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The map label for this gene is cysG1

Identifier: 157369062

GI number: 157369062

Start: 912246

End: 913676

Strand: Direct

Name: cysG1

Synonym: Spro_0817

Alternate gene names: 157369062

Gene position: 912246-913676 (Clockwise)

Preceding gene: 157369061

Following gene: 157369063

Centisome position: 16.74

GC content: 61.36

Gene sequence:

>1431_bases
GTGGACTATCTACCTATATTCGCCGACCTGAAACAACGCCCGGTGTTGGTCGTTGGCGGCGGTGAAGTGGCTGCGCGCAA
AGTTGACCTTCTCCTGCGCGCCGGGGCTGAAATACGGATAGTTGCGCAGTCACTCTCACCGATACTCGAACAACTGAGTC
AGCAGGGGCAGATCCACTGGCTGGGACAGGCTTTTGCCGCTGAGCAATTGGATGAGGTTTTCCTGGTGATTGCCGCCACC
GATGACAGTGCGCTGAATGCCGAGGTATTTAGCGAGGCGGATAAACGCCGGGTGCTGGCCAACGTAGTGGACGATCAGCC
GCGCTGTTCGTTCATCTTCCCGTCGATTATCGACCGTTCACCCTTGGTGGTCGCAGTCTCTTCCAGCGGTCAGGCACCGG
TGCTGGCACGCATGCTGCGTGAAAAACTTGAGGCCTTGTTGCCCGCCAGCCTGGGACAGATGGCTGAGGTAGCCGGGCGC
TGGCGTGGTCAGGTGAAGCAACGGTTGAATGCCATTGGCGAACGCCGCCGCTTTTGGGAGAAAACCTTTGGCGGCCGCTT
CGCAACGCTGGTGGCCAATGGCCAAACCGCCGAGGCGCAGCGGCAACTGGAGCAGGACTTAGAGCAGTTTGCACAGGGCA
GCGAAGGCACTCAGGGCGAAATTGCCCTGGTGGGGGCCGGGCCGGGCGACGTGGGGTTACTGACCCTGCGCGGGCTGCAG
GTGATGCAGCAGGCCGACGTGGTGCTGTACGACCATCTGGTTAGCGACGAAATTCTTGATTTGGTTCGTCGGGATGCCGA
ACGCATCTGCGTGGGCAAACGCGCCGGTGCACACTCGGTGATCCAGGAAGAAACCAACCGTCTGCTGGTGGAACTGGCAC
AGCAGGGCAAACGTGTGGTGCGGCTGAAGGGTGGCGATCCCTTTATCTTTGGCCGTGGTGGTGAAGAATTACAGGTGGCC
GCCGCGGCGGGCATTCCCTTCCAGGTGGTACCGGGAGTAACGGCCGCCGCCGGGGCAACCGCCTATGCCGGCATCCCGCT
GACGCACCGCGATCACGCACAGAGCGTTACCTTTATTACTGGCCACTGTCGCCCCGACGGCGATGGCCTGGACTGGGCCG
ATTTGGCGCGTGCGCGCCAGACTCTGGCTATCTACATGGGCACCATGAAGGCGGCGGATATCAGCCAGCGCCTGATAGCC
CACGGCCGTGCGGCCACAACGCCGGTGGCGGTAATCAGCCGTGGCACCCGTGCGGACCAACTGGTGCAAACCGGTACGCT
GCAACAACTCGAACAATTGGCTCAACAGGCACCGTTACCGGCGCTGCTGGTGATCGGCGAAGTGGTGGAATTACATCACC
AAATCGCCTGGTTTGGGCATCAACCGCAGGCGGAAGGGGTATCACGCCCAGCCGTCGTGAATTTGGCTTAA

Upstream 100 bases:

>100_bases
ATATTCCATAAAAGAACTAGTCATTCCTTTTCGTCATTTCAGAGGCTAAATCTCAGCCCGTATAGTCACAGTATCTTTCT
GCATATATGAAAAGGCTGTT

Downstream 100 bases:

>100_bases
GGATCTGTTATGGACGAAAAACGACTCACTCATTTGCGGCAATTGGAGGCGGAGAGTATCCATATCATCCGTGAAGTCGC
CGCTGAATTCGGCAACCCGG

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MDYLPIFADLKQRPVLVVGGGEVAARKVDLLLRAGAEIRIVAQSLSPILEQLSQQGQIHWLGQAFAAEQLDEVFLVIAAT
DDSALNAEVFSEADKRRVLANVVDDQPRCSFIFPSIIDRSPLVVAVSSSGQAPVLARMLREKLEALLPASLGQMAEVAGR
WRGQVKQRLNAIGERRRFWEKTFGGRFATLVANGQTAEAQRQLEQDLEQFAQGSEGTQGEIALVGAGPGDVGLLTLRGLQ
VMQQADVVLYDHLVSDEILDLVRRDAERICVGKRAGAHSVIQEETNRLLVELAQQGKRVVRLKGGDPFIFGRGGEELQVA
AAAGIPFQVVPGVTAAAGATAYAGIPLTHRDHAQSVTFITGHCRPDGDGLDWADLARARQTLAIYMGTMKAADISQRLIA
HGRAATTPVAVISRGTRADQLVQTGTLQQLEQLAQQAPLPALLVIGEVVELHHQIAWFGHQPQAEGVSRPAVVNLA

Sequences:

>Translated_476_residues
MDYLPIFADLKQRPVLVVGGGEVAARKVDLLLRAGAEIRIVAQSLSPILEQLSQQGQIHWLGQAFAAEQLDEVFLVIAAT
DDSALNAEVFSEADKRRVLANVVDDQPRCSFIFPSIIDRSPLVVAVSSSGQAPVLARMLREKLEALLPASLGQMAEVAGR
WRGQVKQRLNAIGERRRFWEKTFGGRFATLVANGQTAEAQRQLEQDLEQFAQGSEGTQGEIALVGAGPGDVGLLTLRGLQ
VMQQADVVLYDHLVSDEILDLVRRDAERICVGKRAGAHSVIQEETNRLLVELAQQGKRVVRLKGGDPFIFGRGGEELQVA
AAAGIPFQVVPGVTAAAGATAYAGIPLTHRDHAQSVTFITGHCRPDGDGLDWADLARARQTLAIYMGTMKAADISQRLIA
HGRAATTPVAVISRGTRADQLVQTGTLQQLEQLAQQAPLPALLVIGEVVELHHQIAWFGHQPQAEGVSRPAVVNLA
>Mature_476_residues
MDYLPIFADLKQRPVLVVGGGEVAARKVDLLLRAGAEIRIVAQSLSPILEQLSQQGQIHWLGQAFAAEQLDEVFLVIAAT
DDSALNAEVFSEADKRRVLANVVDDQPRCSFIFPSIIDRSPLVVAVSSSGQAPVLARMLREKLEALLPASLGQMAEVAGR
WRGQVKQRLNAIGERRRFWEKTFGGRFATLVANGQTAEAQRQLEQDLEQFAQGSEGTQGEIALVGAGPGDVGLLTLRGLQ
VMQQADVVLYDHLVSDEILDLVRRDAERICVGKRAGAHSVIQEETNRLLVELAQQGKRVVRLKGGDPFIFGRGGEELQVA
AAAGIPFQVVPGVTAAAGATAYAGIPLTHRDHAQSVTFITGHCRPDGDGLDWADLARARQTLAIYMGTMKAADISQRLIA
HGRAATTPVAVISRGTRADQLVQTGTLQQLEQLAQQAPLPALLVIGEVVELHHQIAWFGHQPQAEGVSRPAVVNLA

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family

Homologues:

Organism=Escherichia coli, GI1789768, Length=462, Percent_Identity=55.8441558441558, Blast_Score=493, Evalue=1e-140,
Organism=Saccharomyces cerevisiae, GI6322922, Length=234, Percent_Identity=35.4700854700855, Blast_Score=127, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSG1_SERP5 (A8G9Y3)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477051.1
- ProteinModelPortal:   A8G9Y3
- SMR:   A8G9Y3
- STRING:   A8G9Y3
- GeneID:   5603444
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0817
- eggNOG:   COG0007
- HOGENOM:   HBG730212
- OMA:   PVLAQMI
- ProtClustDB:   CLSK889477
- BioCyc:   SPRO399741:SPRO_0817-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01646
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043
- Gene3D:   G3DSA:3.40.1010.10
- Gene3D:   G3DSA:3.30.950.10
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.8.210
- TIGRFAMs:   TIGR01469
- TIGRFAMs:   TIGR01470

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4

Molecular weight: Translated: 51267; Mature: 51267

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00839 SUMT_1; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYLPIFADLKQRPVLVVGGGEVAARKVDLLLRAGAEIRIVAQSLSPILEQLSQQGQIHW
CCCCCCHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCEEE
LGQAFAAEQLDEVFLVIAATDDSALNAEVFSEADKRRVLANVVDDQPRCSFIFPSIIDRS
HHHHHHHHHHCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECHHHCCCC
PLVVAVSSSGQAPVLARMLREKLEALLPASLGQMAEVAGRWRGQVKQRLNAIGERRRFWE
CEEEEECCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTFGGRFATLVANGQTAEAQRQLEQDLEQFAQGSEGTQGEIALVGAGPGDVGLLTLRGLQ
HHCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEHHHHHHHH
VMQQADVVLYDHLVSDEILDLVRRDAERICVGKRAGAHSVIQEETNRLLVELAQQGKRVV
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEE
RLKGGDPFIFGRGGEELQVAAAAGIPFQVVPGVTAAAGATAYAGIPLTHRDHAQSVTFIT
EEECCCEEEECCCCCEEEEEEECCCCEEECCCCHHHCCCCCCCCCCCCCCCCCCEEEEEE
GHCRPDGDGLDWADLARARQTLAIYMGTMKAADISQRLIAHGRAATTPVAVISRGTRADQ
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHH
LVQTGTLQQLEQLAQQAPLPALLVIGEVVELHHQIAWFGHQPQAEGVSRPAVVNLA
HHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECC
>Mature Secondary Structure
MDYLPIFADLKQRPVLVVGGGEVAARKVDLLLRAGAEIRIVAQSLSPILEQLSQQGQIHW
CCCCCCHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCEEE
LGQAFAAEQLDEVFLVIAATDDSALNAEVFSEADKRRVLANVVDDQPRCSFIFPSIIDRS
HHHHHHHHHHCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECHHHCCCC
PLVVAVSSSGQAPVLARMLREKLEALLPASLGQMAEVAGRWRGQVKQRLNAIGERRRFWE
CEEEEECCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTFGGRFATLVANGQTAEAQRQLEQDLEQFAQGSEGTQGEIALVGAGPGDVGLLTLRGLQ
HHCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEHHHHHHHH
VMQQADVVLYDHLVSDEILDLVRRDAERICVGKRAGAHSVIQEETNRLLVELAQQGKRVV
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEE
RLKGGDPFIFGRGGEELQVAAAAGIPFQVVPGVTAAAGATAYAGIPLTHRDHAQSVTFIT
EEECCCEEEECCCCCEEEEEEECCCCEEECCCCHHHCCCCCCCCCCCCCCCCCCEEEEEE
GHCRPDGDGLDWADLARARQTLAIYMGTMKAADISQRLIAHGRAATTPVAVISRGTRADQ
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHH
LVQTGTLQQLEQLAQQAPLPALLVIGEVVELHHQIAWFGHQPQAEGVSRPAVVNLA
HHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA