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The map label for this gene is ghrA

Identifier: 157370175

GI number: 157370175

Start: 2118153

End: 2119094

Strand: Direct

Name: ghrA

Synonym: Spro_1933

Alternate gene names: 157370175

Gene position: 2118153-2119094 (Clockwise)

Preceding gene: 157370174

Following gene: 157370176

Centisome position: 38.87

GC content: 59.55

Gene sequence:

>942_bases
ATGAACATCATTTTTTACCACCCTTTCTTTAATGCCGAGGAATGGCTGGCAGGCCTGTCACAGCGCCTGCCGCAAGCGCA
GGTTCGTGCCTGGCAACCGGGCGATGATCGTCCGGCAGATTACGCGTTGGTGTGGCGGCCACCCCATGAGATGTTGGCTA
ACCGTGGTCGGCTGAAAGGCGTGTTTGCCCTCGGTGCCGGCGTCGATGCCATCCTGGAGCAAGAACGTGCTTACCCCGGT
ACCCTGCCGGCAGGTGTGCCGTTGCTGCGCCTTGAAGATACCGGCATGGCGCAACAGATGCAGGAATATGCCCTGAGCTA
CGTCCTGCGCTATTTCCGCCGTTTTGATGAATACCAACTGTTGCAACAGCAGCAAGAGTGGCGGCCGCTCGATCCCCATC
AGCAGGATAACTTCACTATTGGCATTCTCGGTGCCGGGGTGTTGGGCAAAAGCGTGGCGCAGAAGCTGACGGCGTTTGGT
TTTAACGTGCGTTGCTGGAGCCGTAGCCCCAAACAAATCGACGGCGTGCAGAGCTTTGCCGGAGCCGAACAGCGCGCCGC
GTTTCTCGATGGTGTGCAATTGCTGATCAATCTGCTGCCCAATACGCCGGAAACCGCCGGGATCCTCAATCAGCAACTGT
TCGCGCATTTGGCGCCGGGGGCTTATCTGATCAACCTGGCACGGGGTGCGCATCTGGTTGAGGACGATCTGTTGCAGGCG
CTGGAGCAAGGCCAACTTGCCGCCGCCACGCTGGATGTGTTCGTCACCGAGCCGCTGCCGCAGGCGCATCCGTTCTGGCG
TCATCCGCGTGTCACCATTACCCCGCATATTGCTGCCATCACCTTGCCGCAAGCGGCGATGGATCAGATAGCGGCGAACA
TTCTCGCGCTTGAGGCCGGACAAACACCGGCCGGAGTGGTAGATGTGCTGCTCGGTTACTGA

Upstream 100 bases:

>100_bases
CTGGTCGGGGCTGTTTTCGTTTTGGGCTTTAACGCTTGCATCTGCCGACGAATCGCGGAAGTCTGGATGGAAATAGATAA
AAACAGACGGAGTCGCGAAG

Downstream 100 bases:

>100_bases
CCTTCCGGTGATGCACCTGCTAAACTCGATAAATAACAAACCGCGCCTGCCGCCGCATTCTGGCAGGCCGCTCAGAAGGA
GAAGCAATGTACCCCGTTGA

Product: NAD-binding D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: 2-ketoacid reductase

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNIIFYHPFFNAEEWLAGLSQRLPQAQVRAWQPGDDRPADYALVWRPPHEMLANRGRLKGVFALGAGVDAILEQERAYPG
TLPAGVPLLRLEDTGMAQQMQEYALSYVLRYFRRFDEYQLLQQQQEWRPLDPHQQDNFTIGILGAGVLGKSVAQKLTAFG
FNVRCWSRSPKQIDGVQSFAGAEQRAAFLDGVQLLINLLPNTPETAGILNQQLFAHLAPGAYLINLARGAHLVEDDLLQA
LEQGQLAAATLDVFVTEPLPQAHPFWRHPRVTITPHIAAITLPQAAMDQIAANILALEAGQTPAGVVDVLLGY

Sequences:

>Translated_313_residues
MNIIFYHPFFNAEEWLAGLSQRLPQAQVRAWQPGDDRPADYALVWRPPHEMLANRGRLKGVFALGAGVDAILEQERAYPG
TLPAGVPLLRLEDTGMAQQMQEYALSYVLRYFRRFDEYQLLQQQQEWRPLDPHQQDNFTIGILGAGVLGKSVAQKLTAFG
FNVRCWSRSPKQIDGVQSFAGAEQRAAFLDGVQLLINLLPNTPETAGILNQQLFAHLAPGAYLINLARGAHLVEDDLLQA
LEQGQLAAATLDVFVTEPLPQAHPFWRHPRVTITPHIAAITLPQAAMDQIAANILALEAGQTPAGVVDVLLGY
>Mature_313_residues
MNIIFYHPFFNAEEWLAGLSQRLPQAQVRAWQPGDDRPADYALVWRPPHEMLANRGRLKGVFALGAGVDAILEQERAYPG
TLPAGVPLLRLEDTGMAQQMQEYALSYVLRYFRRFDEYQLLQQQQEWRPLDPHQQDNFTIGILGAGVLGKSVAQKLTAFG
FNVRCWSRSPKQIDGVQSFAGAEQRAAFLDGVQLLINLLPNTPETAGILNQQLFAHLAPGAYLINLARGAHLVEDDLLQA
LEQGQLAAATLDVFVTEPLPQAHPFWRHPRVTITPHIAAITLPQAAMDQIAANILALEAGQTPAGVVDVLLGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily

Homologues:

Organism=Homo sapiens, GI6912396, Length=248, Percent_Identity=30.6451612903226, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI23308577, Length=266, Percent_Identity=26.6917293233083, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI61743967, Length=238, Percent_Identity=28.9915966386555, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI4557497, Length=238, Percent_Identity=28.9915966386555, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI145580578, Length=238, Percent_Identity=27.3109243697479, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI4557499, Length=238, Percent_Identity=27.3109243697479, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI145580575, Length=238, Percent_Identity=27.3109243697479, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI87081824, Length=313, Percent_Identity=59.1054313099042, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI87082289, Length=216, Percent_Identity=30.5555555555556, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17532191, Length=321, Percent_Identity=25.5451713395639, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI28574286, Length=233, Percent_Identity=24.8927038626609, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24585514, Length=256, Percent_Identity=26.953125, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28574282, Length=256, Percent_Identity=26.953125, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45552429, Length=256, Percent_Identity=26.953125, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28571528, Length=164, Percent_Identity=32.3170731707317, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28574284, Length=256, Percent_Identity=26.953125, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI45551003, Length=256, Percent_Identity=26.953125, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24646446, Length=258, Percent_Identity=26.3565891472868, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24646448, Length=258, Percent_Identity=26.3565891472868, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24646452, Length=258, Percent_Identity=26.3565891472868, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24646450, Length=258, Percent_Identity=26.3565891472868, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI62472511, Length=258, Percent_Identity=26.3565891472868, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GHRA_SERP5 (A8GD46)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001478164.1
- ProteinModelPortal:   A8GD46
- SMR:   A8GD46
- STRING:   A8GD46
- GeneID:   5606872
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1933
- eggNOG:   COG0111
- HOGENOM:   HBG731446
- OMA:   EYVTHAV
- ProtClustDB:   CLSK2785223
- BioCyc:   SPRO399741:SPRO_1933-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01666
- InterPro:   IPR006140
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: PF02826 2-Hacid_dh_C

EC number: =1.1.1.79; =1.1.1.81

Molecular weight: Translated: 34642; Mature: 34642

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1; PS00670 D_2_HYDROXYACID_DH_2; PS00671 D_2_HYDROXYACID_DH_3

Important sites: ACT_SITE 228-228 ACT_SITE 276-276

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIFYHPFFNAEEWLAGLSQRLPQAQVRAWQPGDDRPADYALVWRPPHEMLANRGRLKG
CCEEEECCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCCEEEEECCHHHHHHCCCCCCE
VFALGAGVDAILEQERAYPGTLPAGVPLLRLEDTGMAQQMQEYALSYVLRYFRRFDEYQL
EEEECCCHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LQQQQEWRPLDPHQQDNFTIGILGAGVLGKSVAQKLTAFGFNVRCWSRSPKQIDGVQSFA
HHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHH
GAEQRAAFLDGVQLLINLLPNTPETAGILNQQLFAHLAPGAYLINLARGAHLVEDDLLQA
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHEEEHHCCCHHHHHHHHHH
LEQGQLAAATLDVFVTEPLPQAHPFWRHPRVTITPHIAAITLPQAAMDQIAANILALEAG
HHCCCHHEEEEEEEEECCCCCCCCCCCCCCEEECCCEEEEECCHHHHHHHHHHHHEEECC
QTPAGVVDVLLGY
CCCHHHHHHHHCC
>Mature Secondary Structure
MNIIFYHPFFNAEEWLAGLSQRLPQAQVRAWQPGDDRPADYALVWRPPHEMLANRGRLKG
CCEEEECCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCCEEEEECCHHHHHHCCCCCCE
VFALGAGVDAILEQERAYPGTLPAGVPLLRLEDTGMAQQMQEYALSYVLRYFRRFDEYQL
EEEECCCHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LQQQQEWRPLDPHQQDNFTIGILGAGVLGKSVAQKLTAFGFNVRCWSRSPKQIDGVQSFA
HHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHH
GAEQRAAFLDGVQLLINLLPNTPETAGILNQQLFAHLAPGAYLINLARGAHLVEDDLLQA
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHEEEHHCCCHHHHHHHHHH
LEQGQLAAATLDVFVTEPLPQAHPFWRHPRVTITPHIAAITLPQAAMDQIAANILALEAG
HHCCCHHEEEEEEEEECCCCCCCCCCCCCCEEECCCEEEEECCHHHHHHHHHHHHEEECC
QTPAGVVDVLLGY
CCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA