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The map label for this gene is ruvB

Identifier: 15674188

GI number: 15674188

Start: 2288423

End: 2289397

Strand: Reverse

Name: ruvB

Synonym: L0267

Alternate gene names: 15674188

Gene position: 2289397-2288423 (Counterclockwise)

Preceding gene: 15674189

Following gene: 15674187

Centisome position: 96.78

GC content: 39.38

Gene sequence:

>975_bases
ATGGAAGAAGATTACGGTATTGAAAAATCTCTACGTCCGCAATTTTTTAATCAATATATTGGTCAAGATAAAGTCAAAGA
ACAATTAGAAATCTTTATTAAAGCAGCAAAAATGCGTGAAGAAGTTTTGGACCACGTGTTACTTTTTGGCCCTCCGGGAC
TTGGTAAGACAACAATGGCATTTGTCATTGCCAATGAGTTGGGAGTTAATATTAAACAAACAGCGGGTCCTGCCATTGAA
AAGCCAGGAGATTTAGTTGCAATTTTGAATGAATTGGAACCTGGCGATGTACTATTTATTGATGAAATTCATCGGATGCC
AATGCAAGTTGAAGAAGTTCTTTATAGCGCTATGGAAGATTTCTACATTGATATTATGCTTGGTTCTGGTGATGGTTCAC
GCTCGGTTCATTTAGATTTGCCGCCATTTACTCTAGTTGGGGCAACAACAAGAGCTGGAATGCTTTCGAATCCATTGCGT
GCCCGTTTTGGAATTTCAAGTCATATGGAGTATTATCAAGAGCGTGATTTAGAAGAAATTGTTAAAAGAACAGCTGATAT
TTTTGAAGTCGAAGTTATTGATAATGCCGCTTTAGAGATTGCTTTACGTAGTCGAGGGACTCCACGGATTGCCAATCGTT
TACTTAAACGAGTGCGTGATTTTGCTCAAATAATGGGGGATGGCCGAGTGGATAAAGCAATTACCGATAAGGCCTTGTCT
ATTCTTGATGTTGATGCCGCTGGTTTGGACTATATTGACCAAAAAATTCTCCGCACAATGATTGAAATGTACCACGGTGG
TCCTGTTGGGATTGGAACTTTGGCGGTAAATATTGCCGAAGACCGTGAAACGGTCGAGGATATGTATGAACCTTATTTGA
TTCAAAAAGGATTTTTGATGAGAACTAAACAGGGACGTAAGGTGACCCAAAGAGCCTATGAACACCTAGGGTATGTTTAC
AATGAAGAAGATTGA

Upstream 100 bases:

>100_bases
TGTCAGTAAAATTTACTGACAGCTTCTCATAAGTCATCAAAAATCATCTCTAAAAGGAGAATTACAAGATAAGATGAACG
ATATATTAAACAAAGAGCCA

Downstream 100 bases:

>100_bases
CAGTTAGCATCTGTCAGTAAAAAAGTTTGTCAAAGCTTGACGGGCTTTTTTATATTACATAAATTCCTGTCGTTGTAACA
ATAATGAAAAAAGTATATAA

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MEEDYGIEKSLRPQFFNQYIGQDKVKEQLEIFIKAAKMREEVLDHVLLFGPPGLGKTTMAFVIANELGVNIKQTAGPAIE
KPGDLVAILNELEPGDVLFIDEIHRMPMQVEEVLYSAMEDFYIDIMLGSGDGSRSVHLDLPPFTLVGATTRAGMLSNPLR
ARFGISSHMEYYQERDLEEIVKRTADIFEVEVIDNAALEIALRSRGTPRIANRLLKRVRDFAQIMGDGRVDKAITDKALS
ILDVDAAGLDYIDQKILRTMIEMYHGGPVGIGTLAVNIAEDRETVEDMYEPYLIQKGFLMRTKQGRKVTQRAYEHLGYVY
NEED

Sequences:

>Translated_324_residues
MEEDYGIEKSLRPQFFNQYIGQDKVKEQLEIFIKAAKMREEVLDHVLLFGPPGLGKTTMAFVIANELGVNIKQTAGPAIE
KPGDLVAILNELEPGDVLFIDEIHRMPMQVEEVLYSAMEDFYIDIMLGSGDGSRSVHLDLPPFTLVGATTRAGMLSNPLR
ARFGISSHMEYYQERDLEEIVKRTADIFEVEVIDNAALEIALRSRGTPRIANRLLKRVRDFAQIMGDGRVDKAITDKALS
ILDVDAAGLDYIDQKILRTMIEMYHGGPVGIGTLAVNIAEDRETVEDMYEPYLIQKGFLMRTKQGRKVTQRAYEHLGYVY
NEED
>Mature_324_residues
MEEDYGIEKSLRPQFFNQYIGQDKVKEQLEIFIKAAKMREEVLDHVLLFGPPGLGKTTMAFVIANELGVNIKQTAGPAIE
KPGDLVAILNELEPGDVLFIDEIHRMPMQVEEVLYSAMEDFYIDIMLGSGDGSRSVHLDLPPFTLVGATTRAGMLSNPLR
ARFGISSHMEYYQERDLEEIVKRTADIFEVEVIDNAALEIALRSRGTPRIANRLLKRVRDFAQIMGDGRVDKAITDKALS
ILDVDAAGLDYIDQKILRTMIEMYHGGPVGIGTLAVNIAEDRETVEDMYEPYLIQKGFLMRTKQGRKVTQRAYEHLGYVY
NEED

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=315, Percent_Identity=57.4603174603175, Blast_Score=389, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_LACLA (Q9CDL3)

Other databases:

- EMBL:   AE005176
- PIR:   F86900
- RefSeq:   NP_268363.1
- ProteinModelPortal:   Q9CDL3
- SMR:   Q9CDL3
- GeneID:   1115884
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0267
- NMPDR:   fig|272623.1.peg.2261
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- ProtClustDB:   PRK00080
- BioCyc:   LLAC272623:L0267-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 36579; Mature: 36579

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEDYGIEKSLRPQFFNQYIGQDKVKEQLEIFIKAAKMREEVLDHVLLFGPPGLGKTTMA
CCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
FVIANELGVNIKQTAGPAIEKPGDLVAILNELEPGDVLFIDEIHRMPMQVEEVLYSAMED
HHHHHHHCCCCHHCCCCCCCCCCHHHHHHHCCCCCCEEEHHHHHCCCHHHHHHHHHHHHH
FYIDIMLGSGDGSRSVHLDLPPFTLVGATTRAGMLSNPLRARFGISSHMEYYQERDLEEI
EEEEEEEECCCCCCEEEEECCCEEEECCCHHCCHHCCCHHHHHCHHHHHHHHHHCCHHHH
VKRTADIFEVEVIDNAALEIALRSRGTPRIANRLLKRVRDFAQIMGDGRVDKAITDKALS
HHHHHHHEEEEEECCHHHEEHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ILDVDAAGLDYIDQKILRTMIEMYHGGPVGIGTLAVNIAEDRETVEDMYEPYLIQKGFLM
HEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEHHHHHHHHHHHHHHHHCCCHHH
RTKQGRKVTQRAYEHLGYVYNEED
CCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MEEDYGIEKSLRPQFFNQYIGQDKVKEQLEIFIKAAKMREEVLDHVLLFGPPGLGKTTMA
CCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
FVIANELGVNIKQTAGPAIEKPGDLVAILNELEPGDVLFIDEIHRMPMQVEEVLYSAMED
HHHHHHHCCCCHHCCCCCCCCCCHHHHHHHCCCCCCEEEHHHHHCCCHHHHHHHHHHHHH
FYIDIMLGSGDGSRSVHLDLPPFTLVGATTRAGMLSNPLRARFGISSHMEYYQERDLEEI
EEEEEEEECCCCCCEEEEECCCEEEECCCHHCCHHCCCHHHHHCHHHHHHHHHHCCHHHH
VKRTADIFEVEVIDNAALEIALRSRGTPRIANRLLKRVRDFAQIMGDGRVDKAITDKALS
HHHHHHHEEEEEECCHHHEEHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ILDVDAAGLDYIDQKILRTMIEMYHGGPVGIGTLAVNIAEDRETVEDMYEPYLIQKGFLM
HEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEHHHHHHHHHHHHHHHHCCCHHH
RTKQGRKVTQRAYEHLGYVYNEED
CCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471