Click here to switch to the map view.

The map label for this gene is mae

Identifier: 15673168

GI number: 15673168

Start: 1203477

End: 1204619

Strand: Direct

Name: mae

Synonym: L3227

Alternate gene names: 15673168

Gene position: 1203477-1204619 (Clockwise)

Preceding gene: 15673166

Following gene: 15673170

Centisome position: 50.87

GC content: 40.16

Gene sequence:

>1143_bases
ATGGTTGATTTTAATAAAGTTTTAGATTTACACGAATTACACACAGGTGTATTGGATATTCAAGCAGACATTGATGTCAA
AAAGCCAAGTGATCTGGCTGAGGCTTATACACCGGGTGTTGCCGAACTTTCAAAATTAATTCAGAGTGACCCAGAAGCTA
AACGCAAATACACAATGAGCGGAAAACTGGTGGCCGTTGTGACTGATGGAACAGCTGTTCTTGGTCTTGGAAATATGGGG
CCTGCTGGTGGACTCCCTATCGTTGAGGGGAAAGCCCTTCTGTACAAGGAATTAGCTGGTGTCGATGCAATACCGATTGC
TGTGGAACAAATCGGTGCTGAAAAAATCATCGAAACAATTAAAAATATTTCGCAATCTTTTGCTGGTATTCACTTGGAAG
ATATCTCAGCTCCTCAATGTTTTGAAATTGAGGAACGACTTTCTAAAAATTTGAATATTCCAGTTTATCATGATGACCAA
GCTGGGACAGCTATCGTTGTCTTAGCAGGTTTGATGAATGCAGCCAAGTTAGTTAATAAGAAATTGGAAGACTTGAAGAT
TGTCATCAATGGTGTTGGTGCAGCTGGTGTTGCAACCGGGCGATTATTTGCTGAGGTTGGCATTAAAAATGTGACCTTGA
TTGATATTGAAGGAAAAGTTAACGATAAGGTGAAATCTTATAATCGTTATCAGTTGGAATTGGCTAAACGCTTTAATCAA
GTAGCTGGGACGACTTTGGATGAAGTGATTGACGGTCAAGATGTCTTTATTGGTTTGTCAGATGCCAAAGTTTTGACTGG
GACACAAGTTGAACGCATGGCAAAAGATGCAATTATCTTTGCTTTAGCAAATCCAGTGCCAGAAATCTTACCAGAAGAAG
CGCTCAAGCATGGAGCTGCAGTTGTTGCAACAGGCTCTAATCAATGGCCAAATCAAGTCAATAATGTTTTGGTGTTCCCT
GGCTTGTTTAAAGGTTTATTGAGTGCGAATGTTCAGCACGTAGATTACAAGTTGCAAGCTAATATTGCGACAGCCATTGC
TGATGTCATTAAAAATCCAGATCGGAATCACATTGTTCCTGGGGTATTTGATGCAGGAGTTGTAGAAAGTGTCACAAAAG
CGGTAAAAGATTCAGCAAAATAA

Upstream 100 bases:

>100_bases
ATATTTTAAAATATTTAATATAAATATTTGACATTTTTTAAATAGAAGAGTATAACTTAGAATGTAGGTTACAAATAAAA
GTTATTAGGAAAGAAGAAAA

Downstream 100 bases:

>100_bases
AAAAACTTTTATCGTAGTAAGCTATGGTTACAAAATTTATATAATAAAGGAGAAGACGAATGGATGTCTTTTGGACTTCA
GTACAATCTGTACTGATGAT

Product: malate oxidoreductase

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MVDFNKVLDLHELHTGVLDIQADIDVKKPSDLAEAYTPGVAELSKLIQSDPEAKRKYTMSGKLVAVVTDGTAVLGLGNMG
PAGGLPIVEGKALLYKELAGVDAIPIAVEQIGAEKIIETIKNISQSFAGIHLEDISAPQCFEIEERLSKNLNIPVYHDDQ
AGTAIVVLAGLMNAAKLVNKKLEDLKIVINGVGAAGVATGRLFAEVGIKNVTLIDIEGKVNDKVKSYNRYQLELAKRFNQ
VAGTTLDEVIDGQDVFIGLSDAKVLTGTQVERMAKDAIIFALANPVPEILPEEALKHGAAVVATGSNQWPNQVNNVLVFP
GLFKGLLSANVQHVDYKLQANIATAIADVIKNPDRNHIVPGVFDAGVVESVTKAVKDSAK

Sequences:

>Translated_380_residues
MVDFNKVLDLHELHTGVLDIQADIDVKKPSDLAEAYTPGVAELSKLIQSDPEAKRKYTMSGKLVAVVTDGTAVLGLGNMG
PAGGLPIVEGKALLYKELAGVDAIPIAVEQIGAEKIIETIKNISQSFAGIHLEDISAPQCFEIEERLSKNLNIPVYHDDQ
AGTAIVVLAGLMNAAKLVNKKLEDLKIVINGVGAAGVATGRLFAEVGIKNVTLIDIEGKVNDKVKSYNRYQLELAKRFNQ
VAGTTLDEVIDGQDVFIGLSDAKVLTGTQVERMAKDAIIFALANPVPEILPEEALKHGAAVVATGSNQWPNQVNNVLVFP
GLFKGLLSANVQHVDYKLQANIATAIADVIKNPDRNHIVPGVFDAGVVESVTKAVKDSAK
>Mature_380_residues
MVDFNKVLDLHELHTGVLDIQADIDVKKPSDLAEAYTPGVAELSKLIQSDPEAKRKYTMSGKLVAVVTDGTAVLGLGNMG
PAGGLPIVEGKALLYKELAGVDAIPIAVEQIGAEKIIETIKNISQSFAGIHLEDISAPQCFEIEERLSKNLNIPVYHDDQ
AGTAIVVLAGLMNAAKLVNKKLEDLKIVINGVGAAGVATGRLFAEVGIKNVTLIDIEGKVNDKVKSYNRYQLELAKRFNQ
VAGTTLDEVIDGQDVFIGLSDAKVLTGTQVERMAKDAIIFALANPVPEILPEEALKHGAAVVATGSNQWPNQVNNVLVFP
GLFKGLLSANVQHVDYKLQANIATAIADVIKNPDRNHIVPGVFDAGVVESVTKAVKDSAK

Specific function: In addition to the NAD-dependent oxidative decarboxylation of L-malate, the enzyme catalyzes the decarboxylation of oxaloacetate [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=350, Percent_Identity=26.2857142857143, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788806, Length=394, Percent_Identity=38.3248730964467, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI87081919, Length=336, Percent_Identity=26.1904761904762, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17537199, Length=354, Percent_Identity=25.4237288135593, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322823, Length=321, Percent_Identity=27.7258566978193, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI21356279, Length=361, Percent_Identity=24.9307479224377, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI281362674, Length=361, Percent_Identity=24.9307479224377, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI281362672, Length=361, Percent_Identity=24.9307479224377, Blast_Score=86, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 40476; Mature: 40476

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDFNKVLDLHELHTGVLDIQADIDVKKPSDLAEAYTPGVAELSKLIQSDPEAKRKYTMS
CCCHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHCCCHHHHEEEEC
GKLVAVVTDGTAVLGLGNMGPAGGLPIVEGKALLYKELAGVDAIPIAVEQIGAEKIIETI
CCEEEEEECCEEEEEECCCCCCCCCCEECCHHHHHHHHCCCCCHHHHHHHHCHHHHHHHH
KNISQSFAGIHLEDISAPQCFEIEERLSKNLNIPVYHDDQAGTAIVVLAGLMNAAKLVNK
HHHHHHHCCCEEECCCCCCCCHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHH
KLEDLKIVINGVGAAGVATGRLFAEVGIKNVTLIDIEGKVNDKVKSYNRYQLELAKRFNQ
HHHHHEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCHHHHHHHHHHHH
VAGTTLDEVIDGQDVFIGLSDAKVLTGTQVERMAKDAIIFALANPVPEILPEEALKHGAA
HHCCCHHHHHCCCEEEEEECCCEEECCHHHHHHHHHHEEEEECCCCHHHCHHHHHHCCCE
VVATGSNQWPNQVNNVLVFPGLFKGLLSANVQHVDYKLQANIATAIADVIKNPDRNHIVP
EEEECCCCCCHHHCCEEECHHHHHHHHHCCCHHEEEEEHHHHHHHHHHHHCCCCCCCCCC
GVFDAGVVESVTKAVKDSAK
CCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVDFNKVLDLHELHTGVLDIQADIDVKKPSDLAEAYTPGVAELSKLIQSDPEAKRKYTMS
CCCHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHCCCHHHHEEEEC
GKLVAVVTDGTAVLGLGNMGPAGGLPIVEGKALLYKELAGVDAIPIAVEQIGAEKIIETI
CCEEEEEECCEEEEEECCCCCCCCCCEECCHHHHHHHHCCCCCHHHHHHHHCHHHHHHHH
KNISQSFAGIHLEDISAPQCFEIEERLSKNLNIPVYHDDQAGTAIVVLAGLMNAAKLVNK
HHHHHHHCCCEEECCCCCCCCHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHH
KLEDLKIVINGVGAAGVATGRLFAEVGIKNVTLIDIEGKVNDKVKSYNRYQLELAKRFNQ
HHHHHEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCHHHHHHHHHHHH
VAGTTLDEVIDGQDVFIGLSDAKVLTGTQVERMAKDAIIFALANPVPEILPEEALKHGAA
HHCCCHHHHHCCCEEEEEECCCEEECCHHHHHHHHHHEEEEECCCCHHHCHHHHHHCCCE
VVATGSNQWPNQVNNVLVFPGLFKGLLSANVQHVDYKLQANIATAIADVIKNPDRNHIVP
EEEECCCCCCHHHCCEEECHHHHHHHHHCCCHHEEEEEHHHHHHHHHHHHCCCCCCCCCC
GVFDAGVVESVTKAVKDSAK
CCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644282 [H]