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Identifier: 15678733

GI number: 15678733

Start: 627933

End: 629240

Strand: Direct

Name: Not Available

Synonym: MTH706

Alternate gene names: 15678733

Gene position: 627933-629240 (Clockwise)

Preceding gene: 15678732

Following gene: 15678734

Centisome position: 35.85

GC content: 53.52

Gene sequence:

>1308_bases
ATGTCCTACCAGGGAAGAGCAAGGAAATTCCTGGAATCAGCCTCCATAGATGTGGGGGACATGGTGCTGGTTGAGAAACC
AGACGTCACCTATGAGGGCATGGTCCTTGACCGTGCAGACGATGCAGATGACAGACACATAGTCCTGAAACTTGAAAACG
GTTACAATATCGGAGTTGAAATCAGCGATGCGCGGATAGAGCTCCTTGAGAAGGGATCCGAGCCAAGGATAGAACTCCCG
CCAGTTGAAGCCGCTGAAGATCCAGAATTACCTGATGTATCAATAATATCAACAGGTGGTACCGTTGCATCCATAATAGA
TTACCGCACAGGGGCTGTTCACCCTGCATTCACAGCAGATGACCTTCTCCGTGCAAACCCCGAACTCCTGGACATTGCAA
ATATAAGGGGGCGTGCAGTTTTCAACATACTCAGCGAGAACATGAAACCAGAATACTGGGTCGAAACAGCCAGGGCAGTT
TACGGTGAGATAAAGGATGGTGCAGATGGGGTGGTGGTAGCCCATGGAACAGACACCATGCACTACACCTCAGCCGCCCT
CAGTTTCATGCTCAGGACCCCGGTACCTGTGGTATTCACCGGCGCCCAGAGGAGCTCAGACAGACCCTCCTCAGATGCAA
GCCTCAACATACAGTGCTCTGTCAGGGCAGCGACCTCAGAAATCGCAGAGGTCACAGTCTGCATGCACGCGACCATGGAC
GACCTCAGCTGCCACCTCCACCGGGGCGTGAAGGTGAGGAAGATGCACACATCCCGGAGGGACACCTTCAGAAGCATGAA
CGCCCTTCCCCTTGCAGAGGTGACACCCGATGGCATTAAAATCCTCGAGGAAAACTACAGAAAAAGGGGCTCAGATGAAC
TGGAACTCAGCGACCGCGTGGAGGAGAGGGTCGCCTTCATAAAGAGCTACCCTGGAATATCCCCTGATATCATAAAATGG
CACCTCGATGAGGGCTACAGGGGTATCGTCATTGAGGGCACAGGCCTCGGTCACTGCCCTGACACCCTGATACCGGTCAT
AGGGGAGGCCCATGATATGGGAGTCCCTGTGGCAATGACGTCCCAGTGCCTGAACGGAAGGGTGAACATGAACGTCTACA
GTACAGGGCGAAGACTGCTGCAGGCAGGTGTCATACCCTGCGATGACATGCTCCCGGAGGTTGCCTACGTGAAGATGTGC
TGGGTCCTGGGACAGACAGATGACCCTGAAATGGCCCGTGAGATGATGAGGGAGAACATTGCAGGTGAGATCAATGAGAG
GACATCGATAGCATACTTCAGGGGATGA

Upstream 100 bases:

>100_bases
AATTCACCTTCGCAGGTAAGGAAAAGATCATAGATATGAACCTGAGGATCCTTGAGGCAGGTGCAGAGTGGGCAAGAGAA
AACATTGAGGGGGAATTATA

Downstream 100 bases:

>100_bases
TACAATGGACTGGGAAAAAGTAGGACTCAAAATGGGCCTTGAAATACACCAGCAGCTTGATACAGAGAGCAAACTCTTCT
GTCCCTGCAGAACAGAGCTC

Product: glutamyl-tRNA(Gln) amidotransferase subunit D

Products: L-aspartate; NH3

Alternate protein names: Glu-ADT subunit D

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSYQGRARKFLESASIDVGDMVLVEKPDVTYEGMVLDRADDADDRHIVLKLENGYNIGVEISDARIELLEKGSEPRIELP
PVEAAEDPELPDVSIISTGGTVASIIDYRTGAVHPAFTADDLLRANPELLDIANIRGRAVFNILSENMKPEYWVETARAV
YGEIKDGADGVVVAHGTDTMHYTSAALSFMLRTPVPVVFTGAQRSSDRPSSDASLNIQCSVRAATSEIAEVTVCMHATMD
DLSCHLHRGVKVRKMHTSRRDTFRSMNALPLAEVTPDGIKILEENYRKRGSDELELSDRVEERVAFIKSYPGISPDIIKW
HLDEGYRGIVIEGTGLGHCPDTLIPVIGEAHDMGVPVAMTSQCLNGRVNMNVYSTGRRLLQAGVIPCDDMLPEVAYVKMC
WVLGQTDDPEMAREMMRENIAGEINERTSIAYFRG

Sequences:

>Translated_435_residues
MSYQGRARKFLESASIDVGDMVLVEKPDVTYEGMVLDRADDADDRHIVLKLENGYNIGVEISDARIELLEKGSEPRIELP
PVEAAEDPELPDVSIISTGGTVASIIDYRTGAVHPAFTADDLLRANPELLDIANIRGRAVFNILSENMKPEYWVETARAV
YGEIKDGADGVVVAHGTDTMHYTSAALSFMLRTPVPVVFTGAQRSSDRPSSDASLNIQCSVRAATSEIAEVTVCMHATMD
DLSCHLHRGVKVRKMHTSRRDTFRSMNALPLAEVTPDGIKILEENYRKRGSDELELSDRVEERVAFIKSYPGISPDIIKW
HLDEGYRGIVIEGTGLGHCPDTLIPVIGEAHDMGVPVAMTSQCLNGRVNMNVYSTGRRLLQAGVIPCDDMLPEVAYVKMC
WVLGQTDDPEMAREMMRENIAGEINERTSIAYFRG
>Mature_434_residues
SYQGRARKFLESASIDVGDMVLVEKPDVTYEGMVLDRADDADDRHIVLKLENGYNIGVEISDARIELLEKGSEPRIELPP
VEAAEDPELPDVSIISTGGTVASIIDYRTGAVHPAFTADDLLRANPELLDIANIRGRAVFNILSENMKPEYWVETARAVY
GEIKDGADGVVVAHGTDTMHYTSAALSFMLRTPVPVVFTGAQRSSDRPSSDASLNIQCSVRAATSEIAEVTVCMHATMDD
LSCHLHRGVKVRKMHTSRRDTFRSMNALPLAEVTPDGIKILEENYRKRGSDELELSDRVEERVAFIKSYPGISPDIIKWH
LDEGYRGIVIEGTGLGHCPDTLIPVIGEAHDMGVPVAMTSQCLNGRVNMNVYSTGRRLLQAGVIPCDDMLPEVAYVKMCW
VLGQTDDPEMAREMMRENIAGEINERTSIAYFRG

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family. GatD subfamily

Homologues:

Organism=Homo sapiens, GI122937331, Length=286, Percent_Identity=31.1188811188811, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1788065, Length=286, Percent_Identity=34.6153846153846, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1789327, Length=336, Percent_Identity=29.1666666666667, Blast_Score=116, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI193207372, Length=307, Percent_Identity=31.5960912052117, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17558280, Length=307, Percent_Identity=31.5960912052117, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17558278, Length=307, Percent_Identity=31.5960912052117, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI193207374, Length=306, Percent_Identity=31.0457516339869, Blast_Score=124, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6323189, Length=292, Percent_Identity=27.3972602739726, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323187, Length=292, Percent_Identity=27.3972602739726, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323186, Length=292, Percent_Identity=27.3972602739726, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323184, Length=292, Percent_Identity=27.3972602739726, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320527, Length=305, Percent_Identity=24.5901639344262, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24639609, Length=285, Percent_Identity=30.8771929824561, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24645530, Length=278, Percent_Identity=30.9352517985612, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATD_METTH (O26802)

Other databases:

- EMBL:   AE000666
- PIR:   C69194
- RefSeq:   NP_275849.1
- PDB:   2D6F
- PDBsum:   2D6F
- ProteinModelPortal:   O26802
- SMR:   O26802
- STRING:   O26802
- GeneID:   1470667
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH706
- NMPDR:   fig|187420.1.peg.704
- eggNOG:   arNOG04527
- HOGENOM:   HBG497495
- OMA:   KMKSGYN
- PhylomeDB:   O26802
- ProtClustDB:   PRK04183
- BioCyc:   MetaCyc:MONOMER-14998
- BioCyc:   MTHE187420:MTH706-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00586
- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827
- InterPro:   IPR011878
- PANTHER:   PTHR11707
- PRINTS:   PR00139
- SMART:   SM00870
- TIGRFAMs:   TIGR00519
- TIGRFAMs:   TIGR02153

Pfam domain/function: PF00710 Asparaginase; SSF53774 Asp/Glutamnse

EC number: 3.5.1.1

Molecular weight: Translated: 47998; Mature: 47867

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00144 ASN_GLN_ASE_1; PS00917 ASN_GLN_ASE_2

Important sites: ACT_SITE 101-101 ACT_SITE 177-177 ACT_SITE 178-178 ACT_SITE 254-254

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYQGRARKFLESASIDVGDMVLVEKPDVTYEGMVLDRADDADDRHIVLKLENGYNIGVE
CCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCEEEEEECCCCEEEEE
ISDARIELLEKGSEPRIELPPVEAAEDPELPDVSIISTGGTVASIIDYRTGAVHPAFTAD
ECHHHHHHHHCCCCCCEECCCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCCCCCCCCHH
DLLRANPELLDIANIRGRAVFNILSENMKPEYWVETARAVYGEIKDGADGVVVAHGTDTM
HHHHCCCCEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCH
HYTSAALSFMLRTPVPVVFTGAQRSSDRPSSDASLNIQCSVRAATSEIAEVTVCMHATMD
HHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCEEEEEEEEEHHHHHHHHEEHHHHCCHH
DLSCHLHRGVKVRKMHTSRRDTFRSMNALPLAEVTPDGIKILEENYRKRGSDELELSDRV
HHHHHHHCCEEEEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHH
EERVAFIKSYPGISPDIIKWHLDEGYRGIVIEGTGLGHCPDTLIPVIGEAHDMGVPVAMT
HHHHHHHHHCCCCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCCHHHH
SQCLNGRVNMNVYSTGRRLLQAGVIPCDDMLPEVAYVKMCWVLGQTDDPEMAREMMRENI
HHHHCCEEEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
AGEINERTSIAYFRG
CCCCCCCEEEEEECC
>Mature Secondary Structure 
SYQGRARKFLESASIDVGDMVLVEKPDVTYEGMVLDRADDADDRHIVLKLENGYNIGVE
CCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCEEEEEECCCCEEEEE
ISDARIELLEKGSEPRIELPPVEAAEDPELPDVSIISTGGTVASIIDYRTGAVHPAFTAD
ECHHHHHHHHCCCCCCEECCCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCCCCCCCCHH
DLLRANPELLDIANIRGRAVFNILSENMKPEYWVETARAVYGEIKDGADGVVVAHGTDTM
HHHHCCCCEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCH
HYTSAALSFMLRTPVPVVFTGAQRSSDRPSSDASLNIQCSVRAATSEIAEVTVCMHATMD
HHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCEEEEEEEEEHHHHHHHHEEHHHHCCHH
DLSCHLHRGVKVRKMHTSRRDTFRSMNALPLAEVTPDGIKILEENYRKRGSDELELSDRV
HHHHHHHCCEEEEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHH
EERVAFIKSYPGISPDIIKWHLDEGYRGIVIEGTGLGHCPDTLIPVIGEAHDMGVPVAMT
HHHHHHHHHCCCCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCCHHHH
SQCLNGRVNMNVYSTGRRLLQAGVIPCDDMLPEVAYVKMCWVLGQTDDPEMAREMMRENI
HHHHCCEEEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
AGEINERTSIAYFRG
CCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-asparagine; H2O

Specific reaction: L-asparagine + H2O = L-aspartate + NH3

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: 5-Bromo-4-oxo-L-norvaline; Hg2+; NH3 [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463; 10993083