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The map label for this gene is prfA

Identifier: 157370232

GI number: 157370232

Start: 2178346

End: 2179431

Strand: Direct

Name: prfA

Synonym: Spro_1990

Alternate gene names: 157370232

Gene position: 2178346-2179431 (Clockwise)

Preceding gene: 157370231

Following gene: 157370233

Centisome position: 39.98

GC content: 59.02

Gene sequence:

>1086_bases
ATGAAGCCTTCTATTGTTGCCAAACTGGAAGCGTTACAAGAGCGCCATGAAGAAGTGCAGGCGCTGCTCGGCGATGCCGG
CGTTATTGCCGAGCAGGATCGGTTCCGCGCGCTGTCGCGCGAATATGCACAGTTGACCGATGTCAGCCGCTGTTTCCTTG
AATGGCGTAAGGTGCAAGACGATCTGGCCACGGCAGAAATGATGCTGGATGACCCGGAAATGCGCGAAATGGCGCAGGAA
GAGTTGAAAGACGCCAAGGCGGCAACGGAAGAGCTGGAGCAGCAATTACAAGTGCTGCTGTTGCCGAAAGATCCGGATGA
TGAGCGCGATTGCTTCCTGGAAGTGCGTGCCGGTACCGGCGGTGACGAAGCGGCGATTTTCGCCGGCGATCTGTTCCGTA
TGTACAGCCGCTATGCCGAAACGCGTCGCTGGCGCGTCGAGATCATGAGTGCCAACGAAGGCGAGCACGGCGGCTATAAA
GAAGTGATTGCCAAGGTATCTGGCGATGGCGTTTTCGGCCAACTGAAGTTTGAGTCTGGTGGCCACCGCGTGCAGCGCGT
GCCGGAAACCGAGTCGCAGGGGCGTATTCATACCTCCGCCTGTACCGTGGCGGTGATGCCGGCGGTGCCGGAAGCCGAGC
TGCCGGAGATCAATGCCGGCGATCTGAAAATCGATACCTTCCGTTCTTCCGGTGCCGGTGGCCAGCACGTTAACACCACC
GACTCGGCTATCCGTATTACCCACTTGCCAACCGGTATTGTGGTGGAGTGTCAGGACGAACGTTCACAGCACAAAAACAA
AGCCAAGGCGATGTCAGTGCTGGGGGCGCGTATCCGCGCTGCCGAAATGGCCAAGCGCCAGCAGGCCGAGGCATCAACCC
GTCGTAATCTGTTGGGTACCGGCGACCGCTCCGATCGCAACCGCACCTACAACTTCCCGCAGGGGCGCGTGACCGACCAC
CGCATCAACCTGACATTGTACCGTCTGGACGAGGTGATGGAGGGCAAGCTGGACATGCTGATCCAACCTATTGTGCAGGA
ATATCAGGCCGACCAGCTCGCCGCGCTGTCCGCTGAGCCAGAGTAA

Upstream 100 bases:

>100_bases
CGCCGGCGATGGCGACGTGGAGCGGTTGCAATTATTACGCGACAGCCTCGGGCTGGATCAGCATTAGTTTTCTCTTTATT
AACAGGGTTTAATACCACGC

Downstream 100 bases:

>100_bases
TGGATTATCAATCCTGGCTGAAAGCCGCAGCTGCCCGGTTGACGCAGAGTGACAGTGCCCGGCGTGACGCGGAAATCCTG
CTGGGTTTCGTGACCGGCCG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKPSIVAKLEALQERHEEVQALLGDAGVIAEQDRFRALSREYAQLTDVSRCFLEWRKVQDDLATAEMMLDDPEMREMAQE
ELKDAKAATEELEQQLQVLLLPKDPDDERDCFLEVRAGTGGDEAAIFAGDLFRMYSRYAETRRWRVEIMSANEGEHGGYK
EVIAKVSGDGVFGQLKFESGGHRVQRVPETESQGRIHTSACTVAVMPAVPEAELPEINAGDLKIDTFRSSGAGGQHVNTT
DSAIRITHLPTGIVVECQDERSQHKNKAKAMSVLGARIRAAEMAKRQQAEASTRRNLLGTGDRSDRNRTYNFPQGRVTDH
RINLTLYRLDEVMEGKLDMLIQPIVQEYQADQLAALSAEPE

Sequences:

>Translated_361_residues
MKPSIVAKLEALQERHEEVQALLGDAGVIAEQDRFRALSREYAQLTDVSRCFLEWRKVQDDLATAEMMLDDPEMREMAQE
ELKDAKAATEELEQQLQVLLLPKDPDDERDCFLEVRAGTGGDEAAIFAGDLFRMYSRYAETRRWRVEIMSANEGEHGGYK
EVIAKVSGDGVFGQLKFESGGHRVQRVPETESQGRIHTSACTVAVMPAVPEAELPEINAGDLKIDTFRSSGAGGQHVNTT
DSAIRITHLPTGIVVECQDERSQHKNKAKAMSVLGARIRAAEMAKRQQAEASTRRNLLGTGDRSDRNRTYNFPQGRVTDH
RINLTLYRLDEVMEGKLDMLIQPIVQEYQADQLAALSAEPE
>Mature_361_residues
MKPSIVAKLEALQERHEEVQALLGDAGVIAEQDRFRALSREYAQLTDVSRCFLEWRKVQDDLATAEMMLDDPEMREMAQE
ELKDAKAATEELEQQLQVLLLPKDPDDERDCFLEVRAGTGGDEAAIFAGDLFRMYSRYAETRRWRVEIMSANEGEHGGYK
EVIAKVSGDGVFGQLKFESGGHRVQRVPETESQGRIHTSACTVAVMPAVPEAELPEINAGDLKIDTFRSSGAGGQHVNTT
DSAIRITHLPTGIVVECQDERSQHKNKAKAMSVLGARIRAAEMAKRQQAEASTRRNLLGTGDRSDRNRTYNFPQGRVTDH
RINLTLYRLDEVMEGKLDMLIQPIVQEYQADQLAALSAEPE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=292, Percent_Identity=46.9178082191781, Blast_Score=265, Evalue=5e-71,
Organism=Homo sapiens, GI34577120, Length=344, Percent_Identity=39.5348837209302, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI166795305, Length=193, Percent_Identity=47.1502590673575, Blast_Score=181, Evalue=6e-46,
Organism=Escherichia coli, GI1787462, Length=357, Percent_Identity=87.6750700280112, Blast_Score=638, Evalue=0.0,
Organism=Escherichia coli, GI2367172, Length=336, Percent_Identity=39.5833333333333, Blast_Score=210, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI17542784, Length=345, Percent_Identity=32.463768115942, Blast_Score=180, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6321295, Length=313, Percent_Identity=42.4920127795527, Blast_Score=256, Evalue=5e-69,
Organism=Drosophila melanogaster, GI19921226, Length=330, Percent_Identity=38.7878787878788, Blast_Score=204, Evalue=5e-53,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_SERP5 (A8GDA3)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001478221.1
- ProteinModelPortal:   A8GDA3
- SMR:   A8GDA3
- STRING:   A8GDA3
- GeneID:   5603231
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1990
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- BioCyc:   SPRO399741:SPRO_1990-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40371; Mature: 40371

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSIVAKLEALQERHEEVQALLGDAGVIAEQDRFRALSREYAQLTDVSRCFLEWRKVQD
CCCHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLATAEMMLDDPEMREMAQEELKDAKAATEELEQQLQVLLLPKDPDDERDCFLEVRAGTG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEEECCCC
GDEAAIFAGDLFRMYSRYAETRRWRVEIMSANEGEHGGYKEVIAKVSGDGVFGQLKFESG
CCCCEEHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEECCC
GHRVQRVPETESQGRIHTSACTVAVMPAVPEAELPEINAGDLKIDTFRSSGAGGQHVNTT
CCEEEECCCCCCCCEEEECEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCC
DSAIRITHLPTGIVVECQDERSQHKNKAKAMSVLGARIRAAEMAKRQQAEASTRRNLLGT
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVMEGKLDMLIQPIVQEYQADQLAALSAEP
CCCCCCCCCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCC
E
C
>Mature Secondary Structure
MKPSIVAKLEALQERHEEVQALLGDAGVIAEQDRFRALSREYAQLTDVSRCFLEWRKVQD
CCCHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLATAEMMLDDPEMREMAQEELKDAKAATEELEQQLQVLLLPKDPDDERDCFLEVRAGTG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEEECCCC
GDEAAIFAGDLFRMYSRYAETRRWRVEIMSANEGEHGGYKEVIAKVSGDGVFGQLKFESG
CCCCEEHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEECCC
GHRVQRVPETESQGRIHTSACTVAVMPAVPEAELPEINAGDLKIDTFRSSGAGGQHVNTT
CCEEEECCCCCCCCEEEECEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCC
DSAIRITHLPTGIVVECQDERSQHKNKAKAMSVLGARIRAAEMAKRQQAEASTRRNLLGT
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVMEGKLDMLIQPIVQEYQADQLAALSAEP
CCCCCCCCCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA