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The map label for this gene is ruvA

Identifier: 15674189

GI number: 15674189

Start: 2289550

End: 2290143

Strand: Reverse

Name: ruvA

Synonym: L0266

Alternate gene names: 15674189

Gene position: 2290143-2289550 (Counterclockwise)

Preceding gene: 15674190

Following gene: 15674188

Centisome position: 96.81

GC content: 38.38

Gene sequence:

>594_bases
ATGTTTGAATATCTTAATGGAAAATTAGTAAAAATTTCCCCAACAAATATTGTAATTGATGTAGCAGGAATTGGTTATCT
TATCAGTGTAGCTAACCCTTACGCTTGGTCTGCTTTGATGAACACAGAAGTAAAAATTTATGTTCATCAAGTCATTCGCG
AAGATGCCCACAGCCTCTATGGTTTTGTTAACGAGGCCGAAAAAGCTTTATTCTTACGTCTGATCAGCGTTTCTGGGATT
GGGCCAAAATCAGCTCTGGCCATCATTGCGGCGGCTGATAACGAAGGTTTAATCACTGCTATTGACAATAGTGATATCAA
GTATTTAACTAAATTTCCAGGAGTTGGTAAAAAAACAGCCATGCAGATGGTGCTTGATTTGGCTGGGAAATTTGATGCGA
CAGGAACTGTAGGTATTTCTCTTCTTGATGCTGGACCTGCTGGCAATCTTGCTTTGGAAGAAGCGATTGAAGCGCTGCAA
GCTTTGGGTTATAAAGCAACAGAATTGAAGAAAATTGAGAAAAAATTAGCTCAAGAAACAGGTCTGACCAGCGAAGAATA
TATCAAATCAGCCTTAAAACTTATGATGAAATAA

Upstream 100 bases:

>100_bases
CAGATTAATGATGAAGTTGGTCAGTAAAAAAATAGATAAAAACTACTGACAGACTTGAATAAGGGAGTTTGTCAATCAAA
AAAATTAGAAAAGTAAAAAA

Downstream 100 bases:

>100_bases
AAAGTTTGAAGAAAGAACTGACAGACATCAAAAATAGTAATTCAAGTCTTTCTGTCAGTAAAATTTACTGACAGCTTCTC
ATAAGTCATCAAAAATCATC

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MFEYLNGKLVKISPTNIVIDVAGIGYLISVANPYAWSALMNTEVKIYVHQVIREDAHSLYGFVNEAEKALFLRLISVSGI
GPKSALAIIAAADNEGLITAIDNSDIKYLTKFPGVGKKTAMQMVLDLAGKFDATGTVGISLLDAGPAGNLALEEAIEALQ
ALGYKATELKKIEKKLAQETGLTSEEYIKSALKLMMK

Sequences:

>Translated_197_residues
MFEYLNGKLVKISPTNIVIDVAGIGYLISVANPYAWSALMNTEVKIYVHQVIREDAHSLYGFVNEAEKALFLRLISVSGI
GPKSALAIIAAADNEGLITAIDNSDIKYLTKFPGVGKKTAMQMVLDLAGKFDATGTVGISLLDAGPAGNLALEEAIEALQ
ALGYKATELKKIEKKLAQETGLTSEEYIKSALKLMMK
>Mature_197_residues
MFEYLNGKLVKISPTNIVIDVAGIGYLISVANPYAWSALMNTEVKIYVHQVIREDAHSLYGFVNEAEKALFLRLISVSGI
GPKSALAIIAAADNEGLITAIDNSDIKYLTKFPGVGKKTAMQMVLDLAGKFDATGTVGISLLDAGPAGNLALEEAIEALQ
ALGYKATELKKIEKKLAQETGLTSEEYIKSALKLMMK

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=201, Percent_Identity=33.8308457711443, Blast_Score=116, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_LACLA (Q9CDL2)

Other databases:

- EMBL:   AE005176
- PIR:   G86900
- RefSeq:   NP_268364.1
- ProteinModelPortal:   Q9CDL2
- SMR:   Q9CDL2
- GeneID:   1115885
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0266
- NMPDR:   fig|272623.1.peg.2262
- HOGENOM:   HBG635309
- OMA:   LFGFAQK
- ProtClustDB:   PRK00116
- BioCyc:   LLAC272623:L0266-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 21167; Mature: 21167

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEYLNGKLVKISPTNIVIDVAGIGYLISVANPYAWSALMNTEVKIYVHQVIREDAHSLY
CCCCCCCEEEEEECCEEEEEECCCEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHH
GFVNEAEKALFLRLISVSGIGPKSALAIIAAADNEGLITAIDNSDIKYLTKFPGVGKKTA
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEECCCCCEEEECCCCCCHHHH
MQMVLDLAGKFDATGTVGISLLDAGPAGNLALEEAIEALQALGYKATELKKIEKKLAQET
HHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
GLTSEEYIKSALKLMMK
CCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MFEYLNGKLVKISPTNIVIDVAGIGYLISVANPYAWSALMNTEVKIYVHQVIREDAHSLY
CCCCCCCEEEEEECCEEEEEECCCEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHH
GFVNEAEKALFLRLISVSGIGPKSALAIIAAADNEGLITAIDNSDIKYLTKFPGVGKKTA
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEECCCCCEEEECCCCCCHHHH
MQMVLDLAGKFDATGTVGISLLDAGPAGNLALEEAIEALQALGYKATELKKIEKKLAQET
HHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
GLTSEEYIKSALKLMMK
CCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471