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The map label for this gene is aprF [H]

Identifier: 157369841

GI number: 157369841

Start: 1749485

End: 1750864

Strand: Direct

Name: aprF [H]

Synonym: Spro_1598

Alternate gene names: 157369841

Gene position: 1749485-1750864 (Clockwise)

Preceding gene: 157369840

Following gene: 157369842

Centisome position: 32.11

GC content: 52.61

Gene sequence:

>1380_bases
TTGATTCAATTTAAACGACAGGTTGCTGGCCTTGCTATCAGTACCCTCTTGTTTGCGATAGCTGCGCCGGTTCATTCGAT
AGGGATTATAGACGCATATTCGCTGGCACTTGAAAAAGATCCGACCTTTAGGGCGGCTATAAAAGAGAAAGAGGCGGGAG
ACGAAAACGAAAACATCGGCAGGGCGGGGCTGCTGCCGAAGGTATCGCTTAATTACCAGAACTCACCGAGAAACTGGCAA
ACGCAAAAGTACCCGGCGAGTGACATTTTTGGCAACAGTCTTGGTGAAACCACCAAGCGGCAGCAGTACCGCAGTTATTC
CAGCTCGGTAACGCTGACGCAACCGCTGTTCGATTACGAGGCTTATGCACGCTACAAGGCTGGCGTGGCGCAGACGCTGA
TGTCTGATGAGCGCTACCGCAGCAAGTTTCTTGATTTGGCCGTACGGGTGATCTCCGCCTATGTGGAAGTGGCTTACTCG
AAGGATCAAATCGCTTTAGCTTCAGCTCAAAAGGCGGCCTATAAAGAACAGTTGTCGCTGAACGATCGTTTAATGAGCGC
GGGTGAGGGCACCATTACCGACGTTTCCGAGACTCAGGCGCGCTACAGCTTGGCAGAGGCGCAGGAAATTGAGGCGCGTG
ATGCGCTGGACGCGGCTCAGCGCGAGTTGGAAGTCATTATTGGTATGCCGCTCAATCAGCTCGATGACGTACAGCTGCTA
CGCCCTGGCAAATTCCAGGTGGCGCCGTTAATCCCATCAAAGTTTGAAGAGTGGCAAAAAATTGCCCTCGAGAATAATCC
GATGTTGGCGGCCTCTCGCCACGGGGTTGATGCGGCGAAATATGAAGTGGAGAGAAATCGTGCTGGTTTTATGCCGCAGG
TGCAGCTCTACGCCTCGCATTCTGAGAATGACTCCAGCAGCGATAATACGGTTAACCAAAAATATCGCACTGACAGTATT
GGTGTACAGGTGAGTATGCCGATCTACGCCGGCGGTGGGGTTTCGGCATCGACTCGCCAGGCTGCATCACGATACGGGCA
GGCGATGTACGAAATGGATGCGCAGGTGGGCACTACGCTTAACGATCTGCGCAAACAGTATAATTTGTGCATTAGCAGCC
GTGCCAAATTGGCGGCTTACGAGCTGGCGGTGAAGTCGGCGACCACTCAGGTAACGGCAACCCGGCAAAGCGTATTGGCG
GGGCAACGTGTCAACGTCGATGTACTTAATGCTGAGCAACAGTTGTACAGTGCCCAACGTGATCTGGCCTCGGCTAAGTA
CACCTACATAAAATCCTGGATTACGTTGCTGAGTGATTCGGGAACCCTTGATGAAAGAGATGTAAAACGCGTGGCGCAGT
ACTTTTCCATGAGCCGCTGA

Upstream 100 bases:

>100_bases
CTGTGGAGGTGTTTGTGAAGACCGGCTCGCGCTCACTGTTGAGTTATCTGTTTAAACCTATTTTGGATCGCGCTCATACT
TCATTAACCGAGGAATAATT

Downstream 100 bases:

>100_bases
GCAAGTAGTGGGAAATGGTTCAAATACTCACCATGAGTATTTGTCTGAAAGAGCAGCCCTGAGCTGCTCTTTTTTGTCTC
AATACGTTATGTTAGCAACT

Product: TolC family type I secretion outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MIQFKRQVAGLAISTLLFAIAAPVHSIGIIDAYSLALEKDPTFRAAIKEKEAGDENENIGRAGLLPKVSLNYQNSPRNWQ
TQKYPASDIFGNSLGETTKRQQYRSYSSSVTLTQPLFDYEAYARYKAGVAQTLMSDERYRSKFLDLAVRVISAYVEVAYS
KDQIALASAQKAAYKEQLSLNDRLMSAGEGTITDVSETQARYSLAEAQEIEARDALDAAQRELEVIIGMPLNQLDDVQLL
RPGKFQVAPLIPSKFEEWQKIALENNPMLAASRHGVDAAKYEVERNRAGFMPQVQLYASHSENDSSSDNTVNQKYRTDSI
GVQVSMPIYAGGGVSASTRQAASRYGQAMYEMDAQVGTTLNDLRKQYNLCISSRAKLAAYELAVKSATTQVTATRQSVLA
GQRVNVDVLNAEQQLYSAQRDLASAKYTYIKSWITLLSDSGTLDERDVKRVAQYFSMSR

Sequences:

>Translated_459_residues
MIQFKRQVAGLAISTLLFAIAAPVHSIGIIDAYSLALEKDPTFRAAIKEKEAGDENENIGRAGLLPKVSLNYQNSPRNWQ
TQKYPASDIFGNSLGETTKRQQYRSYSSSVTLTQPLFDYEAYARYKAGVAQTLMSDERYRSKFLDLAVRVISAYVEVAYS
KDQIALASAQKAAYKEQLSLNDRLMSAGEGTITDVSETQARYSLAEAQEIEARDALDAAQRELEVIIGMPLNQLDDVQLL
RPGKFQVAPLIPSKFEEWQKIALENNPMLAASRHGVDAAKYEVERNRAGFMPQVQLYASHSENDSSSDNTVNQKYRTDSI
GVQVSMPIYAGGGVSASTRQAASRYGQAMYEMDAQVGTTLNDLRKQYNLCISSRAKLAAYELAVKSATTQVTATRQSVLA
GQRVNVDVLNAEQQLYSAQRDLASAKYTYIKSWITLLSDSGTLDERDVKRVAQYFSMSR
>Mature_459_residues
MIQFKRQVAGLAISTLLFAIAAPVHSIGIIDAYSLALEKDPTFRAAIKEKEAGDENENIGRAGLLPKVSLNYQNSPRNWQ
TQKYPASDIFGNSLGETTKRQQYRSYSSSVTLTQPLFDYEAYARYKAGVAQTLMSDERYRSKFLDLAVRVISAYVEVAYS
KDQIALASAQKAAYKEQLSLNDRLMSAGEGTITDVSETQARYSLAEAQEIEARDALDAAQRELEVIIGMPLNQLDDVQLL
RPGKFQVAPLIPSKFEEWQKIALENNPMLAASRHGVDAAKYEVERNRAGFMPQVQLYASHSENDSSSDNTVNQKYRTDSI
GVQVSMPIYAGGGVSASTRQAASRYGQAMYEMDAQVGTTLNDLRKQYNLCISSRAKLAAYELAVKSATTQVTATRQSVLA
GQRVNVDVLNAEQQLYSAQRDLASAKYTYIKSWITLLSDSGTLDERDVKRVAQYFSMSR

Specific function: Involved in the secretion of alkaline protease [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=439, Percent_Identity=26.6514806378132, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50920; Mature: 50920

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQFKRQVAGLAISTLLFAIAAPVHSIGIIDAYSLALEKDPTFRAAIKEKEAGDENENIG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCC
RAGLLPKVSLNYQNSPRNWQTQKYPASDIFGNSLGETTKRQQYRSYSSSVTLTQPLFDYE
CCCCCCEEEECCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHCCCEEEECHHHHHH
AYARYKAGVAQTLMSDERYRSKFLDLAVRVISAYVEVAYSKDQIALASAQKAAYKEQLSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
NDRLMSAGEGTITDVSETQARYSLAEAQEIEARDALDAAQRELEVIIGMPLNQLDDVQLL
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEE
RPGKFQVAPLIPSKFEEWQKIALENNPMLAASRHGVDAAKYEVERNRAGFMPQVQLYASH
CCCCEEEECCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCCCCCEEEEEECC
SENDSSSDNTVNQKYRTDSIGVQVSMPIYAGGGVSASTRQAASRYGQAMYEMDAQVGTTL
CCCCCCCCCCHHHHEECCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCH
NDLRKQYNLCISSRAKLAAYELAVKSATTQVTATRQSVLAGQRVNVDVLNAEQQLYSAQR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHH
DLASAKYTYIKSWITLLSDSGTLDERDVKRVAQYFSMSR
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIQFKRQVAGLAISTLLFAIAAPVHSIGIIDAYSLALEKDPTFRAAIKEKEAGDENENIG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCC
RAGLLPKVSLNYQNSPRNWQTQKYPASDIFGNSLGETTKRQQYRSYSSSVTLTQPLFDYE
CCCCCCEEEECCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHCCCEEEECHHHHHH
AYARYKAGVAQTLMSDERYRSKFLDLAVRVISAYVEVAYSKDQIALASAQKAAYKEQLSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
NDRLMSAGEGTITDVSETQARYSLAEAQEIEARDALDAAQRELEVIIGMPLNQLDDVQLL
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEE
RPGKFQVAPLIPSKFEEWQKIALENNPMLAASRHGVDAAKYEVERNRAGFMPQVQLYASH
CCCCEEEECCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCCCCCEEEEEECC
SENDSSSDNTVNQKYRTDSIGVQVSMPIYAGGGVSASTRQAASRYGQAMYEMDAQVGTTL
CCCCCCCCCCHHHHEECCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCH
NDLRKQYNLCISSRAKLAAYELAVKSATTQVTATRQSVLAGQRVNVDVLNAEQQLYSAQR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHH
DLASAKYTYIKSWITLLSDSGTLDERDVKRVAQYFSMSR
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1427098; 10984043 [H]