGCGTTATGGCATC
GAACGGAAAAACAAACCGTTCTAA

Upstream 100 bases:

>100_bases
CTCAAGACGGGCCGTACCACCTGGGCCGGTTTTGGGCTGGCTTTTCTGGCGCTGGTTTCCTGGCTGGTCGTCGGCTGAAC
AATGATTAAGGCAATCAATA

Downstream 100 bases:

>100_bases
CCGGAGCCCATCATGCTGAAACTGATTAAAAAAGTGCTGAAAACCGGCCCGGCCACCGTCGCCTATCCGAGCCAGCCGTT
GGTTGTCGACCCCAACTTTC

Product: NADH dehydrogenase (ubiquinone) 30 kDa subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 5; Hydrogenase-3 component E [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MTNEHQGQHYLAALQQQFPAAMLAAEWQTADQLTVTVKLNALPEVVEWLYYQQGGWLSVLFGNDERTLCGNYALYYVLSM
EQGTKCWITVRAEVDAQTLEFPSVTPRVPAAVWGEREVRDMYGLRPIGLPDERRLVLPDDWPDDLHPLRKDAMDYRQRPA
PTTDVETYPFQSLAGSKANVVPIGPLHITSDEPGHFRLFVDGEDIIDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRVC
GICGFTHSVAYTSSVENAMGIQVPERAQMIRSVLLEVERLHSHLLNLGLACHFVGFDSGFMQFFRVREQSMKMAEILTGA
RKTYGLNLIGGIRRDILKDDMLQTLRLAAQMRSELVDLVDILLSTPNTEQRSVGVGRLDPQVARDYSNVGPMIRGSGHRR
DTRIDHPFAGYAKLPVELCVEDGCDVYSRLKVRIHEVFNSLGIIEFGLQSLPSGPLAVDGFTYIPHRFALGFAEAPRGDD
IHWSMTGDNQKLFRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTIVDVRKRQSIIVPYKEIERYGI
ERKNKPF

Sequences:

>Translated_567_residues
MTNEHQGQHYLAALQQQFPAAMLAAEWQTADQLTVTVKLNALPEVVEWLYYQQGGWLSVLFGNDERTLCGNYALYYVLSM
EQGTKCWITVRAEVDAQTLEFPSVTPRVPAAVWGEREVRDMYGLRPIGLPDERRLVLPDDWPDDLHPLRKDAMDYRQRPA
PTTDVETYPFQSLAGSKANVVPIGPLHITSDEPGHFRLFVDGEDIIDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRVC
GICGFTHSVAYTSSVENAMGIQVPERAQMIRSVLLEVERLHSHLLNLGLACHFVGFDSGFMQFFRVREQSMKMAEILTGA
RKTYGLNLIGGIRRDILKDDMLQTLRLAAQMRSELVDLVDILLSTPNTEQRSVGVGRLDPQVARDYSNVGPMIRGSGHRR
DTRIDHPFAGYAKLPVELCVEDGCDVYSRLKVRIHEVFNSLGIIEFGLQSLPSGPLAVDGFTYIPHRFALGFAEAPRGDD
IHWSMTGDNQKLFRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTIVDVRKRQSIIVPYKEIERYGI
ERKNKPF
>Mature_566_residues
TNEHQGQHYLAALQQQFPAAMLAAEWQTADQLTVTVKLNALPEVVEWLYYQQGGWLSVLFGNDERTLCGNYALYYVLSME
QGTKCWITVRAEVDAQTLEFPSVTPRVPAAVWGEREVRDMYGLRPIGLPDERRLVLPDDWPDDLHPLRKDAMDYRQRPAP
TTDVETYPFQSLAGSKANVVPIGPLHITSDEPGHFRLFVDGEDIIDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRVCG
ICGFTHSVAYTSSVENAMGIQVPERAQMIRSVLLEVERLHSHLLNLGLACHFVGFDSGFMQFFRVREQSMKMAEILTGAR
KTYGLNLIGGIRRDILKDDMLQTLRLAAQMRSELVDLVDILLSTPNTEQRSVGVGRLDPQVARDYSNVGPMIRGSGHRRD
TRIDHPFAGYAKLPVELCVEDGCDVYSRLKVRIHEVFNSLGIIEFGLQSLPSGPLAVDGFTYIPHRFALGFAEAPRGDDI
HWSMTGDNQKLFRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTIVDVRKRQSIIVPYKEIERYGIE
RKNKPF

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=409, Percent_Identity=25.9168704156479, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI260898743, Length=399, Percent_Identity=25.8145363408521, Blast_Score=113, Evalue=4e-25,
Organism=Escherichia coli, GI1789076, Length=568, Percent_Identity=81.8661971830986, Blast_Score=994, Evalue=0.0,
Organism=Escherichia coli, GI1788832, Length=566, Percent_Identity=71.0247349823322, Blast_Score=861, Evalue=0.0,
Organism=Escherichia coli, GI145693162, Length=606, Percent_Identity=25.2475247524752, Blast_Score=162, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI17568379, Length=384, Percent_Identity=25, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17555284, Length=384, Percent_Identity=24.7395833333333, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI221459469, Length=387, Percent_Identity=25.0645994832041, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24638644, Length=381, Percent_Identity=26.509186351706, Blast_Score=135, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 64394; Mature: 64263

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00535 COMPLEX1_49K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNEHQGQHYLAALQQQFPAAMLAAEWQTADQLTVTVKLNALPEVVEWLYYQQGGWLSVL
CCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHCCCCEEEEE
FGNDERTLCGNYALYYVLSMEQGTKCWITVRAEVDAQTLEFPSVTPRVPAAVWGEREVRD
ECCCCCEEECCEEEEEEEEECCCCEEEEEEEEECCCCEECCCCCCCCCCHHHCCCHHHHH
MYGLRPIGLPDERRLVLPDDWPDDLHPLRKDAMDYRQRPAPTTDVETYPFQSLAGSKANV
HHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCE
VPIGPLHITSDEPGHFRLFVDGEDIIDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRVC
EECCCEEECCCCCCEEEEEECCCCEECCCEEEEEEHHHHHHHHHHHCCCCCEEEHHCCHH
GICGFTHSVAYTSSVENAMGIQVPERAQMIRSVLLEVERLHSHLLNLGLACHFVGFDSGF
HHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHH
MQFFRVREQSMKMAEILTGARKTYGLNLIGGIRRDILKDDMLQTLRLAAQMRSELVDLVD
HHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILLSTPNTEQRSVGVGRLDPQVARDYSNVGPMIRGSGHRRDTRIDHPFAGYAKLPVELCV
HHHCCCCCHHHCCCCCCCCHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCHHCCHHHHH
EDGCDVYSRLKVRIHEVFNSLGIIEFGLQSLPSGPLAVDGFTYIPHRFALGFAEAPRGDD
HCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEECCEECCCHHHHCCCCCCCCCCC
IHWSMTGDNQKLFRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTIV
EEEEEECCCHHHHHEEHHHCCCCCCCCEEEEEECCCCCCCCEEEECCCCHHCCCCCEEEE
DVRKRQSIIVPYKEIERYGIERKNKPF
EECCCCCEECCHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TNEHQGQHYLAALQQQFPAAMLAAEWQTADQLTVTVKLNALPEVVEWLYYQQGGWLSVL
CCCCCCHHHHHHHHHHCCHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHCCCCEEEEE
FGNDERTLCGNYALYYVLSMEQGTKCWITVRAEVDAQTLEFPSVTPRVPAAVWGEREVRD
ECCCCCEEECCEEEEEEEEECCCCEEEEEEEEECCCCEECCCCCCCCCCHHHCCCHHHHH
MYGLRPIGLPDERRLVLPDDWPDDLHPLRKDAMDYRQRPAPTTDVETYPFQSLAGSKANV
HHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCE
VPIGPLHITSDEPGHFRLFVDGEDIIDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRVC
EECCCEEECCCCCCEEEEEECCCCEECCCEEEEEEHHHHHHHHHHHCCCCCEEEHHCCHH
GICGFTHSVAYTSSVENAMGIQVPERAQMIRSVLLEVERLHSHLLNLGLACHFVGFDSGF
HHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHH
MQFFRVREQSMKMAEILTGARKTYGLNLIGGIRRDILKDDMLQTLRLAAQMRSELVDLVD
HHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILLSTPNTEQRSVGVGRLDPQVARDYSNVGPMIRGSGHRRDTRIDHPFAGYAKLPVELCV
HHHCCCCCHHHCCCCCCCCHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCHHCCHHHHH
EDGCDVYSRLKVRIHEVFNSLGIIEFGLQSLPSGPLAVDGFTYIPHRFALGFAEAPRGDD
HCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEECCEECCCHHHHCCCCCCCCCCC
IHWSMTGDNQKLFRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTIV
EEEEEECCCHHHHHEEHHHCCCCCCCCEEEEEECCCCCCCCEEEECCCCHHCCCCCEEEE
DVRKRQSIIVPYKEIERYGIERKNKPF
EECCCCCEECCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503; 8125094 [H]