Click here to switch to the map view.

The map label for this gene is lpsJ [H]

Identifier: 157368836

GI number: 157368836

Start: 655534

End: 656187

Strand: Direct

Name: lpsJ [H]

Synonym: Spro_0591

Alternate gene names: 157368836

Gene position: 655534-656187 (Clockwise)

Preceding gene: 157368835

Following gene: 157368837

Centisome position: 12.03

GC content: 60.09

Gene sequence:

>654_bases
ATGCTTACCCGTGAACAAATGGCGCAGCGCGTGGCACAAGAACTGCGTGACGGTGACTACGTCAATCTGGGGATCGGCAT
TCCTACGCTGGTGGCCAACTATGTGCCGCCGGGCATGGACGTGATGCTGCAATCCGAAAACGGCCTGCTGGGAATGGGCG
GCTTCCCGACCGAAGCTGAGCTGGATGCCGACATGATCAACGCCGGTAAGCAGACGGTCACCGCCCGTATCGGCGCCTCG
ATTTTCGACTCGGCACAGTCGTTCGCCATGATCCGTGGCGGCCATGTCGATCTCACCGTATTGGGCGCTTTTGAAGTGGA
CGTCGAAGGCAACATCGCCTCGTGGATGATCCCCGGCAAGTTGGTGAAGGGCATGGGTGGCGCGATGGATCTGGTGGCCG
GTGCGGAGAACATCATTGTGGTGATGACCCACGCGTCAAAGAGTGGCGAGTCCAAGCTGTTACCGCGCTGCACGTTGCCG
TTGACCGGTGCCGGTTGCATTCGCAAGGTTCTGACCGATCTGGCCTATCTGGAAATTGAAAACGGTGCCTTTGTGCTGCG
CGAACGTGCGCCCGGCGTCAGCGTGGAGGAGATTGTCGCCAAAACCGCCGGTCGGCTGATAGTGCCGACTCAGGTGCCGG
AGATGCGCTTCTGA

Upstream 100 bases:

>100_bases
GCCGCGATCCACACCCCCGGCATCTACGTTGACCGCCTGATCCAGGGCAGTTTCGAAAAGCGCATTGAACAGCGCACTCT
GCGTACCTGAGGAGAACCCC

Downstream 100 bases:

>100_bases
TGATGTTATCCAGGGAGAAAACCATGCAACAGGTAGTGATTGTAGCGGCGACTCGCACGCCGATTGGCAGTTTTCAGGGC
GCGCTGGCCTCATTGTCGGC

Product: 3-oxoacid CoA-transferase subunit B

Products: NA

Alternate protein names: OXCT B; Succinyl CoA:3-oxoacid CoA-transferase [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MLTREQMAQRVAQELRDGDYVNLGIGIPTLVANYVPPGMDVMLQSENGLLGMGGFPTEAELDADMINAGKQTVTARIGAS
IFDSAQSFAMIRGGHVDLTVLGAFEVDVEGNIASWMIPGKLVKGMGGAMDLVAGAENIIVVMTHASKSGESKLLPRCTLP
LTGAGCIRKVLTDLAYLEIENGAFVLRERAPGVSVEEIVAKTAGRLIVPTQVPEMRF

Sequences:

>Translated_217_residues
MLTREQMAQRVAQELRDGDYVNLGIGIPTLVANYVPPGMDVMLQSENGLLGMGGFPTEAELDADMINAGKQTVTARIGAS
IFDSAQSFAMIRGGHVDLTVLGAFEVDVEGNIASWMIPGKLVKGMGGAMDLVAGAENIIVVMTHASKSGESKLLPRCTLP
LTGAGCIRKVLTDLAYLEIENGAFVLRERAPGVSVEEIVAKTAGRLIVPTQVPEMRF
>Mature_217_residues
MLTREQMAQRVAQELRDGDYVNLGIGIPTLVANYVPPGMDVMLQSENGLLGMGGFPTEAELDADMINAGKQTVTARIGAS
IFDSAQSFAMIRGGHVDLTVLGAFEVDVEGNIASWMIPGKLVKGMGGAMDLVAGAENIIVVMTHASKSGESKLLPRCTLP
LTGAGCIRKVLTDLAYLEIENGAFVLRERAPGVSVEEIVAKTAGRLIVPTQVPEMRF

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=215, Percent_Identity=48.8372093023256, Blast_Score=216, Evalue=1e-56,
Organism=Homo sapiens, GI11545841, Length=215, Percent_Identity=48.3720930232558, Blast_Score=210, Evalue=7e-55,
Organism=Escherichia coli, GI1788552, Length=216, Percent_Identity=49.0740740740741, Blast_Score=214, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17531517, Length=215, Percent_Identity=51.1627906976744, Blast_Score=211, Evalue=2e-55,
Organism=Drosophila melanogaster, GI21358325, Length=214, Percent_Identity=54.2056074766355, Blast_Score=234, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24655794, Length=214, Percent_Identity=54.2056074766355, Blast_Score=234, Evalue=3e-62,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.5 [H]

Molecular weight: Translated: 23031; Mature: 23031

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTREQMAQRVAQELRDGDYVNLGIGIPTLVANYVPPGMDVMLQSENGLLGMGGFPTEAE
CCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCCCEEEEECCCCEEECCCCCCCCC
LDADMINAGKQTVTARIGASIFDSAQSFAMIRGGHVDLTVLGAFEVDVEGNIASWMIPGK
CCHHHHHCCCCEEHHHHCHHHHHHHHHHHEEECCCEEEEEEEEEEEEECCCEEEEECCHH
LVKGMGGAMDLVAGAENIIVVMTHASKSGESKLLPRCTLPLTGAGCIRKVLTDLAYLEIE
HHHCCCCHHHHHCCCCCEEEEEEECCCCCCCCCCCEECCCCCCHHHHHHHHHHHHEEEEC
NGAFVLRERAPGVSVEEIVAKTAGRLIVPTQVPEMRF
CCEEEEEECCCCCCHHHHHHHHCCCEEEECCCCCCCC
>Mature Secondary Structure
MLTREQMAQRVAQELRDGDYVNLGIGIPTLVANYVPPGMDVMLQSENGLLGMGGFPTEAE
CCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCCCEEEEECCCCEEECCCCCCCCC
LDADMINAGKQTVTARIGASIFDSAQSFAMIRGGHVDLTVLGAFEVDVEGNIASWMIPGK
CCHHHHHCCCCEEHHHHCHHHHHHHHHHHEEECCCEEEEEEEEEEEEECCCEEEEECCHH
LVKGMGGAMDLVAGAENIIVVMTHASKSGESKLLPRCTLPLTGAGCIRKVLTDLAYLEIE
HHHCCCCHHHHHCCCCCEEEEEEECCCCCCCCCCCEECCCCCCHHHHHHHHHHHHEEEEC
NGAFVLRERAPGVSVEEIVAKTAGRLIVPTQVPEMRF
CCEEEEEECCCCCCHHHHHHHHCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9385134 [H]