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The map label for this gene is cysN [H]

Identifier: 157369064

GI number: 157369064

Start: 914606

End: 916051

Strand: Direct

Name: cysN [H]

Synonym: Spro_0819

Alternate gene names: 157369064

Gene position: 914606-916051 (Clockwise)

Preceding gene: 157369063

Following gene: 157369065

Centisome position: 16.79

GC content: 56.15

Gene sequence:

>1446_bases
ATGAACACTATAGTTTCGCAAAACCAGGCAATTGCCCAACAAATCGCTGACCAGGGCGGGGTCGAGTCTTACCTGCACGC
GCAGCAACATAAGAGTCTGTTACGGTTCCTGACCTGCGGCAGCGTCGACGACGGTAAAAGTACCCTGATCGGTCGTCTGC
TGCACGATACCCGCCAGATCTACGAAGATCAGCTGTCCACGCTGCACAGTGACAGCAAGCGTATCGGAACCCAGGGCGAA
AAGCTCGATCTGGCATTGCTGGTCGACGGCCTGCAGGCCGAGCGCGAGCAGGGCATCACCATTGACGTGGCCTATCGCTA
TTTCTCAACTGAAAAGCGCAAATTTATTATCGCCGATACCCCTGGGCATGAGCAGTACACCCGCAATATGGCGACCGGTG
CCTCCACCTGCGATCTGGCAATTTTGCTGATTGACGCGCGTAAAGGGGTGCTGGATCAAACCCGCAGACACAGTTTTATT
GCTACGTTGCTGGGCATTCGCCATTTGGTGGTGGCGGTCAACAAGATGGATCTGGTGGATTACCAAGAGTCGGTATTTGA
GCAGTTCAAACAGGATTATCTGACCTTCGCTCAGCAGTTGCCAAAGGATCTGGATATCAAGTTTGTCCCGCTGTCGGCAC
TGGACGGCGATAACGTGGCCGGCGAAAGCACCCATATGCCTTGGTACAACGGCCCTACGTTGTTGCAGGTATTGGAAAGT
GTGGATGTTGTCAGCGAGCGTGAAACGCAACCGCTGCGCTTCCCGGTGCAATATGTTAACCGTCCGAACCTGGATTTTCG
TGGTTATGCAGGCACCCTGTCGGCCGGTGTCGTGCGCGTGGGTCAACGTGTGAAGGTGTTGCCTTCCGGTGTGGAATCCA
CCGTTGCACGTATTGTCACCTTTGATGGTGATTTGCAGGAAGCGGTACCGGGTGAAGCGATCACCCTGGTGCTGAAGGAT
GAAGTGGATATCAGCCGCGGCGATTTGCTGGTTGATGCCGGGGAAACCCTGCAGGCGGCGCAGAGCGCGCTGGTGGACGT
GGTATGGATGGCCGAGAAGCCGCTGTTACCGGGGCAGAGCTACGACATCAAGGTCGCTGGCAAAAAGACTCGCGCACGAG
TGGAAGGCATTCGCCACCAGGTAGAAATCAACTCGCTGGCTCAGCATCAGGCGGATACCTTGCCGCTGAACGGCATTGGC
CTGGTGGAACTGACCTTCGACGAACCGCTGGTGCTGGACAGCTATCAGCGTAATCATGACACCGGCGGATTTATCTTTAT
CGACCGCCTGAGCAATGTCACGGTTGGTGCCGGGCTGATCCGCGAAACCTTGCAGGACGCTCAGGCAGCCACTGGTGACT
TCAGCGCATTCGAGCTGGAACTGAATGCTTTGGTGCGTAAACACTTCCCGCATTGGGGCGCACGCGACCTGATCGGGGGC
AAGTAA

Upstream 100 bases:

>100_bases
TATCCACCACCAGTGAGCGCCAGGGACGGATGATCGATCGCGATCAGTCCGGCTCGATGGAGCTGAAAAAGCGTCAAGGG
TATTTCTAAGGAGCCGCCGG

Downstream 100 bases:

>100_bases
CGTGGCCGACGTAGAGCAACGACCCACCGGGCCGGATGATGACAACGTGGTCTGGCATCCGCATGCGGTAGCGCGTGCGG
ATCGCGAAGCCCGTAATGGC

Product: sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MNTIVSQNQAIAQQIADQGGVESYLHAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSTLHSDSKRIGTQGE
KLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCDLAILLIDARKGVLDQTRRHSFI
ATLLGIRHLVVAVNKMDLVDYQESVFEQFKQDYLTFAQQLPKDLDIKFVPLSALDGDNVAGESTHMPWYNGPTLLQVLES
VDVVSERETQPLRFPVQYVNRPNLDFRGYAGTLSAGVVRVGQRVKVLPSGVESTVARIVTFDGDLQEAVPGEAITLVLKD
EVDISRGDLLVDAGETLQAAQSALVDVVWMAEKPLLPGQSYDIKVAGKKTRARVEGIRHQVEINSLAQHQADTLPLNGIG
LVELTFDEPLVLDSYQRNHDTGGFIFIDRLSNVTVGAGLIRETLQDAQAATGDFSAFELELNALVRKHFPHWGARDLIGG
K

Sequences:

>Translated_481_residues
MNTIVSQNQAIAQQIADQGGVESYLHAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSTLHSDSKRIGTQGE
KLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCDLAILLIDARKGVLDQTRRHSFI
ATLLGIRHLVVAVNKMDLVDYQESVFEQFKQDYLTFAQQLPKDLDIKFVPLSALDGDNVAGESTHMPWYNGPTLLQVLES
VDVVSERETQPLRFPVQYVNRPNLDFRGYAGTLSAGVVRVGQRVKVLPSGVESTVARIVTFDGDLQEAVPGEAITLVLKD
EVDISRGDLLVDAGETLQAAQSALVDVVWMAEKPLLPGQSYDIKVAGKKTRARVEGIRHQVEINSLAQHQADTLPLNGIG
LVELTFDEPLVLDSYQRNHDTGGFIFIDRLSNVTVGAGLIRETLQDAQAATGDFSAFELELNALVRKHFPHWGARDLIGG
K
>Mature_481_residues
MNTIVSQNQAIAQQIADQGGVESYLHAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSTLHSDSKRIGTQGE
KLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCDLAILLIDARKGVLDQTRRHSFI
ATLLGIRHLVVAVNKMDLVDYQESVFEQFKQDYLTFAQQLPKDLDIKFVPLSALDGDNVAGESTHMPWYNGPTLLQVLES
VDVVSERETQPLRFPVQYVNRPNLDFRGYAGTLSAGVVRVGQRVKVLPSGVESTVARIVTFDGDLQEAVPGEAITLVLKD
EVDISRGDLLVDAGETLQAAQSALVDVVWMAEKPLLPGQSYDIKVAGKKTRARVEGIRHQVEINSLAQHQADTLPLNGIG
LVELTFDEPLVLDSYQRNHDTGGFIFIDRLSNVTVGAGLIRETLQDAQAATGDFSAFELELNALVRKHFPHWGARDLIGG
K

Specific function: May be the GTPase, regulating ATP sulfurylase activity [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503475, Length=461, Percent_Identity=26.2472885032538, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI194018520, Length=336, Percent_Identity=31.547619047619, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI4503471, Length=329, Percent_Identity=30.6990881458967, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI194097354, Length=336, Percent_Identity=31.547619047619, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI194018522, Length=336, Percent_Identity=31.547619047619, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI46094014, Length=336, Percent_Identity=31.547619047619, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI223555963, Length=457, Percent_Identity=27.5711159737418, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI5729864, Length=457, Percent_Identity=27.5711159737418, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI34147630, Length=437, Percent_Identity=27.6887871853547, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1789108, Length=474, Percent_Identity=79.957805907173, Blast_Score=788, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=436, Percent_Identity=25.2293577981651, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790412, Length=437, Percent_Identity=25.1716247139588, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI2367247, Length=94, Percent_Identity=39.3617021276596, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17552884, Length=444, Percent_Identity=28.8288288288288, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17569207, Length=444, Percent_Identity=28.8288288288288, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI32566629, Length=435, Percent_Identity=27.5862068965517, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI115532067, Length=314, Percent_Identity=30.5732484076433, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI115532065, Length=314, Percent_Identity=30.5732484076433, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI32566303, Length=437, Percent_Identity=27.4599542334096, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI25141371, Length=238, Percent_Identity=26.0504201680672, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI32566301, Length=138, Percent_Identity=36.9565217391304, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17556456, Length=343, Percent_Identity=27.1137026239067, Blast_Score=85, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6325337, Length=329, Percent_Identity=33.1306990881459, Blast_Score=177, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6319594, Length=329, Percent_Identity=33.1306990881459, Blast_Score=177, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6322937, Length=374, Percent_Identity=30.7486631016043, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6320377, Length=426, Percent_Identity=26.7605633802817, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6324761, Length=333, Percent_Identity=27.3273273273273, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24652838, Length=436, Percent_Identity=27.0642201834862, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI17137572, Length=436, Percent_Identity=27.0642201834862, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI17137380, Length=335, Percent_Identity=29.8507462686567, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI45553807, Length=436, Percent_Identity=25.9174311926606, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI45553816, Length=436, Percent_Identity=25.9174311926606, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24651721, Length=436, Percent_Identity=25.9174311926606, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI17864154, Length=436, Percent_Identity=25.9174311926606, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI45550900, Length=432, Percent_Identity=26.6203703703704, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI281363316, Length=425, Percent_Identity=26.8235294117647, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI17864358, Length=425, Percent_Identity=26.8235294117647, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI19921738, Length=308, Percent_Identity=26.9480519480519, Blast_Score=83, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 53112; Mature: 53112

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTIVSQNQAIAQQIADQGGVESYLHAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQI
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
YEDQLSTLHSDSKRIGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADT
HHHHHHHHHCCHHHCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEEEC
PGHEQYTRNMATGASTCDLAILLIDARKGVLDQTRRHSFIATLLGIRHLVVAVNKMDLVD
CCCHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YQESVFEQFKQDYLTFAQQLPKDLDIKFVPLSALDGDNVAGESTHMPWYNGPTLLQVLES
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
VDVVSERETQPLRFPVQYVNRPNLDFRGYAGTLSAGVVRVGQRVKVLPSGVESTVARIVT
HHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCHHHHHHHHEE
FDGDLQEAVPGEAITLVLKDEVDISRGDLLVDAGETLQAAQSALVDVVWMAEKPLLPGQS
ECCCHHHHCCCCEEEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCC
YDIKVAGKKTRARVEGIRHQVEINSLAQHQADTLPLNGIGLVELTFDEPLVLDSYQRNHD
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEECCHHCCCC
TGGFIFIDRLSNVTVGAGLIRETLQDAQAATGDFSAFELELNALVRKHFPHWGARDLIGG
CCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCC
K
C
>Mature Secondary Structure
MNTIVSQNQAIAQQIADQGGVESYLHAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQI
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
YEDQLSTLHSDSKRIGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADT
HHHHHHHHHCCHHHCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEEEC
PGHEQYTRNMATGASTCDLAILLIDARKGVLDQTRRHSFIATLLGIRHLVVAVNKMDLVD
CCCHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YQESVFEQFKQDYLTFAQQLPKDLDIKFVPLSALDGDNVAGESTHMPWYNGPTLLQVLES
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
VDVVSERETQPLRFPVQYVNRPNLDFRGYAGTLSAGVVRVGQRVKVLPSGVESTVARIVT
HHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCHHHHHHHHEE
FDGDLQEAVPGEAITLVLKDEVDISRGDLLVDAGETLQAAQSALVDVVWMAEKPLLPGQS
ECCCHHHHCCCCEEEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCC
YDIKVAGKKTRARVEGIRHQVEINSLAQHQADTLPLNGIGLVELTFDEPLVLDSYQRNHD
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEECCHHCCCC
TGGFIFIDRLSNVTVGAGLIRETLQDAQAATGDFSAFELELNALVRKHFPHWGARDLIGG
CCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA