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The map label for this gene is mdh [H]

Identifier: 156938050

GI number: 156938050

Start: 1125184

End: 1126119

Strand: Reverse

Name: mdh [H]

Synonym: Igni_1263

Alternate gene names: 156938050

Gene position: 1126119-1125184 (Counterclockwise)

Preceding gene: 156938052

Following gene: 156938048

Centisome position: 86.79

GC content: 57.37

Gene sequence:

>936_bases
TTGGCAAGGGTCCCGTACAAGGTAGCCGTTATAGGAACCGGGAGGGTAGGCGCGACCTTCGCTTATACTATGGCTATAGT
GCCCGGCGTCGCCCGGATGGTCTTGGTCGACGCCGTTCCGGGCCTCAGCAAGGGCGTCATGGAAGACATCAAACACGCCG
CAGCGGTCTTCCGGAGGAGCATTCAAGTGGAGGCCTACGACGACGTGTCCAAAGTGGAGAACGCGGACGCGATCGTAATA
ACTGCCGGAAAGCCTAGGAAGGCCGATATGAGCAGAAGAGACTTAGCAAAGGTCAATGCTCAAATAATAAGGGATATAGG
CGACAAGCTTAGGGACAGGAACCCCGGAGCTTTCTACATGGTAATCACCAACCCCGTCGACGTTATGACCATGATACTGA
GCGACGTAATAGGTAACAAGGGAACCGTGATAGGCACCGGGACCAGCTTGGACACCTATAGGTTTAGGAGCGCGGTCAGC
GAGCTGCTTAACGAGCCGATAGCCGCCATAGACGGCTACGTGGTCGGAGAACACGGAGAGGAGGCCTTCGTGGCTTGGAG
TACAGTCACAGTGAAGGGCGTACCCATTGATGAGTACATAAAAGAAAAAGGGCTGGACTTGAGCAGGTCGAGGATAGAGG
AGTACGTGAAGGAGGTCGCGGCGACGATCATTGCCAAACAGGGGGCGACCATATGGGGCCCCGCGGCGACGTTCCAAGAG
ATAGTCGTGTCGCACTTGGCCAACGAGGGCAGAATAATACCGGTCTCCGTGGTTCAAGAGGTCCCCGGAGTGGGGAGGGT
GGCGGTCTCGGTTCCCACCAAGATCTCCGGGAGGCTGGTCCCGCTGCCCCAGCTGCTCAACGAGGAGGAGAGGGAGAGGC
TGAAGAGGGCGGCCGAGGCCATAAAGAGGGTATACGAGGAGGCCGTCTCAAGCTAA

Upstream 100 bases:

>100_bases
AGGCCGGGAGCCGGTAGGGGTCGAACACCCCTACCCGGCCTTGTATATACTTGTAAAACTCGTATAAGCTGACTCGAGCC
TCCCTTTCCGGGCGAGCAAG

Downstream 100 bases:

>100_bases
GGGCCTGAGGGATCCGACCAACGCGTGGACGAACAGTATTACAGTGATCAATTGGAACGCGTCCGCTACGGAGGAAAGCC
TCCTAACCCTTTTTACCTCG

Product: malate dehydrogenase (NAD)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MARVPYKVAVIGTGRVGATFAYTMAIVPGVARMVLVDAVPGLSKGVMEDIKHAAAVFRRSIQVEAYDDVSKVENADAIVI
TAGKPRKADMSRRDLAKVNAQIIRDIGDKLRDRNPGAFYMVITNPVDVMTMILSDVIGNKGTVIGTGTSLDTYRFRSAVS
ELLNEPIAAIDGYVVGEHGEEAFVAWSTVTVKGVPIDEYIKEKGLDLSRSRIEEYVKEVAATIIAKQGATIWGPAATFQE
IVVSHLANEGRIIPVSVVQEVPGVGRVAVSVPTKISGRLVPLPQLLNEEERERLKRAAEAIKRVYEEAVSS

Sequences:

>Translated_311_residues
MARVPYKVAVIGTGRVGATFAYTMAIVPGVARMVLVDAVPGLSKGVMEDIKHAAAVFRRSIQVEAYDDVSKVENADAIVI
TAGKPRKADMSRRDLAKVNAQIIRDIGDKLRDRNPGAFYMVITNPVDVMTMILSDVIGNKGTVIGTGTSLDTYRFRSAVS
ELLNEPIAAIDGYVVGEHGEEAFVAWSTVTVKGVPIDEYIKEKGLDLSRSRIEEYVKEVAATIIAKQGATIWGPAATFQE
IVVSHLANEGRIIPVSVVQEVPGVGRVAVSVPTKISGRLVPLPQLLNEEERERLKRAAEAIKRVYEEAVSS
>Mature_310_residues
ARVPYKVAVIGTGRVGATFAYTMAIVPGVARMVLVDAVPGLSKGVMEDIKHAAAVFRRSIQVEAYDDVSKVENADAIVIT
AGKPRKADMSRRDLAKVNAQIIRDIGDKLRDRNPGAFYMVITNPVDVMTMILSDVIGNKGTVIGTGTSLDTYRFRSAVSE
LLNEPIAAIDGYVVGEHGEEAFVAWSTVTVKGVPIDEYIKEKGLDLSRSRIEEYVKEVAATIIAKQGATIWGPAATFQEI
VVSHLANEGRIIPVSVVQEVPGVGRVAVSVPTKISGRLVPLPQLLNEEERERLKRAAEAIKRVYEEAVSS

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI291575128, Length=315, Percent_Identity=30.1587301587302, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4557032, Length=315, Percent_Identity=30.1587301587302, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI47059044, Length=309, Percent_Identity=28.1553398058252, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI221136809, Length=309, Percent_Identity=28.1553398058252, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI5031857, Length=309, Percent_Identity=28.4789644012945, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI260099723, Length=309, Percent_Identity=28.4789644012945, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI9257228, Length=320, Percent_Identity=29.0625, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI4504973, Length=320, Percent_Identity=29.0625, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI15082234, Length=308, Percent_Identity=28.5714285714286, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI260099727, Length=221, Percent_Identity=28.9592760180996, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI260099725, Length=248, Percent_Identity=29.0322580645161, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI207028494, Length=231, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI21735621, Length=233, Percent_Identity=30.9012875536481, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1789632, Length=182, Percent_Identity=29.6703296703297, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17535107, Length=293, Percent_Identity=30.7167235494881, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17554310, Length=178, Percent_Identity=35.3932584269663, Blast_Score=81, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6322765, Length=168, Percent_Identity=32.7380952380952, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17136226, Length=308, Percent_Identity=28.2467532467532, Blast_Score=120, Evalue=8e-28,
Organism=Drosophila melanogaster, GI45550422, Length=304, Percent_Identity=27.6315789473684, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24647881, Length=326, Percent_Identity=26.6871165644172, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24663599, Length=180, Percent_Identity=31.6666666666667, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 33641; Mature: 33510

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS00579 RIBOSOMAL_L29

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARVPYKVAVIGTGRVGATFAYTMAIVPGVARMVLVDAVPGLSKGVMEDIKHAAAVFRRS
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
IQVEAYDDVSKVENADAIVITAGKPRKADMSRRDLAKVNAQIIRDIGDKLRDRNPGAFYM
CCEEECCCHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
VITNPVDVMTMILSDVIGNKGTVIGTGTSLDTYRFRSAVSELLNEPIAAIDGYVVGEHGE
EECCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCHHHHCCEEEECCCC
EAFVAWSTVTVKGVPIDEYIKEKGLDLSRSRIEEYVKEVAATIIAKQGATIWGPAATFQE
CEEEEEEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHH
IVVSHLANEGRIIPVSVVQEVPGVGRVAVSVPTKISGRLVPLPQLLNEEERERLKRAAEA
HHHHHHCCCCCEEEHHHHHHCCCCCEEEEECCCCCCCCEECCHHHCCHHHHHHHHHHHHH
IKRVYEEAVSS
HHHHHHHHHCC
>Mature Secondary Structure 
ARVPYKVAVIGTGRVGATFAYTMAIVPGVARMVLVDAVPGLSKGVMEDIKHAAAVFRRS
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
IQVEAYDDVSKVENADAIVITAGKPRKADMSRRDLAKVNAQIIRDIGDKLRDRNPGAFYM
CCEEECCCHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
VITNPVDVMTMILSDVIGNKGTVIGTGTSLDTYRFRSAVSELLNEPIAAIDGYVVGEHGE
EECCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCHHHHCCEEEECCCC
EAFVAWSTVTVKGVPIDEYIKEKGLDLSRSRIEEYVKEVAATIIAKQGATIWGPAATFQE
CEEEEEEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHH
IVVSHLANEGRIIPVSVVQEVPGVGRVAVSVPTKISGRLVPLPQLLNEEERERLKRAAEA
HHHHHHCCCCCEEEHHHHHHCCCCCEEEEECCCCCCCCEECCHHHCCHHHHHHHHHHHHH
IKRVYEEAVSS
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2497031 [H]