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The map label for this gene is murD [H]

Identifier: 157369004

GI number: 157369004

Start: 847065

End: 848384

Strand: Direct

Name: murD [H]

Synonym: Spro_0759

Alternate gene names: 157369004

Gene position: 847065-848384 (Clockwise)

Preceding gene: 157369003

Following gene: 157369005

Centisome position: 15.55

GC content: 59.77

Gene sequence:

>1320_bases
ATGGCGGATTATCAGGGTAAAAAAGTAGTCATCATCGGGCTGGGCCTTACCGGCCTGTCCTGTGTTGATTTCTTTATGGC
GCGCGGCGTGACGCCACGCGTTATGGATACCCGCATCTCACCGCCAGGGCTGGACAAGCTGCCTGAAAGCGTTGAGCGTC
ATCTGGGCGATTTGAATCAGGACTGGCTGTTGGCGGCGGATTTGATCGTTGCCAGCCCGGGAATGGCGCTGGCTACCCCG
GCATTAAGCGCCGCTGCGGATGCCGGCGTGGAGATCGTCGGCGACGTCGAGCTGTTCTGCCGCGAAGCGCAGGCACCGAT
CGTGGCCATCACCGGCTCTAATGGCAAAAGCACCGTCACTACCCTGGTGGGTGAGATGGCGAAAGCGGCCGGTTGGGCGG
TGGGCGTTGGCGGCAATATCGGCCTGCCTGCGCTGAGTTTGTTACGTCAGGAGTGCCAACTGTACGTGCTGGAGCTATCA
AGCTTCCAACTGGAAACCACCTTCAGCCTGCATGCGGCCGCGGCGACCATTTTGAACGTCACAGAAGATCACATGGACCG
CTACCCATTTGGCCTGCAGCAGTACCGTGCAGCCAAACTGCGCGTTTATGAAAATGCCGAGGTCTGCGTGGTCAATGCGG
ATGATGCGCTAACCATGCCGGTTCGCGGCGCCGATAAGCGCTGCATCAGTTTCGGTGCCGATGTGGGCGACTACCATTTG
AACCGTCAACTGGGCGAAACCTGGCTGCGGGTGCGTGGCGAGAAAATCCTCAATACCCGCGAGATGAAACTGACCGGCCG
TCACAACTATACCAATGCGCTGGCGGCGTTGGCATTGGCCGATGCGGTCAATATCCCGCGAGCCTCCAGTCTGAAAGCGC
TGACCACCTTTACCGGCCTGGCACATCGCTTCCAACTGGCGTGGGAACATAACGGCGTACGCTGGATTAACGATTCCAAA
GCCACCAACGTCGGCAGTACCGAAGCGGCGTTGAATGGTCTGCAGGTGGACGGCACTCTGCATCTGTTGCTGGGCGGCGA
CGGCAAGTCTGCTGACTTTTCCCCGCTGGCGCAGTATCTGCAGGGCGATAACGTCCGTCTTTACTGTTTTGGTCGTGATG
GCGAACAGCTGGCGCAGCTGCGGCCGGAGGTTGCTACGCTGACCGAGACCATGGAACAGGCGATGCAGGCGATAGCCGGT
CGGGTGCAGTCCGACGATATGGTGTTGCTGTCACCGGCATGCGCCAGCCTGGACCAGTTCCGTAATTTTGAAGTGCGCGG
CGACGAGTTCGCCCGTCTGGCACAGGAGCTCGGCGGATGA

Upstream 100 bases:

>100_bases
AATTGAAGGGCTGGCCAGAGCCGCGCGTGATCGTGCGCTTCTGGATTATTTCGCTGATGCTGGTGCTGATTGGCCTGGCG
ACGCTGAAGGTGCGGTAATT

Downstream 100 bases:

>100_bases
GACTACGCTTGCCGAACTTCGGGTTGACCGAACTGTTTAAAAACTGGGTCGCGGGATCGCGTGATAACGACTCGGTCAAT
ATGGTGTTGTACGATCGCAC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MADYQGKKVVIIGLGLTGLSCVDFFMARGVTPRVMDTRISPPGLDKLPESVERHLGDLNQDWLLAADLIVASPGMALATP
ALSAAADAGVEIVGDVELFCREAQAPIVAITGSNGKSTVTTLVGEMAKAAGWAVGVGGNIGLPALSLLRQECQLYVLELS
SFQLETTFSLHAAAATILNVTEDHMDRYPFGLQQYRAAKLRVYENAEVCVVNADDALTMPVRGADKRCISFGADVGDYHL
NRQLGETWLRVRGEKILNTREMKLTGRHNYTNALAALALADAVNIPRASSLKALTTFTGLAHRFQLAWEHNGVRWINDSK
ATNVGSTEAALNGLQVDGTLHLLLGGDGKSADFSPLAQYLQGDNVRLYCFGRDGEQLAQLRPEVATLTETMEQAMQAIAG
RVQSDDMVLLSPACASLDQFRNFEVRGDEFARLAQELGG

Sequences:

>Translated_439_residues
MADYQGKKVVIIGLGLTGLSCVDFFMARGVTPRVMDTRISPPGLDKLPESVERHLGDLNQDWLLAADLIVASPGMALATP
ALSAAADAGVEIVGDVELFCREAQAPIVAITGSNGKSTVTTLVGEMAKAAGWAVGVGGNIGLPALSLLRQECQLYVLELS
SFQLETTFSLHAAAATILNVTEDHMDRYPFGLQQYRAAKLRVYENAEVCVVNADDALTMPVRGADKRCISFGADVGDYHL
NRQLGETWLRVRGEKILNTREMKLTGRHNYTNALAALALADAVNIPRASSLKALTTFTGLAHRFQLAWEHNGVRWINDSK
ATNVGSTEAALNGLQVDGTLHLLLGGDGKSADFSPLAQYLQGDNVRLYCFGRDGEQLAQLRPEVATLTETMEQAMQAIAG
RVQSDDMVLLSPACASLDQFRNFEVRGDEFARLAQELGG
>Mature_438_residues
ADYQGKKVVIIGLGLTGLSCVDFFMARGVTPRVMDTRISPPGLDKLPESVERHLGDLNQDWLLAADLIVASPGMALATPA
LSAAADAGVEIVGDVELFCREAQAPIVAITGSNGKSTVTTLVGEMAKAAGWAVGVGGNIGLPALSLLRQECQLYVLELSS
FQLETTFSLHAAAATILNVTEDHMDRYPFGLQQYRAAKLRVYENAEVCVVNADDALTMPVRGADKRCISFGADVGDYHLN
RQLGETWLRVRGEKILNTREMKLTGRHNYTNALAALALADAVNIPRASSLKALTTFTGLAHRFQLAWEHNGVRWINDSKA
TNVGSTEAALNGLQVDGTLHLLLGGDGKSADFSPLAQYLQGDNVRLYCFGRDGEQLAQLRPEVATLTETMEQAMQAIAGR
VQSDDMVLLSPACASLDQFRNFEVRGDEFARLAQELGG

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=438, Percent_Identity=85.1598173515982, Blast_Score=757, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 47249; Mature: 47118

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADYQGKKVVIIGLGLTGLSCVDFFMARGVTPRVMDTRISPPGLDKLPESVERHLGDLNQ
CCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCC
DWLLAADLIVASPGMALATPALSAAADAGVEIVGDVELFCREAQAPIVAITGSNGKSTVT
CHHHHHHHHHCCCCCHHHCHHHHHHHCCCCEEEECHHHHHHHCCCCEEEEECCCCCHHHH
TLVGEMAKAAGWAVGVGGNIGLPALSLLRQECQLYVLELSSFQLETTFSLHAAAATILNV
HHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHEEEEEECCEEEEEEEHHHHHHHHHHHC
TEDHMDRYPFGLQQYRAAKLRVYENAEVCVVNADDALTMPVRGADKRCISFGADVGDYHL
CHHHHHCCCCCHHHHHHEEEEEECCCCEEEEECCCCEEECCCCCCCHHHHCCCCCCHHHH
NRQLGETWLRVRGEKILNTREMKLTGRHNYTNALAALALADAVNIPRASSLKALTTFTGL
HHHHHHHHHHHCCHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AHRFQLAWEHNGVRWINDSKATNVGSTEAALNGLQVDGTLHLLLGGDGKSADFSPLAQYL
HHHEEEEEECCCEEEECCCCCCCCCCHHHHHCCEEECCEEEEEECCCCCCCCHHHHHHHH
QGDNVRLYCFGRDGEQLAQLRPEVATLTETMEQAMQAIAGRVQSDDMVLLSPACASLDQF
CCCCEEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHH
RNFEVRGDEFARLAQELGG
CCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
ADYQGKKVVIIGLGLTGLSCVDFFMARGVTPRVMDTRISPPGLDKLPESVERHLGDLNQ
CCCCCCEEEEEEECCCHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCC
DWLLAADLIVASPGMALATPALSAAADAGVEIVGDVELFCREAQAPIVAITGSNGKSTVT
CHHHHHHHHHCCCCCHHHCHHHHHHHCCCCEEEECHHHHHHHCCCCEEEEECCCCCHHHH
TLVGEMAKAAGWAVGVGGNIGLPALSLLRQECQLYVLELSSFQLETTFSLHAAAATILNV
HHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHEEEEEECCEEEEEEEHHHHHHHHHHHC
TEDHMDRYPFGLQQYRAAKLRVYENAEVCVVNADDALTMPVRGADKRCISFGADVGDYHL
CHHHHHCCCCCHHHHHHEEEEEECCCCEEEEECCCCEEECCCCCCCHHHHCCCCCCHHHH
NRQLGETWLRVRGEKILNTREMKLTGRHNYTNALAALALADAVNIPRASSLKALTTFTGL
HHHHHHHHHHHCCHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AHRFQLAWEHNGVRWINDSKATNVGSTEAALNGLQVDGTLHLLLGGDGKSADFSPLAQYL
HHHEEEEEECCCEEEECCCCCCCCCCHHHHHCCEEECCEEEEEECCCCCCCCHHHHHHHH
QGDNVRLYCFGRDGEQLAQLRPEVATLTETMEQAMQAIAGRVQSDDMVLLSPACASLDQF
CCCCEEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHH
RNFEVRGDEFARLAQELGG
CCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]