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The map label for this gene is als

Identifier: 15673166

GI number: 15673166

Start: 1200450

End: 1202114

Strand: Direct

Name: als

Synonym: L210

Alternate gene names: 15673166

Gene position: 1200450-1202114 (Clockwise)

Preceding gene: 15673165

Following gene: 15673168

Centisome position: 50.75

GC content: 40.6

Gene sequence:

>1665_bases
ATGTCTGAGAAACAATTTGGGGCGAACTTGGTTGTCGATAGTTTGATTAACCATAAAGTGAAGTATGTATTTGGGATTCC
AGGAGCAAAAATTGACCGGGTTTTTGATTTATTAGAAAATGAAGAAGGCCCTCAAATGGTCGTGACTCGTCATGAGCAAG
GAGCTGCTTTCATGGCTCAAGCTGTCGGTCGTTTAACTGGCGAACCTGGTGTAGTAGTTGTTACGAGTGGGCCTGGTGTA
TCAAACCTTGCGACTCCGCTTTTGACCGCGACATCAGAAGGTGATGCTATTTTGGCTATCGGTGGACAAGTTAAACGAAG
TGACCGTCTTAAACGTGCGCACCAATCAATGGATAATGCTGGAATGATGCAATCAGCAACAAAATATTCAGCAGAAGTTC
TTGACCCTAATACACTTTCTGAATCAATTGCCAACGCTTATCGTATTGCAAAATCAGGACATCCAGGTGCAACTTTCTTA
TCAATCCCCCAAGATGTAACGGATGCCGAAGTATCAATCAAAGCCATTCAACCACTTTCAGACCCTAAAATGGGGAATGC
CTCTATTGATGACATTAATTATTTAGCACAAGCAATTAAAAATGCTGTATTGCCAGTAATTTTGGTTGGAGCTGGTGCTT
CAGATGCTAAAGTCGCTTCATCCTTGCGTAATCTATTGACTCATGTTAATATTCCTGTCGTTGAAACATTCCAAGGTGCA
GGGGTTATTTCACATGATTTAGAACATACTTTTTATGGACGTATCGGTCTTTTCCGCAATCAACCAGGCGATATGCTTCT
GAAACGTTCTGACCTTGTTATTGCTGTTGGTTATGACCCAATTGAATATGAAGCTCGTAACTGGAATGCAGAAATTGATA
GTCGAATTATCGTTATTGATAATGCCATTGCTGAAATTGATACTTACTACCAACCAGAGCGTGAATTAATTGGTGATATC
GCAGCAACATTGGATAATCTTTTACCAGCTGTTCGTGGCTACAAAATTCCAAAAGGAACAAAAGATTATCTCGATGGCCT
TCATGAAGTTGCTGAGCAACACGAATTTGATACTGAAAATACTGAAGAAGGTAGAATGCACCCTCTTGATTTGGTCAGCA
CTTTCCAAGAAATCGTCAAGGATGATGAAACAGTAACCGTTGACGTAGGTTCACTCTACATTTGGATGGCACGTCATTTC
AAATCATACGAACCACGTCATCTCCTCTTCTCAAACGGAATGCAAACACTCGGAGTTGCACTTCCTTGGGCAATTACAGC
CGCATTGTTGCGCCCAGGTAAAAAAGTTTATTCACACTCTGGTGATGGAGGCTTCCTTTTCACAGGGCAAGAATTGGAAA
CAGCTGTACGTTTGAATCTTCCAATCGTTCAAATTATCTGGAATGACGGCCATTATGATATGGTTAAATTCCAAGAAGAA
ATGAAATATGGTCGTTCAGCAGCCGTTGATTTTGGCTATGTTGATTACGTAAAATATGCTGAAGCAATGAGAGCAAAAGG
TTACCGTGCACACAGCAAAGAAGAACTTGCTGAAATTCTCAAATCAATCCCAGATACTACTGGACCGGTGGTAATTGACG
TTCCTTTGGACTATTCTGATAACATTAAATTAGCAGAAAAATTATTGCCTGAAGAGTTTTATTGA

Upstream 100 bases:

>100_bases
CAAAATAATTGTAAAAGGTTCTATTATCTGATAAAATGATTGTGAAGTAATCCAAGAGATTATGAAATATGAATTAGAAC
AAATAGAGGTAAAATAAAAA

Downstream 100 bases:

>100_bases
TTACAATCAAGCAATTTGTGGCATAACAAAATAAAAGAAGAAGGCCTTGAACACCTAAGCGTTCAGGGCCTTTTTTTGTG
AAATAAATTAGATGAAATTT

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MSEKQFGANLVVDSLINHKVKYVFGIPGAKIDRVFDLLENEEGPQMVVTRHEQGAAFMAQAVGRLTGEPGVVVVTSGPGV
SNLATPLLTATSEGDAILAIGGQVKRSDRLKRAHQSMDNAGMMQSATKYSAEVLDPNTLSESIANAYRIAKSGHPGATFL
SIPQDVTDAEVSIKAIQPLSDPKMGNASIDDINYLAQAIKNAVLPVILVGAGASDAKVASSLRNLLTHVNIPVVETFQGA
GVISHDLEHTFYGRIGLFRNQPGDMLLKRSDLVIAVGYDPIEYEARNWNAEIDSRIIVIDNAIAEIDTYYQPERELIGDI
AATLDNLLPAVRGYKIPKGTKDYLDGLHEVAEQHEFDTENTEEGRMHPLDLVSTFQEIVKDDETVTVDVGSLYIWMARHF
KSYEPRHLLFSNGMQTLGVALPWAITAALLRPGKKVYSHSGDGGFLFTGQELETAVRLNLPIVQIIWNDGHYDMVKFQEE
MKYGRSAAVDFGYVDYVKYAEAMRAKGYRAHSKEELAEILKSIPDTTGPVVIDVPLDYSDNIKLAEKLLPEEFY

Sequences:

>Translated_554_residues
MSEKQFGANLVVDSLINHKVKYVFGIPGAKIDRVFDLLENEEGPQMVVTRHEQGAAFMAQAVGRLTGEPGVVVVTSGPGV
SNLATPLLTATSEGDAILAIGGQVKRSDRLKRAHQSMDNAGMMQSATKYSAEVLDPNTLSESIANAYRIAKSGHPGATFL
SIPQDVTDAEVSIKAIQPLSDPKMGNASIDDINYLAQAIKNAVLPVILVGAGASDAKVASSLRNLLTHVNIPVVETFQGA
GVISHDLEHTFYGRIGLFRNQPGDMLLKRSDLVIAVGYDPIEYEARNWNAEIDSRIIVIDNAIAEIDTYYQPERELIGDI
AATLDNLLPAVRGYKIPKGTKDYLDGLHEVAEQHEFDTENTEEGRMHPLDLVSTFQEIVKDDETVTVDVGSLYIWMARHF
KSYEPRHLLFSNGMQTLGVALPWAITAALLRPGKKVYSHSGDGGFLFTGQELETAVRLNLPIVQIIWNDGHYDMVKFQEE
MKYGRSAAVDFGYVDYVKYAEAMRAKGYRAHSKEELAEILKSIPDTTGPVVIDVPLDYSDNIKLAEKLLPEEFY
>Mature_553_residues
SEKQFGANLVVDSLINHKVKYVFGIPGAKIDRVFDLLENEEGPQMVVTRHEQGAAFMAQAVGRLTGEPGVVVVTSGPGVS
NLATPLLTATSEGDAILAIGGQVKRSDRLKRAHQSMDNAGMMQSATKYSAEVLDPNTLSESIANAYRIAKSGHPGATFLS
IPQDVTDAEVSIKAIQPLSDPKMGNASIDDINYLAQAIKNAVLPVILVGAGASDAKVASSLRNLLTHVNIPVVETFQGAG
VISHDLEHTFYGRIGLFRNQPGDMLLKRSDLVIAVGYDPIEYEARNWNAEIDSRIIVIDNAIAEIDTYYQPERELIGDIA
ATLDNLLPAVRGYKIPKGTKDYLDGLHEVAEQHEFDTENTEEGRMHPLDLVSTFQEIVKDDETVTVDVGSLYIWMARHFK
SYEPRHLLFSNGMQTLGVALPWAITAALLRPGKKVYSHSGDGGFLFTGQELETAVRLNLPIVQIIWNDGHYDMVKFQEEM
KYGRSAAVDFGYVDYVKYAEAMRAKGYRAHSKEELAEILKSIPDTTGPVVIDVPLDYSDNIKLAEKLLPEEFY

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=574, Percent_Identity=27.5261324041812, Blast_Score=190, Evalue=3e-48,
Organism=Homo sapiens, GI21361361, Length=584, Percent_Identity=23.458904109589, Blast_Score=142, Evalue=6e-34,
Organism=Escherichia coli, GI1790104, Length=554, Percent_Identity=29.7833935018051, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI87081685, Length=558, Percent_Identity=27.9569892473118, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI1786717, Length=558, Percent_Identity=24.3727598566308, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1787096, Length=545, Percent_Identity=25.8715596330275, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788716, Length=552, Percent_Identity=25, Blast_Score=137, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17531301, Length=550, Percent_Identity=27.0909090909091, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17531299, Length=550, Percent_Identity=27.0909090909091, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=549, Percent_Identity=24.7723132969035, Blast_Score=125, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=560, Percent_Identity=28.75, Blast_Score=203, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6320816, Length=474, Percent_Identity=23.2067510548523, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922626, Length=561, Percent_Identity=25.3119429590018, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60864; Mature: 60733

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKQFGANLVVDSLINHKVKYVFGIPGAKIDRVFDLLENEEGPQMVVTRHEQGAAFMAQ
CCCCHHCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHH
AVGRLTGEPGVVVVTSGPGVSNLATPLLTATSEGDAILAIGGQVKRSDRLKRAHQSMDNA
HHHHHCCCCCEEEEECCCCCHHHHHHHEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCC
GMMQSATKYSAEVLDPNTLSESIANAYRIAKSGHPGATFLSIPQDVTDAEVSIKAIQPLS
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEECCCC
DPKMGNASIDDINYLAQAIKNAVLPVILVGAGASDAKVASSLRNLLTHVNIPVVETFQGA
CCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCEECCCCC
GVISHDLEHTFYGRIGLFRNQPGDMLLKRSDLVIAVGYDPIEYEARNWNAEIDSRIIVID
CCHHHHHHHHHHHCEEEECCCCCCCEEEECCEEEEECCCCCEECCCCCCCCCCCEEEEEE
NAIAEIDTYYQPERELIGDIAATLDNLLPAVRGYKIPKGTKDYLDGLHEVAEQHEFDTEN
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCC
TEEGRMHPLDLVSTFQEIVKDDETVTVDVGSLYIWMARHFKSYEPRHLLFSNGMQTLGVA
CCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHH
LPWAITAALLRPGKKVYSHSGDGGFLFTGQELETAVRLNLPIVQIIWNDGHYDMVKFQEE
HHHHHHHHHHCCCHHHHCCCCCCCEEEECHHHCEEEEECCCEEEEEECCCCCHHHHHHHH
MKYGRSAAVDFGYVDYVKYAEAMRAKGYRAHSKEELAEILKSIPDTTGPVVIDVPLDYSD
HHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCC
NIKLAEKLLPEEFY
CCHHHHHCCCCCCC
>Mature Secondary Structure 
SEKQFGANLVVDSLINHKVKYVFGIPGAKIDRVFDLLENEEGPQMVVTRHEQGAAFMAQ
CCCHHCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHH
AVGRLTGEPGVVVVTSGPGVSNLATPLLTATSEGDAILAIGGQVKRSDRLKRAHQSMDNA
HHHHHCCCCCEEEEECCCCCHHHHHHHEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCC
GMMQSATKYSAEVLDPNTLSESIANAYRIAKSGHPGATFLSIPQDVTDAEVSIKAIQPLS
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEECCCC
DPKMGNASIDDINYLAQAIKNAVLPVILVGAGASDAKVASSLRNLLTHVNIPVVETFQGA
CCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCEECCCCC
GVISHDLEHTFYGRIGLFRNQPGDMLLKRSDLVIAVGYDPIEYEARNWNAEIDSRIIVID
CCHHHHHHHHHHHCEEEECCCCCCCEEEECCEEEEECCCCCEECCCCCCCCCCCEEEEEE
NAIAEIDTYYQPERELIGDIAATLDNLLPAVRGYKIPKGTKDYLDGLHEVAEQHEFDTEN
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCC
TEEGRMHPLDLVSTFQEIVKDDETVTVDVGSLYIWMARHFKSYEPRHLLFSNGMQTLGVA
CCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHH
LPWAITAALLRPGKKVYSHSGDGGFLFTGQELETAVRLNLPIVQIIWNDGHYDMVKFQEE
HHHHHHHHHHCCCHHHHCCCCCCCEEEECHHHCEEEEECCCEEEEEECCCCCHHHHHHHH
MKYGRSAAVDFGYVDYVKYAEAMRAKGYRAHSKEELAEILKSIPDTTGPVVIDVPLDYSD
HHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCC
NIKLAEKLLPEEFY
CCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]