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The map label for this gene is hypF [H]

Identifier: 157370663

GI number: 157370663

Start: 2655284

End: 2657581

Strand: Reverse

Name: hypF [H]

Synonym: Spro_2423

Alternate gene names: 157370663

Gene position: 2657581-2655284 (Counterclockwise)

Preceding gene: 157370664

Following gene: 157370662

Centisome position: 48.77

GC content: 62.01

Gene sequence:

>2298_bases
GTGTCCCATTCAGGATTACTGCTGCGCATCAGCGGCAAGGTACAGGGCGTCGGCTTCCGGCCTTTTGTCTGGCAACTGGC
CGAACAGCTCAACCTGCGTGGTGAAGTCTGGAACGACAGCGCCGGGGTGGAAATTCGCCTGGTGCAACCTGTCGATCTTG
AGATACTCCTGCACGTGTTACATCACCAGGCGCCACCGTTGGCACAAATCGACCGCATTCAGCAGCGTTCGTTTACCTGG
TTGCAAGCCCCCACCGATTTCAGTATCCGCCCCAGCCAAAGTGGCGCCATGGCAACCCAGATCGTGCCGGATGCCGCGAC
CTGCCCACAGTGTTTGCGGGAAATGAACGATCCGCAGGATCGGCGTTACCGTTATCCCTTTATCAACTGCACCCATTGCG
GCCCGCGCTTTACCATTATTCGCGCCATGCCATACGATCGTCCGGCAACCAGCATGGCGCCCTTCACTCTCTGTCCGCAC
TGCGCCTGGGAATACCATTCTGCGCAGGACCGACGTTTTCATGCCCAGCCGGTGGCCTGTAGCGATTGCGGCCCACAGTT
GTTTAGCTGTCTGGCGCAAGGGCAGCCACAGCATCAGGGGGATGAAGCGTTGCAACAGGCCGTTGCTGCGTTGGCTGCCG
GGCAGATTGTGGCGATCAAAGGCCTGGGCGGTTTTCACCTGGCCTGCGACGCCACCAACACTCAGGCCGTCGAACGGCTG
CGCCTGCGTAAACGGCGGCCGGGCAAACCACTGGCGGTGATGCTGCCGGATGCCGGCTGGCTGGTACGCTGCGTAAGTGG
CGTTAGCCTGGCGCAATTACAGGCATTGTTAACTTCACCCGCCGCGCCTGTGGTGCTGGTCCCTCAATCCCCTTACTCGC
CGTTATCTGCCGCCGTGGCTCCAGGGTTGGATGAAGTCGGCCTGATGTTGCCTGCCAACCCGATTCAACATTTATTGATG
CAGGCGATACAGCGCCCCCTGGTGATGACCTCAGGCAATGCCGCTGGCCGTGCTCCGGCGTTAACCAATGAGCAGGCATT
GGCTGATTTGGTCGACATTGCCGATCTGTGGCTGATGCACGATCGCGAGATCTTGCAACGCGCAGACGACTCGCTGGTGC
GTTTGCTCCCCAAAGGCCATGAAATGCTGCGCCGGGCGCGCGGGTTCGTTCCCGACGCCCTGCCGCTGCCGCCCGGTTTC
CCTGCTCAGCAGGCCTTGTTGGCGGTGGGCGGCGATCGCAAAAATACCTTTTGCCTGGTTCAGGGAAATCAGGCCCTGCT
CGGCCCGCACTTTGGCGATTTGGAGCAAGAGGATATTCAGCAACAGTGGCAACAGGCCATCAGGCACTTCACCGGGTTAT
ACCATTGCCAGCCGCAGCGGCTGGCCAGCGATGCCCATCCCGGTTATGTCAGTCACCAATGGGCGGCCCGGCAGTCACTG
CCGGTGACCCCGGTGCTGCATCATCATGCGCATCTGGCCGCTTGCCTGGCCGAGCACCGTTGGCCACTGGAAGGCGGTGC
CGTGATCGGCCTGGCGCTGGATGGTCTCGGTTATGGTCAGGGCGGCGCCCTGTGGGGCGGCGAGTGTCTGCTGGTTGATT
ACCGCCGCTGTCGTCATCTGGGGGGATTGCCCGCGGTGGCGCTGCCAGGTGGCGATCTTGCGGCTCAGCAACCCTGGCGC
AATCAGTTGGCGCACTTCGCGCGCTTCGTACCGGACTGGCAAAGCTACCCTCAGGCCGAACGGCTGTTGGCTTCCCCATG
GCGACCGTTGCTGCAAGCGATTAAGGCCGGTATTAATGCCCCGCTGGCCTCCTCCTGTGGCCGCTTGTTTGATGCTGCCG
CCGCCACCCTGGGCTGTGCCCCGGCACAACAAAGTTGGGAAGGCGAAGCGGCCAGCCGGTTTGAAGCGCTGGCGCACCAA
AGTGGGCCTTGCCCACACCCGGTGACATTGTCACTGACAGACGAGCAATTTGATCTTGCCGCGTTCTGGCAACGCTGGCT
GGCCTGGCAAGCGCCCGCCCCGCAACAGGCCTGGGCCTTTCACGATGCGCTGGCGCAGGGTTTTGCCAGTCTGGCCAAGC
ATTTTGCCCTGCAGTATGGCATTGGCCATGTAGCGTTGAGCGGCGGTGTGCTGCATAACCGCCTGCTGCGTCAACGCCTG
CAGCACCATCTGGCCCCTTTGCAGATACTGCTGCCTCAGCAACTGCCTGCAGGTGATGGCGGGCTGGCGCTTGGCCAGGC
GCTGGTCGCTTGCGCCCAACATTCTCCGGCTTCTTCTTCCACCTTTGACAGGACCTGA

Upstream 100 bases:

>100_bases
AAGTTGCACTGTCACTTTGTAGAGGGGCGAAGGAATTCGCCCCGATTCACCCGGGCGCAGCATACAGCGCCCCTACAAAA
ACCACAGAGAGTAATAAAAA

Downstream 100 bases:

>100_bases
CATGGTAAACCGCGAGTTTTTCCACACCCATACCCGCGCCTTTTGGCTATTACTGGCGCTGGTGGCGCTAAACCTGTTGG
CCTGGGGAGCGGCTTTTGCC

Product: (NiFe) hydrogenase maturation protein HypF

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 765; Mature: 764

Protein sequence:

>765_residues
MSHSGLLLRISGKVQGVGFRPFVWQLAEQLNLRGEVWNDSAGVEIRLVQPVDLEILLHVLHHQAPPLAQIDRIQQRSFTW
LQAPTDFSIRPSQSGAMATQIVPDAATCPQCLREMNDPQDRRYRYPFINCTHCGPRFTIIRAMPYDRPATSMAPFTLCPH
CAWEYHSAQDRRFHAQPVACSDCGPQLFSCLAQGQPQHQGDEALQQAVAALAAGQIVAIKGLGGFHLACDATNTQAVERL
RLRKRRPGKPLAVMLPDAGWLVRCVSGVSLAQLQALLTSPAAPVVLVPQSPYSPLSAAVAPGLDEVGLMLPANPIQHLLM
QAIQRPLVMTSGNAAGRAPALTNEQALADLVDIADLWLMHDREILQRADDSLVRLLPKGHEMLRRARGFVPDALPLPPGF
PAQQALLAVGGDRKNTFCLVQGNQALLGPHFGDLEQEDIQQQWQQAIRHFTGLYHCQPQRLASDAHPGYVSHQWAARQSL
PVTPVLHHHAHLAACLAEHRWPLEGGAVIGLALDGLGYGQGGALWGGECLLVDYRRCRHLGGLPAVALPGGDLAAQQPWR
NQLAHFARFVPDWQSYPQAERLLASPWRPLLQAIKAGINAPLASSCGRLFDAAAATLGCAPAQQSWEGEAASRFEALAHQ
SGPCPHPVTLSLTDEQFDLAAFWQRWLAWQAPAPQQAWAFHDALAQGFASLAKHFALQYGIGHVALSGGVLHNRLLRQRL
QHHLAPLQILLPQQLPAGDGGLALGQALVACAQHSPASSSTFDRT

Sequences:

>Translated_765_residues
MSHSGLLLRISGKVQGVGFRPFVWQLAEQLNLRGEVWNDSAGVEIRLVQPVDLEILLHVLHHQAPPLAQIDRIQQRSFTW
LQAPTDFSIRPSQSGAMATQIVPDAATCPQCLREMNDPQDRRYRYPFINCTHCGPRFTIIRAMPYDRPATSMAPFTLCPH
CAWEYHSAQDRRFHAQPVACSDCGPQLFSCLAQGQPQHQGDEALQQAVAALAAGQIVAIKGLGGFHLACDATNTQAVERL
RLRKRRPGKPLAVMLPDAGWLVRCVSGVSLAQLQALLTSPAAPVVLVPQSPYSPLSAAVAPGLDEVGLMLPANPIQHLLM
QAIQRPLVMTSGNAAGRAPALTNEQALADLVDIADLWLMHDREILQRADDSLVRLLPKGHEMLRRARGFVPDALPLPPGF
PAQQALLAVGGDRKNTFCLVQGNQALLGPHFGDLEQEDIQQQWQQAIRHFTGLYHCQPQRLASDAHPGYVSHQWAARQSL
PVTPVLHHHAHLAACLAEHRWPLEGGAVIGLALDGLGYGQGGALWGGECLLVDYRRCRHLGGLPAVALPGGDLAAQQPWR
NQLAHFARFVPDWQSYPQAERLLASPWRPLLQAIKAGINAPLASSCGRLFDAAAATLGCAPAQQSWEGEAASRFEALAHQ
SGPCPHPVTLSLTDEQFDLAAFWQRWLAWQAPAPQQAWAFHDALAQGFASLAKHFALQYGIGHVALSGGVLHNRLLRQRL
QHHLAPLQILLPQQLPAGDGGLALGQALVACAQHSPASSSTFDRT
>Mature_764_residues
SHSGLLLRISGKVQGVGFRPFVWQLAEQLNLRGEVWNDSAGVEIRLVQPVDLEILLHVLHHQAPPLAQIDRIQQRSFTWL
QAPTDFSIRPSQSGAMATQIVPDAATCPQCLREMNDPQDRRYRYPFINCTHCGPRFTIIRAMPYDRPATSMAPFTLCPHC
AWEYHSAQDRRFHAQPVACSDCGPQLFSCLAQGQPQHQGDEALQQAVAALAAGQIVAIKGLGGFHLACDATNTQAVERLR
LRKRRPGKPLAVMLPDAGWLVRCVSGVSLAQLQALLTSPAAPVVLVPQSPYSPLSAAVAPGLDEVGLMLPANPIQHLLMQ
AIQRPLVMTSGNAAGRAPALTNEQALADLVDIADLWLMHDREILQRADDSLVRLLPKGHEMLRRARGFVPDALPLPPGFP
AQQALLAVGGDRKNTFCLVQGNQALLGPHFGDLEQEDIQQQWQQAIRHFTGLYHCQPQRLASDAHPGYVSHQWAARQSLP
VTPVLHHHAHLAACLAEHRWPLEGGAVIGLALDGLGYGQGGALWGGECLLVDYRRCRHLGGLPAVALPGGDLAAQQPWRN
QLAHFARFVPDWQSYPQAERLLASPWRPLLQAIKAGINAPLASSCGRLFDAAAATLGCAPAQQSWEGEAASRFEALAHQS
GPCPHPVTLSLTDEQFDLAAFWQRWLAWQAPAPQQAWAFHDALAQGFASLAKHFALQYGIGHVALSGGVLHNRLLRQRLQ
HHLAPLQILLPQQLPAGDGGLALGQALVACAQHSPASSSTFDRT

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=752, Percent_Identity=61.436170212766, Blast_Score=843, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 83251; Mature: 83120

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSGLLLRISGKVQGVGFRPFVWQLAEQLNLRGEVWNDSAGVEIRLVQPVDLEILLHVL
CCCCCEEEEECCCEECCCCCHHHHHHHHHHCCCCEECCCCCCEEEEEECCCCHHHHHHHH
HHQAPPLAQIDRIQQRSFTWLQAPTDFSIRPSQSGAMATQIVPDAATCPQCLREMNDPQD
HCCCCCHHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCHH
RRYRYPFINCTHCGPRFTIIRAMPYDRPATSMAPFTLCPHCAWEYHSAQDRRFHAQPVAC
HCEECCEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCC
SDCGPQLFSCLAQGQPQHQGDEALQQAVAALAAGQIVAIKGLGGFHLACDATNTQAVERL
CCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCHHHHHHH
RLRKRRPGKPLAVMLPDAGWLVRCVSGVSLAQLQALLTSPAAPVVLVPQSPYSPLSAAVA
HHHHCCCCCCEEEEECCCCHHHHHHCCCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHC
PGLDEVGLMLPANPIQHLLMQAIQRPLVMTSGNAAGRAPALTNEQALADLVDIADLWLMH
CCHHHCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
DREILQRADDSLVRLLPKGHEMLRRARGFVPDALPLPPGFPAQQALLAVGGDRKNTFCLV
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEE
QGNQALLGPHFGDLEQEDIQQQWQQAIRHFTGLYHCQPQRLASDAHPGYVSHQWAARQSL
ECCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHHCCC
PVTPVLHHHAHLAACLAEHRWPLEGGAVIGLALDGLGYGQGGALWGGECLLVDYRRCRHL
CCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHC
GGLPAVALPGGDLAAQQPWRNQLAHFARFVPDWQSYPQAERLLASPWRPLLQAIKAGINA
CCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCHHCCCHHHHHHHCCHHHHHHHHHHCCCC
PLASSCGRLFDAAAATLGCAPAQQSWEGEAASRFEALAHQSGPCPHPVTLSLTDEQFDLA
CHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHH
AFWQRWLAWQAPAPQQAWAFHDALAQGFASLAKHFALQYGIGHVALSGGVLHNRLLRQRL
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCHHHHHHHHHHHH
QHHLAPLQILLPQQLPAGDGGLALGQALVACAQHSPASSSTFDRT
HHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SHSGLLLRISGKVQGVGFRPFVWQLAEQLNLRGEVWNDSAGVEIRLVQPVDLEILLHVL
CCCCEEEEECCCEECCCCCHHHHHHHHHHCCCCEECCCCCCEEEEEECCCCHHHHHHHH
HHQAPPLAQIDRIQQRSFTWLQAPTDFSIRPSQSGAMATQIVPDAATCPQCLREMNDPQD
HCCCCCHHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCHH
RRYRYPFINCTHCGPRFTIIRAMPYDRPATSMAPFTLCPHCAWEYHSAQDRRFHAQPVAC
HCEECCEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCC
SDCGPQLFSCLAQGQPQHQGDEALQQAVAALAAGQIVAIKGLGGFHLACDATNTQAVERL
CCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCHHHHHHH
RLRKRRPGKPLAVMLPDAGWLVRCVSGVSLAQLQALLTSPAAPVVLVPQSPYSPLSAAVA
HHHHCCCCCCEEEEECCCCHHHHHHCCCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHC
PGLDEVGLMLPANPIQHLLMQAIQRPLVMTSGNAAGRAPALTNEQALADLVDIADLWLMH
CCHHHCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
DREILQRADDSLVRLLPKGHEMLRRARGFVPDALPLPPGFPAQQALLAVGGDRKNTFCLV
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEE
QGNQALLGPHFGDLEQEDIQQQWQQAIRHFTGLYHCQPQRLASDAHPGYVSHQWAARQSL
ECCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHHCCC
PVTPVLHHHAHLAACLAEHRWPLEGGAVIGLALDGLGYGQGGALWGGECLLVDYRRCRHL
CCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHC
GGLPAVALPGGDLAAQQPWRNQLAHFARFVPDWQSYPQAERLLASPWRPLLQAIKAGINA
CCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCHHCCCHHHHHHHCCHHHHHHHHHHCCCC
PLASSCGRLFDAAAATLGCAPAQQSWEGEAASRFEALAHQSGPCPHPVTLSLTDEQFDLA
CHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHH
AFWQRWLAWQAPAPQQAWAFHDALAQGFASLAKHFALQYGIGHVALSGGVLHNRLLRQRL
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCHHHHHHHHHHHH
QHHLAPLQILLPQQLPAGDGGLALGQALVACAQHSPASSSTFDRT
HHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8661925; 12206761 [H]