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The map label for this gene is purR

Identifier: 157370435

GI number: 157370435

Start: 2397239

End: 2398264

Strand: Reverse

Name: purR

Synonym: Spro_2195

Alternate gene names: 157370435

Gene position: 2398264-2397239 (Counterclockwise)

Preceding gene: 157370436

Following gene: 157370433

Centisome position: 44.01

GC content: 55.65

Gene sequence:

>1026_bases
ATGGCAACGATTAAAGATGTGGCCAAACGCGCTGGCGTTTCCACCACCACCGTTTCGCACGTCATCAATAAGACTCGTTT
CGTCGCCGAAGAGACAAAAGCGGCCGTTGGCGCTGCGATTAAAGAGCTGCATTACTCACCCAGCGCCGTAGCGCGCAGCC
TGAAGGTCAATCACACCAAATCGATTGGCCTGCTGGCCACCTCCAGCGAAGCCCCCTACTTTGCCGAAGTGATCGAGGCG
GTAGAAAACAGCTGCTACAGCAAAGGCTATACGCTGATTTTGTGTAACTCGCACAACAATCTGGACAAACAGCGGGCCTA
TCTGGCGATGCTGGCGCAAAAGCGTGTCGATGGCCTGCTGGTGATGTGTTCGGAATATCCAGACCAATTGCTCGGCATGC
TGGAAGACTACCGCAACATCCCAATGGTCGTGATGGACTGGGGCGCGGCCCGCGGTGATTTTACCGATAGCATCATTGAT
AACGCCTTCGCAGGCGGCTATCTGGCCGGGCGGTATCTGATTGAACGCGGTCACCGCGATATCGGAGCCATTCCAGGCCA
ACTGTCGCGCAATACCGGTGGCGGCCGCCATCAGGGCTTTTTAAAAGCCATGGAAGAAGCCAATATTGAAGTACGTGACG
AGTGGATTGTTCAGGGTGACTTTGAGCCGGAATCCGGCTACAAGGCCATGCACCAGATCCTGTCGCAAAAACATCGCCCG
ACCGCGGTATTCTGCGGCGGCGACATCATGGCGATGGGCGCGATCTGCGCCGCCGACGAACTTGGGCTGCGGGTGCCACA
AGACATTTCGGTGATTGGCTACGATAACGTGCGTAACGCCCGCTATTTCACCCCGGCACTGACCACCATTCATCAACCCA
AAGAGCGTTTGGGTGAAATGGCGTTCACCATGTTGCTGGACCGTATTATCAGCAAGCGTGAAGAGTCGCAGGTGATTGAA
GTGCATCCGAAACTGATTGAGCGTCGTTCGGTCGCTGACGGCCCATTCATCGATTACCGCCGCTAA

Upstream 100 bases:

>100_bases
AAAACTCAGTTTTCGCTTCTTTGGAGAAGGATCTACGCAGGCAAACGATTAACATCACACTTACATACTGCAACATGTGA
GTTCAGGAGTCATTCTTTAC

Downstream 100 bases:

>100_bases
TTGCTAATGCCGGAGGCCGCTGTGGTTTCCGGCACCACTTCGCTTAGCCATTCCTGATTCAGCGTTTCACTGTCCCCCAG
GTAATCCAACAGCCAGGCCA

Product: DNA-binding transcriptional repressor PurR

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MATIKDVAKRAGVSTTTVSHVINKTRFVAEETKAAVGAAIKELHYSPSAVARSLKVNHTKSIGLLATSSEAPYFAEVIEA
VENSCYSKGYTLILCNSHNNLDKQRAYLAMLAQKRVDGLLVMCSEYPDQLLGMLEDYRNIPMVVMDWGAARGDFTDSIID
NAFAGGYLAGRYLIERGHRDIGAIPGQLSRNTGGGRHQGFLKAMEEANIEVRDEWIVQGDFEPESGYKAMHQILSQKHRP
TAVFCGGDIMAMGAICAADELGLRVPQDISVIGYDNVRNARYFTPALTTIHQPKERLGEMAFTMLLDRIISKREESQVIE
VHPKLIERRSVADGPFIDYRR

Sequences:

>Translated_341_residues
MATIKDVAKRAGVSTTTVSHVINKTRFVAEETKAAVGAAIKELHYSPSAVARSLKVNHTKSIGLLATSSEAPYFAEVIEA
VENSCYSKGYTLILCNSHNNLDKQRAYLAMLAQKRVDGLLVMCSEYPDQLLGMLEDYRNIPMVVMDWGAARGDFTDSIID
NAFAGGYLAGRYLIERGHRDIGAIPGQLSRNTGGGRHQGFLKAMEEANIEVRDEWIVQGDFEPESGYKAMHQILSQKHRP
TAVFCGGDIMAMGAICAADELGLRVPQDISVIGYDNVRNARYFTPALTTIHQPKERLGEMAFTMLLDRIISKREESQVIE
VHPKLIERRSVADGPFIDYRR
>Mature_340_residues
ATIKDVAKRAGVSTTTVSHVINKTRFVAEETKAAVGAAIKELHYSPSAVARSLKVNHTKSIGLLATSSEAPYFAEVIEAV
ENSCYSKGYTLILCNSHNNLDKQRAYLAMLAQKRVDGLLVMCSEYPDQLLGMLEDYRNIPMVVMDWGAARGDFTDSIIDN
AFAGGYLAGRYLIERGHRDIGAIPGQLSRNTGGGRHQGFLKAMEEANIEVRDEWIVQGDFEPESGYKAMHQILSQKHRPT
AVFCGGDIMAMGAICAADELGLRVPQDISVIGYDNVRNARYFTPALTTIHQPKERLGEMAFTMLLDRIISKREESQVIEV
HPKLIERRSVADGPFIDYRR

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787948, Length=341, Percent_Identity=82.9912023460411, Blast_Score=601, Evalue=1e-173,
Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=48.4848484848485, Blast_Score=320, Evalue=1e-88,
Organism=Escherichia coli, GI1790369, Length=309, Percent_Identity=34.9514563106796, Blast_Score=201, Evalue=5e-53,
Organism=Escherichia coli, GI1787580, Length=326, Percent_Identity=32.8220858895706, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1789202, Length=293, Percent_Identity=35.4948805460751, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1788474, Length=332, Percent_Identity=30.1204819277108, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1789068, Length=316, Percent_Identity=29.4303797468354, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI1786540, Length=309, Percent_Identity=32.6860841423948, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI48994940, Length=325, Percent_Identity=28.3076923076923, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789456, Length=334, Percent_Identity=29.3413173652695, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1790715, Length=327, Percent_Identity=25.0764525993884, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787906, Length=258, Percent_Identity=26.7441860465116, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786268, Length=279, Percent_Identity=26.5232974910394, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790689, Length=320, Percent_Identity=23.125, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790192, Length=245, Percent_Identity=25.7142857142857, Blast_Score=71, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURR_SERP5 (A8GDV6)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001478424.1
- ProteinModelPortal:   A8GDV6
- SMR:   A8GDV6
- GeneID:   5605580
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_2195
- eggNOG:   COG1609
- HOGENOM:   HBG753640
- OMA:   ICAADEM
- ProtClustDB:   PRK10703
- BioCyc:   SPRO399741:SPRO_2195-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01277
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761
- PRINTS:   PR00036
- SMART:   SM00354

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1; SSF47413 Lambda_like_DNA

EC number: NA

Molecular weight: Translated: 37784; Mature: 37653

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00356 HTH_LACI_1; PS50932 HTH_LACI_2

Important sites: BINDING 73-73 BINDING 190-190 BINDING 192-192 BINDING 221-221 BINDING 275-275

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVAKRAGVSTTTVSHVINKTRFVAEETKAAVGAAIKELHYSPSAVARSLKVNHTK
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
SIGLLATSSEAPYFAEVIEAVENSCYSKGYTLILCNSHNNLDKQRAYLAMLAQKRVDGLL
CEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCEE
VMCSEYPDQLLGMLEDYRNIPMVVMDWGAARGDFTDSIIDNAFAGGYLAGRYLIERGHRD
EEHHHCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCC
IGAIPGQLSRNTGGGRHQGFLKAMEEANIEVRDEWIVQGDFEPESGYKAMHQILSQKHRP
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCC
TAVFCGGDIMAMGAICAADELGLRVPQDISVIGYDNVRNARYFTPALTTIHQPKERLGEM
CEEEECCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEECHHHHHHHCHHHHHHHH
AFTMLLDRIISKREESQVIEVHPKLIERRSVADGPFIDYRR
HHHHHHHHHHHHHHHCCHHHHCHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
ATIKDVAKRAGVSTTTVSHVINKTRFVAEETKAAVGAAIKELHYSPSAVARSLKVNHTK
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
SIGLLATSSEAPYFAEVIEAVENSCYSKGYTLILCNSHNNLDKQRAYLAMLAQKRVDGLL
CEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCEE
VMCSEYPDQLLGMLEDYRNIPMVVMDWGAARGDFTDSIIDNAFAGGYLAGRYLIERGHRD
EEHHHCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCC
IGAIPGQLSRNTGGGRHQGFLKAMEEANIEVRDEWIVQGDFEPESGYKAMHQILSQKHRP
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCC
TAVFCGGDIMAMGAICAADELGLRVPQDISVIGYDNVRNARYFTPALTTIHQPKERLGEM
CEEEECCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEECHHHHHHHCHHHHHHHH
AFTMLLDRIISKREESQVIEVHPKLIERRSVADGPFIDYRR
HHHHHHHHHHHHHHHCCHHHHCHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA