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The map label for this gene is hycG [H]

Identifier: 157370668

GI number: 157370668

Start: 2661430

End: 2662227

Strand: Reverse

Name: hycG [H]

Synonym: Spro_2428

Alternate gene names: 157370668

Gene position: 2662227-2661430 (Counterclockwise)

Preceding gene: 157370669

Following gene: 157370667

Centisome position: 48.86

GC content: 59.65

Gene sequence:

>798_bases
ATGAATCCGCCACTGATTGGCCCACGCGACGAAAATGGCCTGCCGGTGCCGATCACCCTCGACGAAAGTATCGCCAAGCT
GAAAACCACCTTACTGCAGGATATCCGCCGCTCGGCCTATGTTTATCGCGTGGACTGCGGCGGGTGCAATGGCTGCGAAA
TTGAGATTTTCGCCGCCGTTTCCCCGTTGTTCGACGCCGAACGCTTCGGTATCAAAGTGGTTCCTTCACCACGCCATGCC
GATATTTTGCTGTTTACCGGGGCGGTCACCCGCGCCATGCGTATCCCGGCGATCCGCGCCTGGCAGGCCTCGCCCGATCC
GAAAATCAGCATCTCTTACGGTGCCTGCGGCAACAGCGGTGGCATTTTCCACGATCTCTACTGCGTATGGGGCGGCACCG
ATCGCATCGTACCGGTTGATGTCTATATCCCCGGCTGCCCCCCGACGCCGGCCGCCACCATTTATGGCTTTGCCATGGCA
CTCGGCCTGTTGGATCAGAAAATCAAAGGCCGTCAGCATGACGAAACCCAGGGCGAGGCCACCCGCCTGTTGCACCCGGC
ATTACCTCAGCCGCTACGGGTGCTGCTGGATCGCGAAGCCCGGCGGATGGCCGGTTATCGCTATGGCCGCCAGATCGCCG
ACCAGTTTATGCAACTGTTGGCCGCAGACAGCCCATTGCCGGTCGAACAGCGCATTAGCGCTTACCTGCAACAGCAGGAC
GATATGCGTTTAAGCGAGATTGTCGGCAATCTGCTGGGGATCTATCAACGGGTACTGCGGGGGGAACTGCGACTATGA

Upstream 100 bases:

>100_bases
GTACGCAGTTTGAAACCTGCCCGGCGTGCAAACAACAGCAAAACCTGACTCAGGCCGATCGCATTGACGTTGGCCGCCAT
TTGACCCGGGAGATCATCTC

Downstream 100 bases:

>100_bases
GCCACTCCCATGCCCAGGTGGTGTTCTACTCCCTGAGCCGCAAATTCGTGCAAGAGAAAAAGGCCCCGCCGAAAGCCCAG
GAGGTGATCTATTACAGCCT

Product: NADH ubiquinone oxidoreductase 20 kDa subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MNPPLIGPRDENGLPVPITLDESIAKLKTTLLQDIRRSAYVYRVDCGGCNGCEIEIFAAVSPLFDAERFGIKVVPSPRHA
DILLFTGAVTRAMRIPAIRAWQASPDPKISISYGACGNSGGIFHDLYCVWGGTDRIVPVDVYIPGCPPTPAATIYGFAMA
LGLLDQKIKGRQHDETQGEATRLLHPALPQPLRVLLDREARRMAGYRYGRQIADQFMQLLAADSPLPVEQRISAYLQQQD
DMRLSEIVGNLLGIYQRVLRGELRL

Sequences:

>Translated_265_residues
MNPPLIGPRDENGLPVPITLDESIAKLKTTLLQDIRRSAYVYRVDCGGCNGCEIEIFAAVSPLFDAERFGIKVVPSPRHA
DILLFTGAVTRAMRIPAIRAWQASPDPKISISYGACGNSGGIFHDLYCVWGGTDRIVPVDVYIPGCPPTPAATIYGFAMA
LGLLDQKIKGRQHDETQGEATRLLHPALPQPLRVLLDREARRMAGYRYGRQIADQFMQLLAADSPLPVEQRISAYLQQQD
DMRLSEIVGNLLGIYQRVLRGELRL
>Mature_265_residues
MNPPLIGPRDENGLPVPITLDESIAKLKTTLLQDIRRSAYVYRVDCGGCNGCEIEIFAAVSPLFDAERFGIKVVPSPRHA
DILLFTGAVTRAMRIPAIRAWQASPDPKISISYGACGNSGGIFHDLYCVWGGTDRIVPVDVYIPGCPPTPAATIYGFAMA
LGLLDQKIKGRQHDETQGEATRLLHPALPQPLRVLLDREARRMAGYRYGRQIADQFMQLLAADSPLPVEQRISAYLQQQD
DMRLSEIVGNLLGIYQRVLRGELRL

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=163, Percent_Identity=36.8098159509202, Blast_Score=105, Evalue=5e-23,
Organism=Escherichia coli, GI1789074, Length=247, Percent_Identity=72.0647773279352, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1788834, Length=245, Percent_Identity=64.0816326530612, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI1788624, Length=122, Percent_Identity=40.9836065573771, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=128, Percent_Identity=38.28125, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI18859983, Length=123, Percent_Identity=40.650406504065, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24642371, Length=123, Percent_Identity=40.650406504065, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24651058, Length=123, Percent_Identity=42.2764227642276, Blast_Score=102, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 29281; Mature: 29281

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPPLIGPRDENGLPVPITLDESIAKLKTTLLQDIRRSAYVYRVDCGGCNGCEIEIFAAV
CCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCEEEEEEC
SPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRIPAIRAWQASPDPKISISYGACGNSG
CCHHHHHHCCEEEECCCCCCEEEEEEHHHHHHHHCCCCEEECCCCCCEEEEEECCCCCCC
GIFHDLYCVWGGTDRIVPVDVYIPGCPPTPAATIYGFAMALGLLDQKIKGRQHDETQGEA
CCEEEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
TRLLHPALPQPLRVLLDREARRMAGYRYGRQIADQFMQLLAADSPLPVEQRISAYLQQQD
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
DMRLSEIVGNLLGIYQRVLRGELRL
CCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNPPLIGPRDENGLPVPITLDESIAKLKTTLLQDIRRSAYVYRVDCGGCNGCEIEIFAAV
CCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCEEEEEEC
SPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRIPAIRAWQASPDPKISISYGACGNSG
CCHHHHHHCCEEEECCCCCCEEEEEEHHHHHHHHCCCCEEECCCCCCEEEEEECCCCCCC
GIFHDLYCVWGGTDRIVPVDVYIPGCPPTPAATIYGFAMALGLLDQKIKGRQHDETQGEA
CCEEEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
TRLLHPALPQPLRVLLDREARRMAGYRYGRQIADQFMQLLAADSPLPVEQRISAYLQQQD
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
DMRLSEIVGNLLGIYQRVLRGELRL
CCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]