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The map label for this gene is hpaG [H]

Identifier: 157368871

GI number: 157368871

Start: 691701

End: 692465

Strand: Direct

Name: hpaG [H]

Synonym: Spro_0626

Alternate gene names: 157368871

Gene position: 691701-692465 (Clockwise)

Preceding gene: 157368870

Following gene: 157368872

Centisome position: 12.69

GC content: 58.56

Gene sequence:

>765_bases
ATGAAACACGCCCGTATTCGCCATCACGGCCAAATCTTTAACGTCAGCGTTGACCAGCAACTGCGTGTCACCCTGCCGGA
CGGCAGCGTGCTCAATGAACTGGACGTCGAATGGCTGCCACCGGCGCAGGGCACGGTATTTGCCCTGGGCCTGAACTATG
CCGACCATGCCAGCGAACTGGAATTCAAGGCACCGGAAGAACCGCTGGTGTTCCTGAAAGCCCCCAACACCCTGACCGGC
CACCGTCAGGTGTCGGTGCGCCCGGCCGACGTCGAGTATATGCACTACGAAGCCGAGCTGGTGGCGGTGATCGGCAAAAC
GGCACGCAACGTCAGCCGTGAGAACGCCATGGACTATGTAGCGGGTTACACCCTGTGTAACGACTACGCCATCCGCGACT
ATCTGGAAAATTATTACCGTCCGAACCTGCGGGTGAAAAGCCGCGACACCCTGACGCCGATTGGGCCGTACATCGTCGAT
CGCGATGATATCGCCGATCCGCACCGTCTGGCGCTCAGCACTTACGTCAACGGTGAGCTGCGCCAGCGCGGCAGCACCGG
CGACATGATTTTTGATATCCCCTATTTGATCAGTTACCTGAGCGAATTTATGACGCTGCAGCCGGGCGATATGATCGCCA
CCGGCACGCCGAAAGGGTTGGCCGACGTGCAACCGGGGGACGAAGTGGTGGTGGAGATCGAGGGCATTGGCCGTCTGGTT
AACCACATCATCAGTGAAAAAGATTACGAGGAAAGCCTGCGATGA

Upstream 100 bases:

>100_bases
CGCACCAGCGGGTGGAGATTAAACCGGGGGATGAAGTGACGGTACGCGCCGCCGGTCTGCCGACCCTGACCAACCGCGTG
ACGCAGGCAGGAGGAGCATC

Downstream 100 bases:

>100_bases
AAACCATCAACCATTGGATTAACGGTAAAAACGTAGCCAGCAAAGAGTACTTCACCACCACCAACCCGGCCAACGGTGAG
GTGCTGGCGGAAGTGGCGTC

Product: 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase subunit HpaG2

Products: NA

Alternate protein names: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase; HHDD isomerase; 5-carboxymethyl-2-hydroxymuconate Delta-isomerase; 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase; OPET decarboxylase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKHARIRHHGQIFNVSVDQQLRVTLPDGSVLNELDVEWLPPAQGTVFALGLNYADHASELEFKAPEEPLVFLKAPNTLTG
HRQVSVRPADVEYMHYEAELVAVIGKTARNVSRENAMDYVAGYTLCNDYAIRDYLENYYRPNLRVKSRDTLTPIGPYIVD
RDDIADPHRLALSTYVNGELRQRGSTGDMIFDIPYLISYLSEFMTLQPGDMIATGTPKGLADVQPGDEVVVEIEGIGRLV
NHIISEKDYEESLR

Sequences:

>Translated_254_residues
MKHARIRHHGQIFNVSVDQQLRVTLPDGSVLNELDVEWLPPAQGTVFALGLNYADHASELEFKAPEEPLVFLKAPNTLTG
HRQVSVRPADVEYMHYEAELVAVIGKTARNVSRENAMDYVAGYTLCNDYAIRDYLENYYRPNLRVKSRDTLTPIGPYIVD
RDDIADPHRLALSTYVNGELRQRGSTGDMIFDIPYLISYLSEFMTLQPGDMIATGTPKGLADVQPGDEVVVEIEGIGRLV
NHIISEKDYEESLR
>Mature_254_residues
MKHARIRHHGQIFNVSVDQQLRVTLPDGSVLNELDVEWLPPAQGTVFALGLNYADHASELEFKAPEEPLVFLKAPNTLTG
HRQVSVRPADVEYMHYEAELVAVIGKTARNVSRENAMDYVAGYTLCNDYAIRDYLENYYRPNLRVKSRDTLTPIGPYIVD
RDDIADPHRLALSTYVNGELRQRGSTGDMIFDIPYLISYLSEFMTLQPGDMIATGTPKGLADVQPGDEVVVEIEGIGRLV
NHIISEKDYEESLR

Specific function: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5- tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7- dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate) [H]

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=225, Percent_Identity=36.4444444444444, Blast_Score=157, Evalue=9e-39,
Organism=Homo sapiens, GI40786394, Length=207, Percent_Identity=37.1980676328502, Blast_Score=155, Evalue=2e-38,
Organism=Homo sapiens, GI215422413, Length=197, Percent_Identity=41.1167512690355, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI66348062, Length=194, Percent_Identity=41.7525773195876, Blast_Score=150, Evalue=8e-37,
Organism=Homo sapiens, GI13654274, Length=194, Percent_Identity=41.7525773195876, Blast_Score=150, Evalue=9e-37,
Organism=Escherichia coli, GI1787428, Length=193, Percent_Identity=38.3419689119171, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17557057, Length=187, Percent_Identity=37.9679144385027, Blast_Score=136, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6324161, Length=229, Percent_Identity=29.2576419213974, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28572127, Length=221, Percent_Identity=42.5339366515837, Blast_Score=167, Evalue=9e-42,
Organism=Drosophila melanogaster, GI24663695, Length=222, Percent_Identity=33.3333333333333, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI28571789, Length=204, Percent_Identity=37.7450980392157, Blast_Score=132, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012684
- InterPro:   IPR012686 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =5.3.3.10; =4.1.1.68 [H]

Molecular weight: Translated: 28605; Mature: 28605

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHARIRHHGQIFNVSVDQQLRVTLPDGSVLNELDVEWLPPAQGTVFALGLNYADHASEL
CCCCCEECCCEEEEEECCCEEEEECCCCCCHHHCCCEEECCCCCEEEEEECCCCCCCCCC
EFKAPEEPLVFLKAPNTLTGHRQVSVRPADVEYMHYEAELVAVIGKTARNVSRENAMDYV
CCCCCCCCEEEEECCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
AGYTLCNDYAIRDYLENYYRPNLRVKSRDTLTPIGPYIVDRDDIADPHRLALSTYVNGEL
HHHHHHCHHHHHHHHHHHCCCCCEECCCCCCCCCCCEEECCCCCCCHHHEEEEHHCCCHH
RQRGSTGDMIFDIPYLISYLSEFMTLQPGDMIATGTPKGLADVQPGDEVVVEIEGIGRLV
HCCCCCCCEEEEHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCEEEEEEHHHHHHH
NHIISEKDYEESLR
HHHHCCCCHHHHCC
>Mature Secondary Structure
MKHARIRHHGQIFNVSVDQQLRVTLPDGSVLNELDVEWLPPAQGTVFALGLNYADHASEL
CCCCCEECCCEEEEEECCCEEEEECCCCCCHHHCCCEEECCCCCEEEEEECCCCCCCCCC
EFKAPEEPLVFLKAPNTLTGHRQVSVRPADVEYMHYEAELVAVIGKTARNVSRENAMDYV
CCCCCCCCEEEEECCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
AGYTLCNDYAIRDYLENYYRPNLRVKSRDTLTPIGPYIVDRDDIADPHRLALSTYVNGEL
HHHHHHCHHHHHHHHHHHCCCCCEECCCCCCCCCCCEEECCCCCCCHHHEEEEHHCCCHH
RQRGSTGDMIFDIPYLISYLSEFMTLQPGDMIATGTPKGLADVQPGDEVVVEIEGIGRLV
HCCCCCCCEEEEHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCEEEEEEHHHHHHH
NHIISEKDYEESLR
HHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA