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The map label for this gene is uidB

Identifier: 157161077

GI number: 157161077

Start: 1714843

End: 1716216

Strand: Reverse

Name: uidB

Synonym: EcHS_A1691

Alternate gene names: 157161077

Gene position: 1716216-1714843 (Counterclockwise)

Preceding gene: 157161078

Following gene: 157161076

Centisome position: 36.96

GC content: 51.89

Gene sequence:

>1374_bases
ATGAATCAACAACTCTCCTGGCGCACCATCGTCGGCTACAGCCTCGGTGACGTCGCCAATAACTTCGCCTTCGCAATGGG
GGCGCTCTTCCTGTTGAGTTACTACACCGACGTCGCTGGCGTCGGTGCCGCTGCGGCGGGCACCATGCTGTTACTGGTGC
GGGTATTCGATGCCTTCGCCGACGTCTTTGCCGGACGAGTGGTGGACAGTGTGAATACCCGCTGGGGAAAATTCCGCCCG
TTTTTACTCTTCGGTACTGCGCCGTTAATGATCTTCAGCGTGCTGGTATTCTGGGTGCCGACCGACTGGAGCCATGGTAG
CAAAGTGGTGTATGCATATTTGACCTACATGGGCCTCGGGCTTTGCTACAGCCTGGTGAATATTCCTTATGGTTCACTTG
CTACCGCGATGACCCAACAACCACAATCCCGCGCCCGTCTGGGCGCGGCTCGTGGGATTGCCGCTTCATTGACCTTTGTC
TGCCTGGCATTTCTGATAGGACCGAGCATTAAGAACTCCAGCCCGGAAGAGATGGTGTCGGTATACCATTTCTGGACAAT
TGTGCTGGCGATTGCCGGAATGGTGCTTTACTTCATCTGCTTCAAATCGACGCGTGAGAATGTGGTACGTATCGTTGCGC
AGCCGTCATTGAATATCAGTCTGCAAACCCTGAAACGGAATCGCCCGCTGTTTATGTTGTGCATCGGTGCGCTGTGTGTG
CTGATTTCGACCTTTGCGGTCAGCGCCTCGTCGTTGTTCTACGTGCGCTATGTGTTAAATGATACCGGGCTGTTCACTGT
GCTGGTACTGGTGCAAAACCTGGTTGGTACTGTGGCATCGGCACCGCTGGTGCCGGGGATGGTCGCGAGGATCGGTAAAA
AGAATACCTTCCTGATTGGCGCTTTGCTGGGAACCTGCGGTTATCTGCTGTTCTTCTGGGTTTCCGTCTGGTCACTGCCG
GTGGCGTTGGTTGCGTTGGCCATCGCTTCAATTGGTCAGGGCGTTACCATGACCGTGATGTGGGCGCTGGAAGCTGATAC
CGTAGAATACGGTGAATACCTGACCGGCGTGCGAATTGAAGGGCTCACCTATTCACTATTCTCATTTACCCGTAAATGCG
GTCAGGCAATCGGAGGTTCAATTCCTGCCTTTATTTTGGGGTTAAGCGGATATATCGCCAATCAGGTGCAAACGCCGGAA
GTTATTATGGGCATCCGCACATCAATTGCCTTAGTACCTTGCGGATTTATGCTACTGGCATTCGTTATTATCTGGTTTTA
TCCGCTCACGGATAAAAAATTCAAAGAAATCGTGGTTGAAATTGATAATCGTAAAAAAGTGCAGCAGCAATTAATCAGCG
ATATCACTAATTAA

Upstream 100 bases:

>100_bases
TCTTCACTCGCGACCGCAAACCGAAGTCGGCGGCTTTTCTGCTGCAAAAACGCTGGACTGGCATGAACTTCGGTGAAAAA
CCGCAGCAGGGAGGCAAACA

Downstream 100 bases:

>100_bases
TATTCAATAAAAATAATCAGAACATCAAAGGTGCAACTATGAGAAAAATAGTGGCCATGGCCGTTATTTGCCTGACGGCT
GCCTCTGGCCTTACCTCTGC

Product: glucuronide transporter

Products: Proton [Cytoplasm]; Glucuronides [Cytoplasm] [C]

Alternate protein names: Glucuronide permease

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAAAAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRP
FLLFGTAPLMIFSVLVFWVPTDWSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLNISLQTLKRNRPLFMLCIGALCV
LISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVGTVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLP
VALVALAIASIGQGVTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYIANQVQTPE
VIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQLISDITN

Sequences:

>Translated_457_residues
MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAAAAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRP
FLLFGTAPLMIFSVLVFWVPTDWSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLNISLQTLKRNRPLFMLCIGALCV
LISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVGTVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLP
VALVALAIASIGQGVTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYIANQVQTPE
VIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQLISDITN
>Mature_457_residues
MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAAAAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRP
FLLFGTAPLMIFSVLVFWVPTDWSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLNISLQTLKRNRPLFMLCIGALCV
LISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVGTVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLP
VALVALAIASIGQGVTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYIANQVQTPE
VIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQLISDITN

Specific function: Responsible for the transport of glucuronide into the cell energized by the proton motive force (probably by symport). Import is enhanced by uidC (gusC, AC Q47706)

COG id: COG2211

COG function: function code G; Na+/melibiose symporter and related transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:galactoside symporter (TC 2.A.2) family

Homologues:

Organism=Escherichia coli, GI1787902, Length=457, Percent_Identity=99.781181619256, Blast_Score=918, Evalue=0.0,
Organism=Escherichia coli, GI145693206, Length=453, Percent_Identity=33.112582781457, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI48994989, Length=457, Percent_Identity=32.6039387308534, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1786466, Length=460, Percent_Identity=31.0869565217391, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI87082306, Length=460, Percent_Identity=28.2608695652174, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1790561, Length=458, Percent_Identity=27.5109170305677, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UIDB2_ECOLI (P0CE45)

Other databases:

- EMBL:   M14641
- ProteinModelPortal:   P0CE45
- eggNOG:   COG2211
- HOGENOM:   HBG518114
- OMA:   PRITHSS
- Genevestigator:   P30868
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001927
- InterPro:   IPR018043
- TIGRFAMs:   TIGR00792

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 49893; Mature: 49893

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS00872 NA_GALACTOSIDE_SYMP; PS00527 RIBOSOMAL_S14

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x11a8031c)-; HASH(0x14ee0de4)-; HASH(0x1463f95c)-; HASH(0x14a8cd28)-; HASH(0x14aa4108)-; HASH(0x1491780c)-; HASH(0x14eb9fd0)-; HASH(0x1455bc94)-; HASH(0x14e88a0c)-; HASH(0x14e2e69c)-; HASH(0x14bb5b9c)-; HASH(0x1423c274)-;

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHCH
>Mature Secondary Structure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HHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; Glucuronides [Periplasm] [C]

Specific reaction: Proton [Periplasm] + Glucuronides [Periplasm] = Proton [Cytoplasm] + Glucuronides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3534890