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The map label for this gene is fruR [H]

Identifier: 156977171

GI number: 156977171

Start: 1143626

End: 1144621

Strand: Reverse

Name: fruR [H]

Synonym: VIBHAR_05957

Alternate gene names: 156977171

Gene position: 1144621-1143626 (Counterclockwise)

Preceding gene: 156977175

Following gene: 156977169

Centisome position: 51.93

GC content: 47.09

Gene sequence:

>996_bases
ATGACACTGGATGAAATAGCAAAATTGGCTGGGGTTTCCAAGACCACGGCAAGCTATGTGATTAACGGTAAAGCGCAGAA
ATACCGCATCAGCGAAAAGACTCAAAAGAAAGTCATGGCGGTGGTTGAGCATTATAATTACAAACCGGATCACGCCGCTT
CTTCTTTGAGAGCGGGCAACTCACGCTCGTTTGGTTTGATCATTCCAGACTTAGAAAACACCAGTTACGCACGTTTAGCA
AAACTGATTGAACAGAACTCTCGTAAAGCAGGCTATCAAATTTTGATTGGTTGCTCAGATGACAATCCGCAAACCGAAGA
GAAGGTCGCTGAGGCGCTAGTTAGCCGCCGCATTGATGCGTTGTTTGTTGCCAGTAGTATGCCTGCCGCCAGTGAGTACT
ACTTAAAGCTGCAAAACTCGGGCACACCCGTTATCGCACTCGACCGTCCTATGGATGATGAACACTTTTGTTGTGTGATC
AGTGAAGATTTCGATGCGGCATTTGAGCTGACAGAATCGGTACTGTCTTCGGAGATTGAATCCATCGGTCTGATTGGCGC
ATTAGAAGAACTTCAAGTTTCTAAAGAGCGTGAACTGGGTTTCCGCTCTGCTTGCCAGCAAGAAGGTAATGAGGCTTTGG
TCGGTTATGGCCAGCATTTCTCCCGTGAAGAAGGGAAGAAGGTGCTGGAAGATTGGATAGCAAAAGGCCAGCTACCTGAT
GCGGTAGTGACCATGTCATACACCTTATTAGAAGGCGTGTTGGACGTGATGTTAGAAAAGCCTGAACTGATGGGTAAAGT
GCGTTTGGCTACCTTTGGTGATAACCGCTTGCTTGACTTTCTGCCAATCAAAATCAACTCGCTACCACAGCAGTTTGAGC
TGATTGCTGACAGCGCTATGGAGCTGGCTCTTAATGCTTCAGCAAAACGCTATAAAGCAGGGGTGGAGTTGATTCCGCGT
AAGATCGTGAAACGTCACAGCGACGCTAAAGCTTAA

Upstream 100 bases:

>100_bases
CTTTCAGCTTTTATACTGAATCGGTTCAGCATATAATTCAACTTATCGAAGGATCGTTGATGTATTAATATGATCCCGCG
CACAGAACAGGGCTTAGAAT

Downstream 100 bases:

>100_bases
TTTCTACGGTCAGCGCCACAAAGGCGTGGTCGCTGGCGTTTTTATCTCTCTCAAAAGACGGGTTAATTAGGTGTGTATCC
AGCACTTGATAATTCGTCAC

Product: DNA-binding transcriptional regulator FruR

Products: NA

Alternate protein names: Catabolite repressor/activator [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTLDEIAKLAGVSKTTASYVINGKAQKYRISEKTQKKVMAVVEHYNYKPDHAASSLRAGNSRSFGLIIPDLENTSYARLA
KLIEQNSRKAGYQILIGCSDDNPQTEEKVAEALVSRRIDALFVASSMPAASEYYLKLQNSGTPVIALDRPMDDEHFCCVI
SEDFDAAFELTESVLSSEIESIGLIGALEELQVSKERELGFRSACQQEGNEALVGYGQHFSREEGKKVLEDWIAKGQLPD
AVVTMSYTLLEGVLDVMLEKPELMGKVRLATFGDNRLLDFLPIKINSLPQQFELIADSAMELALNASAKRYKAGVELIPR
KIVKRHSDAKA

Sequences:

>Translated_331_residues
MTLDEIAKLAGVSKTTASYVINGKAQKYRISEKTQKKVMAVVEHYNYKPDHAASSLRAGNSRSFGLIIPDLENTSYARLA
KLIEQNSRKAGYQILIGCSDDNPQTEEKVAEALVSRRIDALFVASSMPAASEYYLKLQNSGTPVIALDRPMDDEHFCCVI
SEDFDAAFELTESVLSSEIESIGLIGALEELQVSKERELGFRSACQQEGNEALVGYGQHFSREEGKKVLEDWIAKGQLPD
AVVTMSYTLLEGVLDVMLEKPELMGKVRLATFGDNRLLDFLPIKINSLPQQFELIADSAMELALNASAKRYKAGVELIPR
KIVKRHSDAKA
>Mature_330_residues
TLDEIAKLAGVSKTTASYVINGKAQKYRISEKTQKKVMAVVEHYNYKPDHAASSLRAGNSRSFGLIIPDLENTSYARLAK
LIEQNSRKAGYQILIGCSDDNPQTEEKVAEALVSRRIDALFVASSMPAASEYYLKLQNSGTPVIALDRPMDDEHFCCVIS
EDFDAAFELTESVLSSEIESIGLIGALEELQVSKERELGFRSACQQEGNEALVGYGQHFSREEGKKVLEDWIAKGQLPDA
VVTMSYTLLEGVLDVMLEKPELMGKVRLATFGDNRLLDFLPIKINSLPQQFELIADSAMELALNASAKRYKAGVELIPRK
IVKRHSDAKA

Specific function: Represses some operons but activates others. It is implicated in the regulation of a large number of operons encoding enzymes which comprise central pathways of carbon metabolism. Binds D-fructose as an inducer [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786268, Length=328, Percent_Identity=44.2073170731707, Blast_Score=291, Evalue=5e-80,
Organism=Escherichia coli, GI1789202, Length=278, Percent_Identity=25.1798561151079, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787948, Length=335, Percent_Identity=22.6865671641791, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI48994940, Length=212, Percent_Identity=25.9433962264151, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1789068, Length=267, Percent_Identity=23.9700374531835, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790194, Length=310, Percent_Identity=22.5806451612903, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788474, Length=340, Percent_Identity=23.8235294117647, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790369, Length=258, Percent_Identity=23.2558139534884, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787580, Length=291, Percent_Identity=22.3367697594502, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787906, Length=202, Percent_Identity=27.7227722772277, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790715, Length=182, Percent_Identity=26.3736263736264, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012781
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36598; Mature: 36467

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDEIAKLAGVSKTTASYVINGKAQKYRISEKTQKKVMAVVEHYNYKPDHAASSLRAGN
CCHHHHHHHHCCCHHHHHEEECCCCCEEECHHHHHHHHHHHHHHCCCCCCHHHHHHCCCC
SRSFGLIIPDLENTSYARLAKLIEQNSRKAGYQILIGCSDDNPQTEEKVAEALVSRRIDA
CCCCEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHH
LFVASSMPAASEYYLKLQNSGTPVIALDRPMDDEHFCCVISEDFDAAFELTESVLSSEIE
HEEECCCCCCCEEEEEEECCCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHH
SIGLIGALEELQVSKERELGFRSACQQEGNEALVGYGQHFSREEGKKVLEDWIAKGQLPD
HHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEECCHHCCHHHHHHHHHHHHHCCCCCC
AVVTMSYTLLEGVLDVMLEKPELMGKVRLATFGDNRLLDFLPIKINSLPQQFELIADSAM
HHHHHHHHHHHHHHHHHHCCHHHHCEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHH
ELALNASAKRYKAGVELIPRKIVKRHSDAKA
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TLDEIAKLAGVSKTTASYVINGKAQKYRISEKTQKKVMAVVEHYNYKPDHAASSLRAGN
CHHHHHHHHCCCHHHHHEEECCCCCEEECHHHHHHHHHHHHHHCCCCCCHHHHHHCCCC
SRSFGLIIPDLENTSYARLAKLIEQNSRKAGYQILIGCSDDNPQTEEKVAEALVSRRIDA
CCCCEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHH
LFVASSMPAASEYYLKLQNSGTPVIALDRPMDDEHFCCVISEDFDAAFELTESVLSSEIE
HEEECCCCCCCEEEEEEECCCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHH
SIGLIGALEELQVSKERELGFRSACQQEGNEALVGYGQHFSREEGKKVLEDWIAKGQLPD
HHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEECCHHCCHHHHHHHHHHHHHCCCCCC
AVVTMSYTLLEGVLDVMLEKPELMGKVRLATFGDNRLLDFLPIKINSLPQQFELIADSAM
HHHHHHHHHHHHHHHHHHCCHHHHCEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHH
ELALNASAKRYKAGVELIPRKIVKRHSDAKA
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]