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The map label for this gene is thiH [H]

Identifier: 157368528

GI number: 157368528

Start: 322193

End: 323326

Strand: Reverse

Name: thiH [H]

Synonym: Spro_0279

Alternate gene names: 157368528

Gene position: 323326-322193 (Counterclockwise)

Preceding gene: 157368529

Following gene: 157368518

Centisome position: 5.93

GC content: 58.47

Gene sequence:

>1134_bases
ATGGCTGATGATTTCAGTAGCCGCTGGCAACAGTTGGATTGGGACGATATCTCGCTGCGTATTAACAGTAAAACGGCGCG
TGATGTCGAGCATGCGCTGAATGCGGAAAAACTGTCGCGAGAAGACTTTATGGCACTGATTTCCCCTGCCGCCGCGTCCT
ACCTGGAGCCACTGGCGCAGCGTGCGCAGCAGATGACCCGTCAACGTTTTGGCAACGTGGTTAGCTTCTACGTGCCGCTG
TACCTGTCCAATCTGTGCGCCAACGACTGCACCTACTGCGGCTTTTCGATGAGCAACCGCATCAAGCGTAAAACCCTGGA
TGCCGCCGAGATCGAACGTGAGTGTCTGGCGATTAAGGCGTTGGGCTTTGAGCATTTGCTGTTGGTCACCGGGGAACACC
AGACCAAGGTCGGCATGGACTATTTCCGCCAACACATTCCAGCGATCCGCCGCCATTTCAGCTCGCTGATGATGGAAGTA
CAACCGCTGGAGCAGCAAGAGTACGCCGAATTAAAGGCTCTGGGGTTGGATGGCGTGCTGGTGTATCAGGAAACCTATCA
TCCCGCGACCTACCTGCAGCATCATCTGCGCGGTCAGAAACAGGACTTTCACTGGCGGTTGGCCACACCGGATCGCCTGG
GCCGCGCCGGGATCGACAAGATCGGCCTCGGGGCCCTGATCGGGCTTTCCAACAGTTGGCGTACCGACTGCTACATGCTG
GCTGAACACCTGTTCTACCTGCAACAGACTTACTGGCAGAGCCGCTACTCGATCTCGTTCCCGCGCCTGCGCCCTTGTGC
CGGAGGTATCGAACCGGCGTCGATCATGAGTGAACCGCAATTGGTGCAGCTAATCTGTGCCTTCCGGCTGTTCGCCCCCG
ACGTGGAGCTGTCTTTGTCGACGCGTGAGTCGCCGTTTTTCCGCGATCATATGATCCCGGTGGCGATCAACAGCGTCAGC
GCCGGCTCCAAAACCCAGCCTGGCGGCTATGCCGACGATGTGCCGCCGGAGCTGGAACAGTTTGAACCGCACGATGGCCG
TACCCCACAGCAGGTGGCACAAGCCATCAGCGACGCTGGTTTACAGCCGGTGTGGAAAGACTGGGACGGATACCTGGGGC
GCAGCCCGCAGTAA

Upstream 100 bases:

>100_bases
GAGAATTGGCACGTCAGGCGGGATTGGGCAGCAGTCAGCGTAATGCGGTTGCCTCCAGCCCGTTGACCGCTTTTCTCAGC
CAGACAGGGGAGACATTGTG

Downstream 100 bases:

>100_bases
TCTGCAAGCCTTGCAACGCCGTTGAAAAAACGAGGGAAGCGTTGCGCAGAAGCTATCGACATCGCTGGCGGGGCAAATCA
GCCCCGCTATAGTGGCGATG

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MADDFSSRWQQLDWDDISLRINSKTARDVEHALNAEKLSREDFMALISPAAASYLEPLAQRAQQMTRQRFGNVVSFYVPL
YLSNLCANDCTYCGFSMSNRIKRKTLDAAEIERECLAIKALGFEHLLLVTGEHQTKVGMDYFRQHIPAIRRHFSSLMMEV
QPLEQQEYAELKALGLDGVLVYQETYHPATYLQHHLRGQKQDFHWRLATPDRLGRAGIDKIGLGALIGLSNSWRTDCYML
AEHLFYLQQTYWQSRYSISFPRLRPCAGGIEPASIMSEPQLVQLICAFRLFAPDVELSLSTRESPFFRDHMIPVAINSVS
AGSKTQPGGYADDVPPELEQFEPHDGRTPQQVAQAISDAGLQPVWKDWDGYLGRSPQ

Sequences:

>Translated_377_residues
MADDFSSRWQQLDWDDISLRINSKTARDVEHALNAEKLSREDFMALISPAAASYLEPLAQRAQQMTRQRFGNVVSFYVPL
YLSNLCANDCTYCGFSMSNRIKRKTLDAAEIERECLAIKALGFEHLLLVTGEHQTKVGMDYFRQHIPAIRRHFSSLMMEV
QPLEQQEYAELKALGLDGVLVYQETYHPATYLQHHLRGQKQDFHWRLATPDRLGRAGIDKIGLGALIGLSNSWRTDCYML
AEHLFYLQQTYWQSRYSISFPRLRPCAGGIEPASIMSEPQLVQLICAFRLFAPDVELSLSTRESPFFRDHMIPVAINSVS
AGSKTQPGGYADDVPPELEQFEPHDGRTPQQVAQAISDAGLQPVWKDWDGYLGRSPQ
>Mature_376_residues
ADDFSSRWQQLDWDDISLRINSKTARDVEHALNAEKLSREDFMALISPAAASYLEPLAQRAQQMTRQRFGNVVSFYVPLY
LSNLCANDCTYCGFSMSNRIKRKTLDAAEIERECLAIKALGFEHLLLVTGEHQTKVGMDYFRQHIPAIRRHFSSLMMEVQ
PLEQQEYAELKALGLDGVLVYQETYHPATYLQHHLRGQKQDFHWRLATPDRLGRAGIDKIGLGALIGLSNSWRTDCYMLA
EHLFYLQQTYWQSRYSISFPRLRPCAGGIEPASIMSEPQLVQLICAFRLFAPDVELSLSTRESPFFRDHMIPVAINSVSA
GSKTQPGGYADDVPPELEQFEPHDGRTPQQVAQAISDAGLQPVWKDWDGYLGRSPQ

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=373, Percent_Identity=77.7479892761394, Blast_Score=616, Evalue=1e-178,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 42891; Mature: 42760

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADDFSSRWQQLDWDDISLRINSKTARDVEHALNAEKLSREDFMALISPAAASYLEPLAQ
CCCCHHHHHHCCCCCCEEEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
RAQQMTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKRKTLDAAEIERECLAIKA
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
LGFEHLLLVTGEHQTKVGMDYFRQHIPAIRRHFSSLMMEVQPLEQQEYAELKALGLDGVL
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEE
VYQETYHPATYLQHHLRGQKQDFHWRLATPDRLGRAGIDKIGLGALIGLSNSWRTDCYML
EEECCCCHHHHHHHHHCCCCCCCEEEECCHHHHCCCCCCHHCCHHHEECCCCCCHHHHHH
AEHLFYLQQTYWQSRYSISFPRLRPCAGGIEPASIMSEPQLVQLICAFRLFAPDVELSLS
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCEEEEC
TRESPFFRDHMIPVAINSVSAGSKTQPGGYADDVPPELEQFEPHDGRTPQQVAQAISDAG
CCCCCCHHHCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHCC
LQPVWKDWDGYLGRSPQ
CCCHHHCCCCCCCCCCC
>Mature Secondary Structure 
ADDFSSRWQQLDWDDISLRINSKTARDVEHALNAEKLSREDFMALISPAAASYLEPLAQ
CCCHHHHHHCCCCCCEEEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
RAQQMTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKRKTLDAAEIERECLAIKA
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
LGFEHLLLVTGEHQTKVGMDYFRQHIPAIRRHFSSLMMEVQPLEQQEYAELKALGLDGVL
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEE
VYQETYHPATYLQHHLRGQKQDFHWRLATPDRLGRAGIDKIGLGALIGLSNSWRTDCYML
EEECCCCHHHHHHHHHCCCCCCCEEEECCHHHHCCCCCCHHCCHHHEECCCCCCHHHHHH
AEHLFYLQQTYWQSRYSISFPRLRPCAGGIEPASIMSEPQLVQLICAFRLFAPDVELSLS
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCEEEEC
TRESPFFRDHMIPVAINSVSAGSKTQPGGYADDVPPELEQFEPHDGRTPQQVAQAISDAG
CCCCCCHHHCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHCC
LQPVWKDWDGYLGRSPQ
CCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]