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The map label for this gene is rutB

Identifier: 157370064

GI number: 157370064

Start: 2004987

End: 2005733

Strand: Reverse

Name: rutB

Synonym: Spro_1822

Alternate gene names: 157370064

Gene position: 2005733-2004987 (Counterclockwise)

Preceding gene: 157370065

Following gene: 157370063

Centisome position: 36.81

GC content: 59.44

Gene sequence:

>747_bases
ATGAAAATTGTTGAAAACCAACCGGTAGTACGCCGTCAGTCGCCGCAGGCCGAGGTTACGCTCACCCTGCCCGCCCGTCC
GGAGGCTATCGCCTTTGCGCCGCAGGAAACCGCGCTGATCGTGGTGGATATGCAAAATGCCTACGCGTCGCAGGGCGGCT
ATCTGGATCTGGCCGGTTTTGACATCTCGGCCACCGCCCCGGTGATCGCCAACATCAAACGCGCTATCAGCGCTGCCCGT
GCCGCTGGTATCAAGGTGATCTTTTTCCAAAATGGCTGGGACAACCAGTACGTCGAAGCCGGTGGGCAGGGTTCCCCCAA
CTGGCATAAATCCAACGCGCTGAAAACCATGCGTAAACGCCCGGAACTGATGGGCAAGCTGCTGGCCAGGGGCGACTGGG
ATTACGATCTGGTGGACGAGCTGCAACCGCAAGCCGGCGATATCGTGCTGCCGAAACCGCGCTACAGCGGCTTTTTCAAC
ACCCAACTCGACAGCCTGCTGCGCTCTTACGGCATTCATCATCTGGTGTTTACCGGCATTGCCACCAACGTCTGTGTCGA
GTCCACGCTGCGCGACGGTTTTTTTCTCGAGTATTTCGGCATCGTGCTGGCCGACGCCACTCACCAGGCCGGGCCACAGT
TTGCCCAGCAGGCGGCGCTGTACAACATCGAAACCTTCTTTGGCTGGGTGTCGGACGTCGACAGTTTCTGTAATACGCTC
GCCGCCCCGCTCAGCCAGACGGCATGA

Upstream 100 bases:

>100_bases
TCATCACCGGGGTGGAAAACTTTGGCGAGCGCATCCAGCCGCTAATGAAAAGCCGCGCCGACGTTTGCCCACAGACTGCC
GCAAGCCGCGAGGTGGCATA

Downstream 100 bases:

>100_bases
GTGTTGCACGCCTTTAACCAAAATTGAATTTTCACCAGGAGCGCCACTATGCCAAAAACCATTATTACCCCGCCGGGCAC
CGGCAAACCGCTGGCCCCTT

Product: isochorismatase hydrolase

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MKIVENQPVVRRQSPQAEVTLTLPARPEAIAFAPQETALIVVDMQNAYASQGGYLDLAGFDISATAPVIANIKRAISAAR
AAGIKVIFFQNGWDNQYVEAGGQGSPNWHKSNALKTMRKRPELMGKLLARGDWDYDLVDELQPQAGDIVLPKPRYSGFFN
TQLDSLLRSYGIHHLVFTGIATNVCVESTLRDGFFLEYFGIVLADATHQAGPQFAQQAALYNIETFFGWVSDVDSFCNTL
AAPLSQTA

Sequences:

>Translated_248_residues
MKIVENQPVVRRQSPQAEVTLTLPARPEAIAFAPQETALIVVDMQNAYASQGGYLDLAGFDISATAPVIANIKRAISAAR
AAGIKVIFFQNGWDNQYVEAGGQGSPNWHKSNALKTMRKRPELMGKLLARGDWDYDLVDELQPQAGDIVLPKPRYSGFFN
TQLDSLLRSYGIHHLVFTGIATNVCVESTLRDGFFLEYFGIVLADATHQAGPQFAQQAALYNIETFFGWVSDVDSFCNTL
AAPLSQTA
>Mature_248_residues
MKIVENQPVVRRQSPQAEVTLTLPARPEAIAFAPQETALIVVDMQNAYASQGGYLDLAGFDISATAPVIANIKRAISAAR
AAGIKVIFFQNGWDNQYVEAGGQGSPNWHKSNALKTMRKRPELMGKLLARGDWDYDLVDELQPQAGDIVLPKPRYSGFFN
TQLDSLLRSYGIHHLVFTGIATNVCVESTLRDGFFLEYFGIVLADATHQAGPQFAQQAALYNIETFFGWVSDVDSFCNTL
AAPLSQTA

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily

Homologues:

Organism=Escherichia coli, GI87081820, Length=220, Percent_Identity=80.4545454545455, Blast_Score=379, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUTB_SERP5 (A8GCT5)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001478053.1
- ProteinModelPortal:   A8GCT5
- SMR:   A8GCT5
- STRING:   A8GCT5
- GeneID:   5605419
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1822
- eggNOG:   COG1335
- HOGENOM:   HBG752434
- OMA:   MQNAYAS
- ProtClustDB:   CLSK879924
- BioCyc:   SPRO399741:SPRO_1822-MONOMER
- HAMAP:   MF_00830
- InterPro:   IPR019916
- InterPro:   IPR000868
- Gene3D:   G3DSA:3.40.50.850
- TIGRFAMs:   TIGR03614

Pfam domain/function: PF00857 Isochorismatase; SSF52499 Iscrsm_hydrolase

EC number: NA

Molecular weight: Translated: 27186; Mature: 27186

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: ACT_SITE 185-185

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVENQPVVRRQSPQAEVTLTLPARPEAIAFAPQETALIVVDMQNAYASQGGYLDLAGF
CCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCCCEEEEEECCHHHHCCCCEEEEECC
DISATAPVIANIKRAISAARAAGIKVIFFQNGWDNQYVEAGGQGSPNWHKSNALKTMRKR
CCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHCCCCCCCCCCHHHHHHHHHHC
PELMGKLLARGDWDYDLVDELQPQAGDIVLPKPRYSGFFNTQLDSLLRSYGIHHLVFTGI
HHHHHHHHHCCCCCHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
ATNVCVESTLRDGFFLEYFGIVLADATHQAGPQFAQQAALYNIETFFGWVSDVDSFCNTL
HHHHHHHHHHCCCHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAPLSQTA
HHHHHCCC
>Mature Secondary Structure
MKIVENQPVVRRQSPQAEVTLTLPARPEAIAFAPQETALIVVDMQNAYASQGGYLDLAGF
CCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCCCEEEEEECCHHHHCCCCEEEEECC
DISATAPVIANIKRAISAARAAGIKVIFFQNGWDNQYVEAGGQGSPNWHKSNALKTMRKR
CCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHCCCCCCCCCCHHHHHHHHHHC
PELMGKLLARGDWDYDLVDELQPQAGDIVLPKPRYSGFFNTQLDSLLRSYGIHHLVFTGI
HHHHHHHHHCCCCCHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
ATNVCVESTLRDGFFLEYFGIVLADATHQAGPQFAQQAALYNIETFFGWVSDVDSFCNTL
HHHHHHHHHHCCCHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAPLSQTA
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA