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The map label for this gene is emrA [H]

Identifier: 157369575

GI number: 157369575

Start: 1462704

End: 1463732

Strand: Reverse

Name: emrA [H]

Synonym: Spro_1332

Alternate gene names: 157369575

Gene position: 1463732-1462704 (Counterclockwise)

Preceding gene: 157369576

Following gene: 157369573

Centisome position: 26.86

GC content: 61.32

Gene sequence:

>1029_bases
ATGAGCTTCACCCCTGCAAAACGAACCTTAACCCTGGCGTTGCTGCTGTTAATCCTGCTGGCGATTGCTTTTTTTGTCCG
CTCATCGCTCAATCGCCACCATTTACGTACCGATGATGCGGTCGTGAGCGCCGATTACACGCTGGTGGCCCCCAAGATTT
CCGGCTATATCCGCAGCGTCAACGTCAGTGACAACCAGCAGGTAAAAGCCGGTGAAGTTCTGGCCACCCTCGACGATCGG
GATTACCGCGTGGCGCTGGAAACGGCGCAGGCCAATCTGCAGATTAGCCAGGCCAAATTGTCCAGTATTCAGGCGCAGCT
GGATCAACAGCAGGCCAATATTGCCCAGCAGGAAGCCGGCGTCAGCGCCAGCCAGGCCACGCTCAACTACGCCGGGCAGA
ACGCCGATCGCTATCGCCGGTTGCTGAAAACCGGTACCGCCACCGCTGATGAGCAGCAGAAATCGGCCTCCGTGATGCAG
ACCGCCGCCGCACAGCTAAAGCAGAATCAGGCGGCGGTGGTTTCGGCACGTAAAGAAGTCGGGGTGTTGCAGGCCAGCCG
CAAACAGGCCGAAGCGAATATTGCCGCCTCGCAGGCCAGCGTTGATCAGGCGCGTCTGAACCTTTCCTATACCCAGATTA
TTGCGCCGATTGCCGGCAGCGTCGGCCAGCGCGCGGTACGCCAGGGTGCCTGGGTCTCCGCCGGTACGCGTCTGCTGGCG
GTGGTGCCGCTACAACAGAGCTACGTGGTCGCCAACTTCCTGGAAACCCAGTTAGCCAACGTGTCTGCCGGCCAGCCGGT
ATCCATCACCGTCGATGCGCTGCCCGGCGTGACGCTGCGCGGCCACGTTGACAGCATCGCCCCGGCCACTGGCTCGACCT
TTGCCGCCATCAGCGCCGATAACGCCACCGGTAACTACACCAAAGTGGTGCAACGCCTGCCGGTGAAGATCCTGCTGGAA
CCCAACCAGCCGGAACTGGCGCGACTGCGCGTTGGCATGTCGGTGGTGCCGGAACTTGAGATGCGCTGA

Upstream 100 bases:

>100_bases
GTGCGCTGCTTTTGGTGTTGCTGACCGCCTGGTTGCCGAAGCGTGTCTATCCGCCGCAAACGCTCTTACCTCTAGTTCCA
AAAACATCAGGATCATAACC

Downstream 100 bases:

>100_bases
TTTTTACGTATAAAAAAGGCCACCCTAAAGGTGGCCTCAGACGGCTGACAAAGTCATTTGTAGCTAAAAGTACTGTTTTA
GGTACTAACAAAAACAAAGG

Product: secretion protein HlyD family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MSFTPAKRTLTLALLLLILLAIAFFVRSSLNRHHLRTDDAVVSADYTLVAPKISGYIRSVNVSDNQQVKAGEVLATLDDR
DYRVALETAQANLQISQAKLSSIQAQLDQQQANIAQQEAGVSASQATLNYAGQNADRYRRLLKTGTATADEQQKSASVMQ
TAAAQLKQNQAAVVSARKEVGVLQASRKQAEANIAASQASVDQARLNLSYTQIIAPIAGSVGQRAVRQGAWVSAGTRLLA
VVPLQQSYVVANFLETQLANVSAGQPVSITVDALPGVTLRGHVDSIAPATGSTFAAISADNATGNYTKVVQRLPVKILLE
PNQPELARLRVGMSVVPELEMR

Sequences:

>Translated_342_residues
MSFTPAKRTLTLALLLLILLAIAFFVRSSLNRHHLRTDDAVVSADYTLVAPKISGYIRSVNVSDNQQVKAGEVLATLDDR
DYRVALETAQANLQISQAKLSSIQAQLDQQQANIAQQEAGVSASQATLNYAGQNADRYRRLLKTGTATADEQQKSASVMQ
TAAAQLKQNQAAVVSARKEVGVLQASRKQAEANIAASQASVDQARLNLSYTQIIAPIAGSVGQRAVRQGAWVSAGTRLLA
VVPLQQSYVVANFLETQLANVSAGQPVSITVDALPGVTLRGHVDSIAPATGSTFAAISADNATGNYTKVVQRLPVKILLE
PNQPELARLRVGMSVVPELEMR
>Mature_341_residues
SFTPAKRTLTLALLLLILLAIAFFVRSSLNRHHLRTDDAVVSADYTLVAPKISGYIRSVNVSDNQQVKAGEVLATLDDRD
YRVALETAQANLQISQAKLSSIQAQLDQQQANIAQQEAGVSASQATLNYAGQNADRYRRLLKTGTATADEQQKSASVMQT
AAAQLKQNQAAVVSARKEVGVLQASRKQAEANIAASQASVDQARLNLSYTQIIAPIAGSVGQRAVRQGAWVSAGTRLLAV
VPLQQSYVVANFLETQLANVSAGQPVSITVDALPGVTLRGHVDSIAPATGSTFAAISADNATGNYTKVVQRLPVKILLEP
NQPELARLRVGMSVVPELEMR

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=342, Percent_Identity=36.8421052631579, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1788711, Length=332, Percent_Identity=29.8192771084337, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1790519, Length=320, Percent_Identity=32.8125, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1789637, Length=321, Percent_Identity=30.8411214953271, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87081951, Length=312, Percent_Identity=26.9230769230769, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790024, Length=272, Percent_Identity=26.4705882352941, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1790012, Length=275, Percent_Identity=26.5454545454545, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787013, Length=237, Percent_Identity=29.535864978903, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789900, Length=320, Percent_Identity=24.375, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 36479; Mature: 36348

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFTPAKRTLTLALLLLILLAIAFFVRSSLNRHHLRTDDAVVSADYTLVAPKISGYIRSV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCEEEECCCCCCEEEEE
NVSDNQQVKAGEVLATLDDRDYRVALETAQANLQISQAKLSSIQAQLDQQQANIAQQEAG
CCCCCCCCCCCCEEEEECCCCEEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCC
VSASQATLNYAGQNADRYRRLLKTGTATADEQQKSASVMQTAAAQLKQNQAAVVSARKEV
CCHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
GVLQASRKQAEANIAASQASVDQARLNLSYTQIIAPIAGSVGQRAVRQGAWVSAGTRLLA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
VVPLQQSYVVANFLETQLANVSAGQPVSITVDALPGVTLRGHVDSIAPATGSTFAAISAD
EEECCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCEEEEEECC
NATGNYTKVVQRLPVKILLEPNQPELARLRVGMSVVPELEMR
CCCCHHHHHHHHCCEEEEECCCCCHHHHHHHCHHHCCCCCCC
>Mature Secondary Structure 
SFTPAKRTLTLALLLLILLAIAFFVRSSLNRHHLRTDDAVVSADYTLVAPKISGYIRSV
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCEEEECCCCCCEEEEE
NVSDNQQVKAGEVLATLDDRDYRVALETAQANLQISQAKLSSIQAQLDQQQANIAQQEAG
CCCCCCCCCCCCEEEEECCCCEEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCC
VSASQATLNYAGQNADRYRRLLKTGTATADEQQKSASVMQTAAAQLKQNQAAVVSARKEV
CCHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
GVLQASRKQAEANIAASQASVDQARLNLSYTQIIAPIAGSVGQRAVRQGAWVSAGTRLLA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
VVPLQQSYVVANFLETQLANVSAGQPVSITVDALPGVTLRGHVDSIAPATGSTFAAISAD
EEECCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCEEEEEECC
NATGNYTKVVQRLPVKILLEPNQPELARLRVGMSVVPELEMR
CCCCHHHHHHHHCCEEEEECCCCCHHHHHHHCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]