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The map label for this gene is lplA

Identifier: 157370402

GI number: 157370402

Start: 2358250

End: 2359263

Strand: Direct

Name: lplA

Synonym: Spro_2162

Alternate gene names: 157370402

Gene position: 2358250-2359263 (Clockwise)

Preceding gene: 157370401

Following gene: 157370403

Centisome position: 43.28

GC content: 53.85

Gene sequence:

>1014_bases
ATGAGTAACTTACGCCTGTTGATTTCCGATTCCTACGATCCCTGGTTCAATCTGGCAGTGGAAGAGTGCATCTTCCGCGA
AATGACTACGCAGAAAATTCTGTTCCTGTGGCGCAATGCCGAAACCGTGGTCATTGGTCAGTCACAAAACCCCTGGAAAG
AGTGCAACACCCGCCGCATGGAGCAGGATGGTATTCGTCTGGCACGCCGCAGCAGCGGCGGTGGTGCGGTATTCCACGAT
TTGGGCAATAGCTGTTTTACCTTTATGGCGGGCAAACCCGGTTACGATAAAACCGTCTCTACCGGCATTATTCTGCAAGC
TCTGGCTCAGTTGGGCATTACGGCCACCGCATCGGGACGCAATGACCTGGTGATTGATACCGCAGAGGGCGTGCGTAAAA
TCTCCGGCTCGGCCTACCGGGAAACTCAGGATCGCGGGTTCCACCATGGCACCTTGTTGCTGAACGCGGATCTCAATCGC
CTGGCCGACTACCTTAATCCGGACCCTAAAAAATTGCAGGCCAAGGGCATCACCTCGGTACGTTCTCGCGTTGCCAATCT
GGCGGAATTTAAAGCCGATATCAGCCACCACCAGGTGTGCGACGCAATCACTCAGGCGTTCTTTGACCATTATGGTGAGA
TGGCGAAAGCAGAAATCATTTCGCCTGACGTTTATCCCGATTTGCCGGATTTTGCCGCGCAGTTTGCCAAACAAAGCAGT
TGGCAATGGAATTTCGGCAAGGCGCCGGCATTCAGTCACCTGCTGAATGAACGGTTTGTCTGGGGTGGGGTAGATATCTT
CTTTGACGTGGAAAAAGGCGCCATTTGCCGGGCGCAGATTTTTACCGACAGTCTCAGCCCGGCACCGTTGCAACGGCTGG
CCGAGATGCTGGTGGGCTGCACCTATCGCAGCGAGGCGTTGGCGAATTGCTGCGATGCTTTGATTGTGCAGTACCCAGAG
CAGGCCGCAGAATTAACCGAGCTGCGACAATGGTTGCTTGAAACGATCAAATAA

Upstream 100 bases:

>100_bases
CGTCTACTGGAAGAAGGTCTACTGGCAGGCGCGTCGCATTTAGCTCTAACCTCTTGATTTGGTGTATCATGGCGGTATCA
CATTGTTAACGGTGGCCGCC

Downstream 100 bases:

>100_bases
CGTTCGGATCCTGAATGCTCCGGGATGAGGCAACTCATCCCTTTTTTACGCCGCGAGTTTGCCTCTCTTTCATGCCGTAA
TCTTTTTTTGCAAAAAAATG

Product: lipoate-protein ligase A

Products: NA

Alternate protein names: Lipoate--protein ligase

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MSNLRLLISDSYDPWFNLAVEECIFREMTTQKILFLWRNAETVVIGQSQNPWKECNTRRMEQDGIRLARRSSGGGAVFHD
LGNSCFTFMAGKPGYDKTVSTGIILQALAQLGITATASGRNDLVIDTAEGVRKISGSAYRETQDRGFHHGTLLLNADLNR
LADYLNPDPKKLQAKGITSVRSRVANLAEFKADISHHQVCDAITQAFFDHYGEMAKAEIISPDVYPDLPDFAAQFAKQSS
WQWNFGKAPAFSHLLNERFVWGGVDIFFDVEKGAICRAQIFTDSLSPAPLQRLAEMLVGCTYRSEALANCCDALIVQYPE
QAAELTELRQWLLETIK

Sequences:

>Translated_337_residues
MSNLRLLISDSYDPWFNLAVEECIFREMTTQKILFLWRNAETVVIGQSQNPWKECNTRRMEQDGIRLARRSSGGGAVFHD
LGNSCFTFMAGKPGYDKTVSTGIILQALAQLGITATASGRNDLVIDTAEGVRKISGSAYRETQDRGFHHGTLLLNADLNR
LADYLNPDPKKLQAKGITSVRSRVANLAEFKADISHHQVCDAITQAFFDHYGEMAKAEIISPDVYPDLPDFAAQFAKQSS
WQWNFGKAPAFSHLLNERFVWGGVDIFFDVEKGAICRAQIFTDSLSPAPLQRLAEMLVGCTYRSEALANCCDALIVQYPE
QAAELTELRQWLLETIK
>Mature_336_residues
SNLRLLISDSYDPWFNLAVEECIFREMTTQKILFLWRNAETVVIGQSQNPWKECNTRRMEQDGIRLARRSSGGGAVFHDL
GNSCFTFMAGKPGYDKTVSTGIILQALAQLGITATASGRNDLVIDTAEGVRKISGSAYRETQDRGFHHGTLLLNADLNRL
ADYLNPDPKKLQAKGITSVRSRVANLAEFKADISHHQVCDAITQAFFDHYGEMAKAEIISPDVYPDLPDFAAQFAKQSSW
QWNFGKAPAFSHLLNERFVWGGVDIFFDVEKGAICRAQIFTDSLSPAPLQRLAEMLVGCTYRSEALANCCDALIVQYPEQ
AAELTELRQWLLETIK

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family

Homologues:

Organism=Homo sapiens, GI7706252, Length=284, Percent_Identity=33.8028169014084, Blast_Score=158, Evalue=6e-39,
Organism=Homo sapiens, GI21729884, Length=284, Percent_Identity=33.8028169014084, Blast_Score=158, Evalue=6e-39,
Organism=Homo sapiens, GI21729882, Length=284, Percent_Identity=33.8028169014084, Blast_Score=158, Evalue=6e-39,
Organism=Homo sapiens, GI21729880, Length=284, Percent_Identity=33.8028169014084, Blast_Score=158, Evalue=6e-39,
Organism=Homo sapiens, GI21729878, Length=284, Percent_Identity=33.8028169014084, Blast_Score=158, Evalue=6e-39,
Organism=Escherichia coli, GI1790846, Length=338, Percent_Identity=72.7810650887574, Blast_Score=531, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI71981909, Length=243, Percent_Identity=32.0987654320988, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6322415, Length=277, Percent_Identity=28.5198555956679, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28573464, Length=336, Percent_Identity=29.7619047619048, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LPLA_SERP5 (A8GDS3)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001478391.1
- ProteinModelPortal:   A8GDS3
- SMR:   A8GDS3
- STRING:   A8GDS3
- GeneID:   5605881
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_2162
- eggNOG:   COG0095
- HOGENOM:   HBG715377
- OMA:   YEQSHLE
- ProtClustDB:   PRK03822
- BioCyc:   SPRO399741:SPRO_2162-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01602
- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562
- Gene3D:   G3DSA:3.30.390.50
- TIGRFAMs:   TIGR00545

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C

EC number: =2.7.7.63

Molecular weight: Translated: 37681; Mature: 37550

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: BINDING 70-70 BINDING 133-133 BINDING 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLRLLISDSYDPWFNLAVEECIFREMTTQKILFLWRNAETVVIGQSQNPWKECNTRRM
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEECCCCCHHHHHHHHH
EQDGIRLARRSSGGGAVFHDLGNSCFTFMAGKPGYDKTVSTGIILQALAQLGITATASGR
HHHHHHHHHCCCCCCCHHHHCCCHHEEEECCCCCCCCHHHHHHHHHHHHHHCCEEECCCC
NDLVIDTAEGVRKISGSAYRETQDRGFHHGTLLLNADLNRLADYLNPDPKKLQAKGITSV
CCEEEECHHHHHHHCCCHHHHHHHCCCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHH
RSRVANLAEFKADISHHQVCDAITQAFFDHYGEMAKAEIISPDVYPDLPDFAAQFAKQSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCC
WQWNFGKAPAFSHLLNERFVWGGVDIFFDVEKGAICRAQIFTDSLSPAPLQRLAEMLVGC
CCCCCCCCHHHHHHHHCCCEECCEEEEEECCCCCEEEEEHHHCCCCHHHHHHHHHHHHCC
TYRSEALANCCDALIVQYPEQAAELTELRQWLLETIK
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNLRLLISDSYDPWFNLAVEECIFREMTTQKILFLWRNAETVVIGQSQNPWKECNTRRM
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEECCCCCHHHHHHHHH
EQDGIRLARRSSGGGAVFHDLGNSCFTFMAGKPGYDKTVSTGIILQALAQLGITATASGR
HHHHHHHHHCCCCCCCHHHHCCCHHEEEECCCCCCCCHHHHHHHHHHHHHHCCEEECCCC
NDLVIDTAEGVRKISGSAYRETQDRGFHHGTLLLNADLNRLADYLNPDPKKLQAKGITSV
CCEEEECHHHHHHHCCCHHHHHHHCCCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHH
RSRVANLAEFKADISHHQVCDAITQAFFDHYGEMAKAEIISPDVYPDLPDFAAQFAKQSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCC
WQWNFGKAPAFSHLLNERFVWGGVDIFFDVEKGAICRAQIFTDSLSPAPLQRLAEMLVGC
CCCCCCCCHHHHHHHHCCCEECCEEEEEECCCCCEEEEEHHHCCCCHHHHHHHHHHHHCC
TYRSEALANCCDALIVQYPEQAAELTELRQWLLETIK
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA