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The map label for this gene is metG [H]

Identifier: 156936905

GI number: 156936905

Start: 102360

End: 102695

Strand: Reverse

Name: metG [H]

Synonym: Igni_0110

Alternate gene names: 156936905

Gene position: 102695-102360 (Counterclockwise)

Preceding gene: 156936906

Following gene: 156936904

Centisome position: 7.91

GC content: 55.06

Gene sequence:

>336_bases
GTGGAGACCGTAGACTTTAACACCTTTAGTAAGGTAGACTTAAGGGTCGGCAAGGTGATAGAGGCAGAGGACGTGCCCGA
GTCGAGGAAGCTGATAAAACTAATAGTCGATCTAGGGCCCCTAGGGAAGAAACAGATACTCAGCGGGATAAAGAAGTGGT
ACAGACCCGAGGAGCTCGTAGGCCGTTACGTCGTGGTCGTCGCTAACTTGGAGCCCAAGAAGATGGCCGGTCTGGTGTCT
GAAGGGATGGTACTGATGGCCCAAGAGGACGAGGGGAGCAAGCCCGTGTTCGTGGTCCCCCAAGAGCCCGTAAGGCCCGG
AACGAAGGTGTTCTGA

Upstream 100 bases:

>100_bases
CGAAGCGTTAGAAAGGGTAGAGGAGGTCCTCCGTTACTTCGAAGAAAACGTTAGTTGGTTCTAAAGCCCGCGGGTCGAGA
AGCCCCTAGGGTGTGACGTC

Downstream 100 bases:

>100_bases
GCATGTACGTTTGGTTCCCTTCGAACCCTAAGTACATAAAGTTTATGTACGGGGCACCGGCACTCTTGATCTCATGGGCC
GTGTTGACTATAGCTTTCGG

Product: tRNA-binding domain-containing protein

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 111; Mature: 111

Protein sequence:

>111_residues
METVDFNTFSKVDLRVGKVIEAEDVPESRKLIKLIVDLGPLGKKQILSGIKKWYRPEELVGRYVVVVANLEPKKMAGLVS
EGMVLMAQEDEGSKPVFVVPQEPVRPGTKVF

Sequences:

>Translated_111_residues
METVDFNTFSKVDLRVGKVIEAEDVPESRKLIKLIVDLGPLGKKQILSGIKKWYRPEELVGRYVVVVANLEPKKMAGLVS
EGMVLMAQEDEGSKPVFVVPQEPVRPGTKVF
>Mature_111_residues
METVDFNTFSKVDLRVGKVIEAEDVPESRKLIKLIVDLGPLGKKQILSGIKKWYRPEELVGRYVVVVANLEPKKMAGLVS
EGMVLMAQEDEGSKPVFVVPQEPVRPGTKVF

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0073

COG function: function code R; EMAP domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI4507947, Length=123, Percent_Identity=35.7723577235772, Blast_Score=62, Evalue=8e-11,
Organism=Escherichia coli, GI1788432, Length=111, Percent_Identity=44.1441441441441, Blast_Score=84, Evalue=2e-18,
Organism=Escherichia coli, GI1789455, Length=110, Percent_Identity=37.2727272727273, Blast_Score=78, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21357223, Length=84, Percent_Identity=40.4761904761905, Blast_Score=65, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24651924, Length=94, Percent_Identity=38.2978723404255, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 12367; Mature: 12367

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METVDFNTFSKVDLRVGKVIEAEDVPESRKLIKLIVDLGPLGKKQILSGIKKWYRPEELV
CCCCCCCCCCCEEEEECCEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHC
GRYVVVVANLEPKKMAGLVSEGMVLMAQEDEGSKPVFVVPQEPVRPGTKVF
CEEEEEEECCCHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure
METVDFNTFSKVDLRVGKVIEAEDVPESRKLIKLIVDLGPLGKKQILSGIKKWYRPEELV
CCCCCCCCCCCEEEEECCEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHC
GRYVVVVANLEPKKMAGLVSEGMVLMAQEDEGSKPVFVVPQEPVRPGTKVF
CEEEEEEECCCHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]