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The map label for this gene is thiC

Identifier: 156936899

GI number: 156936899

Start: 96780

End: 98099

Strand: Direct

Name: thiC

Synonym: Igni_0104

Alternate gene names: 156936899

Gene position: 96780-98099 (Clockwise)

Preceding gene: 156936898

Following gene: 156936917

Centisome position: 7.46

GC content: 57.73

Gene sequence:

>1320_bases
ATGACTATCTACAAAGACGCACGCAACGGGAGGATCACAGAAGAGATGAAGAAGATTGCCGAAGTGGAGGGGGTAGACCC
GGAGTTCGTGAGGAGGGGTCTAGCGGCGGGGCGAATAGTGCTCCTTAGGAACCTCAAGAGGGCTGACAGAGTAAAGACCG
TGGCCGTTGGGGAGGGAATGTTAACCAAAGTCAACGCTAACATAGGCACGAGCAACACTCTAATAGACGTAAATATGGAG
GTCGAGAAGGCGAAGATAGCTAAGAAGTACGGAGCGGACACAGTGATGGACCTGTCCACCGGCGGGAACTTGGACGTCAT
AAGGAGGAAGATCATGGAAGCGGCCGAACCCCTCCCCCTGGGCACAGTACCCATATACCAAGCCTTCATGGACGTGGCTA
CTCGCAAGGGAGCGGGACTCTACATGACTGAGGACGACATACTGAACACTATAGAGAAGCACCTTAAGGACGGAGTAGAC
TTCATGACCCTTCACGCCGCCTTGACGAGGGATCTGGCGGCGAAGGCCGTTAAGAGCGACCGAGCGGAGCCCATAGTGAG
CAGAGGGGGCTCGATACTAGCTGCTTGGATGCTCGAACACGGGAAGGAGAACCCGCTCCTCTCTAACTTCGACTACATCC
TAGAGATGTTTAAGGAGTACGACGCGGTCATCAGCTTGGGCGACAGCCTGAGGCCCGGCGCCCTCGAGGACGCCCACGAC
GAGTACCACTTGAGCGAGCTCATGGTAAACGCGAGGCTGGTGAAGAGGGCTCGAGAGGCCGGGGTACAAGTTATGCTGGA
GGGGCCGGGCCACGTGCCCTTGGACAGGGTGGTGGCTGACGTAAAGCTCGCGAAGAGGCTTACTGAGGGCGCGCCCTATT
ACATCCTTGGCCCCCTCGTAACCGACATAGCTGCCGGCTACGACCACATAGCGGGCGCGATCGGAGGGGCCATAGCTGCG
GCCCACGGAGCGGACTTCTTGTGTTACGTGACCCCCGCGGAGCACTTGAACTTGCCCAACCCAGAACAAGTTAGGGAAGG
CGTGATAGCCTTCAGAATAGCCGCCCACGCGGCCGACATAGTGAAGTACCCGGACAGGGCGATGAAGGTTGACATAGAGA
TGGGGAGGTGCAGAGGGAGGCTGGACTGGGAGTGTATGATAAGGCTCTCCCTCGACCCGGACAAGGCGAGGGAGATAAGG
AACCAGTACGGCCCTACCTCCATAAAGAGCTGCAACATGTGCGGAAGCTTATGCGTCTTCCTGCTGTTGGACAAGTGGAG
GAGGAAGAAGGACGAAGAGCTTCACGCCCCCCTCGCGTGA

Upstream 100 bases:

>100_bases
GTCAGGATACTCGACTGAACGCGTTTTTTGTTTACCGCTTGCATAGCCCTACCCTAATATTTAATACCCAATGGTCTGTG
TGGACTAAAGGTCCCACGCC

Downstream 100 bases:

>100_bases
AGGTTTTCCACCATCTCGCTTAACCTCCTTAAGGCTCGGAGCCTCTCCTTCTCCCCCACCTTAGCCCTCGCCACTACCTC
TTCCAATATCTTGATCACCT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MTIYKDARNGRITEEMKKIAEVEGVDPEFVRRGLAAGRIVLLRNLKRADRVKTVAVGEGMLTKVNANIGTSNTLIDVNME
VEKAKIAKKYGADTVMDLSTGGNLDVIRRKIMEAAEPLPLGTVPIYQAFMDVATRKGAGLYMTEDDILNTIEKHLKDGVD
FMTLHAALTRDLAAKAVKSDRAEPIVSRGGSILAAWMLEHGKENPLLSNFDYILEMFKEYDAVISLGDSLRPGALEDAHD
EYHLSELMVNARLVKRAREAGVQVMLEGPGHVPLDRVVADVKLAKRLTEGAPYYILGPLVTDIAAGYDHIAGAIGGAIAA
AHGADFLCYVTPAEHLNLPNPEQVREGVIAFRIAAHAADIVKYPDRAMKVDIEMGRCRGRLDWECMIRLSLDPDKAREIR
NQYGPTSIKSCNMCGSLCVFLLLDKWRRKKDEELHAPLA

Sequences:

>Translated_439_residues
MTIYKDARNGRITEEMKKIAEVEGVDPEFVRRGLAAGRIVLLRNLKRADRVKTVAVGEGMLTKVNANIGTSNTLIDVNME
VEKAKIAKKYGADTVMDLSTGGNLDVIRRKIMEAAEPLPLGTVPIYQAFMDVATRKGAGLYMTEDDILNTIEKHLKDGVD
FMTLHAALTRDLAAKAVKSDRAEPIVSRGGSILAAWMLEHGKENPLLSNFDYILEMFKEYDAVISLGDSLRPGALEDAHD
EYHLSELMVNARLVKRAREAGVQVMLEGPGHVPLDRVVADVKLAKRLTEGAPYYILGPLVTDIAAGYDHIAGAIGGAIAA
AHGADFLCYVTPAEHLNLPNPEQVREGVIAFRIAAHAADIVKYPDRAMKVDIEMGRCRGRLDWECMIRLSLDPDKAREIR
NQYGPTSIKSCNMCGSLCVFLLLDKWRRKKDEELHAPLA
>Mature_438_residues
TIYKDARNGRITEEMKKIAEVEGVDPEFVRRGLAAGRIVLLRNLKRADRVKTVAVGEGMLTKVNANIGTSNTLIDVNMEV
EKAKIAKKYGADTVMDLSTGGNLDVIRRKIMEAAEPLPLGTVPIYQAFMDVATRKGAGLYMTEDDILNTIEKHLKDGVDF
MTLHAALTRDLAAKAVKSDRAEPIVSRGGSILAAWMLEHGKENPLLSNFDYILEMFKEYDAVISLGDSLRPGALEDAHDE
YHLSELMVNARLVKRAREAGVQVMLEGPGHVPLDRVVADVKLAKRLTEGAPYYILGPLVTDIAAGYDHIAGAIGGAIAAA
HGADFLCYVTPAEHLNLPNPEQVREGVIAFRIAAHAADIVKYPDRAMKVDIEMGRCRGRLDWECMIRLSLDPDKAREIRN
QYGPTSIKSCNMCGSLCVFLLLDKWRRKKDEELHAPLA

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=397, Percent_Identity=42.3173803526448, Blast_Score=300, Evalue=1e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_IGNH4 (A8A8N6)

Other databases:

- EMBL:   CP000816
- RefSeq:   YP_001434695.1
- STRING:   A8A8N6
- GeneID:   5563131
- GenomeReviews:   CP000816_GR
- KEGG:   iho:Igni_0104
- eggNOG:   arNOG04546
- HOGENOM:   HBG285234
- OMA:   VDFMTIH
- ProtClustDB:   PRK13352
- BioCyc:   IHOS453591:IGNI_0104-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 48371; Mature: 48239

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: BINDING 67-67 BINDING 96-96 BINDING 126-126 BINDING 165-165 BINDING 267-267 BINDING 294-294

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIYKDARNGRITEEMKKIAEVEGVDPEFVRRGLAAGRIVLLRNLKRADRVKTVAVGEGM
CCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHEEEEECCCE
LTKVNANIGTSNTLIDVNMEVEKAKIAKKYGADTVMDLSTGGNLDVIRRKIMEAAEPLPL
EEEEECCCCCCCEEEEEECCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCC
GTVPIYQAFMDVATRKGAGLYMTEDDILNTIEKHLKDGVDFMTLHAALTRDLAAKAVKSD
CCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
RAEPIVSRGGSILAAWMLEHGKENPLLSNFDYILEMFKEYDAVISLGDSLRPGALEDAHD
CCCHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
EYHLSELMVNARLVKRAREAGVQVMLEGPGHVPLDRVVADVKLAKRLTEGAPYYILGPLV
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHH
TDIAAGYDHIAGAIGGAIAAAHGADFLCYVTPAEHLNLPNPEQVREGVIAFRIAAHAADI
HHHHHCHHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHH
VKYPDRAMKVDIEMGRCRGRLDWECMIRLSLDPDKAREIRNQYGPTSIKSCNMCGSLCVF
HHCCCCCEEEEEEHHCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
LLLDKWRRKKDEELHAPLA
HHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TIYKDARNGRITEEMKKIAEVEGVDPEFVRRGLAAGRIVLLRNLKRADRVKTVAVGEGM
CCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHEEEEECCCE
LTKVNANIGTSNTLIDVNMEVEKAKIAKKYGADTVMDLSTGGNLDVIRRKIMEAAEPLPL
EEEEECCCCCCCEEEEEECCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCC
GTVPIYQAFMDVATRKGAGLYMTEDDILNTIEKHLKDGVDFMTLHAALTRDLAAKAVKSD
CCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
RAEPIVSRGGSILAAWMLEHGKENPLLSNFDYILEMFKEYDAVISLGDSLRPGALEDAHD
CCCHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
EYHLSELMVNARLVKRAREAGVQVMLEGPGHVPLDRVVADVKLAKRLTEGAPYYILGPLV
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHH
TDIAAGYDHIAGAIGGAIAAAHGADFLCYVTPAEHLNLPNPEQVREGVIAFRIAAHAADI
HHHHHCHHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHH
VKYPDRAMKVDIEMGRCRGRLDWECMIRLSLDPDKAREIRNQYGPTSIKSCNMCGSLCVF
HHCCCCCEEEEEEHHCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
LLLDKWRRKKDEELHAPLA
HHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA