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The map label for this gene is ycdT [H]

Identifier: 156976945

GI number: 156976945

Start: 932281

End: 933462

Strand: Reverse

Name: ycdT [H]

Synonym: VIBHAR_05730

Alternate gene names: 156976945

Gene position: 933462-932281 (Counterclockwise)

Preceding gene: 156976955

Following gene: 156976943

Centisome position: 42.35

GC content: 45.26

Gene sequence:

>1182_bases
ATGGTCAGTTTGGGTATGGATAACATCATTCAGATGACTCAATCTAACTGGGGAATTGCTGCAAACTTGCCCTACATTTT
GCTCGGTTGTGCGTTGTTGATGTGTTATTCGTTCAAGCAAGGACGAGAGTCCATGGTGTGTATTGCCATGTTGCTCGCCT
ACTTTGTGATTCAACATCGTTTACAGGTACCACTACAAACTGGCACGGCGCTTTTAGAGCTCACTATTTTAGCAAGCCTC
CTTCCCGTTTCGTTCTTGGCCGTGTTCCTCTTCAATAAAGAGGGCTTCGATTCCAAAGCGACACTCACTTATGTACTTAT
GTTGAGTATGTTTGTGTTTTGGTCTTACGTGACCTTGACCTACTATGTTGAAGGTGGGTTTGAGGAGTGGACCAATGGCA
TCTTGTTTATTGTTCCAACCGTTTCAAAGTTACCATTGGTTTTAGTGCTGTATTGCATTGGCATTGTGTGCTTTTTGGGC
ATTTTGGTCTTAAAGAAAAACAAGATCATTCATGCCATGACATACTCAGCCATGGTATTGGCATCAGCGACGTTTATCTT
CTTCGATGTTCAATACATTTCGAGCACCATGTTTACCCTTACGGGTTTGCTGATCATCATCTACTCGACATCAGCCAGCC
ACCAACTGGCTTTCACCGACAGTTTGACGCTCATCCCTAGCCGTCGCGCATTGGATCTCGACCTTAAGCATATGGGACGC
AAGTTTACTATCGCGATGCTGGATGTTGACCACTTTAAGAAGTTCAACGATACCTATGGCCACGATACCGGCGATGATGT
GCTCAAAATGGTCGCTTCTCGATTGATGAAGACACAAGGCGGCGCAAAGGCTTACCGATTTGGTGGAGAAGAGTTCACCA
TCGTGTTTAAAGGTAAATACACCGAAGATTGCGAAGCGCATTTAGAAGAAGTTCGTGAGCTTATTGCTCAGTATGACATG
ATTCTACGTGACCAAGACAACCGTCCTGACGACAATAAACAAGGACGCAAAAAACGCGGTAACGCAGAATCGAAGCAGAA
AACGGTATCTGTAACAGCCAGTTTTGGTGTTGCGGATAGCCGTAGCGCGCGTAACCCTGCTGATGTGATTAAGCTGGCAG
ATGATGCTCTATATAAAGCAAAAAAAGCGGGCCGTAACTGTGTGAAGTACTGCCACGCGTAA

Upstream 100 bases:

>100_bases
TAGATATATTTAACTATTTTCAGGTAATTTGCAGTAGATGGCATCATCTTTAATGACCTCCCCAGGCTTCCGCTTCCTAT
TCCCAGTCTTGTTATTGGGC

Downstream 100 bases:

>100_bases
TTCTTTCTTCCCAATTCTTTAAAGCTTGTTTTTTGGTTCTAAGACTTCTCAAGCCTTACCTTAGAAAAACACCCTAAACA
AGTGACAGTGTCACTATGAT

Product: sensory transduction protein kinase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MVSLGMDNIIQMTQSNWGIAANLPYILLGCALLMCYSFKQGRESMVCIAMLLAYFVIQHRLQVPLQTGTALLELTILASL
LPVSFLAVFLFNKEGFDSKATLTYVLMLSMFVFWSYVTLTYYVEGGFEEWTNGILFIVPTVSKLPLVLVLYCIGIVCFLG
ILVLKKNKIIHAMTYSAMVLASATFIFFDVQYISSTMFTLTGLLIIIYSTSASHQLAFTDSLTLIPSRRALDLDLKHMGR
KFTIAMLDVDHFKKFNDTYGHDTGDDVLKMVASRLMKTQGGAKAYRFGGEEFTIVFKGKYTEDCEAHLEEVRELIAQYDM
ILRDQDNRPDDNKQGRKKRGNAESKQKTVSVTASFGVADSRSARNPADVIKLADDALYKAKKAGRNCVKYCHA

Sequences:

>Translated_393_residues
MVSLGMDNIIQMTQSNWGIAANLPYILLGCALLMCYSFKQGRESMVCIAMLLAYFVIQHRLQVPLQTGTALLELTILASL
LPVSFLAVFLFNKEGFDSKATLTYVLMLSMFVFWSYVTLTYYVEGGFEEWTNGILFIVPTVSKLPLVLVLYCIGIVCFLG
ILVLKKNKIIHAMTYSAMVLASATFIFFDVQYISSTMFTLTGLLIIIYSTSASHQLAFTDSLTLIPSRRALDLDLKHMGR
KFTIAMLDVDHFKKFNDTYGHDTGDDVLKMVASRLMKTQGGAKAYRFGGEEFTIVFKGKYTEDCEAHLEEVRELIAQYDM
ILRDQDNRPDDNKQGRKKRGNAESKQKTVSVTASFGVADSRSARNPADVIKLADDALYKAKKAGRNCVKYCHA
>Mature_393_residues
MVSLGMDNIIQMTQSNWGIAANLPYILLGCALLMCYSFKQGRESMVCIAMLLAYFVIQHRLQVPLQTGTALLELTILASL
LPVSFLAVFLFNKEGFDSKATLTYVLMLSMFVFWSYVTLTYYVEGGFEEWTNGILFIVPTVSKLPLVLVLYCIGIVCFLG
ILVLKKNKIIHAMTYSAMVLASATFIFFDVQYISSTMFTLTGLLIIIYSTSASHQLAFTDSLTLIPSRRALDLDLKHMGR
KFTIAMLDVDHFKKFNDTYGHDTGDDVLKMVASRLMKTQGGAKAYRFGGEEFTIVFKGKYTEDCEAHLEEVRELIAQYDM
ILRDQDNRPDDNKQGRKKRGNAESKQKTVSVTASFGVADSRSARNPADVIKLADDALYKAKKAGRNCVKYCHA

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=221, Percent_Identity=28.9592760180996, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787056, Length=173, Percent_Identity=31.7919075144509, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1787816, Length=176, Percent_Identity=34.6590909090909, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI145693134, Length=209, Percent_Identity=26.7942583732057, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI87082007, Length=182, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081977, Length=107, Percent_Identity=36.4485981308411, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 44070; Mature: 44070

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSLGMDNIIQMTQSNWGIAANLPYILLGCALLMCYSFKQGRESMVCIAMLLAYFVIQHR
CCCCCHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LQVPLQTGTALLELTILASLLPVSFLAVFLFNKEGFDSKATLTYVLMLSMFVFWSYVTLT
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YYVEGGFEEWTNGILFIVPTVSKLPLVLVLYCIGIVCFLGILVLKKNKIIHAMTYSAMVL
EEEECCHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHH
ASATFIFFDVQYISSTMFTLTGLLIIIYSTSASHQLAFTDSLTLIPSRRALDLDLKHMGR
HHHHHHEEEHHHHHHHHHHHHHHHHEEEECCCCCEEEEECCEEECCCCCCEECCHHHCCC
KFTIAMLDVDHFKKFNDTYGHDTGDDVLKMVASRLMKTQGGAKAYRFGGEEFTIVFKGKY
EEEEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEECCC
TEDCEAHLEEVRELIAQYDMILRDQDNRPDDNKQGRKKRGNAESKQKTVSVTASFGVADS
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCHHHHEEEEEEECCCCCC
RSARNPADVIKLADDALYKAKKAGRNCVKYCHA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVSLGMDNIIQMTQSNWGIAANLPYILLGCALLMCYSFKQGRESMVCIAMLLAYFVIQHR
CCCCCHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LQVPLQTGTALLELTILASLLPVSFLAVFLFNKEGFDSKATLTYVLMLSMFVFWSYVTLT
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YYVEGGFEEWTNGILFIVPTVSKLPLVLVLYCIGIVCFLGILVLKKNKIIHAMTYSAMVL
EEEECCHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHH
ASATFIFFDVQYISSTMFTLTGLLIIIYSTSASHQLAFTDSLTLIPSRRALDLDLKHMGR
HHHHHHEEEHHHHHHHHHHHHHHHHEEEECCCCCEEEEECCEEECCCCCCEECCHHHCCC
KFTIAMLDVDHFKKFNDTYGHDTGDDVLKMVASRLMKTQGGAKAYRFGGEEFTIVFKGKY
EEEEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEECCC
TEDCEAHLEEVRELIAQYDMILRDQDNRPDDNKQGRKKRGNAESKQKTVSVTASFGVADS
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCHHHHEEEEEEECCCCCC
RSARNPADVIKLADDALYKAKKAGRNCVKYCHA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]