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The map label for this gene is hemK [H]

Identifier: 157370233

GI number: 157370233

Start: 2179431

End: 2180261

Strand: Direct

Name: hemK [H]

Synonym: Spro_1991

Alternate gene names: 157370233

Gene position: 2179431-2180261 (Clockwise)

Preceding gene: 157370232

Following gene: 157370234

Centisome position: 40.0

GC content: 60.77

Gene sequence:

>831_bases
ATGGATTATCAATCCTGGCTGAAAGCCGCAGCTGCCCGGTTGACGCAGAGTGACAGTGCCCGGCGTGACGCGGAAATCCT
GCTGGGTTTCGTGACCGGCCGGGCCAGAACCTTTCTGATGGCATTTGGCGAAACTTTGCTGACGCAACAACAGCAAGAAC
AGTTGGAAAGGCTGCTGGTGCGCCGCGAACGTGGCGAGCCGGTGGCTTATCTGATTGGAGAACGCGAGTTTTGGTCGCTG
CCGCTATCGGTCTCGCCGGCCACCCTGATCCCACGTCCGGATACCGAATGCCTGGTGGAACTGGCGCTGGAGCGCCTGCC
GTCATCCTCTTGCAACATCCTCGATCTTGGCACCGGCACCGGTGCCATTGCGTTGGCGCTGGCCAGCGAACGCCAGGACT
GCAGGGTGACCGGCGTTGATTTACAGCCTGAGGCCGTAGCGCTGGCGCAGCATAATGCGCAGAAGCTGGCGATCGGCAAC
GCACAATTTCTTCAGGGCAGTTGGTTTGCGCCTCTGGCCGGGCAAACTTTCGCCCTGATCGCCAGTAATCCTCCGTATAT
TGACGCCGCCGATCCGCACCTGGCGCAGGGCGACGTACGTTTCGAACCCAGCAGCGCACTGGTCGCGCAGCAGCACGGGT
TGGCGGATTTATCCGCTATTGTGCAACAGGCACCGCAGTATCTGCAGCCACAGGGCTGGCTGTTGCTGGAGCATGGTTGG
CAACAGGGCGAGAGCGTGCGCGCATTGCTGCAGGCCGCCGGTTTTATTTCGATCGCTACCCGCCGTGACTACGGTGATAA
CGATCGCGTGACTTTTGGCCAATGGCCGTAG

Upstream 100 bases:

>100_bases
TGGACGAGGTGATGGAGGGCAAGCTGGACATGCTGATCCAACCTATTGTGCAGGAATATCAGGCCGACCAGCTCGCCGCG
CTGTCCGCTGAGCCAGAGTA

Downstream 100 bases:

>100_bases
CAGTAACTGAGAACTAACAAAAACAAAGCACACGGCGAATAAACAAAAGGAAGCAATATGGCAGTCTACTCAGCGTTAAA
ACATCTGCACCTGTTGACAG

Product: N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase

Products: NA

Alternate protein names: M.EcoKHemKP; Protein-(glutamine-N(5)) MTase hemK; Protein-glutamine N-methyltransferase hemK [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MDYQSWLKAAAARLTQSDSARRDAEILLGFVTGRARTFLMAFGETLLTQQQQEQLERLLVRRERGEPVAYLIGEREFWSL
PLSVSPATLIPRPDTECLVELALERLPSSSCNILDLGTGTGAIALALASERQDCRVTGVDLQPEAVALAQHNAQKLAIGN
AQFLQGSWFAPLAGQTFALIASNPPYIDAADPHLAQGDVRFEPSSALVAQQHGLADLSAIVQQAPQYLQPQGWLLLEHGW
QQGESVRALLQAAGFISIATRRDYGDNDRVTFGQWP

Sequences:

>Translated_276_residues
MDYQSWLKAAAARLTQSDSARRDAEILLGFVTGRARTFLMAFGETLLTQQQQEQLERLLVRRERGEPVAYLIGEREFWSL
PLSVSPATLIPRPDTECLVELALERLPSSSCNILDLGTGTGAIALALASERQDCRVTGVDLQPEAVALAQHNAQKLAIGN
AQFLQGSWFAPLAGQTFALIASNPPYIDAADPHLAQGDVRFEPSSALVAQQHGLADLSAIVQQAPQYLQPQGWLLLEHGW
QQGESVRALLQAAGFISIATRRDYGDNDRVTFGQWP
>Mature_276_residues
MDYQSWLKAAAARLTQSDSARRDAEILLGFVTGRARTFLMAFGETLLTQQQQEQLERLLVRRERGEPVAYLIGEREFWSL
PLSVSPATLIPRPDTECLVELALERLPSSSCNILDLGTGTGAIALALASERQDCRVTGVDLQPEAVALAQHNAQKLAIGN
AQFLQGSWFAPLAGQTFALIASNPPYIDAADPHLAQGDVRFEPSSALVAQQHGLADLSAIVQQAPQYLQPQGWLLLEHGW
QQGESVRALLQAAGFISIATRRDYGDNDRVTFGQWP

Specific function: Methylates the translation termination release factor RF1/PrfA on 'Gln-235' and RF2/PrfB on 'Gln-252' [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=272, Percent_Identity=32.7205882352941, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1787463, Length=275, Percent_Identity=64.7272727272727, Blast_Score=359, Evalue=1e-101,
Organism=Escherichia coli, GI87082085, Length=196, Percent_Identity=32.1428571428571, Blast_Score=91, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6324266, Length=235, Percent_Identity=25.9574468085106, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24582226, Length=220, Percent_Identity=27.2727272727273, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30202; Mature: 30202

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYQSWLKAAAARLTQSDSARRDAEILLGFVTGRARTFLMAFGETLLTQQQQEQLERLLV
CCHHHHHHHHHHHHHCCCCCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRERGEPVAYLIGEREFWSLPLSVSPATLIPRPDTECLVELALERLPSSSCNILDLGTGT
HHCCCCCEEEEECCCCCEECCCCCCCCEECCCCCHHHHHHHHHHHCCCCCCCEEEECCCC
GAIALALASERQDCRVTGVDLQPEAVALAQHNAQKLAIGNAQFLQGSWFAPLAGQTFALI
CEEEEEECCCCCCCEEECCCCCCHHHHHHHCCCCEEEECCCCEECCCCCCCCCCCEEEEE
ASNPPYIDAADPHLAQGDVRFEPSSALVAQQHGLADLSAIVQQAPQYLQPQGWLLLEHGW
ECCCCCCCCCCCCCCCCCEEECCCHHHHHHHCCHHHHHHHHHHCHHHHCCCCCEEEECCC
QQGESVRALLQAAGFISIATRRDYGDNDRVTFGQWP
CCCHHHHHHHHHCCCEEEEEECCCCCCCCEECCCCC
>Mature Secondary Structure
MDYQSWLKAAAARLTQSDSARRDAEILLGFVTGRARTFLMAFGETLLTQQQQEQLERLLV
CCHHHHHHHHHHHHHCCCCCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRERGEPVAYLIGEREFWSLPLSVSPATLIPRPDTECLVELALERLPSSSCNILDLGTGT
HHCCCCCEEEEECCCCCEECCCCCCCCEECCCCCHHHHHHHHHHHCCCCCCCEEEECCCC
GAIALALASERQDCRVTGVDLQPEAVALAQHNAQKLAIGNAQFLQGSWFAPLAGQTFALI
CEEEEEECCCCCCCEEECCCCCCHHHHHHHCCCCEEEECCCCEECCCCCCCCCCCEEEEE
ASNPPYIDAADPHLAQGDVRFEPSSALVAQQHGLADLSAIVQQAPQYLQPQGWLLLEHGW
ECCCCCCCCCCCCCCCCCEEECCCHHHHHHHCCHHHHHHHHHHCHHHHCCCCCEEEECCC
QQGESVRALLQAAGFISIATRRDYGDNDRVTFGQWP
CCCHHHHHHHHHCCCEEEEEECCCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7883187; 7543480; 11805295; 8905232; 9278503 [H]