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The map label for this gene is thiG

Identifier: 157368529

GI number: 157368529

Start: 323326

End: 324105

Strand: Reverse

Name: thiG

Synonym: Spro_0280

Alternate gene names: 157368529

Gene position: 324105-323326 (Counterclockwise)

Preceding gene: 157368530

Following gene: 157368528

Centisome position: 5.95

GC content: 61.41

Gene sequence:

>780_bases
ATGCTACAAATCGCCGATACCACTTTTACCTCACGCCTGTTTACCGGCACCGGTAAATTCGCCACCCCAACGCTGATGCT
GGAGGCATTACAGGCCTCCGGCTCGCAGCTGGTCACCATGGCAATGAAGCGTGTTGACCTGCGCGGCGGTAATGACGCCA
TTCTGGCGCCGTTGCAACAACTGGGTGTACGCCTGTTGCCGAATACTTCCGGCGCCAAGACCGCGGCAGAAGCGGTGTTC
GCCGCCCGGTTGGCCCGTGAGGCGCTTGGCACCCACTGGGTGAAACTGGAAATCCATCCCGATGTGAAATACCTGCTGCC
GGACCCGATCGAAACCCTGAAGGCGGCGGAAATATTGGTGAAAGAAGGTTTTGTGGTGCTGCCTTACTGCGGTGCCGATC
CGGTGTTGTGTAAACGCCTGGAGGAAGCGGGCTGTGCCGCGGTCATGCCGCTGGGCGCGCCTATTGGTTCCAACCGTGGG
CTGCGCACCCGCGACTTCCTGGAGATCATTATCGAGCAGGCCAGGGTGCCGGTGGTCGTCGACGCCGGCATCGGTGCGCC
AAGCCATGCGCTGGAAGCCATGGAACTGGGTGCCGATGCCGTGTTGGTGAATACCGCAATCGCCGTGGCACGCGACCCGG
TGCAGATGGCTCGGGCTTTTCGCCTGGCGCTTGAGGCCGGAGAATTGGCACGTCAGGCGGGATTGGGCAGCAGTCAGCGT
AATGCGGTTGCCTCCAGCCCGTTGACCGCTTTTCTCAGCCAGACAGGGGAGACATTGTGA

Upstream 100 bases:

>100_bases
GCTATCAACCAAATCATCATCCCGCGCACCGACTGGGCTAACCACCTGGTGCAAGACGGCGATGACATTCTGCTGTTTCA
AGCGATCGCCGGAGGCTGAC

Downstream 100 bases:

>100_bases
TGGCTGATGATTTCAGTAGCCGCTGGCAACAGTTGGATTGGGACGATATCTCGCTGCGTATTAACAGTAAAACGGCGCGT
GATGTCGAGCATGCGCTGAA

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MLQIADTTFTSRLFTGTGKFATPTLMLEALQASGSQLVTMAMKRVDLRGGNDAILAPLQQLGVRLLPNTSGAKTAAEAVF
AARLAREALGTHWVKLEIHPDVKYLLPDPIETLKAAEILVKEGFVVLPYCGADPVLCKRLEEAGCAAVMPLGAPIGSNRG
LRTRDFLEIIIEQARVPVVVDAGIGAPSHALEAMELGADAVLVNTAIAVARDPVQMARAFRLALEAGELARQAGLGSSQR
NAVASSPLTAFLSQTGETL

Sequences:

>Translated_259_residues
MLQIADTTFTSRLFTGTGKFATPTLMLEALQASGSQLVTMAMKRVDLRGGNDAILAPLQQLGVRLLPNTSGAKTAAEAVF
AARLAREALGTHWVKLEIHPDVKYLLPDPIETLKAAEILVKEGFVVLPYCGADPVLCKRLEEAGCAAVMPLGAPIGSNRG
LRTRDFLEIIIEQARVPVVVDAGIGAPSHALEAMELGADAVLVNTAIAVARDPVQMARAFRLALEAGELARQAGLGSSQR
NAVASSPLTAFLSQTGETL
>Mature_259_residues
MLQIADTTFTSRLFTGTGKFATPTLMLEALQASGSQLVTMAMKRVDLRGGNDAILAPLQQLGVRLLPNTSGAKTAAEAVF
AARLAREALGTHWVKLEIHPDVKYLLPDPIETLKAAEILVKEGFVVLPYCGADPVLCKRLEEAGCAAVMPLGAPIGSNRG
LRTRDFLEIIIEQARVPVVVDAGIGAPSHALEAMELGADAVLVNTAIAVARDPVQMARAFRLALEAGELARQAGLGSSQR
NAVASSPLTAFLSQTGETL

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=252, Percent_Identity=78.1746031746032, Blast_Score=394, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_SERP5 (A8G8F0)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001476518.1
- ProteinModelPortal:   A8G8F0
- SMR:   A8G8F0
- STRING:   A8G8F0
- GeneID:   5607020
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0280
- eggNOG:   COG2022
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   PRK00208
- BioCyc:   SPRO399741:SPRO_0280-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27277; Mature: 27277

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: ACT_SITE 95-95 BINDING 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQIADTTFTSRLFTGTGKFATPTLMLEALQASGSQLVTMAMKRVDLRGGNDAILAPLQQ
CEEECCCHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
LGVRLLPNTSGAKTAAEAVFAARLAREALGTHWVKLEIHPDVKYLLPDPIETLKAAEILV
CCEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEECCCHHHHHHHHHHHH
KEGFVVLPYCGADPVLCKRLEEAGCAAVMPLGAPIGSNRGLRTRDFLEIIIEQARVPVVV
HCCCEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEE
DAGIGAPSHALEAMELGADAVLVNTAIAVARDPVQMARAFRLALEAGELARQAGLGSSQR
ECCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NAVASSPLTAFLSQTGETL
CHHHCCHHHHHHHHCCCCC
>Mature Secondary Structure
MLQIADTTFTSRLFTGTGKFATPTLMLEALQASGSQLVTMAMKRVDLRGGNDAILAPLQQ
CEEECCCHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
LGVRLLPNTSGAKTAAEAVFAARLAREALGTHWVKLEIHPDVKYLLPDPIETLKAAEILV
CCEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEECCCHHHHHHHHHHHH
KEGFVVLPYCGADPVLCKRLEEAGCAAVMPLGAPIGSNRGLRTRDFLEIIIEQARVPVVV
HCCCEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEE
DAGIGAPSHALEAMELGADAVLVNTAIAVARDPVQMARAFRLALEAGELARQAGLGSSQR
ECCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NAVASSPLTAFLSQTGETL
CHHHCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA