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The map label for this gene is uidA [H]

Identifier: 157161078

GI number: 157161078

Start: 1716213

End: 1718024

Strand: Reverse

Name: uidA [H]

Synonym: EcHS_A1692

Alternate gene names: 157161078

Gene position: 1718024-1716213 (Counterclockwise)

Preceding gene: 157161079

Following gene: 157161077

Centisome position: 37.0

GC content: 52.15

Gene sequence:

>1812_bases
ATGTTACGTCCTGTAGAAACCCCAACCCGTGAAATCAAAAAACTCGACGGCCTGTGGGCATTCAGTCTGGATCGCGAAAA
CTGTGGAATTGATCAGCGTTGGTGGGAAAGCGCGTTACAAGAAAGCCGGGCAATTGCTGTGCCAGGCAGTTTTAACGATC
AGTTCGCCGATGCAGATATTCGTAATTATGCGGGCAACGTCTGGTATCAGCGCGAAGTCTTTATACCGAAAGGTTGGGCA
GGCCAGCGTATCGTGCTGCGTTTCGATGCGGTCACTCATTACGGCAAAGTGTGGGTCAATAATCAGGAAGTGATGGAGCA
TCAGGGCGGCTATACGCCATTTGAAGCCGATGTCACGCCGTATGTTATTGCCGGGAAAAGTGTACGTATCACCGTTTGTG
TGAACAACGAACTGAACTGGCAGACTATCCCGCCGGGAATGGTGATTACCGACGAAAACGGCAAGAAAAAGCAGTCTTAC
TTCCATGATTTCTTTAACTATGCCGGGATCCATCGCAGCGTAATGCTCTACACCACGCCGAACACCTGGGTGGACGATAT
CACCGTGGTGACGCATGTCGCGCAAGACTGTAACCACGCGTCTGTTGACTGGCAGGTGGTGACCAATGGTGATGTCAGCG
TTGAACTGCGTGATGCGGATCAACAGGTGGTTGCAACTGGACAAGGCACTAGCGGGACTTTGCAAGTGGTGAATCCGCAC
CTCTGGCAACCGGGTGAAGGTTATCTCTATGAACTGTGCGTCACAGCCAAAAGCCAGACAGAGTATGATATCTACCCGCT
TCGCGTCGGCATCCGGTCAGTGGCAGTGAAGGGCGAACAGTTCCTGATTAACCACAAACCGTTCTACTTTACGGGTTTTG
GTCGTCATGAAGATGCGGACTTGCGTGGCAAAGGATTCGATAACGTGCTGATGGTGCACGACCACGCATTAATGGACTGG
ATTGGGGCCAACTCCTACCGTACCTCGCATTACCCTTACGCTGAAGAGATGCTCGACTGGGCAGATGAACATGGCATCGT
GGTGATTGATGAAACTGCTGCTGTCGGCTTTAACCTCTCTTTAGGCATTGGTTTCGAAGCGGGCAACAAGCCGAAAGAAC
TGTACAGCGAAGAGGCAGTCAACGGGGAAACTCAGCAAGCGCACTTACAGGCGATTAAAGAGCTGATAGCGCGTGACAAA
AACCACCCAAGCGTGGTGATGTGGAGTATTGCCAACGAACCGGATACCCGTCCGCAAGGTGCACGGGAATATTTCGCGCC
ACTGGCGGAAGCAACGCGTAAACTCGACCCGACGCGTCCGATCACCTGCGTCAATGTAATGTTCTGCGACGCTCACACCG
ATACCATCAGCGATCTCTTTGATGTGCTGTGCCTGAACCGTTATTACGGATGGTATGTCCAAAGCGGCGATTTGGAAACG
GCAGAGAAGGTACTGGAAAAAGAACTTCTGGCCTGGCAGGAGAAACTGCATCAGCCGATTATCATCACCGAATACGGCGT
GGATACGTTAGCCGGGCTGCACTCAATGTACACCGACATGTGGAGTGAAGAGTATCAGTGTGCATGGCTGGATATGTATC
ACCGCGTCTTTGATCGCGTCAGCGCCGTCGTCGGTGAACAGGTATGGAATTTCGCCGATTTTGCGACCTCGCAAGGCATA
TTGCGCGTTGGCGGTAACAAGAAAGGGATCTTCACTCGCGACCGCAAACCGAAGTCGGCGGCTTTTCTGCTGCAAAAACG
CTGGACTGGCATGAACTTCGGTGAAAAACCGCAGCAGGGAGGCAAACAATGA

Upstream 100 bases:

>100_bases
CTTCCATGGGTTTCTCACAGATAACTGTGTGCAACACAGAATTGGTTAACTAATCAGATTAAAGGTTGACCAGTATTATT
ATCTTAATGAGGAGTCCCTT

Downstream 100 bases:

>100_bases
ATCAACAACTCTCCTGGCGCACCATCGTCGGCTACAGCCTCGGTGACGTCGCCAATAACTTCGCCTTCGCAATGGGGGCG
CTCTTCCTGTTGAGTTACTA

Product: beta-D-glucuronidase

Products: NA

Alternate protein names: GUS; Beta-D-glucuronoside glucuronosohydrolase [H]

Number of amino acids: Translated: 603; Mature: 603

Protein sequence:

>603_residues
MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWA
GQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVTNGDVSVELRDADQQVVATGQGTSGTLQVVNPH
LWQPGEGYLYELCVTAKSQTEYDIYPLRVGIRSVAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDW
IGANSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK
NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLET
AEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGI
LRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ

Sequences:

>Translated_603_residues
MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWA
GQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVTNGDVSVELRDADQQVVATGQGTSGTLQVVNPH
LWQPGEGYLYELCVTAKSQTEYDIYPLRVGIRSVAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDW
IGANSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK
NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLET
AEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGI
LRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ
>Mature_603_residues
MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWA
GQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVTNGDVSVELRDADQQVVATGQGTSGTLQVVNPH
LWQPGEGYLYELCVTAKSQTEYDIYPLRVGIRSVAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDW
IGANSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK
NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLET
AEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGI
LRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ

Specific function: Unknown

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=636, Percent_Identity=44.9685534591195, Blast_Score=505, Evalue=1e-143,
Organism=Escherichia coli, GI1787903, Length=603, Percent_Identity=99.6683250414594, Blast_Score=1258, Evalue=0.0,
Organism=Escherichia coli, GI1786539, Length=484, Percent_Identity=26.6528925619835, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI48994920, Length=392, Percent_Identity=25.5102040816327, Blast_Score=105, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17510825, Length=617, Percent_Identity=36.4667747163695, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI19922582, Length=624, Percent_Identity=43.2692307692308, Blast_Score=483, Evalue=1e-136,
Organism=Drosophila melanogaster, GI62471735, Length=624, Percent_Identity=43.2692307692308, Blast_Score=483, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24655438, Length=624, Percent_Identity=43.2692307692308, Blast_Score=483, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24651745, Length=633, Percent_Identity=39.652448657188, Blast_Score=437, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.31 [H]

Molecular weight: Translated: 68538; Mature: 68538

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00719 GLYCOSYL_HYDROL_F2_1 ; PS00608 GLYCOSYL_HYDROL_F2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIAVPGSFNDQFADADI
CCCCCCCCHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCHH
RNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTP
HHCCCCEEEEEEEECCCCCCCCEEEEEEECHHCCCEEEECCHHHHHHCCCCCCCCCCCCC
YVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDFFNYAGIHRSVMLYTTP
EEEECCEEEEEEEECCCCCCEECCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECC
NTWVDDITVVTHVAQDCNHASVDWQVVTNGDVSVELRDADQQVVATGQGTSGTLQVVNPH
CCCHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCEEEEECCCCCCEEEEECCC
LWQPGEGYLYELCVTAKSQTEYDIYPLRVGIRSVAVKGEQFLINHKPFYFTGFGRHEDAD
CCCCCCCEEEEEEEECCCCCCCEEEEEEECHHHHEECCCEEEEECCCEEEECCCCCCCCC
LRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFNLS
CCCCCCCCEEEEECHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCEEEEEEE
LGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQG
EEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCC
AREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLET
HHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCEEEECCCHHH
AEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRV
HHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
SAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQG
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCCHHHHHHHHHCCCCCCCCCCCCC
GKQ
CCC
>Mature Secondary Structure
MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIAVPGSFNDQFADADI
CCCCCCCCHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCHH
RNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTP
HHCCCCEEEEEEEECCCCCCCCEEEEEEECHHCCCEEEECCHHHHHHCCCCCCCCCCCCC
YVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDFFNYAGIHRSVMLYTTP
EEEECCEEEEEEEECCCCCCEECCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECC
NTWVDDITVVTHVAQDCNHASVDWQVVTNGDVSVELRDADQQVVATGQGTSGTLQVVNPH
CCCHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCEEEEECCCCCCEEEEECCC
LWQPGEGYLYELCVTAKSQTEYDIYPLRVGIRSVAVKGEQFLINHKPFYFTGFGRHEDAD
CCCCCCCEEEEEEEECCCCCCCEEEEEEECHHHHEECCCEEEEECCCEEEECCCCCCCCC
LRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFNLS
CCCCCCCCEEEEECHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCEEEEEEE
LGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQG
EEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCC
AREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLET
HHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCEEEECCCHHH
AEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRV
HHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
SAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQG
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCCHHHHHHHHHCCCCCCCCCCCCC
GKQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3534890; 8125312; 2103475; 9097039; 9278503 [H]