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The map label for this gene is tolA [H]

Identifier: 157370291

GI number: 157370291

Start: 2243251

End: 2243691

Strand: Direct

Name: tolA [H]

Synonym: Spro_2049

Alternate gene names: 157370291

Gene position: 2243251-2243691 (Clockwise)

Preceding gene: 157370290

Following gene: 157370295

Centisome position: 41.17

GC content: 51.25

Gene sequence:

>441_bases
ATGAATAGAGTTCATTTAGGGTTTAAAAAAAACCTGCCGGCATTAATGCTTATTGTCGTCTCACTTTGGCTGACAGGCTG
TACCAAGACGAACGCTGTGGCTAACGCTGGGCCGTCTGCGGAGGGTGTCGACGATCTTTTTGCTGCCCTTGACGGCAAAG
CAACTCCTGCAGCAGGGGATGAGGTCAGCCACTATGCCGGGCAGGTACGGGCGGCGATTCAAAGTCATTTTTATGATGCC
AACGCTTATGCCGGCAAGCAATGTACTATCAGAATGAAGTTTGCCCCGGATGGGATGCTTTTGGCTGTCCGCTCTGAAAG
TGGTGATCCTGCGCTTTGCCGGGCTGCTATAAAGGCAATCACAAATACCCGTTTCCCCAAGCCGCCGTCACAAGCGGTTT
ACGGTGCTTTTAAAGATTCCGTACTGGAATTTCGCCCCTAA

Upstream 100 bases:

>100_bases
AAACACATGTAATGGTGTTTTGCGTTTGAATAAGAACGGGAGCCTGATGGCTCCCGTTTTTTATTGTTCTTTCCAGTGAC
TATTCTAAAAGAAGGGAATC

Downstream 100 bases:

>100_bases
GCGGCTGAATGACATAACTATCGTTAAACGTCTGTAATTGTGTTTAAGCATTTGAACAACAACGGGAGCCGTAGGGCTCC
CGTTGTTTACTGCCGTTTTA

Product: colicin uptake-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MNRVHLGFKKNLPALMLIVVSLWLTGCTKTNAVANAGPSAEGVDDLFAALDGKATPAAGDEVSHYAGQVRAAIQSHFYDA
NAYAGKQCTIRMKFAPDGMLLAVRSESGDPALCRAAIKAITNTRFPKPPSQAVYGAFKDSVLEFRP

Sequences:

>Translated_146_residues
MNRVHLGFKKNLPALMLIVVSLWLTGCTKTNAVANAGPSAEGVDDLFAALDGKATPAAGDEVSHYAGQVRAAIQSHFYDA
NAYAGKQCTIRMKFAPDGMLLAVRSESGDPALCRAAIKAITNTRFPKPPSQAVYGAFKDSVLEFRP
>Mature_146_residues
MNRVHLGFKKNLPALMLIVVSLWLTGCTKTNAVANAGPSAEGVDDLFAALDGKATPAAGDEVSHYAGQVRAAIQSHFYDA
NAYAGKQCTIRMKFAPDGMLLAVRSESGDPALCRAAIKAITNTRFPKPPSQAVYGAFKDSVLEFRP

Specific function: Involved in the tonB-independent uptake of group A colicins (colicins A, E1, E2, E3, and K). Necessary for the colicins to reach their respective targets after initial binding to the bacteria. Also involved in the translocation of bacteriophage DNA [H]

COG id: COG3064

COG function: function code M; Membrane protein involved in colicin uptake

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786960, Length=112, Percent_Identity=50.8928571428571, Blast_Score=117, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014161 [H]

Pfam domain/function: PF06519 TolA [H]

EC number: NA

Molecular weight: Translated: 15541; Mature: 15541

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRVHLGFKKNLPALMLIVVSLWLTGCTKTNAVANAGPSAEGVDDLFAALDGKATPAAGD
CCCCCCCHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCC
EVSHYAGQVRAAIQSHFYDANAYAGKQCTIRMKFAPDGMLLAVRSESGDPALCRAAIKAI
HHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHH
TNTRFPKPPSQAVYGAFKDSVLEFRP
HCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNRVHLGFKKNLPALMLIVVSLWLTGCTKTNAVANAGPSAEGVDDLFAALDGKATPAAGD
CCCCCCCHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCC
EVSHYAGQVRAAIQSHFYDANAYAGKQCTIRMKFAPDGMLLAVRSESGDPALCRAAIKAI
HHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHH
TNTRFPKPPSQAVYGAFKDSVLEFRP
HCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2687247; 8905232; 9278503; 2068069; 8978668; 10404600 [H]