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The map label for this gene is paaJ [H]

Identifier: 157370073

GI number: 157370073

Start: 2014092

End: 2015303

Strand: Reverse

Name: paaJ [H]

Synonym: Spro_1831

Alternate gene names: 157370073

Gene position: 2015303-2014092 (Counterclockwise)

Preceding gene: 157370074

Following gene: 157370072

Centisome position: 36.99

GC content: 60.23

Gene sequence:

>1212_bases
ATGACGATTAAAGACGATGGAGTCTATCTGATCGACGGCACGCGCACGGCTATTGGCGCCTATGGCGGTAGCCTGGCGTT
AACTCGTCCAGACGATATGGCGGCCTCTATTATCCGGGCGCTGCTGCAACGACATCCCGAAACCGAAGCGGATATTGATG
AGGTGATCCTCGGCTGCGCCAATCAGGCCGGAGAAGACAACCGTAACGTGGCCCGGATGGCCGCGCTGCTCTCCGGTCTG
GATCAGGGGGTGCCGGCGATTACCCTTAACCGTTTGTGTGCTTCCGGGCTGGATGCGGTGATCTACGCCAGCGCAAAAAT
AAAAAGCGGCCTCAGCGATCTGGTATTGGCCGGGGGTGTGGAAAGCATGAGCCGCGCGCCCTACGTGATGGGCAAAAGTG
AAACGCCTTTCGACAAGGGGGTAAAACTCTACGACACCACCCTGGGCTGGCGTTTTATCAATCCGCAGTTGGCACAGCGC
TATGGCAGCGATCCGCTGGGCATAACGGCGGAGAACGTCGCGCAGCAGTACCGGATATCCCGCGACGATCAGGATGCTTT
TGCCCTGGCCTCGCAGCAGAAGGCCTGGCGGGCGATCGAAAATGGTCGCCTGGCCAGGGAAATAACGCCGCTTGAGGTCC
AGGCCGACCGTAAAACCCGTAAAACCTTCGAACAGGATGAGTTCCCGCGTCAGACCACGCTGCAAAAATTGCAGGCGCTG
AAACCGGCCTTTACCCCCGACGGCTCGGTCACTGCCGGCAACGCCTCCGGCATCAATGACGGTGCTGCGGTGCTGTTGCT
GGCCAGCGGCCGCTATGTGAAACGCAAGTCGCTGCAGCCGCTGGCGGAGATAGGTGACAGCGCCAGCGCCGGCGTCGCCC
CTTCGCTGATGGGGATTGGCCCGGTCGCCGCCACCGATCGGCTGTTGGCGAGGTCCGGGTTTACCCTGAACGATTTTGAC
GTGATTGAAATTAATGAGGCGTTTGCCGCCCAGGTGCTGGCGACGCTGCAAAGCTGGCGTATCGACTTCAAGGATACGCG
GGTCAATCCCAACGGCGGAGCCATCGCCCTTGGGCATCCGCTGGGCATGTCCGGCGCGCGACTGGTGCTTTCCAGCGCGC
TGGAGCTGCATACCCGGCAATTAAACCATGCGCTGGTGACAATGTGTGTCGGTGTCGGCCAGGGTGTTTCATTGGTCATT
AAAGCCCTCTAA

Upstream 100 bases:

>100_bases
CAATATGGCGCAACTGCTGCATTCCGCCCGCTATTACCCGCATTACTCTTTGCTCAATACCCCCCGGCCAGAGCTGGCCG
TCGCGCCCTAAGGAAAAATG

Downstream 100 bases:

>100_bases
TGCAAGGAATTCCGTATGAGCGTATTAAACATTCTTGAAGAAAAACTGGCCGAAACCAAAAGACAGGGCCGCTTCCGTGA
GTTCCTCAATCTGGAACGCA

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MTIKDDGVYLIDGTRTAIGAYGGSLALTRPDDMAASIIRALLQRHPETEADIDEVILGCANQAGEDNRNVARMAALLSGL
DQGVPAITLNRLCASGLDAVIYASAKIKSGLSDLVLAGGVESMSRAPYVMGKSETPFDKGVKLYDTTLGWRFINPQLAQR
YGSDPLGITAENVAQQYRISRDDQDAFALASQQKAWRAIENGRLAREITPLEVQADRKTRKTFEQDEFPRQTTLQKLQAL
KPAFTPDGSVTAGNASGINDGAAVLLLASGRYVKRKSLQPLAEIGDSASAGVAPSLMGIGPVAATDRLLARSGFTLNDFD
VIEINEAFAAQVLATLQSWRIDFKDTRVNPNGGAIALGHPLGMSGARLVLSSALELHTRQLNHALVTMCVGVGQGVSLVI
KAL

Sequences:

>Translated_403_residues
MTIKDDGVYLIDGTRTAIGAYGGSLALTRPDDMAASIIRALLQRHPETEADIDEVILGCANQAGEDNRNVARMAALLSGL
DQGVPAITLNRLCASGLDAVIYASAKIKSGLSDLVLAGGVESMSRAPYVMGKSETPFDKGVKLYDTTLGWRFINPQLAQR
YGSDPLGITAENVAQQYRISRDDQDAFALASQQKAWRAIENGRLAREITPLEVQADRKTRKTFEQDEFPRQTTLQKLQAL
KPAFTPDGSVTAGNASGINDGAAVLLLASGRYVKRKSLQPLAEIGDSASAGVAPSLMGIGPVAATDRLLARSGFTLNDFD
VIEINEAFAAQVLATLQSWRIDFKDTRVNPNGGAIALGHPLGMSGARLVLSSALELHTRQLNHALVTMCVGVGQGVSLVI
KAL
>Mature_402_residues
TIKDDGVYLIDGTRTAIGAYGGSLALTRPDDMAASIIRALLQRHPETEADIDEVILGCANQAGEDNRNVARMAALLSGLD
QGVPAITLNRLCASGLDAVIYASAKIKSGLSDLVLAGGVESMSRAPYVMGKSETPFDKGVKLYDTTLGWRFINPQLAQRY
GSDPLGITAENVAQQYRISRDDQDAFALASQQKAWRAIENGRLAREITPLEVQADRKTRKTFEQDEFPRQTTLQKLQALK
PAFTPDGSVTAGNASGINDGAAVLLLASGRYVKRKSLQPLAEIGDSASAGVAPSLMGIGPVAATDRLLARSGFTLNDFDV
IEINEAFAAQVLATLQSWRIDFKDTRVNPNGGAIALGHPLGMSGARLVLSSALELHTRQLNHALVTMCVGVGQGVSLVIK
AL

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=400, Percent_Identity=40.25, Blast_Score=277, Evalue=1e-74,
Organism=Homo sapiens, GI148539872, Length=404, Percent_Identity=36.8811881188119, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI4501853, Length=401, Percent_Identity=40.1496259351621, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI4557237, Length=405, Percent_Identity=36.2962962962963, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI4504327, Length=424, Percent_Identity=30.8962264150943, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI194353979, Length=398, Percent_Identity=29.3969849246231, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1787663, Length=395, Percent_Identity=54.6835443037975, Blast_Score=434, Evalue=1e-123,
Organism=Escherichia coli, GI1788554, Length=397, Percent_Identity=44.8362720403023, Blast_Score=292, Evalue=3e-80,
Organism=Escherichia coli, GI87082165, Length=395, Percent_Identity=43.5443037974684, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI48994986, Length=399, Percent_Identity=38.3458646616541, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1788683, Length=432, Percent_Identity=28.2407407407407, Blast_Score=134, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI133906874, Length=402, Percent_Identity=39.5522388059701, Blast_Score=271, Evalue=6e-73,
Organism=Caenorhabditis elegans, GI25147385, Length=397, Percent_Identity=34.2569269521411, Blast_Score=228, Evalue=6e-60,
Organism=Caenorhabditis elegans, GI17535921, Length=405, Percent_Identity=35.8024691358025, Blast_Score=226, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI17551802, Length=424, Percent_Identity=31.1320754716981, Blast_Score=177, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI17535917, Length=408, Percent_Identity=28.921568627451, Blast_Score=144, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6322031, Length=415, Percent_Identity=36.144578313253, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6325229, Length=401, Percent_Identity=35.1620947630923, Blast_Score=213, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24655093, Length=397, Percent_Identity=41.8136020151133, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI17648125, Length=410, Percent_Identity=37.3170731707317, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24640423, Length=397, Percent_Identity=34.7607052896725, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI17137578, Length=425, Percent_Identity=30.1176470588235, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI19921506, Length=356, Percent_Identity=25.8426966292135, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 42886; Mature: 42755

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKDDGVYLIDGTRTAIGAYGGSLALTRPDDMAASIIRALLQRHPETEADIDEVILGCA
CCCCCCCEEEECCCCHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
NQAGEDNRNVARMAALLSGLDQGVPAITLNRLCASGLDAVIYASAKIKSGLSDLVLAGGV
CCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEHHHHHCCHHHHHHHCCH
ESMSRAPYVMGKSETPFDKGVKLYDTTLGWRFINPQLAQRYGSDPLGITAENVAQQYRIS
HHHHCCCEEECCCCCCHHCCCEEEEEECCEEECCHHHHHHCCCCCCCCCHHHHHHHHHCC
RDDQDAFALASQQKAWRAIENGRLAREITPLEVQADRKTRKTFEQDEFPRQTTLQKLQAL
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHCCCHHHHHHHHHHH
KPAFTPDGSVTAGNASGINDGAAVLLLASGRYVKRKSLQPLAEIGDSASAGVAPSLMGIG
CCCCCCCCCEECCCCCCCCCCEEEEEEECCCHHHHHCCCHHHHCCCCCCCCCCCHHHCCC
PVAATDRLLARSGFTLNDFDVIEINEAFAAQVLATLQSWRIDFKDTRVNPNGGAIALGHP
CHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCEECCCCCEEEECCC
LGMSGARLVLSSALELHTRQLNHALVTMCVGVGQGVSLVIKAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEECC
>Mature Secondary Structure 
TIKDDGVYLIDGTRTAIGAYGGSLALTRPDDMAASIIRALLQRHPETEADIDEVILGCA
CCCCCCEEEECCCCHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
NQAGEDNRNVARMAALLSGLDQGVPAITLNRLCASGLDAVIYASAKIKSGLSDLVLAGGV
CCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEHHHHHCCHHHHHHHCCH
ESMSRAPYVMGKSETPFDKGVKLYDTTLGWRFINPQLAQRYGSDPLGITAENVAQQYRIS
HHHHCCCEEECCCCCCHHCCCEEEEEECCEEECCHHHHHHCCCCCCCCCHHHHHHHHHCC
RDDQDAFALASQQKAWRAIENGRLAREITPLEVQADRKTRKTFEQDEFPRQTTLQKLQAL
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHCCCHHHHHHHHHHH
KPAFTPDGSVTAGNASGINDGAAVLLLASGRYVKRKSLQPLAEIGDSASAGVAPSLMGIG
CCCCCCCCCEECCCCCCCCCCEEEEEEECCCHHHHHCCCHHHHCCCCCCCCCCCHHHCCC
PVAATDRLLARSGFTLNDFDVIEINEAFAAQVLATLQSWRIDFKDTRVNPNGGAIALGHP
CHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCEECCCCCEEEECCC
LGMSGARLVLSSALELHTRQLNHALVTMCVGVGQGVSLVIKAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 10766858 [H]