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The map label for this gene is cysD [H]

Identifier: 157369063

GI number: 157369063

Start: 913686

End: 914594

Strand: Direct

Name: cysD [H]

Synonym: Spro_0818

Alternate gene names: 157369063

Gene position: 913686-914594 (Clockwise)

Preceding gene: 157369062

Following gene: 157369064

Centisome position: 16.77

GC content: 56.0

Gene sequence:

>909_bases
ATGGACGAAAAACGACTCACTCATTTGCGGCAATTGGAGGCGGAGAGTATCCATATCATCCGTGAAGTCGCCGCTGAATT
CGGCAACCCGGTGATGCTCTATTCCATCGGCAAAGACTCTTCCGTGATGCTGCACCTGGCGCGCAAGGCCTTCTTCCCAG
GCACGCTGCCGTTTCCGCTGCTACACGTGGATACCGGCTGGAAATTCAGCGAGATGTACCAGTTCCGTGACCGCACCGCC
AAGGAATACGGTTTTGAGCTGCTGGTACATAAAAATCCGGAAGGCGTGGCGATGGGCATCAACCCGTTCGTTCACGGCAG
TGCCAAGCACACCGATATCATGAAAACCGAAGGTCTGAAGCAGGCGCTGAACAAATACGGTTTTGATGCCGCCTTTGGTG
GCGCTCGCCGTGACGAAGAAAAATCACGTGCCAAAGAGCGTATCTATTCTTTCCGCGACCGCTTCCACCGTTGGGATCCG
AAGAACCAGCGCCCGGAGCTGTGGCACAACTACAACGGCCAGATCAACAAGGGCGAGAGCATCCGCGTGTTCCCGCTGTC
GAACTGGACCGAGCTGGATATCTGGCAATACATTTTCCTGGAAAAAATCGACATCATTCCTTTGTACCTGGCGAAACCAC
GTCCGGTGGTAGAGCGTGACGGCATGCTGTTGATGGTGGACGACGATCGCATTGATCTGCAGCCGGGCGAAGTGATCAGC
CAGCGCATGGTGCGCTTCCGCACCCTGGGTTGCTGGCCGCTGACCGGCGCGGTGGAGTCCGAAGCGCAAACGCTGCCGGA
AATCATCGAAGAGATGTTGGTATCCACCACCAGTGAGCGCCAGGGACGGATGATCGATCGCGATCAGTCCGGCTCGATGG
AGCTGAAAAAGCGTCAAGGGTATTTCTAA

Upstream 100 bases:

>100_bases
AGTGGTGGAATTACATCACCAAATCGCCTGGTTTGGGCATCAACCGCAGGCGGAAGGGGTATCACGCCCAGCCGTCGTGA
ATTTGGCTTAAGGATCTGTT

Downstream 100 bases:

>100_bases
GGAGCCGCCGGATGAACACTATAGTTTCGCAAAACCAGGCAATTGCCCAACAAATCGCTGACCAGGGCGGGGTCGAGTCT
TACCTGCACGCGCAGCAACA

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MDEKRLTHLRQLEAESIHIIREVAAEFGNPVMLYSIGKDSSVMLHLARKAFFPGTLPFPLLHVDTGWKFSEMYQFRDRTA
KEYGFELLVHKNPEGVAMGINPFVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
KNQRPELWHNYNGQINKGESIRVFPLSNWTELDIWQYIFLEKIDIIPLYLAKPRPVVERDGMLLMVDDDRIDLQPGEVIS
QRMVRFRTLGCWPLTGAVESEAQTLPEIIEEMLVSTTSERQGRMIDRDQSGSMELKKRQGYF

Sequences:

>Translated_302_residues
MDEKRLTHLRQLEAESIHIIREVAAEFGNPVMLYSIGKDSSVMLHLARKAFFPGTLPFPLLHVDTGWKFSEMYQFRDRTA
KEYGFELLVHKNPEGVAMGINPFVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
KNQRPELWHNYNGQINKGESIRVFPLSNWTELDIWQYIFLEKIDIIPLYLAKPRPVVERDGMLLMVDDDRIDLQPGEVIS
QRMVRFRTLGCWPLTGAVESEAQTLPEIIEEMLVSTTSERQGRMIDRDQSGSMELKKRQGYF
>Mature_302_residues
MDEKRLTHLRQLEAESIHIIREVAAEFGNPVMLYSIGKDSSVMLHLARKAFFPGTLPFPLLHVDTGWKFSEMYQFRDRTA
KEYGFELLVHKNPEGVAMGINPFVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
KNQRPELWHNYNGQINKGESIRVFPLSNWTELDIWQYIFLEKIDIIPLYLAKPRPVVERDGMLLMVDDDRIDLQPGEVIS
QRMVRFRTLGCWPLTGAVESEAQTLPEIIEEMLVSTTSERQGRMIDRDQSGSMELKKRQGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=302, Percent_Identity=92.3841059602649, Blast_Score=589, Evalue=1e-170,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 35167; Mature: 35167

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEKRLTHLRQLEAESIHIIREVAAEFGNPVMLYSIGKDSSVMLHLARKAFFPGTLPFPL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCE
LHVDTGWKFSEMYQFRDRTAKEYGFELLVHKNPEGVAMGINPFVHGSAKHTDIMKTEGLK
EEECCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHH
QALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDPKNQRPELWHNYNGQINKGES
HHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCE
IRVFPLSNWTELDIWQYIFLEKIDIIPLYLAKPRPVVERDGMLLMVDDDRIDLQPGEVIS
EEEEECCCCCCHHHHHHHHHHHHCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCHHHHH
QRMVRFRTLGCWPLTGAVESEAQTLPEIIEEMLVSTTSERQGRMIDRDQSGSMELKKRQG
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCHHHHCCC
YF
CC
>Mature Secondary Structure
MDEKRLTHLRQLEAESIHIIREVAAEFGNPVMLYSIGKDSSVMLHLARKAFFPGTLPFPL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCE
LHVDTGWKFSEMYQFRDRTAKEYGFELLVHKNPEGVAMGINPFVHGSAKHTDIMKTEGLK
EEECCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHH
QALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDPKNQRPELWHNYNGQINKGES
HHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCE
IRVFPLSNWTELDIWQYIFLEKIDIIPLYLAKPRPVVERDGMLLMVDDDRIDLQPGEVIS
EEEEECCCCCCHHHHHHHHHHHHCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCHHHHH
QRMVRFRTLGCWPLTGAVESEAQTLPEIIEEMLVSTTSERQGRMIDRDQSGSMELKKRQG
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCHHHHCCC
YF
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA