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The map label for this gene is radA [H]

Identifier: 157368912

GI number: 157368912

Start: 733171

End: 734556

Strand: Direct

Name: radA [H]

Synonym: Spro_0667

Alternate gene names: 157368912

Gene position: 733171-734556 (Clockwise)

Preceding gene: 157368911

Following gene: 157368913

Centisome position: 13.46

GC content: 59.67

Gene sequence:

>1386_bases
GTGGCAAAAGCAGCAAAACGGGCGTTTGTATGTAATGAGTGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGCGC
TTGCCACGCCTGGAACAGCATTACAGAAGTGCGTCTGGCTGCGGTGTCTTCCTCTTCTCGCAATGAGCGCCTGAGCGGCT
ACGCCGGCGATGCCGGCATCAGCAAGGTGCAAAAACTGTCGGACATCAGCCTGGATGAGCTGCCGCGTTTCTCCACCGGT
TTCCTTGAGTTTGACCGGGTGCTGGGGGGCGGGGTGGTGCCGGGCAGTGCGATCCTGATCGGCGGTAACCCTGGCGCCGG
CAAGAGTACGCTGCTGTTGCAGGTGCTGTGCAAACTTTCCGAACAGATGAAAACCCTGTACGTCACCGGTGAAGAATCAT
TGCAGCAGGTCGCGATGCGCGCCCACCGTCTCGGCTTGCCGACTGGCGGCCTGAATATGCTGTCGGAAACCAGTATCGAG
CAGATCTGCCTGATTGCCGAGCAGGAACAGCCGAAACTGATGGTGATTGACTCCATCCAGGTGATGCACATGGCGGACAT
TCAGTCTTCGCCGGGCAGCGTAGCGCAGGTGCGTGAAACCGCCGCCTACCTGACGCGCTTCGCCAAGACGCGCGGTGTGG
CGATTGTGATGGTGGGACACGTCACCAAAGACGGCTCTCTGGCCGGGCCGAAAGTTTTGGAACACTGTATTGACTGCTCG
GTGCTGCTGGACGGCGACGCCGATTCGCGTTTTCGTACCCTGCGCAGCCACAAAAACCGCTTTGGTGCGGTGAATGAATT
GGGCGTTTTCGCCATGACAGAGCAGGGCCTGCGTGAAGTCAGCAACCCATCGGCCATTTTCCTCAGCCGTGGCGATGAGG
TCACTTCCGGCAGTTCGGTGATGGTGGTGTGGGAAGGCACGCGGCCGTTGCTGGTGGAAATTCAGGCGCTGGTGGATCAT
TCGATGATGTCCAATCCGCGTCGGGTGGCCGTGGGTCTGGAACAAAACCGATTGGCGATCCTGCTGGCGGTGCTGCATCG
CCACGGCGGGCTGCAGATGTCGGATCAGGACGTGTTTGTTAACGTGGTTGGCGGGGTGAAAGTCAGTGAAACCAGCGCCG
ATCTGGCGTTGCTGATGTCGCTGGTGTCCAGCCTGCGCGATCGCCCGCTGCCAAACGATTTGGTGGTGTTTGGCGAAGTA
GGGCTGGCAGGGGAGATCCGTCCGGTGCCGAGCGGCCAGGAACGTATTTCCGAAGCGGCGAAACACGGTTTCAAGCGGGC
CATCGTGCCACATGCCAACGTACCCAAGAAACCACCGGCCAATATGCAGGTGTTCGGCGTGAAGAAACTGGCGGACGCAT
TGGACGTGCTGGAACAGTTTTATTAA

Upstream 100 bases:

>100_bases
GTGTACTGACCGGTAGCCTGAAACACGAAGTTCGCTAAAGTTGACCCTGATCCGGCGATAATGCCAGACAGCGGCAATGT
GGTAAAAACAGAGGTAATAC

Downstream 100 bases:

>100_bases
TTGCCACGCGGTGTGCAGATTATGCTATTTTGTTTAGCATATTAAACGGAGGGGCTATGCGGCAATTCGATTACCTGAAG
GCGTCGATTAAGCAGAAGAG

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MAKAAKRAFVCNECGADYPRWQGQCSACHAWNSITEVRLAAVSSSSRNERLSGYAGDAGISKVQKLSDISLDELPRFSTG
FLEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQVLCKLSEQMKTLYVTGEESLQQVAMRAHRLGLPTGGLNMLSETSIE
QICLIAEQEQPKLMVIDSIQVMHMADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMSNPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFVNVVGGVKVSETSADLALLMSLVSSLRDRPLPNDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFKRAIVPHANVPKKPPANMQVFGVKKLADALDVLEQFY

Sequences:

>Translated_461_residues
MAKAAKRAFVCNECGADYPRWQGQCSACHAWNSITEVRLAAVSSSSRNERLSGYAGDAGISKVQKLSDISLDELPRFSTG
FLEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQVLCKLSEQMKTLYVTGEESLQQVAMRAHRLGLPTGGLNMLSETSIE
QICLIAEQEQPKLMVIDSIQVMHMADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMSNPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFVNVVGGVKVSETSADLALLMSLVSSLRDRPLPNDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFKRAIVPHANVPKKPPANMQVFGVKKLADALDVLEQFY
>Mature_460_residues
AKAAKRAFVCNECGADYPRWQGQCSACHAWNSITEVRLAAVSSSSRNERLSGYAGDAGISKVQKLSDISLDELPRFSTGF
LEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQVLCKLSEQMKTLYVTGEESLQQVAMRAHRLGLPTGGLNMLSETSIEQ
ICLIAEQEQPKLMVIDSIQVMHMADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCSV
LLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHS
MMSNPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFVNVVGGVKVSETSADLALLMSLVSSLRDRPLPNDLVVFGEVG
LAGEIRPVPSGQERISEAAKHGFKRAIVPHANVPKKPPANMQVFGVKKLADALDVLEQFY

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=460, Percent_Identity=91.7391304347826, Blast_Score=874, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49608; Mature: 49477

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAAKRAFVCNECGADYPRWQGQCSACHAWNSITEVRLAAVSSSSRNERLSGYAGDAGI
CCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
SKVQKLSDISLDELPRFSTGFLEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQVLCKLS
HHHHHHHCCCHHHCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHH
EQMKTLYVTGEESLQQVAMRAHRLGLPTGGLNMLSETSIEQICLIAEQEQPKLMVIDSIQ
HHHHEEEEECHHHHHHHHHHHHHCCCCCCCCHHHHHHCHHHEEEEECCCCCCEEEEECEE
VMHMADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
EEEEHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHHHHHHCCCE
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSV
EEEECCCCHHHHHHHHHHHHCCCHHHCCHHEECHHHHHHCCCCCEEEEECCCCCCCCCEE
MVVWEGTRPLLVEIQALVDHSMMSNPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFV
EEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEE
NVVGGVKVSETSADLALLMSLVSSLRDRPLPNDLVVFGEVGLAGEIRPVPSGQERISEAA
EEECCEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHH
KHGFKRAIVPHANVPKKPPANMQVFGVKKLADALDVLEQFY
HCCHHEECCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKAAKRAFVCNECGADYPRWQGQCSACHAWNSITEVRLAAVSSSSRNERLSGYAGDAGI
CCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
SKVQKLSDISLDELPRFSTGFLEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQVLCKLS
HHHHHHHCCCHHHCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHH
EQMKTLYVTGEESLQQVAMRAHRLGLPTGGLNMLSETSIEQICLIAEQEQPKLMVIDSIQ
HHHHEEEEECHHHHHHHHHHHHHCCCCCCCCHHHHHHCHHHEEEEECCCCCCEEEEECEE
VMHMADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
EEEEHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHHHHHHCCCE
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSV
EEEECCCCHHHHHHHHHHHHCCCHHHCCHHEECHHHHHHCCCCCEEEEECCCCCCCCCEE
MVVWEGTRPLLVEIQALVDHSMMSNPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFV
EEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEE
NVVGGVKVSETSADLALLMSLVSSLRDRPLPNDLVVFGEVGLAGEIRPVPSGQERISEAA
EEECCEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHH
KHGFKRAIVPHANVPKKPPANMQVFGVKKLADALDVLEQFY
HCCHHEECCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1327967; 8759876; 7610040; 9278503 [H]