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Identifier: 15678734

GI number: 15678734

Start: 629245

End: 631104

Strand: Direct

Name: Not Available

Synonym: MTH707

Alternate gene names: 15678734

Gene position: 629245-631104 (Clockwise)

Preceding gene: 15678733

Following gene: 15678735

Centisome position: 35.93

GC content: 54.95

Gene sequence:

>1860_bases
ATGGACTGGGAAAAAGTAGGACTCAAAATGGGCCTTGAAATACACCAGCAGCTTGATACAGAGAGCAAACTCTTCTGTCC
CTGCAGAACAGAGCTCACTGACTCTGAACCCGACCATGATATTGTGAGGAACCTCAGGCCAACCCAGAGCGAGCTCGGAA
AATTCGACAGAGCAGCCTTCGAGGAGGCCATGAGGAAGCTCCACTTCCACTACGAGAACTACCACGAGGAAACATGCCTC
GTTGAGGCTGATGAGGAGCCGCCGCACCCCCTGAACCCTGAGGCACTTGAAATCGCAGTTACAATCGCACTCCTCCTCAA
TATGAGGGTTGTTGATGAGTTCCACACCATGAGGAAGCAGGTGATCGATGGAAGTAACACCGGAGGATTCCAGAGGACAG
GTCTTGTTGCAACCGACGGACACCTCGAAACCCCCCAGGGAACAGTTAAAATAGAGAACCTCTGCCTTGAGGAGGACGCC
GCCAGGAGGATCAGGGAGACAGGGGACGGCGTGGTCTTCAGACTGGACCGCCTGGGCATACCCCTCGTTGAGATAACCAC
CGACCCATCAATGAGCGACCCACAGCAGCTGAGGGAGGTTGCCTACCAGATAGGGCAGATCCTGAGGAGCACCAGGGTCA
AGAGGGGCCTTGGAACCATAAGGCAGGACCTCAACATATCCATACGTGATGGTGCCCGGGTGGAGGTTAAGGGGGTCCAG
GACCTTGACCTCATACCCGAGATAGTTGAGAGGGAGGTTAAGAGGCAGCTCAGTCTGGTTGAGATAAGGGACACCCTCCA
GGAGAGGGGTGCTGTGGTTGAGGATAAGATATTCGATGTGTCAGAGGTCTTCGCAGATACAGAATCCAGGATAATCTCCT
CGGCGGAGTCTGTACTCGCAGTTAAACTGAGGGGCTTTGATGGTCTCATAGGGGTTGAAATCCAGCCAGGAAGGCGTCTC
GGCACCGAGATGGCTGATTATGCTAAAAAGCGGGGTGTGAGTGGCATATTCCACACCGATGAACTCCCTGCCTACGGTAT
AACAGAGGAAGAGGTTCGGGGCCTCAGGGATGCCGTGGGTGCCTCCCAGGGTGATGCGGTTGTCATGGTTGCCCATGAAA
GGGTCACCGCCGAGAATGCCCTCAGGGAGGTTATAAGGCGTGCGGAGATGGCAATCCAGGGTGTGCCTGAGGAGACAAGG
AAGGCGCTGCCTGACGGTAACACACAGTACCTCAGGCCCCTGCCAACCTCAAGCAGGATGTACCTTGAAACAGACATACC
CTTATTCCGGATAGAGGATGACCTCCTGGAGGGCATCAGGAGGAACCTGCCTGAACTCCCCTCAGAGAAGAAGGAGAGGA
TAATGAGGGACTACGGTCTGAGTGAGGACCTGGCATCACAGCTCGTTAAGCGTAACCTTGTTGATGAATTTGAGGCCCTT
ACAGAATTCAGGGTCGATACCACTGTCATAGCATCACTCCTGGCCTACACCCTGAGGGAGCTCAGGAGGGAGGGCCATGA
TGTGGACGGGCTGGGCCTTGATGAACTGAGAGACGCCATTAAACTCCTTGAGGTCGGTAAGATCTCAAAGGATGCCCTCA
GGGACATAGTGGCCTGCATGGCTGATGAGGGCCTTGCAGCGGAGGATGCTGCTCGTAAGCTCAACCTCCTGCTCCTCGCT
GAGGATGAGATCGAGTCCATAATCCAGGAGATCGTTGAGGGTAACCTTGATATGATCTCTGAGAGGGGTATGGGTGCCAT
GGGTCCTCTGATGGGGCAGGCCATGGGCAGGCTCCGTGGAAGGGCAGATGGTAAGGTTGTAAACAGGATCCTCCGGGAGA
AGATCCAGGAGAGGCTCTGA

Upstream 100 bases:

>100_bases
CAGACAGATGACCCTGAAATGGCCCGTGAGATGATGAGGGAGAACATTGCAGGTGAGATCAATGAGAGGACATCGATAGC
ATACTTCAGGGGATGATACA

Downstream 100 bases:

>100_bases
AAAAACCAGCCCCCTTTTTTTATTATTGAGGGTGCTCAGCAGACCCTAAAGCTCCACCTCCTTTTTAATATGAACCGGTT
AACTGAATGTGAAATCTTTA

Product: glutamyl-tRNA(Gln) amidotransferase subunit E

Products: NA

Alternate protein names: Glu-ADT subunit E

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MDWEKVGLKMGLEIHQQLDTESKLFCPCRTELTDSEPDHDIVRNLRPTQSELGKFDRAAFEEAMRKLHFHYENYHEETCL
VEADEEPPHPLNPEALEIAVTIALLLNMRVVDEFHTMRKQVIDGSNTGGFQRTGLVATDGHLETPQGTVKIENLCLEEDA
ARRIRETGDGVVFRLDRLGIPLVEITTDPSMSDPQQLREVAYQIGQILRSTRVKRGLGTIRQDLNISIRDGARVEVKGVQ
DLDLIPEIVEREVKRQLSLVEIRDTLQERGAVVEDKIFDVSEVFADTESRIISSAESVLAVKLRGFDGLIGVEIQPGRRL
GTEMADYAKKRGVSGIFHTDELPAYGITEEEVRGLRDAVGASQGDAVVMVAHERVTAENALREVIRRAEMAIQGVPEETR
KALPDGNTQYLRPLPTSSRMYLETDIPLFRIEDDLLEGIRRNLPELPSEKKERIMRDYGLSEDLASQLVKRNLVDEFEAL
TEFRVDTTVIASLLAYTLRELRREGHDVDGLGLDELRDAIKLLEVGKISKDALRDIVACMADEGLAAEDAARKLNLLLLA
EDEIESIIQEIVEGNLDMISERGMGAMGPLMGQAMGRLRGRADGKVVNRILREKIQERL

Sequences:

>Translated_619_residues
MDWEKVGLKMGLEIHQQLDTESKLFCPCRTELTDSEPDHDIVRNLRPTQSELGKFDRAAFEEAMRKLHFHYENYHEETCL
VEADEEPPHPLNPEALEIAVTIALLLNMRVVDEFHTMRKQVIDGSNTGGFQRTGLVATDGHLETPQGTVKIENLCLEEDA
ARRIRETGDGVVFRLDRLGIPLVEITTDPSMSDPQQLREVAYQIGQILRSTRVKRGLGTIRQDLNISIRDGARVEVKGVQ
DLDLIPEIVEREVKRQLSLVEIRDTLQERGAVVEDKIFDVSEVFADTESRIISSAESVLAVKLRGFDGLIGVEIQPGRRL
GTEMADYAKKRGVSGIFHTDELPAYGITEEEVRGLRDAVGASQGDAVVMVAHERVTAENALREVIRRAEMAIQGVPEETR
KALPDGNTQYLRPLPTSSRMYLETDIPLFRIEDDLLEGIRRNLPELPSEKKERIMRDYGLSEDLASQLVKRNLVDEFEAL
TEFRVDTTVIASLLAYTLRELRREGHDVDGLGLDELRDAIKLLEVGKISKDALRDIVACMADEGLAAEDAARKLNLLLLA
EDEIESIIQEIVEGNLDMISERGMGAMGPLMGQAMGRLRGRADGKVVNRILREKIQERL
>Mature_619_residues
MDWEKVGLKMGLEIHQQLDTESKLFCPCRTELTDSEPDHDIVRNLRPTQSELGKFDRAAFEEAMRKLHFHYENYHEETCL
VEADEEPPHPLNPEALEIAVTIALLLNMRVVDEFHTMRKQVIDGSNTGGFQRTGLVATDGHLETPQGTVKIENLCLEEDA
ARRIRETGDGVVFRLDRLGIPLVEITTDPSMSDPQQLREVAYQIGQILRSTRVKRGLGTIRQDLNISIRDGARVEVKGVQ
DLDLIPEIVEREVKRQLSLVEIRDTLQERGAVVEDKIFDVSEVFADTESRIISSAESVLAVKLRGFDGLIGVEIQPGRRL
GTEMADYAKKRGVSGIFHTDELPAYGITEEEVRGLRDAVGASQGDAVVMVAHERVTAENALREVIRRAEMAIQGVPEETR
KALPDGNTQYLRPLPTSSRMYLETDIPLFRIEDDLLEGIRRNLPELPSEKKERIMRDYGLSEDLASQLVKRNLVDEFEAL
TEFRVDTTVIASLLAYTLRELRREGHDVDGLGLDELRDAIKLLEVGKISKDALRDIVACMADEGLAAEDAARKLNLLLLA
EDEIESIIQEIVEGNLDMISERGMGAMGPLMGQAMGRLRGRADGKVVNRILREKIQERL

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG2511

COG function: function code J; Archaeal Glu-tRNAGln amidotransferase subunit E (contains GAD domain)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatE subfamily

Homologues:

Organism=Saccharomyces cerevisiae, GI6319391, Length=280, Percent_Identity=26.7857142857143, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATE_METTH (O26803)

Other databases:

- EMBL:   AE000666
- PIR:   D69194
- RefSeq:   NP_275850.1
- PDB:   2D6F
- PDBsum:   2D6F
- ProteinModelPortal:   O26803
- STRING:   O26803
- GeneID:   1470668
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH707
- NMPDR:   fig|187420.1.peg.705
- eggNOG:   arNOG04528
- HOGENOM:   HBG297405
- OMA:   TSGFQRT
- ProtClustDB:   PRK04028
- BioCyc:   MetaCyc:MONOMER-14999
- BioCyc:   MTHE187420:MTH707-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00588
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004115
- InterPro:   IPR023168
- InterPro:   IPR004414
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00134

Pfam domain/function: PF02938 GAD; PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 69534; Mature: 69534

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDWEKVGLKMGLEIHQQLDTESKLFCPCRTELTDSEPDHDIVRNLRPTQSELGKFDRAAF
CCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHH
EEAMRKLHFHYENYHEETCLVEADEEPPHPLNPEALEIAVTIALLLNMRVVDEFHTMRKQ
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIDGSNTGGFQRTGLVATDGHLETPQGTVKIENLCLEEDAARRIRETGDGVVFRLDRLGI
HHCCCCCCCCEECCEEEECCCCCCCCCCEEHHHHHHCHHHHHHHHHCCCCEEEEECCCCC
PLVEITTDPSMSDPQQLREVAYQIGQILRSTRVKRGLGTIRQDLNISIRDGARVEVKGVQ
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECCCC
DLDLIPEIVEREVKRQLSLVEIRDTLQERGAVVEDKIFDVSEVFADTESRIISSAESVLA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHE
VKLRGFDGLIGVEIQPGRRLGTEMADYAKKRGVSGIFHTDELPAYGITEEEVRGLRDAVG
EEEECCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHHHC
ASQGDAVVMVAHERVTAENALREVIRRAEMAIQGVPEETRKALPDGNTQYLRPLPTSSRM
CCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCEECCCCCCCCE
YLETDIPLFRIEDDLLEGIRRNLPELPSEKKERIMRDYGLSEDLASQLVKRNLVDEFEAL
EEEECCCEEEECHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
TEFRVDTTVIASLLAYTLRELRREGHDVDGLGLDELRDAIKLLEVGKISKDALRDIVACM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ADEGLAAEDAARKLNLLLLAEDEIESIIQEIVEGNLDMISERGMGAMGPLMGQAMGRLRG
HCCCCCHHHHHHHEEEEEEEHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHCC
RADGKVVNRILREKIQERL
CCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDWEKVGLKMGLEIHQQLDTESKLFCPCRTELTDSEPDHDIVRNLRPTQSELGKFDRAAF
CCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHH
EEAMRKLHFHYENYHEETCLVEADEEPPHPLNPEALEIAVTIALLLNMRVVDEFHTMRKQ
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIDGSNTGGFQRTGLVATDGHLETPQGTVKIENLCLEEDAARRIRETGDGVVFRLDRLGI
HHCCCCCCCCEECCEEEECCCCCCCCCCEEHHHHHHCHHHHHHHHHCCCCEEEEECCCCC
PLVEITTDPSMSDPQQLREVAYQIGQILRSTRVKRGLGTIRQDLNISIRDGARVEVKGVQ
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECCCC
DLDLIPEIVEREVKRQLSLVEIRDTLQERGAVVEDKIFDVSEVFADTESRIISSAESVLA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHE
VKLRGFDGLIGVEIQPGRRLGTEMADYAKKRGVSGIFHTDELPAYGITEEEVRGLRDAVG
EEEECCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHHHC
ASQGDAVVMVAHERVTAENALREVIRRAEMAIQGVPEETRKALPDGNTQYLRPLPTSSRM
CCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCEECCCCCCCCE
YLETDIPLFRIEDDLLEGIRRNLPELPSEKKERIMRDYGLSEDLASQLVKRNLVDEFEAL
EEEECCCEEEECHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
TEFRVDTTVIASLLAYTLRELRREGHDVDGLGLDELRDAIKLLEVGKISKDALRDIVACM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ADEGLAAEDAARKLNLLLLAEDEIESIIQEIVEGNLDMISERGMGAMGPLMGQAMGRLRG
HCCCCCHHHHHHHEEEEEEEHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHCC
RADGKVVNRILREKIQERL
CCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463; 10993083