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The map label for this gene is rimK [H]

Identifier: 156977938

GI number: 156977938

Start: 1866048

End: 1866908

Strand: Direct

Name: rimK [H]

Synonym: VIBHAR_06734

Alternate gene names: 156977938

Gene position: 1866048-1866908 (Clockwise)

Preceding gene: 156977936

Following gene: 156977939

Centisome position: 84.67

GC content: 46.69

Gene sequence:

>861_bases
ATGCGCCTAAAGCAGGCGGGTGAAGAGCGCGGACATCAAGTTGATGTTATCGATACTCTGCACTGTTATATGGATATTAC
GAGTAACAACCCGAAGATTCGTTACAAGGGTGAAGAATTGCCAAAGTATGACGCGGTTATTCCACGCATCGGTGCATCTA
TCACTTTCTACGGTACAGCGGTTGTACGTCAATTTGAAATGATGGGCACATTCTGTGTAAACGAGTCAGTAGCAATCAGC
CGCTCTCGTGACAAGCTACGCTCTATGCAGCTGCTATCGCGCAAAGGCATTGGTCTACCTCGCACAGGTTTTGCTCACCA
TCCTGACAACATTCAGGACGTGATCAAAAACGTTGGCGGTGCACCTTTGGTTATCAAGCTACTTGAAGGTACTCAAGGTA
TCGGCGTTGTACTTGCTGAAACAACAAAAGCGGCAGAAAGCGTAATCGAAGCATTCATGGGTCTAAAAGCGAACATCATG
GTGCAAGAGTTCATTGAAGAAGCAAATGGTGCTGATATCCGCTGCTTCGTTGTAGGTAACAAAGTTATCGCAGCAATGAA
GCGTCAAGCTAAAGAAGGTGAGTTCCGTTCTAACCTTCACCGTGGTGGTTCTGCTGAGCTAGTAAAACTAACCAAAGAAG
AGCGCGCAACCGCGATCAACGCAGCAAAAGTCATGGGGCTAAACCTATGTGGTGTTGATATTCTTCAATCGAAAAACGGC
CCTGTTGTGATGGAAGTGAACTCTTCTCCAGGTCTTGAAGGCATCGAAACTGCAACAGAAAAAGACGTTGCAGGCATGAT
CTTCGACTTCATCGAGAAGAACGCAAAACCAAACGCTAACCGTACACGCGGTAAAGGCTGA

Upstream 100 bases:

>100_bases
CACTATACTCTATGTTTATATTTTTAATTTGGTACTGACTTACTAAGGAATTGCCATGCGTATTGCTATTCTTTCTCGTA
ATGAGAATCTATATTCAACC

Downstream 100 bases:

>100_bases
TAATTGTTTGAGTGAGAGCCTATAAACCGGGCTCTCTTTATCACTCATCGCTAGGATGACACTATGAATCAGAAAATGAT
CATTGGGAACGCCGAAGCAA

Product: glutathione synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MRLKQAGEERGHQVDVIDTLHCYMDITSNNPKIRYKGEELPKYDAVIPRIGASITFYGTAVVRQFEMMGTFCVNESVAIS
RSRDKLRSMQLLSRKGIGLPRTGFAHHPDNIQDVIKNVGGAPLVIKLLEGTQGIGVVLAETTKAAESVIEAFMGLKANIM
VQEFIEEANGADIRCFVVGNKVIAAMKRQAKEGEFRSNLHRGGSAELVKLTKEERATAINAAKVMGLNLCGVDILQSKNG
PVVMEVNSSPGLEGIETATEKDVAGMIFDFIEKNAKPNANRTRGKG

Sequences:

>Translated_286_residues
MRLKQAGEERGHQVDVIDTLHCYMDITSNNPKIRYKGEELPKYDAVIPRIGASITFYGTAVVRQFEMMGTFCVNESVAIS
RSRDKLRSMQLLSRKGIGLPRTGFAHHPDNIQDVIKNVGGAPLVIKLLEGTQGIGVVLAETTKAAESVIEAFMGLKANIM
VQEFIEEANGADIRCFVVGNKVIAAMKRQAKEGEFRSNLHRGGSAELVKLTKEERATAINAAKVMGLNLCGVDILQSKNG
PVVMEVNSSPGLEGIETATEKDVAGMIFDFIEKNAKPNANRTRGKG
>Mature_286_residues
MRLKQAGEERGHQVDVIDTLHCYMDITSNNPKIRYKGEELPKYDAVIPRIGASITFYGTAVVRQFEMMGTFCVNESVAIS
RSRDKLRSMQLLSRKGIGLPRTGFAHHPDNIQDVIKNVGGAPLVIKLLEGTQGIGVVLAETTKAAESVIEAFMGLKANIM
VQEFIEEANGADIRCFVVGNKVIAAMKRQAKEGEFRSNLHRGGSAELVKLTKEERATAINAAKVMGLNLCGVDILQSKNG
PVVMEVNSSPGLEGIETATEKDVAGMIFDFIEKNAKPNANRTRGKG

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6 [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI134254462, Length=279, Percent_Identity=27.5985663082437, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI164663810, Length=215, Percent_Identity=31.1627906976744, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1787076, Length=274, Percent_Identity=62.7737226277372, Blast_Score=369, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 31131; Mature: 31131

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLKQAGEERGHQVDVIDTLHCYMDITSNNPKIRYKGEELPKYDAVIPRIGASITFYGTA
CCCCHHHHHCCCEEEHEEEEEEEEEEECCCCEEEECCCCCCCHHHHHHHCCCEEEEHHHH
VVRQFEMMGTFCVNESVAISRSRDKLRSMQLLSRKGIGLPRTGFAHHPDNIQDVIKNVGG
HHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCC
APLVIKLLEGTQGIGVVLAETTKAAESVIEAFMGLKANIMVQEFIEEANGADIRCFVVGN
CCEEEEEECCCCCCEEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEECC
KVIAAMKRQAKEGEFRSNLHRGGSAELVKLTKEERATAINAAKVMGLNLCGVDILQSKNG
HHHHHHHHHHHCCHHHHHHCCCCCHHHEEHHHHHHHHHHHHHHHHCCEECCHHHEECCCC
PVVMEVNSSPGLEGIETATEKDVAGMIFDFIEKNAKPNANRTRGKG
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MRLKQAGEERGHQVDVIDTLHCYMDITSNNPKIRYKGEELPKYDAVIPRIGASITFYGTA
CCCCHHHHHCCCEEEHEEEEEEEEEEECCCCEEEECCCCCCCHHHHHHHCCCEEEEHHHH
VVRQFEMMGTFCVNESVAISRSRDKLRSMQLLSRKGIGLPRTGFAHHPDNIQDVIKNVGG
HHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCC
APLVIKLLEGTQGIGVVLAETTKAAESVIEAFMGLKANIMVQEFIEEANGADIRCFVVGN
CCEEEEEECCCCCCEEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEECC
KVIAAMKRQAKEGEFRSNLHRGGSAELVKLTKEERATAINAAKVMGLNLCGVDILQSKNG
HHHHHHHHHHHCCHHHHHHCCCCCHHHEEHHHHHHHHHHHHHHHHCCEECCHHHEECCCC
PVVMEVNSSPGLEGIETATEKDVAGMIFDFIEKNAKPNANRTRGKG
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]