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The map label for this gene is hpaG [H]

Identifier: 157368870

GI number: 157368870

Start: 691072

End: 691704

Strand: Direct

Name: hpaG [H]

Synonym: Spro_0625

Alternate gene names: 157368870

Gene position: 691072-691704 (Clockwise)

Preceding gene: 157368868

Following gene: 157368871

Centisome position: 12.68

GC content: 60.66

Gene sequence:

>633_bases
ATGAAAGGCACCGTTTTTTCCGTCGCGTTGAACCACCGCAGCCAGATGGACGCCTGGGATCAGGCGTTTCATCAGCCACC
TTATAAAACCCCGCCGAAGACCCCGGTGTGGTTTATCAAACCCCGTAATACCCACCTCGCCAACGGCGGGGCGATCCCGT
TTCCTGCCGGTGAGCAAGTGCAGAGCGGCGCCACGCTGGCGGTGGTGATTGGCAACACCGCGCGCAAGGTTCCTGCCGAA
CGGGTGGCCGATTATCTGGCCGGCTATGCGTTGGCCAATGATGTCAGCCTGCCGGAAAGCAGTTTTTACCGCCCGGCGAT
CAAAGCGAAATGCCGCGATGGCTTTTGTCCCTTGGGGGATATCGGTCAATTGGCAACCAGCGACAGGCTGGAAATTATCA
CCGAAATCAACGGTGTGGAGCAGGACCGCTGGTCGACGGCCGATCTGGTACGTTCGGTGCCGGAACTGATCGCCGCCATC
AGCGATTTTATTACCCTGCAGCCGGGCGACGCGGTGCTGATCGGAACGCCGCACCAGCGGGTGGAGATTAAACCGGGGGA
TGAAGTGACGGTACGCGCCGCCGGTCTGCCGACCCTGACCAACCGCGTGACGCAGGCAGGAGGAGCATCATGA

Upstream 100 bases:

>100_bases
AATCCTCCCGCGTTGCGTAAAAGCTGAACAGCGTATAAAAGTAATCATTAACATATTAATGAATGCCGGTTGTCATGTTC
TGTCATTGAAAGGAGTTTGC

Downstream 100 bases:

>100_bases
AACACGCCCGTATTCGCCATCACGGCCAAATCTTTAACGTCAGCGTTGACCAGCAACTGCGTGTCACCCTGCCGGACGGC
AGCGTGCTCAATGAACTGGA

Product: 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase subunit HpaG1

Products: NA

Alternate protein names: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase; HHDD isomerase; 5-carboxymethyl-2-hydroxymuconate Delta-isomerase; 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase; OPET decarboxylase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MKGTVFSVALNHRSQMDAWDQAFHQPPYKTPPKTPVWFIKPRNTHLANGGAIPFPAGEQVQSGATLAVVIGNTARKVPAE
RVADYLAGYALANDVSLPESSFYRPAIKAKCRDGFCPLGDIGQLATSDRLEIITEINGVEQDRWSTADLVRSVPELIAAI
SDFITLQPGDAVLIGTPHQRVEIKPGDEVTVRAAGLPTLTNRVTQAGGAS

Sequences:

>Translated_210_residues
MKGTVFSVALNHRSQMDAWDQAFHQPPYKTPPKTPVWFIKPRNTHLANGGAIPFPAGEQVQSGATLAVVIGNTARKVPAE
RVADYLAGYALANDVSLPESSFYRPAIKAKCRDGFCPLGDIGQLATSDRLEIITEINGVEQDRWSTADLVRSVPELIAAI
SDFITLQPGDAVLIGTPHQRVEIKPGDEVTVRAAGLPTLTNRVTQAGGAS
>Mature_210_residues
MKGTVFSVALNHRSQMDAWDQAFHQPPYKTPPKTPVWFIKPRNTHLANGGAIPFPAGEQVQSGATLAVVIGNTARKVPAE
RVADYLAGYALANDVSLPESSFYRPAIKAKCRDGFCPLGDIGQLATSDRLEIITEINGVEQDRWSTADLVRSVPELIAAI
SDFITLQPGDAVLIGTPHQRVEIKPGDEVTVRAAGLPTLTNRVTQAGGAS

Specific function: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5- tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7- dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate) [H]

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI13654274, Length=180, Percent_Identity=30, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI215422413, Length=176, Percent_Identity=30.1136363636364, Blast_Score=78, Evalue=5e-15,
Organism=Homo sapiens, GI156231349, Length=190, Percent_Identity=31.0526315789474, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI66348062, Length=171, Percent_Identity=30.4093567251462, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI40786394, Length=190, Percent_Identity=31.0526315789474, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1787428, Length=183, Percent_Identity=37.1584699453552, Blast_Score=85, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17557057, Length=171, Percent_Identity=33.9181286549708, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI28572127, Length=217, Percent_Identity=29.4930875576037, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28571789, Length=181, Percent_Identity=30.939226519337, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24663695, Length=162, Percent_Identity=26.5432098765432, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012684
- InterPro:   IPR012686 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =5.3.3.10; =4.1.1.68 [H]

Molecular weight: Translated: 22526; Mature: 22526

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGTVFSVALNHRSQMDAWDQAFHQPPYKTPPKTPVWFIKPRNTHLANGGAIPFPAGEQV
CCCEEEEEEECCCHHCCHHHHHHCCCCCCCCCCCCEEEECCCCCEECCCCCCCCCCCCHH
QSGATLAVVIGNTARKVPAERVADYLAGYALANDVSLPESSFYRPAIKAKCRDGFCPLGD
HCCCEEEEEECCCHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCC
IGQLATSDRLEIITEINGVEQDRWSTADLVRSVPELIAAISDFITLQPGDAVLIGTPHQR
HHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCCE
VEIKPGDEVTVRAAGLPTLTNRVTQAGGAS
EEECCCCCEEEEECCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MKGTVFSVALNHRSQMDAWDQAFHQPPYKTPPKTPVWFIKPRNTHLANGGAIPFPAGEQV
CCCEEEEEEECCCHHCCHHHHHHCCCCCCCCCCCCEEEECCCCCEECCCCCCCCCCCCHH
QSGATLAVVIGNTARKVPAERVADYLAGYALANDVSLPESSFYRPAIKAKCRDGFCPLGD
HCCCEEEEEECCCHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCC
IGQLATSDRLEIITEINGVEQDRWSTADLVRSVPELIAAISDFITLQPGDAVLIGTPHQR
HHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCCE
VEIKPGDEVTVRAAGLPTLTNRVTQAGGAS
EEECCCCCEEEEECCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA