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The map label for this gene is thiC [H]

Identifier: 157368533

GI number: 157368533

Start: 325689

End: 327632

Strand: Reverse

Name: thiC [H]

Synonym: Spro_0284

Alternate gene names: 157368533

Gene position: 327632-325689 (Counterclockwise)

Preceding gene: 157368534

Following gene: 157368532

Centisome position: 6.01

GC content: 58.28

Gene sequence:

>1944_bases
ATGTCTAACGTTAACAAACCCCGCGCTCGTAAAGAGCAACGCGAAGAAGCCCAGCAGTTTATCAACACCCTGCAAGGCGT
TTCCTTCCCCAATTCACGCCGTATCTATCTGCAGGGCTCACGTAGTGATCTGCAGGTGCCAATGCGTGAAATTCAGCTCA
GCCCGACACTGATCGGTGGCGATAAGGACAACCCGAAGTACGAGCCAAACGAAGCGATTCCGGTGTACGACACTGCCGGC
CCTTACGGTGATCCCCAATCAGAACTCAACGTGCATAGCGGCCTGGCGAAGCTACGCGCCGGTTGGATCGCTGAACGCGG
TGATACCGAAGCGCTCAAGGGTGTCAGTTCAGGTTTCACCCAGCAGCGCCTGGCCGATGAAGGCCTGGATCACCTGCGCT
TTGAACATTTGCCGTTGCCGCGCAAGGCACAACCGGGCCAATGCGTGACCCAGTTGCACTACGCGCGTGCTGGCACCGTC
ACCCCAGAAATGGAATTTATCGCCATTCGCGAAAACATGGGGCGCGAGCGCATCCGTGGCGAGGTATTACGCCACCAGCA
TCCTGGCCAAAGTTGGGGGGCCAACCTGCCGGAGAACATCACGCCGGAATTCGTTCGCCAGGAAGTCGCCGCCGGGCGCG
CCATCATCCCCGCCAATATCAACCACCCGGAATCCGAACCGATGATCATCGGCCGTAACTTCCTGGTGAAGGTTAACGCC
AACATCGGCAACTCGGCGGTGACCTCCTCGATCGAGGAAGAGGTCGAGAAACTGGTGTGGTCCACCCGCTGGGGCGCGGA
TACCGTGATGGATCTGTCTACCGGCCGCTATATCCACGAAACCCGCGAATGGATCCTGCGTAACAGCCCGGTGCCAATTG
GCACGGTGCCTATCTATCAGGCGCTGGAAAAGGTGAACGGCGTGGCGGAAAACCTCACCTGGGAAATGTTCCGCGATACG
CTGCTGGAGCAGGCGGAACAAGGGGTTGACTACTTCACCATTCACGCCGGCGTGCTGCTGCGCTATGTGCCGATGACCGC
CAAACGCCTGACCGGCATCGTTTCCCGCGGCGGTTCGATCATGGCCAAATGGTGCCTGTCACATCATCAGGAAAACTTCC
TGTACCAGCACTTCCGCGAAATCTGTGAGATTTGCGCCGCCTATGACGTTTCTCTTTCCCTCGGCGACGGCCTGCGGCCA
GGCTCAATTCAGGACGCCAACGACGAGGCACAGTTCGCCGAACTGCACACCTTGGGTGAGTTGACCAAAATTGCCTGGGA
ATATGATGTGCAGGTAATGATCGAAGGCCCTGGCCACGTGCCGATGCAGATGATCCGCCGCAACATGACCGAGGAGCTGG
AGCACTGCCACGAAGCACCATTCTATACTCTTGGCCCACTGACCACCGATATCGCTCCGGGTTATGACCACTTTACCTCC
GGCATCGGGGCCGCGATGATCGGCTGGTTCGGCTGCGCCATGCTGTGTTACGTCACGCCAAAGGAGCATCTCGGGCTGCC
GAACAAAGAGGACGTCAAGCAGGGGCTGATCACCTACAAAATTGCCGCTCACGCCGCAGACCTGGCAAAAGGCCACCCCG
GTGCGCAAATCCGCGATAACGCCATGTCCAAGGCGCGCTTCGAATTCCGCTGGGAGGATCAGTTCAATCTGGCGCTCGAT
CCAGCCACTGCTCGCGCTTATCACGACGAAACCCTGCCGCAAGAGTCCGGCAAAATCGCCCACTTCTGCTCTATGTGCGG
GCCAAAATTCTGCTCGATGAAAATTTCGCAGGAAGTTCGCGACTATGCCGCCGCGCAGGAAGCGGCCAAACCGATAGCAG
TGCAGTTAACCGGCATGGAAAAGATGTCGGCCGAGTTCCGCTCACGCGGCAGCGAGCTGTACCACAGCGCCGGTAACCTG
CAAGAGGAATTAAACAATGACTGA

Upstream 100 bases:

>100_bases
CCCGATTTGGGATAATCCCGCTTTGCCGGCCAGCATCATTACTCCCTCCGAGCTCCAGACAAGCAACTTTCCCAACCCAA
CACACTGGTAGGAACTTGCT

Downstream 100 bases:

>100_bases
TATCACCACGCCCTTCCCCGCCACGCCTCACAAGTTGGGGTTATACCCGGTGGTCGACAGCGTTGAATGGATTGCCCGTT
TGCTGGACGCAGGCGTCACT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 647; Mature: 646

Protein sequence:

>647_residues
MSNVNKPRARKEQREEAQQFINTLQGVSFPNSRRIYLQGSRSDLQVPMREIQLSPTLIGGDKDNPKYEPNEAIPVYDTAG
PYGDPQSELNVHSGLAKLRAGWIAERGDTEALKGVSSGFTQQRLADEGLDHLRFEHLPLPRKAQPGQCVTQLHYARAGTV
TPEMEFIAIRENMGRERIRGEVLRHQHPGQSWGANLPENITPEFVRQEVAAGRAIIPANINHPESEPMIIGRNFLVKVNA
NIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQALEKVNGVAENLTWEMFRDT
LLEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSLGDGLRP
GSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHFTS
GIGAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALD
PATARAYHDETLPQESGKIAHFCSMCGPKFCSMKISQEVRDYAAAQEAAKPIAVQLTGMEKMSAEFRSRGSELYHSAGNL
QEELNND

Sequences:

>Translated_647_residues
MSNVNKPRARKEQREEAQQFINTLQGVSFPNSRRIYLQGSRSDLQVPMREIQLSPTLIGGDKDNPKYEPNEAIPVYDTAG
PYGDPQSELNVHSGLAKLRAGWIAERGDTEALKGVSSGFTQQRLADEGLDHLRFEHLPLPRKAQPGQCVTQLHYARAGTV
TPEMEFIAIRENMGRERIRGEVLRHQHPGQSWGANLPENITPEFVRQEVAAGRAIIPANINHPESEPMIIGRNFLVKVNA
NIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQALEKVNGVAENLTWEMFRDT
LLEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSLGDGLRP
GSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHFTS
GIGAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALD
PATARAYHDETLPQESGKIAHFCSMCGPKFCSMKISQEVRDYAAAQEAAKPIAVQLTGMEKMSAEFRSRGSELYHSAGNL
QEELNND
>Mature_646_residues
SNVNKPRARKEQREEAQQFINTLQGVSFPNSRRIYLQGSRSDLQVPMREIQLSPTLIGGDKDNPKYEPNEAIPVYDTAGP
YGDPQSELNVHSGLAKLRAGWIAERGDTEALKGVSSGFTQQRLADEGLDHLRFEHLPLPRKAQPGQCVTQLHYARAGTVT
PEMEFIAIRENMGRERIRGEVLRHQHPGQSWGANLPENITPEFVRQEVAAGRAIIPANINHPESEPMIIGRNFLVKVNAN
IGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQALEKVNGVAENLTWEMFRDTL
LEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSLGDGLRPG
SIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHFTSG
IGAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDP
ATARAYHDETLPQESGKIAHFCSMCGPKFCSMKISQEVRDYAAAQEAAKPIAVQLTGMEKMSAEFRSRGSELYHSAGNLQ
EELNND

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=625, Percent_Identity=86.24, Blast_Score=1131, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 72347; Mature: 72215

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVNKPRARKEQREEAQQFINTLQGVSFPNSRRIYLQGSRSDLQVPMREIQLSPTLIGG
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHCCHHHHEECCEEECC
DKDNPKYEPNEAIPVYDTAGPYGDPQSELNVHSGLAKLRAGWIAERGDTEALKGVSSGFT
CCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHH
QQRLADEGLDHLRFEHLPLPRKAQPGQCVTQLHYARAGTVTPEMEFIAIRENMGRERIRG
HHHHHHHCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHEEEEHHHCCHHHHHH
EVLRHQHPGQSWGANLPENITPEFVRQEVAAGRAIIPANINHPESEPMIIGRNFLVKVNA
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCEEEEEEEC
NIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQ
CCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHH
ALEKVNGVAENLTWEMFRDTLLEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHH
MAKWCLSHHQENFLYQHFREICEICAAYDVSLSLGDGLRPGSIQDANDEAQFAELHTLGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
LTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHFTS
HHHHHEEECEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHH
GIGAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDN
HHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
AMSKARFEFRWEDQFNLALDPATARAYHDETLPQESGKIAHFCSMCGPKFCSMKISQEVR
HHHHCEEEEEECCCCCEEECCHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHH
DYAAAQEAAKPIAVQLTGMEKMSAEFRSRGSELYHSAGNLQEELNND
HHHHHHHHCCCEEEEEECHHHHHHHHHHCCHHHHHHHCCHHHHHCCC
>Mature Secondary Structure 
SNVNKPRARKEQREEAQQFINTLQGVSFPNSRRIYLQGSRSDLQVPMREIQLSPTLIGG
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHCCHHHHEECCEEECC
DKDNPKYEPNEAIPVYDTAGPYGDPQSELNVHSGLAKLRAGWIAERGDTEALKGVSSGFT
CCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHH
QQRLADEGLDHLRFEHLPLPRKAQPGQCVTQLHYARAGTVTPEMEFIAIRENMGRERIRG
HHHHHHHCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHEEEEHHHCCHHHHHH
EVLRHQHPGQSWGANLPENITPEFVRQEVAAGRAIIPANINHPESEPMIIGRNFLVKVNA
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCEEEEEEEC
NIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQ
CCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHH
ALEKVNGVAENLTWEMFRDTLLEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHH
MAKWCLSHHQENFLYQHFREICEICAAYDVSLSLGDGLRPGSIQDANDEAQFAELHTLGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
LTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHFTS
HHHHHEEECEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHH
GIGAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDN
HHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
AMSKARFEFRWEDQFNLALDPATARAYHDETLPQESGKIAHFCSMCGPKFCSMKISQEVR
HHHHCEEEEEECCCCCEEECCHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHH
DYAAAQEAAKPIAVQLTGMEKMSAEFRSRGSELYHSAGNLQEELNND
HHHHHHHHCCCEEEEEECHHHHHHHHHHCCHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]