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The map label for this gene is rutA

Identifier: 157370065

GI number: 157370065

Start: 2005733

End: 2006833

Strand: Reverse

Name: rutA

Synonym: Spro_1823

Alternate gene names: 157370065

Gene position: 2006833-2005733 (Counterclockwise)

Preceding gene: 157370067

Following gene: 157370064

Centisome position: 36.83

GC content: 58.22

Gene sequence:

>1101_bases
ATGAAAATTGGCGTATTCATCCCGATCGGCAACAACGGCTGGCTGATTTCCAGCAATGCCCCGCAGTACCAACCGACGTT
TGAACTCAATAAAACCATCGTGCAGAAGGCCGAGCACTACAACTTCGATTTTGCCCTGTCGATGATCAAACTGCGCGGTT
TCGGTGGCAAAACCGAATTCTGGGATCACAACCTGGAGTCCTTCACCCTGATGGCCGGGCTGGCCGCCGTCACCTCACGC
ATCAAGATCTACGCCACTGCCGCCACTCTGACCATGCCACCGGCGATCGTCGCCCGCATGGCCTCCACCATTGACTCGAT
TTCCAACGGGCGTTTTGGCCTTAATGTAGTGACGGGCTGGCAGAAGCCGGAATACGAGCAGATGGGCATGTGGCCGGGCG
ACGATTACTTCGGCCGTCGCTACGACTATCTGGCGGAGTACGTCAATGTGCTGCGCGATCTGTGGGGTACCGGTAAATCC
GATTTCAAAGGCGAGTTTTTCCAGATGGACGACTGTCGCGTCAGCCCACAGCCGCAGGCGGACATCAAGGTGATCTGCGC
CGGACAAAGCGACGCGGGCATGGCCTTCTCGGCCAAATATGCCGATTACAACTTCTGCTTTGGCAAGGGGGTCAACACGC
CGACCGCCTTTGCACCAACCGCAGCACGCTTAAAAATTGCGGCGGATAGCGAAGGCCGCAGCGTGGCCTGCTACGTGCTG
TTTATGATTATTGCCGATGAAACCGACGAGGCCGCCCGCGCCAAGTGGGAAAGCTATAAAGCAGGGGCCGATACCGAAGC
CCTGGCCTGGCTGACCGAGCAAAGCGGCAAGGACACTAAATCCGGTGCCGACACCAACGTACGCCAGATGGCGGACCCCA
CCTCCGCCGTCAATATCAATATGGGCACGCTGGTCGGTTCCTACGCTAACGTGGCGCGCATGATGGATGAAATCGCCACC
GTGCCCGGCACCGAAGGTGTGCTGCTGACCTTCGATGACTTCATCACCGGGGTGGAAAACTTTGGCGAGCGCATCCAGCC
GCTAATGAAAAGCCGCGCCGACGTTTGCCCACAGACTGCCGCAAGCCGCGAGGTGGCATAA

Upstream 100 bases:

>100_bases
AAACTGGCACCGCCTTTGCAAAAGCCTCCGTATCCAGCGCCGTCCACAGAAAGCATTCAGGCGACGCATGATCCGTTCCA
CTAGCAACCGAGGTGTTCAA

Downstream 100 bases:

>100_bases
TGAAAATTGTTGAAAACCAACCGGTAGTACGCCGTCAGTCGCCGCAGGCCGAGGTTACGCTCACCCTGCCCGCCCGTCCG
GAGGCTATCGCCTTTGCGCC

Product: luciferase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKIGVFIPIGNNGWLISSNAPQYQPTFELNKTIVQKAEHYNFDFALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSR
IKIYATAATLTMPPAIVARMASTIDSISNGRFGLNVVTGWQKPEYEQMGMWPGDDYFGRRYDYLAEYVNVLRDLWGTGKS
DFKGEFFQMDDCRVSPQPQADIKVICAGQSDAGMAFSAKYADYNFCFGKGVNTPTAFAPTAARLKIAADSEGRSVACYVL
FMIIADETDEAARAKWESYKAGADTEALAWLTEQSGKDTKSGADTNVRQMADPTSAVNINMGTLVGSYANVARMMDEIAT
VPGTEGVLLTFDDFITGVENFGERIQPLMKSRADVCPQTAASREVA

Sequences:

>Translated_366_residues
MKIGVFIPIGNNGWLISSNAPQYQPTFELNKTIVQKAEHYNFDFALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSR
IKIYATAATLTMPPAIVARMASTIDSISNGRFGLNVVTGWQKPEYEQMGMWPGDDYFGRRYDYLAEYVNVLRDLWGTGKS
DFKGEFFQMDDCRVSPQPQADIKVICAGQSDAGMAFSAKYADYNFCFGKGVNTPTAFAPTAARLKIAADSEGRSVACYVL
FMIIADETDEAARAKWESYKAGADTEALAWLTEQSGKDTKSGADTNVRQMADPTSAVNINMGTLVGSYANVARMMDEIAT
VPGTEGVLLTFDDFITGVENFGERIQPLMKSRADVCPQTAASREVA
>Mature_366_residues
MKIGVFIPIGNNGWLISSNAPQYQPTFELNKTIVQKAEHYNFDFALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSR
IKIYATAATLTMPPAIVARMASTIDSISNGRFGLNVVTGWQKPEYEQMGMWPGDDYFGRRYDYLAEYVNVLRDLWGTGKS
DFKGEFFQMDDCRVSPQPQADIKVICAGQSDAGMAFSAKYADYNFCFGKGVNTPTAFAPTAARLKIAADSEGRSVACYVL
FMIIADETDEAARAKWESYKAGADTEALAWLTEQSGKDTKSGADTNVRQMADPTSAVNINMGTLVGSYANVARMMDEIAT
VPGTEGVLLTFDDFITGVENFGERIQPLMKSRADVCPQTAASREVA

Specific function: Catalyzes the pyrimidine ring opening between N-3 and C- 4 by an unusual flavin hydroperoxide-catalyzed mechanism to yield ureidoacrylate peracid. It cleaves pyrmidine rings directly by adding oxygen atoms, making a toxic ureidoacrylate peracid product wh

COG id: COG2141

COG function: function code C; Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ntaA/snaA/soxA(dszA) monooxygenase family. RutA subfamily

Homologues:

Organism=Escherichia coli, GI1787247, Length=366, Percent_Identity=83.3333333333333, Blast_Score=652, Evalue=0.0,
Organism=Escherichia coli, GI1787166, Length=316, Percent_Identity=23.1012658227848, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUTA_SERP5 (A8GCT6)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001478054.1
- ProteinModelPortal:   A8GCT6
- STRING:   A8GCT6
- GeneID:   5605420
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1823
- eggNOG:   COG2141
- HOGENOM:   HBG350183
- OMA:   FIPIGNN
- ProtClustDB:   CLSK879925
- BioCyc:   SPRO399741:SPRO_1823-MONOMER
- HAMAP:   MF_01699
- InterPro:   IPR011251
- InterPro:   IPR019914
- InterPro:   IPR016048
- Gene3D:   G3DSA:3.20.20.30
- TIGRFAMs:   TIGR03612

Pfam domain/function: PF00296 Bac_luciferase; SSF51679 Luciferase_like

EC number: NA

Molecular weight: Translated: 40118; Mature: 40118

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: BINDING 115-115 BINDING 124-124 BINDING 190-190

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGVFIPIGNNGWLISSNAPQYQPTFELNKTIVQKAEHYNFDFALSMIKLRGFGGKTEF
CEEEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHEEEEHEECCCCCCCC
WDHNLESFTLMAGLAAVTSRIKIYATAATLTMPPAIVARMASTIDSISNGRFGLNVVTGW
CCCCCHHHHHHHHHHHHHHCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECC
QKPEYEQMGMWPGDDYFGRRYDYLAEYVNVLRDLWGTGKSDFKGEFFQMDDCRVSPQPQA
CCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCC
DIKVICAGQSDAGMAFSAKYADYNFCFGKGVNTPTAFAPTAARLKIAADSEGRSVACYVL
CEEEEEECCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEHHHEE
FMIIADETDEAARAKWESYKAGADTEALAWLTEQSGKDTKSGADTNVRQMADPTSAVNIN
EEHHCCCCCHHHHHHHHHHCCCCCHHHHHEEHHCCCCCCCCCCCCCHHHHCCCCCEEEEE
MGTLVGSYANVARMMDEIATVPGTEGVLLTFDDFITGVENFGERIQPLMKSRADVCPQTA
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ASREVA
CCCCCC
>Mature Secondary Structure
MKIGVFIPIGNNGWLISSNAPQYQPTFELNKTIVQKAEHYNFDFALSMIKLRGFGGKTEF
CEEEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHEEEEHEECCCCCCCC
WDHNLESFTLMAGLAAVTSRIKIYATAATLTMPPAIVARMASTIDSISNGRFGLNVVTGW
CCCCCHHHHHHHHHHHHHHCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECC
QKPEYEQMGMWPGDDYFGRRYDYLAEYVNVLRDLWGTGKSDFKGEFFQMDDCRVSPQPQA
CCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCC
DIKVICAGQSDAGMAFSAKYADYNFCFGKGVNTPTAFAPTAARLKIAADSEGRSVACYVL
CEEEEEECCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEHHHEE
FMIIADETDEAARAKWESYKAGADTEALAWLTEQSGKDTKSGADTNVRQMADPTSAVNIN
EEHHCCCCCHHHHHHHHHHCCCCCHHHHHEEHHCCCCCCCCCCCCCHHHHCCCCCEEEEE
MGTLVGSYANVARMMDEIATVPGTEGVLLTFDDFITGVENFGERIQPLMKSRADVCPQTA
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ASREVA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA