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The map label for this gene is setA [H]

Identifier: 157368985

GI number: 157368985

Start: 822886

End: 824085

Strand: Direct

Name: setA [H]

Synonym: Spro_0740

Alternate gene names: 157368985

Gene position: 822886-824085 (Clockwise)

Preceding gene: 157368984

Following gene: 157368986

Centisome position: 15.1

GC content: 57.83

Gene sequence:

>1200_bases
ATGAAACGTCAGCGCTGGCTGCCACGCCGCTTTAATCCCGTCTTTGCCGCATTCCTGCTGATTGCCTTTCTTTCCGGCAT
TGCCGGCGCGCTTTTGGCGCCGACGCTGAGCCTGTTCCTGACCACCGAGGTTGAGGTGCGGCCGCTGTGGGTTGGCCTGT
TCTATACCGTCAACGCTATTGTCGGCATCGCGGTCAGTTTCCTGTTGGCCAAGCGTTCGGATACGCGCGGCGATCGTCGC
AAGTTGATTTTGCTGTGCTGCCTGATGGCGATCGGTAACTGCTTGCTGTTTGCCTTTAACCGTGACTACCTGACGCTGAT
CACCGCCGGGGTATTGCTGTCGGCGATTGCCAATACCGCGATGCCGCAGATTTTTGCGCTGGCACGAGAATATGCAGACA
GCTCGGCGCGCGAAGTGGTGATGTTCAGCTCGGTGATGCGCGCACAACTGTCGCTGGCCTGGGTGATTGGCCCGCCGCTG
TCGTTTACGCTGGCGCTGAACTACGGGTTTACCTGGATGTTCATGATTGCCGCTGCGACCTTCGCGATTTGTGCATTGCT
GGTATGGTTTACCCTGCCTTCGGTACCACGTGCGGAGCAAAGTAGTTCGGAGCTGAACGCTATTGCTTCGGTTCCGATTG
CCCCCGTCAGCGCCTGGCGCAACCGCGATGTACGTCGTTTATTTATTGCGTCGATGCTGATGTGGACCTGCAACACCATG
TATATCATCGATATGCCGCTGTATATCACCGCCGATCTGGGCCTGCCGGAAAACCTTGCCGGGCTGCTGATGGGCACGGC
CGCCGGGCTGGAGATCCCGGCCATGTTGCTGGCGGGTTACTACGTCAAACGATTCGGCAAGCGCAATATGATGCTGTTCG
CCGTGGCGGCAGGCATTCTGTTTTATGCCGGATTGGTGGTGTTTAAATTTAAGCTGGCGCTGATGGTATTACAGCTGTTC
AACGCCATCTTTATCGGCATCATCGCCGGTATTGGCATGCTCTATTTCCAGGACCTGATGCCGGGACGACCGGGGGCTGC
GACCACGCTGTTTACCAACAGTATTTCTACCGGGGTGATCCTGGCCGGGGTGCTGCAGGGGGCGCTGGTGGAAAACCTGG
GGCACTATGCGGTTTACTGGTTGGCGACTGTCCTGGCAGTAGTGGCGCTGTGGATGAGTGCCCGCGTGCGGGAAGTGTGA

Upstream 100 bases:

>100_bases
TACCCACTGGTTTTTTTATTCTCGGTCGCGGGGATATTCTTGCAATTTTGCCACCGTAGCCTAAGGTTCTGTCTTTCGCT
TTAGGGTCAGGAACGAAGAG

Downstream 100 bases:

>100_bases
GCTTAAACGTCACCAACCTTGACGCATGTCATTGAGAACATTACGCCCGCCCCCCAAGCTGAACGGACCCGGAACGCGAT
AAGTGCGTTCCCGCCCATCT

Product: sugar efflux transporter

Products: lactose [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MKRQRWLPRRFNPVFAAFLLIAFLSGIAGALLAPTLSLFLTTEVEVRPLWVGLFYTVNAIVGIAVSFLLAKRSDTRGDRR
KLILLCCLMAIGNCLLFAFNRDYLTLITAGVLLSAIANTAMPQIFALAREYADSSAREVVMFSSVMRAQLSLAWVIGPPL
SFTLALNYGFTWMFMIAAATFAICALLVWFTLPSVPRAEQSSSELNAIASVPIAPVSAWRNRDVRRLFIASMLMWTCNTM
YIIDMPLYITADLGLPENLAGLLMGTAAGLEIPAMLLAGYYVKRFGKRNMMLFAVAAGILFYAGLVVFKFKLALMVLQLF
NAIFIGIIAGIGMLYFQDLMPGRPGAATTLFTNSISTGVILAGVLQGALVENLGHYAVYWLATVLAVVALWMSARVREV

Sequences:

>Translated_399_residues
MKRQRWLPRRFNPVFAAFLLIAFLSGIAGALLAPTLSLFLTTEVEVRPLWVGLFYTVNAIVGIAVSFLLAKRSDTRGDRR
KLILLCCLMAIGNCLLFAFNRDYLTLITAGVLLSAIANTAMPQIFALAREYADSSAREVVMFSSVMRAQLSLAWVIGPPL
SFTLALNYGFTWMFMIAAATFAICALLVWFTLPSVPRAEQSSSELNAIASVPIAPVSAWRNRDVRRLFIASMLMWTCNTM
YIIDMPLYITADLGLPENLAGLLMGTAAGLEIPAMLLAGYYVKRFGKRNMMLFAVAAGILFYAGLVVFKFKLALMVLQLF
NAIFIGIIAGIGMLYFQDLMPGRPGAATTLFTNSISTGVILAGVLQGALVENLGHYAVYWLATVLAVVALWMSARVREV
>Mature_399_residues
MKRQRWLPRRFNPVFAAFLLIAFLSGIAGALLAPTLSLFLTTEVEVRPLWVGLFYTVNAIVGIAVSFLLAKRSDTRGDRR
KLILLCCLMAIGNCLLFAFNRDYLTLITAGVLLSAIANTAMPQIFALAREYADSSAREVVMFSSVMRAQLSLAWVIGPPL
SFTLALNYGFTWMFMIAAATFAICALLVWFTLPSVPRAEQSSSELNAIASVPIAPVSAWRNRDVRRLFIASMLMWTCNTM
YIIDMPLYITADLGLPENLAGLLMGTAAGLEIPAMLLAGYYVKRFGKRNMMLFAVAAGILFYAGLVVFKFKLALMVLQLF
NAIFIGIIAGIGMLYFQDLMPGRPGAATTLFTNSISTGVILAGVLQGALVENLGHYAVYWLATVLAVVALWMSARVREV

Specific function: Involved in the efflux of sugars. The physiological role may be the detoxification of non-metabolizable sugar analogs. Can transport IPTG, lactose and glucose. Has broad substrate specificity, with preferences for glucosides or galactosides with alkyl or

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Set transporter family [H]

Homologues:

Organism=Escherichia coli, GI48994875, Length=393, Percent_Identity=69.9745547073791, Blast_Score=560, Evalue=1e-161,
Organism=Escherichia coli, GI1790091, Length=367, Percent_Identity=52.5885558583106, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI1788495, Length=392, Percent_Identity=52.2959183673469, Blast_Score=378, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004750 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43750; Mature: 43750

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRQRWLPRRFNPVFAAFLLIAFLSGIAGALLAPTLSLFLTTEVEVRPLWVGLFYTVNAI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEEEHHHHHHHHHHHHH
VGIAVSFLLAKRSDTRGDRRKLILLCCLMAIGNCLLFAFNRDYLTLITAGVLLSAIANTA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEHCCCCHHHHHHHHHHHHHHHHHH
MPQIFALAREYADSSAREVVMFSSVMRAQLSLAWVIGPPLSFTLALNYGFTWMFMIAAAT
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCHHHHHHHHHHH
FAICALLVWFTLPSVPRAEQSSSELNAIASVPIAPVSAWRNRDVRRLFIASMLMWTCNTM
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHCCEE
YIIDMPLYITADLGLPENLAGLLMGTAAGLEIPAMLLAGYYVKRFGKRNMMLFAVAAGIL
EEEECCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
FYAGLVVFKFKLALMVLQLFNAIFIGIIAGIGMLYFQDLMPGRPGAATTLFTNSISTGVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHH
LAGVLQGALVENLGHYAVYWLATVLAVVALWMSARVREV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRQRWLPRRFNPVFAAFLLIAFLSGIAGALLAPTLSLFLTTEVEVRPLWVGLFYTVNAI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEEEHHHHHHHHHHHHH
VGIAVSFLLAKRSDTRGDRRKLILLCCLMAIGNCLLFAFNRDYLTLITAGVLLSAIANTA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEHCCCCHHHHHHHHHHHHHHHHHH
MPQIFALAREYADSSAREVVMFSSVMRAQLSLAWVIGPPLSFTLALNYGFTWMFMIAAAT
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCHHHHHHHHHHH
FAICALLVWFTLPSVPRAEQSSSELNAIASVPIAPVSAWRNRDVRRLFIASMLMWTCNTM
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHCCEE
YIIDMPLYITADLGLPENLAGLLMGTAAGLEIPAMLLAGYYVKRFGKRNMMLFAVAAGIL
EEEECCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
FYAGLVVFKFKLALMVLQLFNAIFIGIIAGIGMLYFQDLMPGRPGAATTLFTNSISTGVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHH
LAGVLQGALVENLGHYAVYWLATVLAVVALWMSARVREV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactose [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: lactose [Cytoplasm] + Proton [Periplasm] = lactose [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 10209755; 10438463 [H]