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The map label for this gene is carB3 [H]

Identifier: 156977582

GI number: 156977582

Start: 1544367

End: 1545218

Strand: Direct

Name: carB3 [H]

Synonym: VIBHAR_06370

Alternate gene names: 156977582

Gene position: 1544367-1545218 (Clockwise)

Preceding gene: 156977581

Following gene: 156977583

Centisome position: 70.07

GC content: 47.07

Gene sequence:

>852_bases
ATGAAAAAGCTATTTCTACTACTTGGACTGTTAGCCTGTTCAAGTGCAACGTACGCCGCCAAACTTAATGAAGACATCTC
CGCGATTGAAGAGCATATTTCGGGTCGAATCGGAGTGTCTGTCTGGAATACTCAAACCGATGATCATTGGGACTATAGAG
GCGACGAGCGCTTCCCGATGATGAGCACATTCAAAACGCTTGCATGTGCAACGATGCTTAGTGACATGGATAATGAAAAG
CTCGACAAAAACGCCACTGCCAAAGTAGAAGAGCGCAATATGGTGGTTTGGTCACCAGTAATGGATCGTATGGCAGGTCA
AACAACGCGTATTGAACATGCTTGTGAAGCCACAATGCTGATGAGCGACAATACCGCAGCCAACATTGTTTTGCGTAGCA
TTGGTGGACCACGCGGTGTCACCACGTTCTTGCGTTCTGTCGGTGATAAAGCAACGCGCCTTGACCGTTTTGAACCAAGA
CTGAACGAGGCGAACCCAGGCGACAAGCGAGACACCACAACACCTAACGCCATGGTAAATACACTGCACACGTTGTTAGA
AGGTGATGCGCTATCCTACGAATCACGCATTCAATTAAAGATCTGGATGCAGGATAACAAGGTCTCTGATTCTTTGATGC
GCTCTGTGTTACCAAAGGGTTGGTCGATTGCCGATCGTTCTGGCGCAGGGGGCTTTGGGTCACGCGGCATCACAGCAATG
ATCTGGAAAGAGAATCATAAACCGATCTACATAAGCATTTACATTACTGAAACCGATTTATCCTTGCAGGCGCGCGATCA
GATTATCGCTCAAGTGAGCCAGTTAATACTGGATGAGTACAATACTATTTAG

Upstream 100 bases:

>100_bases
AAAGACAATAATCTAACAGTCATTAGAGTATTAACTCGCTATAGTTCGACTACATTCGCCTGAGTGTGAACCGAAATAAA
TAACACTAAGAAAATCAATA

Downstream 100 bases:

>100_bases
CACTCGCTAAATACTAAAAAACGGAGCTTTTCGAGCTCCGTTTTTTGTGGCGGGTAGCCTTACCAGTAAGCAGGACTATC
CAAACCTTTCACGCAAGAGG

Product: beta-lactamase

Products: NA

Alternate protein names: Carbenicillinase 3 [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKKLFLLLGLLACSSATYAAKLNEDISAIEEHISGRIGVSVWNTQTDDHWDYRGDERFPMMSTFKTLACATMLSDMDNEK
LDKNATAKVEERNMVVWSPVMDRMAGQTTRIEHACEATMLMSDNTAANIVLRSIGGPRGVTTFLRSVGDKATRLDRFEPR
LNEANPGDKRDTTTPNAMVNTLHTLLEGDALSYESRIQLKIWMQDNKVSDSLMRSVLPKGWSIADRSGAGGFGSRGITAM
IWKENHKPIYISIYITETDLSLQARDQIIAQVSQLILDEYNTI

Sequences:

>Translated_283_residues
MKKLFLLLGLLACSSATYAAKLNEDISAIEEHISGRIGVSVWNTQTDDHWDYRGDERFPMMSTFKTLACATMLSDMDNEK
LDKNATAKVEERNMVVWSPVMDRMAGQTTRIEHACEATMLMSDNTAANIVLRSIGGPRGVTTFLRSVGDKATRLDRFEPR
LNEANPGDKRDTTTPNAMVNTLHTLLEGDALSYESRIQLKIWMQDNKVSDSLMRSVLPKGWSIADRSGAGGFGSRGITAM
IWKENHKPIYISIYITETDLSLQARDQIIAQVSQLILDEYNTI
>Mature_283_residues
MKKLFLLLGLLACSSATYAAKLNEDISAIEEHISGRIGVSVWNTQTDDHWDYRGDERFPMMSTFKTLACATMLSDMDNEK
LDKNATAKVEERNMVVWSPVMDRMAGQTTRIEHACEATMLMSDNTAANIVLRSIGGPRGVTTFLRSVGDKATRLDRFEPR
LNEANPGDKRDTTTPNAMVNTLHTLLEGDALSYESRIQLKIWMQDNKVSDSLMRSVLPKGWSIADRSGAGGFGSRGITAM
IWKENHKPIYISIYITETDLSLQARDQIIAQVSQLILDEYNTI

Specific function: Hydrolyzes both carbenicillin and oxacillin [H]

COG id: COG2367

COG function: function code V; Beta-lactamase class A

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-A beta-lactamase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR000871 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: =3.5.2.6 [H]

Molecular weight: Translated: 31657; Mature: 31657

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00146 BETA_LACTAMASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLFLLLGLLACSSATYAAKLNEDISAIEEHISGRIGVSVWNTQTDDHWDYRGDERFPM
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCH
MSTFKTLACATMLSDMDNEKLDKNATAKVEERNMVVWSPVMDRMAGQTTRIEHACEATML
HHHHHHHHHHHHHHHCCCHHCCCCCCCEEECCCEEEEHHHHHHHCCCCHHHHHHHHEEEE
MSDNTAANIVLRSIGGPRGVTTFLRSVGDKATRLDRFEPRLNEANPGDKRDTTTPNAMVN
ECCCCHHHHHEECCCCCHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
TLHTLLEGDALSYESRIQLKIWMQDNKVSDSLMRSVLPKGWSIADRSGAGGFGSRGITAM
HHHHHHCCCCCCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEE
IWKENHKPIYISIYITETDLSLQARDQIIAQVSQLILDEYNTI
EEECCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKLFLLLGLLACSSATYAAKLNEDISAIEEHISGRIGVSVWNTQTDDHWDYRGDERFPM
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCH
MSTFKTLACATMLSDMDNEKLDKNATAKVEERNMVVWSPVMDRMAGQTTRIEHACEATML
HHHHHHHHHHHHHHHCCCHHCCCCCCCEEECCCEEEEHHHHHHHCCCCHHHHHHHHEEEE
MSDNTAANIVLRSIGGPRGVTTFLRSVGDKATRLDRFEPRLNEANPGDKRDTTTPNAMVN
ECCCCHHHHHEECCCCCHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
TLHTLLEGDALSYESRIQLKIWMQDNKVSDSLMRSVLPKGWSIADRSGAGGFGSRGITAM
HHHHHHCCCCCCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEE
IWKENHKPIYISIYITETDLSLQARDQIIAQVSQLILDEYNTI
EEECCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1650733 [H]