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The map label for this gene is hpcB [H]

Identifier: 157368873

GI number: 157368873

Start: 693926

End: 694783

Strand: Direct

Name: hpcB [H]

Synonym: Spro_0628

Alternate gene names: 157368873

Gene position: 693926-694783 (Clockwise)

Preceding gene: 157368872

Following gene: 157368874

Centisome position: 12.74

GC content: 56.06

Gene sequence:

>858_bases
ATGGGCAAATTGGCTTTAGCAGCAAAGATCACTCATGTGCCGTCAATGTATCTGTCGGAAATGCCTGGAAAAAACCATGG
TTGCCGCCAGGCGGCGATCGACGGTCATAAAGAGATCAGCGCGCGCTGCCGTGAAATGGGCGTCGACACCATTATTGTGT
TCGACACCCACTGGCTGGTGAACAGCGCCTATCACATCAACTGCAGCCCGCATTTCGAAGGGCTGTATACCAGCAACGAA
CTGCCGCACTTCATCCGCGACATGACCTACGAATACGAAGGCAACCCGGCACTGGGTCAGTTGATTGCCGCTGAAGCCTG
CAAACTCGGGGTACGCGCCAAAGCGCATGAAATCCCAAGCCTGAAGCTGGAGTACGGCACGCTGGTGCCGATGCGCTACA
TGAATAGCGACAAACATTTCAAAGTGATCTCGATTTCGGCGTTTTGCACCGTGCATGACTTTGCCGACAGTCGTCGGTTA
GGTGAGGCGATCAAAACCGCGGTTGAGAAATACGACGGGACGGTGGCGGTATTGGCCAGCGGTTCGCTTTCACACCGTTT
TATCGACGATCAGCGTGCCGAAGAAGGTATGAACAGCTACACCCGCCAGTTTGACCAGCAGATGGATGAACGGGTGGTCA
AGCTGTGGGAAGAAGGGCGCTTTCGCGAGTTCTGCGAAATGCTGCCGGAGTACGCCGACTACTGCTACGGCGAGGGCAAC
ATGCATGACACCGTGATGCTGCTCGGCCTGCTGGGCTGGGACAAATATGACGGCAAGGTGGAGTTTGTTACCCCGCTGTT
TGCCAGTTCCGGCACCGGGCAAGTCAACGCCATCTTCCCGCTGCCGGCCGAAGCCTGA

Upstream 100 bases:

>100_bases
AGGCGGGGAATACAGTTTCGAAGTATTCGCCGAGATGAAGAACGTGTGTATTTCCATGGGGGATCACCCGATCCCACGTT
GGGGCGTCTAGGAGGGTTAT

Downstream 100 bases:

>100_bases
GGAGTCTCCATGCCCCATTTTTACGCTGAATGTACCGACAACATTCGCCACGAGGCGGATTTGCCCGCGCTGTTTGCCAG
GGTCAATCAGGCACTGGCGG

Product: 3,4-dihydroxyphenylacetate 2,3-dioxygenase

Products: NA

Alternate protein names: Homoprotocatechuate 2,3-dioxygenase; HPC dioxygenase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MGKLALAAKITHVPSMYLSEMPGKNHGCRQAAIDGHKEISARCREMGVDTIIVFDTHWLVNSAYHINCSPHFEGLYTSNE
LPHFIRDMTYEYEGNPALGQLIAAEACKLGVRAKAHEIPSLKLEYGTLVPMRYMNSDKHFKVISISAFCTVHDFADSRRL
GEAIKTAVEKYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTRQFDQQMDERVVKLWEEGRFREFCEMLPEYADYCYGEGN
MHDTVMLLGLLGWDKYDGKVEFVTPLFASSGTGQVNAIFPLPAEA

Sequences:

>Translated_285_residues
MGKLALAAKITHVPSMYLSEMPGKNHGCRQAAIDGHKEISARCREMGVDTIIVFDTHWLVNSAYHINCSPHFEGLYTSNE
LPHFIRDMTYEYEGNPALGQLIAAEACKLGVRAKAHEIPSLKLEYGTLVPMRYMNSDKHFKVISISAFCTVHDFADSRRL
GEAIKTAVEKYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTRQFDQQMDERVVKLWEEGRFREFCEMLPEYADYCYGEGN
MHDTVMLLGLLGWDKYDGKVEFVTPLFASSGTGQVNAIFPLPAEA
>Mature_284_residues
GKLALAAKITHVPSMYLSEMPGKNHGCRQAAIDGHKEISARCREMGVDTIIVFDTHWLVNSAYHINCSPHFEGLYTSNEL
PHFIRDMTYEYEGNPALGQLIAAEACKLGVRAKAHEIPSLKLEYGTLVPMRYMNSDKHFKVISISAFCTVHDFADSRRLG
EAIKTAVEKYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTRQFDQQMDERVVKLWEEGRFREFCEMLPEYADYCYGEGNM
HDTVMLLGLLGWDKYDGKVEFVTPLFASSGTGQVNAIFPLPAEA

Specific function: Transforms homoprotocatechuic acid (HPC) into 5- carboxymethyl-2-hydroxy-muconic semialdehyde (CHMS) [H]

COG id: COG3384

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011984
- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: =1.13.11.15 [H]

Molecular weight: Translated: 32037; Mature: 31906

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLALAAKITHVPSMYLSEMPGKNHGCRQAAIDGHKEISARCREMGVDTIIVFDTHWLV
CCCHHHHHHHHHCCHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHCCCCEEEEEECHHHH
NSAYHINCSPHFEGLYTSNELPHFIRDMTYEYEGNPALGQLIAAEACKLGVRAKAHEIPS
CCEEEEECCCCCCCEECCCCCHHHHHHCCEEECCCCHHHHHHHHHHHHHCCCHHHHCCCC
LKLEYGTLVPMRYMNSDKHFKVISISAFCTVHDFADSRRLGEAIKTAVEKYDGTVAVLAS
EEEECCCCCCHHHCCCCCCEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
GSLSHRFIDDQRAEEGMNSYTRQFDQQMDERVVKLWEEGRFREFCEMLPEYADYCYGEGN
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
MHDTVMLLGLLGWDKYDGKVEFVTPLFASSGTGQVNAIFPLPAEA
CHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCEEEEECCCCCC
>Mature Secondary Structure 
GKLALAAKITHVPSMYLSEMPGKNHGCRQAAIDGHKEISARCREMGVDTIIVFDTHWLV
CCHHHHHHHHHCCHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHCCCCEEEEEECHHHH
NSAYHINCSPHFEGLYTSNELPHFIRDMTYEYEGNPALGQLIAAEACKLGVRAKAHEIPS
CCEEEEECCCCCCCEECCCCCHHHHHHCCEEECCCCHHHHHHHHHHHHHCCCHHHHCCCC
LKLEYGTLVPMRYMNSDKHFKVISISAFCTVHDFADSRRLGEAIKTAVEKYDGTVAVLAS
EEEECCCCCCHHHCCCCCCEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
GSLSHRFIDDQRAEEGMNSYTRQFDQQMDERVVKLWEEGRFREFCEMLPEYADYCYGEGN
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
MHDTVMLLGLLGWDKYDGKVEFVTPLFASSGTGQVNAIFPLPAEA
CHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2261999 [H]