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The map label for this gene is yisS [H]

Identifier: 157370633

GI number: 157370633

Start: 2614995

End: 2616011

Strand: Direct

Name: yisS [H]

Synonym: Spro_2393

Alternate gene names: 157370633

Gene position: 2614995-2616011 (Clockwise)

Preceding gene: 157370627

Following gene: 157370634

Centisome position: 47.99

GC content: 60.87

Gene sequence:

>1017_bases
ATGAGCCATTTACCCCAAACCCTGCGTGTTGGTCTGATTGGTGCCGGACGAATGGGCAGTTTCCATGCAGAGTCTCTGGC
GCGGCGTGTGCCGGGCGCGGTACTGGCCGCAGTGGCCGATCCGTTACCAGGGGCCGCGCAGCGGCTGGCGGAACGTCTGG
GCGTTAAGGCCCATAGCGATCTGCAGGCGATGCTGGATGACCCGGCCATTGATGCCGTAGTCATCGCTTCTCCGGCGCGC
ACCCATGCGCAGTGGGTGATAGCGGCAGCCAGGGCGGGTAAACACGTCTTCTGTGAAAAACCGATGGCCGTCACGCTGGA
GGAGGCAGACTGCGCCATCGCCGCCGCCAATCAGGCGGGGGTGGTGCTACAGGTGGGGTTTAACCGCCGTTTTGTCAGCG
GTTTCGCAGCGGCCATTGCCGCCGTCAAGTCGGGCGAAAATGGCACCACCCAGTTGTCCCGCTCGGTCACCCGCGACCCG
CAGTTGCGCGATCCGGCGCCGATCCCGGCGTGGACCATCTTTCTGGAAACCCTGATCCATGATTTTGACACCTTGCTGCA
CTTCAACCCCGGAGCCAGGCCGGTAGAGATTTACGCCCTGGCGGATGCGCTGGTGCGTCCGGACTTCAAGGACAAGGGGT
TGCTGGACACCGCAGTAGTCACTATTCGCTTTGATAACGGCGCTATCGCGACGGCCGAGGCTAATTTCCAGGCGGTCTAC
GGCTATGACGTACGCGGTGAAGTGTTTGGCAGTAAAGGCATGTTGCAGGCGGGCAATATCAATTTGAATAACTGCGTGCG
TTATCACGCCGGGGGCATCGCCATCGAGACTTCGCGCCAGGATACCGATCTGCTGCATGACGCCTATGTGGCGGAGCTGA
CCGAATTCGCCCAATGCATTCAGCAAGGACAAAAGCCTCGGGCGACGGGGCAGGATGCGCGCAATGCCCTGGAAATCGCA
CTGGCCTGTATTTCGTCGGTACAGCAGGGCAAACCGATCAGGTTGGGGGAACAATAA

Upstream 100 bases:

>100_bases
AACGCCTCACAAATTCCCCGCGTTTTAAGCTGGCATCCCCTTTGCAATACCCTCAGTGAACTTGTTGCCCGCAAGGGCGT
TACCCACATGAGGAATCACG

Downstream 100 bases:

>100_bases
TGGCGAGTTTTCAACTGTCCGTTTGCGCCGAAATGGTGTTTCTCGACCTGCCGTTTGCAGAACGTGTAGCGCGTATTGAT
GCGCTGGGTTTCGGCGTCGA

Product: inositol 2-dehydrogenase

Products: 2,4,6/3,5-pentahydroxycyclohexanone; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MSHLPQTLRVGLIGAGRMGSFHAESLARRVPGAVLAAVADPLPGAAQRLAERLGVKAHSDLQAMLDDPAIDAVVIASPAR
THAQWVIAAARAGKHVFCEKPMAVTLEEADCAIAAANQAGVVLQVGFNRRFVSGFAAAIAAVKSGENGTTQLSRSVTRDP
QLRDPAPIPAWTIFLETLIHDFDTLLHFNPGARPVEIYALADALVRPDFKDKGLLDTAVVTIRFDNGAIATAEANFQAVY
GYDVRGEVFGSKGMLQAGNINLNNCVRYHAGGIAIETSRQDTDLLHDAYVAELTEFAQCIQQGQKPRATGQDARNALEIA
LACISSVQQGKPIRLGEQ

Sequences:

>Translated_338_residues
MSHLPQTLRVGLIGAGRMGSFHAESLARRVPGAVLAAVADPLPGAAQRLAERLGVKAHSDLQAMLDDPAIDAVVIASPAR
THAQWVIAAARAGKHVFCEKPMAVTLEEADCAIAAANQAGVVLQVGFNRRFVSGFAAAIAAVKSGENGTTQLSRSVTRDP
QLRDPAPIPAWTIFLETLIHDFDTLLHFNPGARPVEIYALADALVRPDFKDKGLLDTAVVTIRFDNGAIATAEANFQAVY
GYDVRGEVFGSKGMLQAGNINLNNCVRYHAGGIAIETSRQDTDLLHDAYVAELTEFAQCIQQGQKPRATGQDARNALEIA
LACISSVQQGKPIRLGEQ
>Mature_337_residues
SHLPQTLRVGLIGAGRMGSFHAESLARRVPGAVLAAVADPLPGAAQRLAERLGVKAHSDLQAMLDDPAIDAVVIASPART
HAQWVIAAARAGKHVFCEKPMAVTLEEADCAIAAANQAGVVLQVGFNRRFVSGFAAAIAAVKSGENGTTQLSRSVTRDPQ
LRDPAPIPAWTIFLETLIHDFDTLLHFNPGARPVEIYALADALVRPDFKDKGLLDTAVVTIRFDNGAIATAEANFQAVYG
YDVRGEVFGSKGMLQAGNINLNNCVRYHAGGIAIETSRQDTDLLHDAYVAELTEFAQCIQQGQKPRATGQDARNALEIAL
ACISSVQQGKPIRLGEQ

Specific function: Unknown

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Escherichia coli, GI87082405, Length=241, Percent_Identity=28.2157676348548, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1787574, Length=254, Percent_Identity=29.5275590551181, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87081947, Length=164, Percent_Identity=35.3658536585366, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789848, Length=90, Percent_Identity=38.8888888888889, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: 1.1.1.18

Molecular weight: Translated: 35990; Mature: 35859

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHLPQTLRVGLIGAGRMGSFHAESLARRVPGAVLAAVADPLPGAAQRLAERLGVKAHSD
CCCCCCHHHEEEEECCCCCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCH
LQAMLDDPAIDAVVIASPARTHAQWVIAAARAGKHVFCEKPMAVTLEEADCAIAAANQAG
HHHHHCCCCCCEEEEECCCCHHHHHHEEHHHCCCEEEECCCCEEEEECCCEEEEECCCCC
VVLQVGFNRRFVSGFAAAIAAVKSGENGTTQLSRSVTRDPQLRDPAPIPAWTIFLETLIH
EEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
DFDTLLHFNPGARPVEIYALADALVRPDFKDKGLLDTAVVTIRFDNGAIATAEANFQAVY
HHHHHEECCCCCCCEEEEEEHHHHHCCCCCCCCCEEEEEEEEEECCCEEEEECCCEEEEE
GYDVRGEVFGSKGMLQAGNINLNNCVRYHAGGIAIETSRQDTDLLHDAYVAELTEFAQCI
EECCCCEEECCCCCEEECCCCHHHHEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
QQGQKPRATGQDARNALEIALACISSVQQGKPIRLGEQ
HCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SHLPQTLRVGLIGAGRMGSFHAESLARRVPGAVLAAVADPLPGAAQRLAERLGVKAHSD
CCCCCHHHEEEEECCCCCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCH
LQAMLDDPAIDAVVIASPARTHAQWVIAAARAGKHVFCEKPMAVTLEEADCAIAAANQAG
HHHHHCCCCCCEEEEECCCCHHHHHHEEHHHCCCEEEECCCCEEEEECCCEEEEECCCCC
VVLQVGFNRRFVSGFAAAIAAVKSGENGTTQLSRSVTRDPQLRDPAPIPAWTIFLETLIH
EEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
DFDTLLHFNPGARPVEIYALADALVRPDFKDKGLLDTAVVTIRFDNGAIATAEANFQAVY
HHHHHEECCCCCCCEEEEEEHHHHHCCCCCCCCCEEEEEEEEEECCCEEEEECCCEEEEE
GYDVRGEVFGSKGMLQAGNINLNNCVRYHAGGIAIETSRQDTDLLHDAYVAELTEFAQCI
EECCCCEEECCCCCEEECCCCHHHHEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
QQGQKPRATGQDARNALEIALACISSVQQGKPIRLGEQ
HCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: myo-inositol; NAD+

Specific reaction: myo-inositol + NAD+ = 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377; 8407808 [H]