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The map label for this gene is gltX

Identifier: 15678080

GI number: 15678080

Start: 29608

End: 31269

Strand: Direct

Name: gltX

Synonym: MTH51

Alternate gene names: 15678080

Gene position: 29608-31269 (Clockwise)

Preceding gene: 15678079

Following gene: 15678081

Centisome position: 1.69

GC content: 57.4

Gene sequence:

>1662_bases
GTGGTGCCAGTGGAGGACCTTGTCTACAGGTACGCACTCCTGAACGCTGTGAAACACAGGGGAAGGGCAAACCCGGGGGC
CGTCATGGGGGCCGTCATGAGCAACGAACCTGAACTCAGGAAGATGGCGCCCCAGGTTAAGGAAGCAGTTGAGGCCGCTG
TTGAGAGGGTTAACAGTTTAAGCCCCGAGGAGCAGCAGCAGGAGATGGAGAGGCTGGGCCTTGAGATCACCGAGAGGAAA
CAGAAGAAGCGGAAGGGTCTAAGGGAACTGGCCGGGGTGAAGGGTGAGGTGGTCCTCAGGTTCGCCCCCAACCCCAGCGG
ACCCCTCCACATAGGCCATGCAAGGGCCGCGATCCTCAACCATGAATATGCAAGGAAATATGACGGCAGGCTCATCCTCA
GGATAGAGGACACGGACCCCCGCAGGGTTGACCCGGAGGCCTACGATATGATTCCAGCCGACCTTGAGTGGCTGGGCGTT
GAATGGGATGAGACAGTTATCCAGAGCGACCGCATGGAAACCTACTATGAGTACACAGAGAAACTCATAGAGAGGGGTGG
TGCGTATGTATGCACATGCAGGCCGGAGGAGTTCAGGGAACTCAAGAACAGGGGAGAGGCCTGTCACTGCAGGTCCCTTG
GTTTCAGGGAGAACCTCCAGCGATGGAGGGAAATGTTCGAGATGAAGGAGGGCTCAGCCGTTGTGAGGGTTAAAACGGAC
CTGAACCACCCAAACCCTGCCATAAGAGACTGGGTCTCAATGAGGATCGTTGAGGCAGAGCACCCACGCACCGGTACACG
CTACAGGGTCTACCCCATGATGAACTTCTCAGTGGCGGTTGATGACCACCTCCTTGGCGTGACACACGTCCTGAGGGGTA
AGGACCACCTGGCAAACAGAGAGAAGCAGGAGTACCTCTACAGGCACCTTGGCTGGGAGCCCCCCGAATTCATACACTAC
GGGCGCCTGAAGATGGACGACGTTGCACTCAGCACCTCGGGGGCCCGTGAGGGCATCCTCAGGGGTGAGTATTCTGGATG
GGACGACCCACGCCTCGGAACCCTGAGGGCCATTGCAAGGAGGGGTATACGACCGGAGGCCATAAGAAAGTTAATGGTTG
AAATCGGCGTAAAGATAGCAGATTCCACAATGAGCTGGAAGAAGATCTACGGCCTCAACAGGAGCATCCTCGAAGAGGAG
GCCAGGAGGTACTTCTTTGCTGCAGATCCTGTTAAACTTGAAGTGGTGGGTTTACCCGGACCTGTCAGGGTTGAAAGGCC
CCTACACCCGGACCACCCTGAAATCGGGAACAGGGTACTTGAACTGAGGGGTGAGGTGTACCTGCCGGGCGATGACCTTG
GGGAAGGACCGCTGCGCCTCATAGACGCGGTTAACGTGATCTACTCTGGGGGTGAACTGAGGTACCACAGTGAGGGCATC
GAGGAGGCCCGTGAACTCGGCGCTTCAATGATACACTGGGTCCCTGCAGAGTCCGCCCTTGAGGCCGAGGTCATCATGCC
GGACGCCTCCAGGGTGAGGGGAGTCATCGAGGCTGACGCATCAGAACTGGAGGTTGATGATGTTGTGCAGCTTGAGAGGT
TCGGCTTCGCCCGCCTGGATTCTGCAGGCCCAGGAATGGTCTTCTACTATGCCCACAAATGA

Upstream 100 bases:

>100_bases
TGTGAGGATGGCAAAGGAACGCCTCGAGGTACTTGAGGAATCGGATGCAAGGGAAGCCCTCGCCATGATAGCCGACTTCG
TCCTTGAAAGGGAACACTAG

Downstream 100 bases:

>100_bases
TTTTTCTGTTTTCTGGGGTTTATAATTCCTTTTTTAACAGGGTATCCCTGAGAAAGAATTTTTTGTTTTCCAGCAGTGAT
CCGGCTTCTTCACAGGCTGG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MVPVEDLVYRYALLNAVKHRGRANPGAVMGAVMSNEPELRKMAPQVKEAVEAAVERVNSLSPEEQQQEMERLGLEITERK
QKKRKGLRELAGVKGEVVLRFAPNPSGPLHIGHARAAILNHEYARKYDGRLILRIEDTDPRRVDPEAYDMIPADLEWLGV
EWDETVIQSDRMETYYEYTEKLIERGGAYVCTCRPEEFRELKNRGEACHCRSLGFRENLQRWREMFEMKEGSAVVRVKTD
LNHPNPAIRDWVSMRIVEAEHPRTGTRYRVYPMMNFSVAVDDHLLGVTHVLRGKDHLANREKQEYLYRHLGWEPPEFIHY
GRLKMDDVALSTSGAREGILRGEYSGWDDPRLGTLRAIARRGIRPEAIRKLMVEIGVKIADSTMSWKKIYGLNRSILEEE
ARRYFFAADPVKLEVVGLPGPVRVERPLHPDHPEIGNRVLELRGEVYLPGDDLGEGPLRLIDAVNVIYSGGELRYHSEGI
EEARELGASMIHWVPAESALEAEVIMPDASRVRGVIEADASELEVDDVVQLERFGFARLDSAGPGMVFYYAHK

Sequences:

>Translated_553_residues
MVPVEDLVYRYALLNAVKHRGRANPGAVMGAVMSNEPELRKMAPQVKEAVEAAVERVNSLSPEEQQQEMERLGLEITERK
QKKRKGLRELAGVKGEVVLRFAPNPSGPLHIGHARAAILNHEYARKYDGRLILRIEDTDPRRVDPEAYDMIPADLEWLGV
EWDETVIQSDRMETYYEYTEKLIERGGAYVCTCRPEEFRELKNRGEACHCRSLGFRENLQRWREMFEMKEGSAVVRVKTD
LNHPNPAIRDWVSMRIVEAEHPRTGTRYRVYPMMNFSVAVDDHLLGVTHVLRGKDHLANREKQEYLYRHLGWEPPEFIHY
GRLKMDDVALSTSGAREGILRGEYSGWDDPRLGTLRAIARRGIRPEAIRKLMVEIGVKIADSTMSWKKIYGLNRSILEEE
ARRYFFAADPVKLEVVGLPGPVRVERPLHPDHPEIGNRVLELRGEVYLPGDDLGEGPLRLIDAVNVIYSGGELRYHSEGI
EEARELGASMIHWVPAESALEAEVIMPDASRVRGVIEADASELEVDDVVQLERFGFARLDSAGPGMVFYYAHK
>Mature_553_residues
MVPVEDLVYRYALLNAVKHRGRANPGAVMGAVMSNEPELRKMAPQVKEAVEAAVERVNSLSPEEQQQEMERLGLEITERK
QKKRKGLRELAGVKGEVVLRFAPNPSGPLHIGHARAAILNHEYARKYDGRLILRIEDTDPRRVDPEAYDMIPADLEWLGV
EWDETVIQSDRMETYYEYTEKLIERGGAYVCTCRPEEFRELKNRGEACHCRSLGFRENLQRWREMFEMKEGSAVVRVKTD
LNHPNPAIRDWVSMRIVEAEHPRTGTRYRVYPMMNFSVAVDDHLLGVTHVLRGKDHLANREKQEYLYRHLGWEPPEFIHY
GRLKMDDVALSTSGAREGILRGEYSGWDDPRLGTLRAIARRGIRPEAIRKLMVEIGVKIADSTMSWKKIYGLNRSILEEE
ARRYFFAADPVKLEVVGLPGPVRVERPLHPDHPEIGNRVLELRGEVYLPGDDLGEGPLRLIDAVNVIYSGGELRYHSEGI
EEARELGASMIHWVPAESALEAEVIMPDASRVRGVIEADASELEVDDVVQLERFGFARLDSAGPGMVFYYAHK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI62241042, Length=383, Percent_Identity=33.6814621409922, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI4826960, Length=389, Percent_Identity=29.305912596401, Blast_Score=159, Evalue=8e-39,
Organism=Homo sapiens, GI134288884, Length=315, Percent_Identity=31.4285714285714, Blast_Score=146, Evalue=6e-35,
Organism=Escherichia coli, GI1786895, Length=509, Percent_Identity=29.8624754420432, Blast_Score=198, Evalue=7e-52,
Organism=Escherichia coli, GI1788743, Length=245, Percent_Identity=30.6122448979592, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI226510925, Length=248, Percent_Identity=32.6612903225806, Blast_Score=108, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17511007, Length=359, Percent_Identity=34.5403899721448, Blast_Score=205, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI17543332, Length=515, Percent_Identity=28.3495145631068, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI71993394, Length=306, Percent_Identity=29.7385620915033, Blast_Score=116, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6324742, Length=375, Percent_Identity=34.4, Blast_Score=224, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6321192, Length=496, Percent_Identity=28.2258064516129, Blast_Score=198, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6324540, Length=339, Percent_Identity=28.6135693215339, Blast_Score=120, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24649466, Length=424, Percent_Identity=33.0188679245283, Blast_Score=206, Evalue=3e-53,
Organism=Drosophila melanogaster, GI17864488, Length=391, Percent_Identity=31.4578005115089, Blast_Score=178, Evalue=7e-45,
Organism=Drosophila melanogaster, GI24665321, Length=262, Percent_Identity=35.1145038167939, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_METTH (O26157)

Other databases:

- EMBL:   AE000666
- PIR:   B69167
- RefSeq:   NP_275194.1
- PDB:   3AII
- PDBsum:   3AII
- ProteinModelPortal:   O26157
- SMR:   O26157
- STRING:   O26157
- GeneID:   1470013
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH51
- NMPDR:   fig|187420.1.peg.49
- eggNOG:   arNOG04636
- HOGENOM:   HBG334108
- OMA:   MRFAPNP
- ProtClustDB:   PRK04156
- BioCyc:   MTHE187420:MTH51-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_A
- InterPro:   IPR001412
- InterPro:   IPR004526
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR022888
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:2.40.240.10
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00463

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C; SSF50715 Ribosomal_L25rel

EC number: =6.1.1.17

Molecular weight: Translated: 63091; Mature: 63091

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPVEDLVYRYALLNAVKHRGRANPGAVMGAVMSNEPELRKMAPQVKEAVEAAVERVNSL
CCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
SPEEQQQEMERLGLEITERKQKKRKGLRELAGVKGEVVLRFAPNPSGPLHIGHARAAILN
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEECHHHHHHHH
HEYARKYDGRLILRIEDTDPRRVDPEAYDMIPADLEWLGVEWDETVIQSDRMETYYEYTE
HHHHHHCCCEEEEEEECCCCCCCCCHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHH
KLIERGGAYVCTCRPEEFRELKNRGEACHCRSLGFRENLQRWREMFEMKEGSAVVRVKTD
HHHHCCCCEEEEECHHHHHHHHCCCCEEEHHCCCHHHHHHHHHHHHHCCCCCEEEEEEEC
LNHPNPAIRDWVSMRIVEAEHPRTGTRYRVYPMMNFSVAVDDHLLGVTHVLRGKDHLANR
CCCCCHHHHHHHHHEEEECCCCCCCCEEEEEEEEEEEEEECCHHHHHHHHHCCCHHHCCH
EKQEYLYRHLGWEPPEFIHYGRLKMDDVALSTSGAREGILRGEYSGWDDPRLGTLRAIAR
HHHHHHHHHCCCCCHHHHHCCCCEECCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
RGIRPEAIRKLMVEIGVKIADSTMSWKKIYGLNRSILEEEARRYFFAADPVKLEVVGLPG
CCCCHHHHHHHHHHHCCEEECCCCCHHHHHCCCHHHHHHHHHHEEEECCCEEEEEEECCC
PVRVERPLHPDHPEIGNRVLELRGEVYLPGDDLGEGPLRLIDAVNVIYSGGELRYHSEGI
CEEECCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHCCCCCEEECHHHH
EEARELGASMIHWVPAESALEAEVIMPDASRVRGVIEADASELEVDDVVQLERFGFARLD
HHHHHHCHHHEEECCCCCCCCCEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCC
SAGPGMVFYYAHK
CCCCCEEEEEECC
>Mature Secondary Structure
MVPVEDLVYRYALLNAVKHRGRANPGAVMGAVMSNEPELRKMAPQVKEAVEAAVERVNSL
CCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
SPEEQQQEMERLGLEITERKQKKRKGLRELAGVKGEVVLRFAPNPSGPLHIGHARAAILN
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEECHHHHHHHH
HEYARKYDGRLILRIEDTDPRRVDPEAYDMIPADLEWLGVEWDETVIQSDRMETYYEYTE
HHHHHHCCCEEEEEEECCCCCCCCCHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHH
KLIERGGAYVCTCRPEEFRELKNRGEACHCRSLGFRENLQRWREMFEMKEGSAVVRVKTD
HHHHCCCCEEEEECHHHHHHHHCCCCEEEHHCCCHHHHHHHHHHHHHCCCCCEEEEEEEC
LNHPNPAIRDWVSMRIVEAEHPRTGTRYRVYPMMNFSVAVDDHLLGVTHVLRGKDHLANR
CCCCCHHHHHHHHHEEEECCCCCCCCEEEEEEEEEEEEEECCHHHHHHHHHCCCHHHCCH
EKQEYLYRHLGWEPPEFIHYGRLKMDDVALSTSGAREGILRGEYSGWDDPRLGTLRAIAR
HHHHHHHHHCCCCCHHHHHCCCCEECCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
RGIRPEAIRKLMVEIGVKIADSTMSWKKIYGLNRSILEEEARRYFFAADPVKLEVVGLPG
CCCCHHHHHHHHHHHCCEEECCCCCHHHHHCCCHHHHHHHHHHEEEECCCEEEEEEECCC
PVRVERPLHPDHPEIGNRVLELRGEVYLPGDDLGEGPLRLIDAVNVIYSGGELRYHSEGI
CEEECCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHCCCCCEEECHHHH
EEARELGASMIHWVPAESALEAEVIMPDASRVRGVIEADASELEVDDVVQLERFGFARLD
HHHHHHCHHHEEECCCCCCCCCEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCC
SAGPGMVFYYAHK
CCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463