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The map label for this gene is spaS [H]

Identifier: 157159225

GI number: 157159225

Start: 3197269

End: 3198000

Strand: Reverse

Name: spaS [H]

Synonym: EcE24377A_3181

Alternate gene names: 157159225

Gene position: 3198000-3197269 (Counterclockwise)

Preceding gene: 157158728

Following gene: 157155332

Centisome position: 64.22

GC content: 32.51

Gene sequence:

>732_bases
ATGGCCAATAAAACTGAAAAGCCAACACAAAAGAAACTCCAGGATGCTTCTAAAAAAGGGCAAATTCTAAAAAGTAGAGA
CTTAACCATCTCTGTAATAATGCTTGTGGGTACTTTATATCTCGGATATGTTTTTGATGTGCATCACATTATGTCGATTC
TTGAATACATCCTTGATCATAACGCTAAACCAGATATTTGGGACTATTTTAAAGCTATGGGGGTTGGTTGGTTGAAAACG
AACATTCCTTTTTTGCTGGTTTGCATGTTCACAACAATACTTGTCTCCTGGTTTCAAAGTAAAATGCAATTAGCAACTGA
AGCTGTTAAATTTAAGTTTGATTCATTGAATCCAGTAAATGGCTTAAAGCGTATATTTGGCTTAAAAACCGTAAAAGAAT
TTGTTAAAGCAATTCTTTATATTGTTTTTTTTGCATTGGCGATCAAAGTATTTTGGAGTAATCATAAATCACTGCTTTTT
AAAACTCTTGATGGAGATATCATATCTTTATTATCAGATTGGGGAGAGATGCTATTCCTTCTCATACTGTATTGTCTCGG
CAGTATGATAATTGTCTTAATTTTTGATTTTATCGCTGAATATTTTTTATTTATGAAAGATATGAAAATGGATAAACAAG
AAGTTAAAAGAGAATACAAGGAGCAAGAAGGAAATCCAGAAATTAAGTCTAAACGCAGAGAGCGCATCAGGAAATTCTTT
CTGAGCAATTGA

Upstream 100 bases:

>100_bases
TATTAATTATTTATTTTGCCCCAATACTGGCTGAACGAGTAATGCCGTTATCCTTTTTCCCAGAACAATTGCAACTATAC
ATTGAGAAATAGAGTGTTTC

Downstream 100 bases:

>100_bases
AGTCTGATGTCAGTAATAGTCGTTTGATGATTTCCAACCCTACTCACATTGCAATAGGATATACTTTAAGCCACATCTTT
CACCTATTCCATTGATTTCT

Product: FlhB/HrpN/YscU/SpaS family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MANKTEKPTQKKLQDASKKGQILKSRDLTISVIMLVGTLYLGYVFDVHHIMSILEYILDHNAKPDIWDYFKAMGVGWLKT
NIPFLLVCMFTTILVSWFQSKMQLATEAVKFKFDSLNPVNGLKRIFGLKTVKEFVKAILYIVFFALAIKVFWSNHKSLLF
KTLDGDIISLLSDWGEMLFLLILYCLGSMIIVLIFDFIAEYFLFMKDMKMDKQEVKREYKEQEGNPEIKSKRRERIRKFF
LSN

Sequences:

>Translated_243_residues
MANKTEKPTQKKLQDASKKGQILKSRDLTISVIMLVGTLYLGYVFDVHHIMSILEYILDHNAKPDIWDYFKAMGVGWLKT
NIPFLLVCMFTTILVSWFQSKMQLATEAVKFKFDSLNPVNGLKRIFGLKTVKEFVKAILYIVFFALAIKVFWSNHKSLLF
KTLDGDIISLLSDWGEMLFLLILYCLGSMIIVLIFDFIAEYFLFMKDMKMDKQEVKREYKEQEGNPEIKSKRRERIRKFF
LSN
>Mature_242_residues
ANKTEKPTQKKLQDASKKGQILKSRDLTISVIMLVGTLYLGYVFDVHHIMSILEYILDHNAKPDIWDYFKAMGVGWLKTN
IPFLLVCMFTTILVSWFQSKMQLATEAVKFKFDSLNPVNGLKRIFGLKTVKEFVKAILYIVFFALAIKVFWSNHKSLLFK
TLDGDIISLLSDWGEMLFLLILYCLGSMIIVLIFDFIAEYFLFMKDMKMDKQEVKREYKEQEGNPEIKSKRRERIRKFFL
SN

Specific function: Involved in a secretory pathway responsible for the surface presentation of determinants needed for the entry of Salmonella species into mammalian cells [H]

COG id: COG1377

COG function: function code NU; Flagellar biosynthesis pathway, component FlhB

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type III secretion exporter family [H]

Homologues:

Organism=Escherichia coli, GI1788188, Length=237, Percent_Identity=26.5822784810127, Blast_Score=91, Evalue=5e-20,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006135
- InterPro:   IPR006307 [H]

Pfam domain/function: PF01312 Bac_export_2 [H]

EC number: NA

Molecular weight: Translated: 28472; Mature: 28341

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCC
>Mature Secondary Structure 
ANKTEKPTQKKLQDASKKGQILKSRDLTISVIMLVGTLYLGYVFDVHHIMSILEYILDH
CCCCCCHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NAKPDIWDYFKAMGVGWLKTNIPFLLVCMFTTILVSWFQSKMQLATEAVKFKFDSLNPVN
CCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
GLKRIFGLKTVKEFVKAILYIVFFALAIKVFWSNHKSLLFKTLDGDIISLLSDWGEMLFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LILYCLGSMIIVLIFDFIAEYFLFMKDMKMDKQEVKREYKEQEGNPEIKSKRRERIRKFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LSN
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8404849; 11677609 [H]