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The map label for this gene is proP [H]

Identifier: 157368935

GI number: 157368935

Start: 760211

End: 761518

Strand: Direct

Name: proP [H]

Synonym: Spro_0690

Alternate gene names: 157368935

Gene position: 760211-761518 (Clockwise)

Preceding gene: 157368934

Following gene: 157368937

Centisome position: 13.95

GC content: 57.8

Gene sequence:

>1308_bases
ATGCCTCACGATAATAATCGCCGGTTAAACCGGCAAGACTACAAAACTCTGACGTTAGCTGCCCTGGGCGGTGCGCTGGA
GTTCTATGATTTCATCATTTTTGTCTTCTTTGCGGCGGTGATGGGCGAGCTGTTTTTCCCGGCGGATATGCCGGAATGGC
TACGTCAGGTACAAACCTTCGGCATTTTTGCCGCCGGTTATCTGGCCCGCCCGCTGGGCGGCATTGTGATGGCGCACTTC
GGCGATCTGGTCGGCCGCAAAAAAATGTTCAGCCTGAGTATTTTGCTGATGGCGTTGCCGACATTGGCGATGGGTCTGTT
ACCGACCTATGCCAGCATCGGCATTGCCGCACCGCTGTTGCTGCTGCTGATGCGTGTGCTGCAAGGGGCGGCGATCGGCG
GCGAGGTGCCGGGGGCCTGGGTGTTTGTGGCTGAACACGTGCCGCGTCGGCGCATCGGTTTTGCCTGCGGCACGCTGACT
GCCGGCCTGACCGTCGGCATCCTGCTGGGCTCGCTGGTGGCGACGGTAATCAACACCACGCTTAGTCAACAGACCATTAT
TGACGGTGGCTGGCGTATCCCGTTCCTGCTGGGTGGCATTTTCGGCCTGGTGGCGATGTACCTGCGCCGCTGGCTGCAGG
AAACACCGATCTTCATCGAGATGCAGGCGCGTAAGGCCTTGGCAGAAGAGCTGCCGTTGAAATCGGTGGTGCTCAATCAT
CGCAAGGAAGTGGTGGTGTCGATGCTGCTGACCTGGCTATTGTCCGCCGGTATTGTGGTGGTGATCCTGATGACCCCGAC
CTACCTGCAAAAACAGTTTGCTATCGCCCCGGCGTTATCGCTGCAGGCCAATAGCATCGCCACCATCATGCTGATCGCCG
GTTGCATCATCGCCGGCTTGTCGGCGGATCGCTTTGGCGCCAGCAAAACCTTTATCGTCGGCAGCCTGCTGCTGGCCGGT
TGCAGTTGGCTATTTTATCGGACGGTGGGCGAACACCCGGAAATGTTGTTTGCCTGCTACGCGCTGGTGGGTTTCAGCGT
AGGCGTAGTGGGCGCGGTCCCCTACGTGATGGTGCGGGCTTTTCCGGCGGAAGTGCGATTTACCGGGATTTCGTTCTCAT
ATAATGTCGCATACGCCATTTTTGGCGGTTTAACCCCAATATTTGTGACGTTGATCATGAAGTTAACCCCGTTAGCGCCC
GCTTACTATGTGCTGGCGCTGTCGGGCATCGGATTGCTGCTGGGGATTTATCTGCATCGCGATCTGAACAGCGAAACCAA
GGCACAGCGCGCCGGTTCCTACAGCTGA

Upstream 100 bases:

>100_bases
GCTAGCCGATCTGGTGTGAAAATAATTTGCCGTTATAGTAAGGATTCGTTTACAGAAAGCCGCTGGCTTTTATTTTCACA
GTTTCCTCCGACGGTACCCT

Downstream 100 bases:

>100_bases
AAAAAAAGCCCTGCAATCAACCGCAGGGCTTCTTGTTTCAGGCCAGTGGCGTTTATGCCACTGCCCGAATTGGTTCTACA
TTGTCGACTGTTGGCTCGCC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MPHDNNRRLNRQDYKTLTLAALGGALEFYDFIIFVFFAAVMGELFFPADMPEWLRQVQTFGIFAAGYLARPLGGIVMAHF
GDLVGRKKMFSLSILLMALPTLAMGLLPTYASIGIAAPLLLLLMRVLQGAAIGGEVPGAWVFVAEHVPRRRIGFACGTLT
AGLTVGILLGSLVATVINTTLSQQTIIDGGWRIPFLLGGIFGLVAMYLRRWLQETPIFIEMQARKALAEELPLKSVVLNH
RKEVVVSMLLTWLLSAGIVVVILMTPTYLQKQFAIAPALSLQANSIATIMLIAGCIIAGLSADRFGASKTFIVGSLLLAG
CSWLFYRTVGEHPEMLFACYALVGFSVGVVGAVPYVMVRAFPAEVRFTGISFSYNVAYAIFGGLTPIFVTLIMKLTPLAP
AYYVLALSGIGLLLGIYLHRDLNSETKAQRAGSYS

Sequences:

>Translated_435_residues
MPHDNNRRLNRQDYKTLTLAALGGALEFYDFIIFVFFAAVMGELFFPADMPEWLRQVQTFGIFAAGYLARPLGGIVMAHF
GDLVGRKKMFSLSILLMALPTLAMGLLPTYASIGIAAPLLLLLMRVLQGAAIGGEVPGAWVFVAEHVPRRRIGFACGTLT
AGLTVGILLGSLVATVINTTLSQQTIIDGGWRIPFLLGGIFGLVAMYLRRWLQETPIFIEMQARKALAEELPLKSVVLNH
RKEVVVSMLLTWLLSAGIVVVILMTPTYLQKQFAIAPALSLQANSIATIMLIAGCIIAGLSADRFGASKTFIVGSLLLAG
CSWLFYRTVGEHPEMLFACYALVGFSVGVVGAVPYVMVRAFPAEVRFTGISFSYNVAYAIFGGLTPIFVTLIMKLTPLAP
AYYVLALSGIGLLLGIYLHRDLNSETKAQRAGSYS
>Mature_434_residues
PHDNNRRLNRQDYKTLTLAALGGALEFYDFIIFVFFAAVMGELFFPADMPEWLRQVQTFGIFAAGYLARPLGGIVMAHFG
DLVGRKKMFSLSILLMALPTLAMGLLPTYASIGIAAPLLLLLMRVLQGAAIGGEVPGAWVFVAEHVPRRRIGFACGTLTA
GLTVGILLGSLVATVINTTLSQQTIIDGGWRIPFLLGGIFGLVAMYLRRWLQETPIFIEMQARKALAEELPLKSVVLNHR
KEVVVSMLLTWLLSAGIVVVILMTPTYLQKQFAIAPALSLQANSIATIMLIAGCIIAGLSADRFGASKTFIVGSLLLAGC
SWLFYRTVGEHPEMLFACYALVGFSVGVVGAVPYVMVRAFPAEVRFTGISFSYNVAYAIFGGLTPIFVTLIMKLTPLAPA
YYVLALSGIGLLLGIYLHRDLNSETKAQRAGSYS

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=429, Percent_Identity=28.6713286713287, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1788292, Length=409, Percent_Identity=29.8288508557457, Blast_Score=161, Evalue=9e-41,
Organism=Escherichia coli, GI1789941, Length=444, Percent_Identity=31.7567567567568, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1788942, Length=438, Percent_Identity=31.0502283105023, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI87082231, Length=502, Percent_Identity=23.9043824701195, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87082404, Length=422, Percent_Identity=24.1706161137441, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47155; Mature: 47024

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCHHHHHHCCCCC
>Mature Secondary Structure 
PHDNNRRLNRQDYKTLTLAALGGALEFYDFIIFVFFAAVMGELFFPADMPEWLRQVQTF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH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CCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA