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The map label for this gene is rfbM [H]

Identifier: 157369842

GI number: 157369842

Start: 1751171

End: 1752595

Strand: Direct

Name: rfbM [H]

Synonym: Spro_1599

Alternate gene names: 157369842

Gene position: 1751171-1752595 (Clockwise)

Preceding gene: 157369841

Following gene: 157369843

Centisome position: 32.14

GC content: 46.25

Gene sequence:

>1425_bases
ATGTCAAAACTAATTCCTGTGGTTATGGCCGGTGGTACAGGTAGCCGGTTGTGGCCACTGTCGCGCGAATCATTCCCCAA
ACAATTTCTCTCCATTGATGACAGTGGGTTCAGTTTGCTACAGCAAACTCTGCAACGTTTGTCAGGGTTGGATGGAGTCG
AAGTCGCCGCACCTTTGGTTATCTGCAATGAGAATCATCGTTTTCTGGTAGCGGAGCAACTGCGTGAAATTGATCAGCTT
GCGACCAACATTATTTTAGAGCCTATTGGGCGTAACACTGCGCCTGCTGTTGCATTGGCAGCACATTTGGCGGCTGAAGA
AGACGATGACAGCATACTTTTGGTGTTGGCCGCAGATCATTTGATTAAAAAAGTGGAAAACTTCCATGCGGCTATAAAAA
CGGCAATTATGCATGCCGCCGAAGATAACTTGGTGACTTTTGGTATTATTCCTGGGCATCCGGAAACGGGTTACGGCTAT
ATCAAGCGTGGTACTGCACTTTCCAGCCAATGCTTCAAAGTTGATGCATTTGTGGAGAAACCTTGCTTGGAAAAGGCAAC
TGAGTATCTTGCCAGTGGTGAGTTTAGTTGGAACAGCGGCATGTTCATGTTTAAAACTGCCAAATTCCTGCAAGAGCTGC
AACAGTTCAGCCCTGAAATATTCAGTACAACACAACAATCTGTGGTCGACAGCCAGCGTGATATGAATTTTATCCGTGTT
GATCAAGAAATATTTAAGCATTGCCCAAGTGACTCCATCGATTATGCGGTGATGGAAAAAACACGTGATGCAGTGGTGAT
CCCCATAGATGCCGGCTGGAGCGATGTAGGTTCATGGACATCTCTGTGGGACGTTTCAGAAAAGGATGCTCTCGGGAATG
TTAACGTCGGTGAGATTATCTCCATTGATTCCAATAATAACTACATCTCATCTGATGGCGCGCTAGTTGCTACTATCGGG
CTGGATGACCTTATCGTGATCAATACCAATGATGCGTTGTTGATTTCGACTAAGGATCGCGTTCAGGACGTTAAGAAAGT
GGTTGATGAGCTAAAAAAACGCAACCTTCATCACTTTCGCCAGCACTCCTCTGCTTATCGCCCATGGGGCAAGATCTGTG
ATATCGACAACGGCGAGCATTTCCAGGTCAAAAAAATCATTGTTCGCCCTGGTGAGGGATTGTCAGTACAGCGCCATCTT
CATCGCGCAGAGCACTGGGTCGTCGTTAGCGGTACGGCAAAGGTGAATGTTGAAGATAAAGAATTCTTCTTATCAGAGAA
CCAGTCTACATTCATCCCTGCTGGCAGTGTCCACACCCTGGAGAACCCTGGGAAAATCGATCTTGAAATCATTGAGGTTC
GATCAGGATTTTACCTGGAAGAAGATGACATTGAGCGTTTACATGATCGGTACGGCCGAGTTTAA

Upstream 100 bases:

>100_bases
TAATTTACGTGTGTGATTGTATGACTTTTATAATGCTGGGTTGGCAGGCGCTACGCCGCACTCTCGTTTATAAGACAGAC
GACTGAGTTAAGGGTTAATA

Downstream 100 bases:

>100_bases
TTAAAGGATTTGGTGATGGAAAAGTTAGCCTGTTTTAAAGCTTATGATGTGCGTGGTAAGTTGGGTGAGGAACTGAATGA
TGATATCGCCTACCGTATTG

Product: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase

Products: NA

Alternate protein names: GDP-mannose pyrophosphorylase; GMP; GMPP; GTP--mannose-1-phosphate guanylyltransferase [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSKLIPVVMAGGTGSRLWPLSRESFPKQFLSIDDSGFSLLQQTLQRLSGLDGVEVAAPLVICNENHRFLVAEQLREIDQL
ATNIILEPIGRNTAPAVALAAHLAAEEDDDSILLVLAADHLIKKVENFHAAIKTAIMHAAEDNLVTFGIIPGHPETGYGY
IKRGTALSSQCFKVDAFVEKPCLEKATEYLASGEFSWNSGMFMFKTAKFLQELQQFSPEIFSTTQQSVVDSQRDMNFIRV
DQEIFKHCPSDSIDYAVMEKTRDAVVIPIDAGWSDVGSWTSLWDVSEKDALGNVNVGEIISIDSNNNYISSDGALVATIG
LDDLIVINTNDALLISTKDRVQDVKKVVDELKKRNLHHFRQHSSAYRPWGKICDIDNGEHFQVKKIIVRPGEGLSVQRHL
HRAEHWVVVSGTAKVNVEDKEFFLSENQSTFIPAGSVHTLENPGKIDLEIIEVRSGFYLEEDDIERLHDRYGRV

Sequences:

>Translated_474_residues
MSKLIPVVMAGGTGSRLWPLSRESFPKQFLSIDDSGFSLLQQTLQRLSGLDGVEVAAPLVICNENHRFLVAEQLREIDQL
ATNIILEPIGRNTAPAVALAAHLAAEEDDDSILLVLAADHLIKKVENFHAAIKTAIMHAAEDNLVTFGIIPGHPETGYGY
IKRGTALSSQCFKVDAFVEKPCLEKATEYLASGEFSWNSGMFMFKTAKFLQELQQFSPEIFSTTQQSVVDSQRDMNFIRV
DQEIFKHCPSDSIDYAVMEKTRDAVVIPIDAGWSDVGSWTSLWDVSEKDALGNVNVGEIISIDSNNNYISSDGALVATIG
LDDLIVINTNDALLISTKDRVQDVKKVVDELKKRNLHHFRQHSSAYRPWGKICDIDNGEHFQVKKIIVRPGEGLSVQRHL
HRAEHWVVVSGTAKVNVEDKEFFLSENQSTFIPAGSVHTLENPGKIDLEIIEVRSGFYLEEDDIERLHDRYGRV
>Mature_473_residues
SKLIPVVMAGGTGSRLWPLSRESFPKQFLSIDDSGFSLLQQTLQRLSGLDGVEVAAPLVICNENHRFLVAEQLREIDQLA
TNIILEPIGRNTAPAVALAAHLAAEEDDDSILLVLAADHLIKKVENFHAAIKTAIMHAAEDNLVTFGIIPGHPETGYGYI
KRGTALSSQCFKVDAFVEKPCLEKATEYLASGEFSWNSGMFMFKTAKFLQELQQFSPEIFSTTQQSVVDSQRDMNFIRVD
QEIFKHCPSDSIDYAVMEKTRDAVVIPIDAGWSDVGSWTSLWDVSEKDALGNVNVGEIISIDSNNNYISSDGALVATIGL
DDLIVINTNDALLISTKDRVQDVKKVVDELKKRNLHHFRQHSSAYRPWGKICDIDNGEHFQVKKIIVRPGEGLSVQRHLH
RAEHWVVVSGTAKVNVEDKEFFLSENQSTFIPAGSVHTLENPGKIDLEIIEVRSGFYLEEDDIERLHDRYGRV

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS group C2 O antigen [H]

COG id: COG0836

COG function: function code M; Mannose-1-phosphate guanylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788362, Length=479, Percent_Identity=55.5323590814196, Blast_Score=541, Evalue=1e-155,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR006375
- InterPro:   IPR001538
- InterPro:   IPR005835
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01050 MannoseP_isomer; PF00483 NTP_transferase [H]

EC number: =2.7.7.13 [H]

Molecular weight: Translated: 52858; Mature: 52727

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLIPVVMAGGTGSRLWPLSRESFPKQFLSIDDSGFSLLQQTLQRLSGLDGVEVAAPLV
CCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHCCEE
ICNENHRFLVAEQLREIDQLATNIILEPIGRNTAPAVALAAHLAAEEDDDSILLVLAADH
EECCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEHHH
LIKKVENFHAAIKTAIMHAAEDNLVTFGIIPGHPETGYGYIKRGTALSSQCFKVDAFVEK
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEECCCCCCCHHEEEHHHHHH
PCLEKATEYLASGEFSWNSGMFMFKTAKFLQELQQFSPEIFSTTQQSVVDSQRDMNFIRV
HHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEE
DQEIFKHCPSDSIDYAVMEKTRDAVVIPIDAGWSDVGSWTSLWDVSEKDALGNVNVGEII
CHHHHHHCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCEE
SIDSNNNYISSDGALVATIGLDDLIVINTNDALLISTKDRVQDVKKVVDELKKRNLHHFR
EECCCCCEEECCCCEEEEECCCCEEEEECCCEEEEECHHHHHHHHHHHHHHHHCCHHHHH
QHSSAYRPWGKICDIDNGEHFQVKKIIVRPGEGLSVQRHLHRAEHWVVVSGTAKVNVEDK
HCCCCCCCCCCEECCCCCCCEEEEEEEEECCCCCHHHHHHHHCCEEEEEECCEEEECCHH
EFFLSENQSTFIPAGSVHTLENPGKIDLEIIEVRSGFYLEEDDIERLHDRYGRV
HHEECCCCCEEECCCCEEECCCCCCEEEEEEEECCCCEECHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKLIPVVMAGGTGSRLWPLSRESFPKQFLSIDDSGFSLLQQTLQRLSGLDGVEVAAPLV
CCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHCCEE
ICNENHRFLVAEQLREIDQLATNIILEPIGRNTAPAVALAAHLAAEEDDDSILLVLAADH
EECCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEHHH
LIKKVENFHAAIKTAIMHAAEDNLVTFGIIPGHPETGYGYIKRGTALSSQCFKVDAFVEK
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEECCCCCCCHHEEEHHHHHH
PCLEKATEYLASGEFSWNSGMFMFKTAKFLQELQQFSPEIFSTTQQSVVDSQRDMNFIRV
HHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEE
DQEIFKHCPSDSIDYAVMEKTRDAVVIPIDAGWSDVGSWTSLWDVSEKDALGNVNVGEII
CHHHHHHCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCEE
SIDSNNNYISSDGALVATIGLDDLIVINTNDALLISTKDRVQDVKKVVDELKKRNLHHFR
EECCCCCEEECCCCEEEEECCCCEEEEECCCEEEEECHHHHHHHHHHHHHHHHCCHHHHH
QHSSAYRPWGKICDIDNGEHFQVKKIIVRPGEGLSVQRHLHRAEHWVVVSGTAKVNVEDK
HCCCCCCCCCCEECCCCCCCEEEEEEEEECCCCCHHHHHHHHCCEEEEEECCEEEECCHH
EFFLSENQSTFIPAGSVHTLENPGKIDLEIIEVRSGFYLEEDDIERLHDRYGRV
HHEECCCCCEEECCCCEEECCCCCCEEEEEEEECCCCEECHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379320 [H]